BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,174,183	T→C	111 (TAA→TAG)	ygiZ ←	DUF2645 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,174,183	0	T	C	99.4%	558.8 / NA	170	111 (TAA→TAG)	ygiZ	DUF2645 family protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (43/126); minor base G (1/0); total (44/126)
Fisher's exact test for biased strand distribution p-value = 2.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 170 total aligned reads displayed.

GATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAATAAGTGGC  >  NZ_CP025268/3174047‑3174318

gATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTaa                                                                                                                                      <  1:3148829/140‑1 (MQ=255)

gATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTaa                                                                                                                                      <  2:598313/140‑1 (MQ=255)

    gTCCAGCGAATCGCCAAATTGCATGGCATGAACGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGc                                                                                                                                  <  2:4421159/140‑1 (MQ=255)

     tCCAGCGACTTGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGAAACAAGGTGGATTTGGGGCAAAGGTAACCTGGTAAATTCTCTGTCTAAGAGAGCCGTTAAATCGACTCTCATAGATTTTACTAACAGCa                                                                                                                                 <  2:205595/140‑1 (MQ=255)

     tCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                                                                                 <  2:3057542/140‑1 (MQ=255)

     tCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                                                                                 <  1:2778724/140‑1 (MQ=255)

     tCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                                                                                 <  2:464893/140‑1 (MQ=255)

     tCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                                                                                 <  2:4181821/140‑1 (MQ=255)

     tCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                                                                                 <  1:1825734/140‑1 (MQ=255)

     tCCAGCGAATCGCCAAATTGCATGGAATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAAGTAAGCTGGTACCTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                                                                                 <  2:887269/140‑1 (MQ=255)

     tCCAGCGAATCGCAAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                                                                                 <  2:156017/140‑1 (MQ=255)

     tCCAGCCAAGCGACAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGGAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCa                                                                                                                                 <  2:1955057/140‑1 (MQ=255)

      ccAGCGAATCGCCCAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCaa                                                                                                                                <  1:676040/140‑1 (MQ=255)

      ccAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCaa                                                                                                                                <  2:3995076/140‑1 (MQ=255)

      ccAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCaa                                                                                                                                <  2:4033063/140‑1 (MQ=255)

      ccAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCaa                                                                                                                                <  2:3165879/140‑1 (MQ=255)

      ccAGCGAATCGCAAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCaa                                                                                                                                <  1:1358119/140‑1 (MQ=255)

            aaTCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAAtata                                                                                                                          >  1:3604085/1‑140 (MQ=255)

               cGCCAAATTGCAGGGCATGACTGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAAc                                                                                                                       >  2:5281604/1‑140 (MQ=255)

                  cAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGt                                                                                                                    <  2:4348221/140‑1 (MQ=255)

                                tgAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGcca                                                                                                      >  1:1043719/1‑140 (MQ=255)

                                  aaTGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGccacc                                                                                                    <  2:1307819/140‑1 (MQ=255)

                                  aaTGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGccacc                                                                                                    <  1:364089/140‑1 (MQ=255)

                                  aaTGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGccacc                                                                                                    <  2:1307033/140‑1 (MQ=255)

                                  aaTGTTGAATTTGGGAATGCCGAACAAGGTCGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGccacc                                                                                                    <  1:1282722/140‑1 (MQ=255)

                                       tGAATTTGGGAATGCGGAATAAGGTGGATTTGCGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCaccaacca                                                                                               <  2:4127791/140‑1 (MQ=255)

                                        gAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGATTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcac                                                                                              >  1:4459938/1‑140 (MQ=255)

                                        gAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcac                                                                                              >  1:2555726/1‑140 (MQ=255)

                                        gAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcac                                                                                              >  2:1045973/1‑140 (MQ=255)

                                         aaTTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACcaca                                                                                             <  2:5339950/140‑1 (MQ=255)

                                            ttGGGAATGCTGAATAAGGGGGATTTGAGTCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                          <  2:3957798/140‑1 (MQ=255)

                                            ttGGGAATGCGGAACAAGGTGGATTTGAGGCGAATGTAAGCTGGTAACTTCTCTGTCTATCAGAGCCGTTAAAACGACGCTCATAGATTTTACTCCTCGCAAAATATAAACCGTCCCCAAAACAGCCCCCAACCTCTAAc                                                                                          <  2:5272960/140‑1 (MQ=255)

                                            ttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTCAAACGACTCTCATAGCTTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                          <  1:2052445/140‑1 (MQ=255)

                                            ttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTACTAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCacacac                                                                                          <  2:4305619/140‑1 (MQ=255)

                                            ttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                          <  2:5243210/140‑1 (MQ=255)

                                            ttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                          <  1:1456401/140‑1 (MQ=255)

                                            ttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                          <  2:1789223/140‑1 (MQ=255)

                                            ttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                          <  2:4726523/140‑1 (MQ=255)

                                            ttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                          <  1:3620393/140‑1 (MQ=255)

                                            ttGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                          <  1:2862165/140‑1 (MQ=255)

                                            ttGGGAATGCGGAACAAGGTGGATGTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATACACCGTCCCCAAAAAAGCCACCAACCACAAAc                                                                                          <  2:4156950/140‑1 (MQ=255)

                                             tGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACGGTCCCCAAAAAAGCCACCCACCACAAAcc                                                                                         >  2:1250676/1‑140 (MQ=255)

                                                     cGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACaaaa                                                                                 >  1:427412/1‑139 (MQ=255)

                                                      ggAACACGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGGATATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATa                                                                                <  2:3057313/140‑1 (MQ=255)

                                                         aCAAGGTGGATTTGATGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGGCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcg                                                                             <  2:3000626/140‑1 (MQ=255)

                                                         aCAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcg                                                                             <  1:129033/140‑1 (MQ=255)

                                                           aacgcggATTTTAGGCGAAGGTAAGCTGGTAACTTCTCCGCCTCTGAGAGCCGTTAAAACGACCCTCATAGATTTTCCTAATAGCAAAATATAAAACGTCCCCAAAAAAGCCACCAACCACAAACCAGCCAAATACcgag                                                                           <  2:4221858/135‑1 (MQ=255)

                                                           aaGGTGGATTTGAGGCGAATGTAAGCTGGTAACTTCTCTGTCTATGAGATCCGTTAAAACGAAGCGCATAGATTTTACTAATAGCAAAATATCAACCATCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                           <  2:1326257/140‑1 (MQ=255)

                                                           aaGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                           <  2:1828204/140‑1 (MQ=255)

                                                           aaGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                           <  2:5075904/140‑1 (MQ=255)

                                                           aaGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                           <  1:17018/140‑1 (MQ=255)

                                                           aaGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                           <  1:570927/140‑1 (MQ=255)

                                                           aaGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                           <  1:5328848/140‑1 (MQ=255)

                                                           aaGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                           <  1:4804643/140‑1 (MQ=255)

                                                           aaGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                           <  1:237585/140‑1 (MQ=255)

                                                           aaGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                           <  1:2411827/140‑1 (MQ=255)

                                                           aaGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgag                                                                           <  1:2988895/140‑1 (MQ=255)

                                                           aaGGTGGAGTTGAGGCGAAGGTAAGCTGGGAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTATTAGCAGAATATAAGCCGGCCCCGAAAAGGCCACCAACCACAAACCAGACAAATCCcgag                                                                           <  2:242691/140‑1 (MQ=255)

                                                            aGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagc                                                                          <  1:1131167/140‑1 (MQ=255)

                                                             ggTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcg                                                                         <  1:84397/140‑1 (MQ=255)

                                                             ggTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcg                                                                         <  2:4816199/140‑1 (MQ=255)

                                                             ggTGGATTTCAGGCCAAGGTAAGATCGTACATTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGCTTTTACTAATATCAAAATATAAACCGTTCCCAAAAGACCCACCAAACTCATACCAGACAAATACcgagcg                                                                         <  2:4856221/140‑1 (MQ=255)

                                                               tGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcgag                                                                       <  1:1045973/140‑1 (MQ=255)

                                                               tGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACcgagcgag                                                                       <  1:4000899/140‑1 (MQ=255)

                                                                 gATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGaa                                                                     <  1:3097996/140‑1 (MQ=255)

                                                                 gATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGaa                                                                     <  1:402512/140‑1 (MQ=255)

                                                                 gATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGaa                                                                     <  1:5299118/140‑1 (MQ=255)

                                                                 gATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGaa                                                                     <  1:2230533/140‑1 (MQ=255)

                                                                 gATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGaa                                                                     <  2:1550766/140‑1 (MQ=255)

                                                                  aTTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAAt                                                                    >  2:2362037/1‑140 (MQ=255)

                                                                  aTTAGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAAt                                                                    >  2:354375/1‑140 (MQ=255)

                                                                       aGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACata                                                               >  2:229557/1‑140 (MQ=255)

                                                                       aGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACata                                                               >  1:1769278/1‑140 (MQ=255)

                                                                                       gTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAAc                                               >  1:97427/1‑140 (MQ=255)

                                                                                       gTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAAc                                               >  1:3925402/1‑140 (MQ=255)

                                                                                         aaCTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACAt                                             <  1:1897826/140‑1 (MQ=255)

                                                                                           cTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACAttt                                           <  2:1042641/140‑1 (MQ=255)

                                                                                             tctcTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTcc                                         >  2:1167947/1‑140 (MQ=255)

                                                                                             tctcTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTcc                                         <  1:1824682/140‑1 (MQ=255)

                                                                                             tctcTGTCTATGAGAACAGTTAAAACGACTCTCATAGAATTTACTAATAGAAAAATATAAACCTTCCCCAAAAAAGCCACCAACCCCAAACCAGACAAATACCGAGCGAGAATACATATACCCAAATCTCGAACATTTcc                                         <  1:4950413/140‑1 (MQ=255)

                                                                                                 tGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACAGATACCCAAAACTCGAACATTTCCCGCa                                     >  1:880903/1‑140 (MQ=255)

                                                                                                   tctagtcgagCCGTTAAAACGACTCTCATATATTTTCCTAATAGCAAACTATCAACCGTCCCCAAAAAAGCCACCCACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATa                                   <  2:4703699/133‑1 (MQ=255)

                                                                                                   tCTATCAGAGCCGTTAAAACGCCTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCGACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATa                                   <  2:4703955/140‑1 (MQ=255)

                                                                                                      aTGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAag                                >  1:3003687/1‑140 (MQ=255)

                                                                                                      aTGAGAGCCGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAag                                >  1:1654454/1‑140 (MQ=255)

                                                                                                             ccGTTAAAACGAGTGGCATAGATTTTAGTGACAAAAAAATATAAACCGTCCCCAAAAAAGCGACCAACCACTAACCAGACAAATACCTGGCGAGAATACATATACGCAAAACTCGAACATTTCATGCATAAAGAGTGTTc                         >  2:4085681/1‑140 (MQ=255)

                                                                                                             ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTc                         >  2:672018/1‑140 (MQ=255)

                                                                                                             ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTc                         >  1:4341093/1‑140 (MQ=255)

                                                                                                             ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTc                         >  1:4939948/1‑140 (MQ=255)

                                                                                                             ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTc                         >  2:2315167/1‑140 (MQ=255)

                                                                                                             ccGTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTc                         >  2:2561236/1‑140 (MQ=255)

                                                                                                               gTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCtt                       >  1:629287/1‑140 (MQ=255)

                                                                                                               gTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCtt                       <  2:585427/140‑1 (MQ=255)

                                                                                                               gTTAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCtt                       <  2:585754/140‑1 (MQ=255)

                                                                                                                ttAAAACGACTCTCATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTta                      >  2:805629/1‑140 (MQ=255)

                                                                                                                         cgctcATAGATTTGACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCACCCACAAACCAGACAAATACCGAGCGAGAATACACATACCCAAAACTCAAACATTTCCCGCATAAAGAGGTTTCTTAAGATAAGaa             >  2:2165364/3‑140 (MQ=255)

                                                                                                                             cATAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGaataat         <  2:899051/140‑1 (MQ=255)

                                                                                                                               tAGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAAtaataa       <  1:20422/140‑1 (MQ=255)

                                                                                                                                aGATTTTACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAATAAg      >  1:3511648/1‑140 (MQ=255)

                                                                                                                                    tttACTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAATAAGTGGc  >  2:243527/1‑140 (MQ=255)

GATTGTCCAGCGAATCGCCAAATTGCATGGCATGAATGTTGAATTTGGGAATGCGGAACAAGGTGGATTTGAGGCGAAGGTAAGCTGGTAACTTCTCTGTCTATGAGAGCCGTTAAAACGACTCTCATAGATTTTATTAATAGCAAAATATAAACCGTCCCCAAAAAAGCCACCAACCACAAACCAGACAAATACCGAGCGAGAATACATATACCCAAAACTCGAACATTTCCCGCATAAAGAGTTTTCTTAAGATAAGAATAATAAGTGGC  >  NZ_CP025268/3174047‑3174318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`