BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,183,692	A→G	S153G (AGT→GGT)	zupT →	zinc transporter ZupT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,183,692	0	A	G	99.6%	817.3 / NA	250	S153G (AGT→GGT)	zupT	zinc transporter ZupT
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (122/127); minor base T (1/0); total (123/127)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 250 total aligned reads displayed.

AGCCATTCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGTAGCATGATTTCC  >  NZ_CP025268/3183559‑3183826

    aTTCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGACGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCACACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCtggcagt                                                                                                                              >  1:4111809/1‑140 (MQ=255)

      tCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCtggcagtgg                                                                                                                            <  2:4487194/140‑1 (MQ=255)

      tCTGCTCACTCTCGGCATCAGTCTGCATAAATTCCCGGAAGGGCTTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCtggcagtgg                                                                                                                            <  1:3831550/140‑1 (MQ=255)

        tGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCtggcagtggca                                                                                                                          <  1:3079907/140‑1 (MQ=255)

        tGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCtggcagtggca                                                                                                                          <  1:2129305/140‑1 (MQ=255)

          cTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAgg                                                                                                                        >  1:1234716/1‑140 (MQ=255)

          cTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAgg                                                                                                                        >  1:3656867/1‑140 (MQ=255)

          cTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAgg                                                                                                                        >  2:4925483/1‑140 (MQ=255)

          cTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAgg                                                                                                                        >  2:4843704/1‑140 (MQ=255)

          cTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAgg                                                                                                                        >  2:2231065/1‑140 (MQ=255)

          cTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAgg                                                                                                                        >  2:2451859/1‑140 (MQ=255)

           tCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGc                                                                                                                       <  2:5174883/140‑1 (MQ=255)

            cACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGcc                                                                                                                      >  2:545459/1‑140 (MQ=255)

            cACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGcc                                                                                                                      >  2:2577495/1‑140 (MQ=255)

             aCTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGccc                                                                                                                     >  1:1789968/1‑140 (MQ=255)

               tctcGGCATCAGTCTGCATACCTTCCCGGAAGGGCTTGCCACGTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCGTCTCACTGGCCGTCGCGTTGCACGATATGCCTGAAGGTCTTGCAGGGGCAGGTCCgg                                                                                                                   >  2:249048/1‑140 (MQ=255)

               tctcGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCtggcagtgg                                                                                                                            >  1:3954812/1‑131 (MQ=255)

               tctcGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCtggcagtgg                                                                                                                            <  2:3954812/131‑1 (MQ=255)

                  cGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGttt                                                                                                                >  2:5261527/1‑140 (MQ=255)

                  cGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGttt                                                                                                                >  2:940587/1‑140 (MQ=255)

                   ggCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTa                                                                                                               <  1:469920/140‑1 (MQ=255)

                         aGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGc                                                                                                         >  2:3117327/1‑140 (MQ=255)

                         aGTCTGCATAACTTCCCGGAAGGGATTGACACCATTGTCACGGCAAGCAACAACATGGAGCTGGGATTTCCCATCGCACTGGACGTCGCGTTGCACAATATCCCTAAAGGTCTGACAGTGTCAGGCCCGGATTATGCGGc                                                                                                         >  2:3734866/1‑140 (MQ=255)

                           tCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCaa                                                                                                       >  1:2636420/1‑140 (MQ=255)

                           tCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCaa                                                                                                       >  2:2286297/1‑140 (MQ=255)

                           tCTGCATAACTTCCCGGAAGGGAGTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCTCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCaa                                                                                                       >  1:4800209/1‑140 (MQ=255)

                            cTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAAc                                                                                                      <  2:3218545/140‑1 (MQ=255)

                            cTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAAc                                                                                                      <  1:340503/140‑1 (MQ=255)

                            cTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAAc                                                                                                      <  1:44740/140‑1 (MQ=255)

                             tGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACg                                                                                                     <  2:3307981/140‑1 (MQ=255)

                             tGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACg                                                                                                     <  2:1611292/140‑1 (MQ=255)

                              gCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACgg                                                                                                    <  1:4933803/140‑1 (MQ=255)

                              gCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACgg                                                                                                    <  1:1060791/140‑1 (MQ=255)

                              gCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACgg                                                                                                    <  1:280284/140‑1 (MQ=255)

                                ttAACTTCCTCGCAGCGATTGCCACTTTTGTCACGTCGAGCAGCCACCAGTAGATCTGCTTTGGCATCCCACTGGCGGTCGCGTTGCACACTATCCCGTACGGTCTGACCGTGGCAGGCCCGGTTTATGCGGCAAccgtg                                                                                                  <  2:2241489/139‑5 (MQ=255)

                                aTAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACgggg                                                                                                  <  2:1293084/140‑1 (MQ=255)

                                aTAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGGGGCAGGCCCGGTTTATGCGGCAACgggg                                                                                                  <  1:2025156/140‑1 (MQ=255)

                                    cTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCACCCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTa                                                                                              >  1:2370522/1‑140 (MQ=255)

                                    cTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTa                                                                                              >  1:4591127/1‑140 (MQ=255)

                                     ttCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTaa                                                                                             >  2:3468578/1‑140 (MQ=255)

                                     ttCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTaa                                                                                             >  1:2276707/1‑140 (MQ=255)

                                        ccGGAAGGGATTGCCACCTTTGTCGCGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCCGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACg                                                                                          <  2:314688/140‑1 (MQ=255)

                                        ccGGAAGGGATTGCCACCTTTGTCACGGCTAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACg                                                                                          <  2:5341841/140‑1 (MQ=255)

                                        ccGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACg                                                                                          <  2:3656867/140‑1 (MQ=255)

                                        ccGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACg                                                                                          <  1:3261354/140‑1 (MQ=255)

                                        ccGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACg                                                                                          <  2:577519/140‑1 (MQ=255)

                                        caggcaGGGATTCACACCTTTGTCAAGGCGAGCAGCACCCTGGCGCTGGGATTTGGCATCGCACTCGCAGTCGCGTTGCACAATCTCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACg                                                                                          <  2:4089744/135‑1 (MQ=255)

                                          ggAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCa                                                                                        <  2:1261252/140‑1 (MQ=255)

                                             aGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACcg                                                                                     <  2:48744/140‑1 (MQ=255)

                                             aGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACcg                                                                                     <  2:2780260/140‑1 (MQ=255)

                                             aGGGAGTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCTCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACcg                                                                                     <  2:1828837/140‑1 (MQ=255)

                                                gATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGGCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGGCTAAACGCACCGCGa                                                                                  <  1:1047685/140‑1 (MQ=255)

                                                 aTTGCCGCCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGAGTTGGCATCGCACTGGCCGTCGCGGCGCACAATTTCCCTGAAGGTCTGGCAGTGGCAGGACCGGTTTATGCGGCAACGGGGTCTGAACGCTCCGCGAt                                                                                 >  2:2851342/1‑140 (MQ=255)

                                                 aTTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGAt                                                                                 >  2:4999719/1‑140 (MQ=255)

                                                 aTTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCGGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGAt                                                                                 >  2:3254348/1‑140 (MQ=255)

                                                  ttGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGGCTAAACGCACCGCGAtt                                                                                >  1:3991900/1‑140 (MQ=255)

                                                   tGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTc                                                                               <  2:795740/140‑1 (MQ=255)

                                                   tGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTc                                                                               >  1:1593536/1‑140 (MQ=255)

                                                   tGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTc                                                                               <  2:4670565/140‑1 (MQ=255)

                                                    gCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCt                                                                              <  1:5296053/140‑1 (MQ=255)

                                                            tGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCgg                                                                      >  2:508000/1‑140 (MQ=255)

                                                            tGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGACTAAACGCACCGCGATTCTGTGGGCgg                                                                      >  2:5209534/1‑140 (MQ=255)

                                                             gTCACGGCGAGCAGCAACCTCGAGCTTGGCTTTGGCATCGCTCTGGCCGTCGCGTTCCACGATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCggg                                                                     <  2:1606085/140‑1 (MQ=255)

                                                                      agcagcAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGAGTTCTgg                                                            >  1:4433011/1‑140 (MQ=255)

                                                                       gcagcaACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGa                                                           <  1:1978475/140‑1 (MQ=255)

                                                                         agcaACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACt                                                         <  1:3556636/140‑1 (MQ=255)

                                                                                tGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGaa                                                  <  1:3903023/140‑1 (MQ=255)

                                                                                tGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGaa                                                  <  1:162247/140‑1 (MQ=255)

                                                                                tGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGaa                                                  <  1:3252151/140‑1 (MQ=255)

                                                                                     cTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAGCGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCt                                             <  2:1797210/140‑1 (MQ=255)

                                                                                        ggATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTtgg                                          >  1:3581588/1‑140 (MQ=255)

                                                                                          aTTTGGCATCGCACTGGCCGTCGCGTTGCACACTATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTTGACTAGCAGAAATTCTtggtg                                        <  2:4445097/140‑1 (MQ=255)

                                                                                           tttGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTtggtgg                                       <  1:2380024/140‑1 (MQ=255)

                                                                                                   cGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTTGTGTGCTgg                               >  2:1999440/1‑140 (MQ=255)

                                                                                                   cGCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTgg                               >  1:1733849/1‑140 (MQ=255)

                                                                                                   cGCACTGGCCGTCGCGCTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTCTGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTgg                               <  1:4273800/140‑1 (MQ=255)

                                                                                                    gCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGc                              <  1:1194983/140‑1 (MQ=255)

                                                                                                    gCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGc                              <  1:1961760/140‑1 (MQ=255)

                                                                                                    gCACTGGCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCACGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGCGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGc                              <  1:303160/140‑1 (MQ=255)

                                                                                                         ggcgctggcgTTGCACACTATCCCTGCAGGTCTGGCAGTGGCCGGCCCGGTTTATGGCGCACCGGGGTCTAAAGGCACCGGGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGt                         <  2:1038578/133‑1 (MQ=255)

                                                                                                         ggCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCCGGCCCGGTTTATGCGGCAACGGCGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGt                         <  2:290108/140‑1 (MQ=255)

                                                                                                         ggCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTCTGCGGCAACGGGGTCTAAACGCACCGCGATGCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGt                         <  2:2130534/140‑1 (MQ=255)

                                                                                                         ggCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGt                         >  1:747412/1‑140 (MQ=255)

                                                                                                         ggCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGt                         <  1:4681391/140‑1 (MQ=255)

                                                                                                          gCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTTTGCGGCAACGGGGGCTAAACGCACCGCGATTCTGTGGGCGGGGGTTTCTGGACTAGCAGAAATTTTTGGTGGTGTGCTGGCGTGGtt                        >  1:5170640/1‑140 (MQ=255)

                                                                                                          gCCGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGtt                        >  1:2935451/1‑140 (MQ=255)

                                                                                                           ccGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTa                       <  1:2256071/140‑1 (MQ=255)

                                                                                                           ccGTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTa                       <  2:1298066/140‑1 (MQ=255)

                                                                                                             gTCGCGTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTAGGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGTGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTGAAt                     >  2:3786175/1‑140 (MQ=255)

                                                                                                               cgcgTTGCACAATATCCCTGACGGTCTGGCATTGGCCGGCCCGGTTTATGCGCCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATcc                   >  2:878531/1‑140 (MQ=255)

                                                                                                               cgcgTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATcc                   >  1:950045/1‑140 (MQ=255)

                                                                                                               cgcgTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGGCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATcc                   >  1:1567726/1‑140 (MQ=255)

                                                                                                                gcgTTGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGGGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCt                  <  2:3569530/140‑1 (MQ=255)

                                                                                                                    tGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGt              >  1:917089/1‑140 (MQ=255)

                                                                                                                    tGCACAATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGt              >  1:4877149/1‑140 (MQ=255)

                                                                                                                      cacaATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGTAg            <  1:3343360/140‑1 (MQ=255)

                                                                                                                      cacaATATCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGTAg            <  1:2741775/140‑1 (MQ=255)

                                                                                                                          atatCCCTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGTAGCATg        <  2:2746044/140‑1 (MQ=255)

                                                                                                                              cccTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCGGGCGTGGTTAATCCTCGGTAGCATGAttt    >  1:1409725/1‑140 (MQ=255)

                                                                                                                                cTGAAGGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGTAGCATGATTTcc  >  2:4579059/1‑140 (MQ=255)

AGCCATTCTGCTCACTCTCGGCATCAGTCTGCATAACTTCCCGGAAGGGATTGCCACCTTTGTCACGGCGAGCAGCAACCTGGAGCTGGGATTTGGCATCGCACTGGCCGTCGCGTTGCACAATATCCCTGAAAGTCTGGCAGTGGCAGGCCCGGTTTATGCGGCAACGGGGTCTAAACGCACCGCGATTCTGTGGGCGGGGATTTCTGGACTAGCAGAAATTCTTGGTGGTGTGCTGGCGTGGTTAATCCTCGGTAGCATGATTTCC  >  NZ_CP025268/3183559‑3183826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`