BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,232,860	T→C	N170N (AAT→AAC)	fadH →	NADPH‑dependent 2,4‑dienoyl‑CoA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,232,860	0	T	C	100.0%	1328.2 / NA	390	N170N (AAT→AAC)	fadH	NADPH‑dependent 2,4‑dienoyl‑CoA reductase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (186/204); total (186/204)

Only 100 of 390 total aligned reads displayed.

AACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGA  >  NZ_CP025268/3232723‑3232995

aaCCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGa                                                                                                                                       <  1:903656/140‑1 (MQ=255)

aaCCGGTTCGGGCCCCATGAGTTAAGCCATGAAGAGATCATGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGGGTAGAGGTGATGGGTTCCGAAGGGTATTTGAGCAACGa                                                                                                                                       <  2:1085102/140‑1 (MQ=255)

     tttCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTc                                                                                                                                  >  1:5120032/1‑140 (MQ=255)

     tttCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGAGCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGGGATGGGTTCCGAAGGGTATTTGATCAACGAATTTc                                                                                                                                  >  1:292486/1‑140 (MQ=255)

        cGTGCCCCATGAGTTAAGCCATGAAGAGCTCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGa                                                                                                                               <  1:1110576/140‑1 (MQ=255)

        cGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGGGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGa                                                                                                                               <  2:952781/140‑1 (MQ=255)

          tGCCCCATGAGTTGCGCCATGAAGAGCTCCTGAAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGCTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACg                                                                                                                             <  2:3255201/140‑1 (MQ=255)

          tGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGGCg                                                                                                                             >  2:4228690/1‑140 (MQ=255)

          tGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACg                                                                                                                             >  1:146759/1‑140 (MQ=255)

          tGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACg                                                                                                                             >  2:3152566/1‑140 (MQ=255)

            ccccATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCt                                                                                                                           <  2:2286715/140‑1 (MQ=255)

            ccccATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCt                                                                                                                           <  1:2674950/140‑1 (MQ=255)

            ccccATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAACTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGCGACGGGTTCCGCAGGGGATTTGATCAACGAATTTCTGACGCt                                                                                                                           >  2:1464062/1‑140 (MQ=255)

                 tGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCa                                                                                                                      <  1:374985/140‑1 (MQ=255)

                 tGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCa                                                                                                                      <  2:3530857/140‑1 (MQ=255)

                   aGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCAcc                                                                                                                    <  1:3953224/140‑1 (MQ=255)

                   aGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCAcc                                                                                                                    <  1:66940/140‑1 (MQ=255)

                      tAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAAt                                                                                                                 <  2:4162469/140‑1 (MQ=255)

                      tAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAAt                                                                                                                 <  2:3623685/140‑1 (MQ=255)

                        aGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCACGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTTATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCa                                                                                                               <  2:1132446/140‑1 (MQ=255)

                         gCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCGCCAATCAg                                                                                                              >  2:1980591/1‑140 (MQ=255)

                           cATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCg                                                                                                            <  2:208755/140‑1 (MQ=255)

                            aTGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGt                                                                                                           >  2:2393084/1‑140 (MQ=255)

                             tGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTa                                                                                                          <  2:1513079/140‑1 (MQ=255)

                             tGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTa                                                                                                          >  1:1796196/1‑140 (MQ=255)

                             tGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTa                                                                                                          >  2:2986176/1‑140 (MQ=255)

                                  agaTCCTGCAACTGATCGACAATTTCTCCCGCTGCGCGCAACTAGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTCCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGAc                                                                                                     <  2:4376607/140‑1 (MQ=255)

                                    aTCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCa                                                                                                   >  2:1642504/1‑140 (MQ=255)

                                    aTCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCa                                                                                                   >  1:368463/1‑140 (MQ=255)

                                    aTCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGCTCGGGGGTTGCGGAGGCAATTAAATAAACGAAGTTCTGACGCTGCGCACCACTCATCGTAGTGACCa                                                                                                   >  2:671055/1‑140 (MQ=255)

                                     tCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAg                                                                                                  <  1:2276870/140‑1 (MQ=255)

                                      ccTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGt                                                                                                 >  2:1370131/1‑140 (MQ=255)

                                         gCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTggg                                                                                              >  1:1696545/1‑140 (MQ=255)

                                         gCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTggg                                                                                              <  2:519720/140‑1 (MQ=255)

                                            aCTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGggcg                                                                                           <  1:3885581/140‑1 (MQ=255)

                                              tGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGggcggc                                                                                         >  2:5356071/1‑140 (MQ=255)

                                                aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGTTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACTAGTGGGGCAGCGa                                                                                       >  2:4724244/1‑140 (MQ=255)

                                                aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCACTCAGCGTAGTGACCAGTGGGGCGGCGa                                                                                       >  2:1036437/1‑140 (MQ=255)

                                                aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGa                                                                                       >  1:944903/1‑140 (MQ=255)

                                                aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGa                                                                                       >  2:4491388/1‑140 (MQ=255)

                                                aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGa                                                                                       >  2:2596866/1‑140 (MQ=255)

                                                aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGa                                                                                       >  1:501539/1‑140 (MQ=255)

                                                aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGa                                                                                       >  1:1871807/1‑140 (MQ=255)

                                                aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGa                                                                                       >  1:4047466/1‑140 (MQ=255)

                                                aTCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTAATTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTCACCAGTGGGGCGGCGa                                                                                       >  1:2951087/1‑140 (MQ=255)

                                                 tCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGAt                                                                                      <  1:1340774/140‑1 (MQ=255)

                                                    aCAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTAc                                                                                   >  1:3576150/1‑140 (MQ=255)

                                                             cccGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCgg                                                                          >  1:1965556/1‑140 (MQ=255)

                                                             cccGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCgg                                                                          >  1:1002891/1‑140 (MQ=255)

                                                                gCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATg                                                                       <  1:2456608/140‑1 (MQ=255)

                                                                    cgcgcAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGAt                                                                   >  2:4250982/1‑140 (MQ=255)

                                                                     gcgcAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGAtt                                                                  <  2:5120032/140‑1 (MQ=255)

                                                                     gcgcAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGAtt                                                                  <  1:2103102/140‑1 (MQ=255)

                                                                        cAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCACTCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGc                                                               <  2:4807160/140‑1 (MQ=255)

                                                                        cAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCCGCGTAGTGACCAGTGGGGCGGCGATTCCCGCAACCGGATGCGATTTGc                                                               <  2:4968649/140‑1 (MQ=255)

                                                                        cAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGc                                                               <  2:4246221/140‑1 (MQ=255)

                                                                        cAACTGGCGCGGGAGGCAGGATACGACGGAGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGc                                                               <  2:3749280/140‑1 (MQ=255)

                                                                          aCTGGCTCAGGAGGCAGGATAAGACGGTGTAGAGGTGATGAGTTCCGAATGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGCCCAGTGGGGCGGCTATTACCGCAACCGGATGCGATTTGCCg                                                             <  2:2872309/140‑1 (MQ=255)

                                                                          aCTGGCGCGGGAGGCAGGATGCGAGGGTGTAGAGGTCATGTGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCg                                                             <  2:1227906/140‑1 (MQ=255)

                                                                          aCTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCg                                                             <  1:2990830/140‑1 (MQ=255)

                                                                          aCTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCg                                                             <  1:1217940/140‑1 (MQ=255)

                                                                          aCTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCg                                                             <  2:5328921/140‑1 (MQ=255)

                                                                          aCTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCg                                                             <  1:3175524/140‑1 (MQ=255)

                                                                           cTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGt                                                            >  2:4001427/1‑140 (MQ=255)

                                                                            tGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTa                                                           <  2:4537078/140‑1 (MQ=255)

                                                                                 cgGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAagt                                                      <  1:3333911/140‑1 (MQ=255)

                                                                                   ggAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGGGGCGGACCAATCAGCGTAGTGACTAGTGGGGTGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAagtag                                                    <  2:4871953/140‑1 (MQ=255)

                                                                                   ggAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAagtag                                                    >  2:4996111/1‑140 (MQ=255)

                                                                                   ggAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAagtag                                                    <  1:435237/140‑1 (MQ=255)

                                                                                         aGGATACGACGGTGTAGAGGTGATGGGTTCCGCAGGGTATTTGATCAACGAATTGCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAgtgcgtg                                              <  1:2512020/140‑1 (MQ=255)

                                                                                             tACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCgtgcggt                                          <  2:2925400/140‑1 (MQ=255)

                                                                                                  cgGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATGCCCGAATTTCGGACGCTGCGTAACAAGCAGCGGGCTGACCCGGGGGTCGGCGATTGCCGGTGCCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGcgcg                                     <  2:3864921/140‑1 (MQ=255)

                                                                                                  cgGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGcgcg                                     >  1:4526441/1‑140 (MQ=255)

                                                                                                     tgtAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCa                                                                                                                      >  1:1054406/1‑56 (MQ=255)

                                                                                                     tgtAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGaat                                  >  1:4712292/1‑139 (MQ=255)

                                                                                                     tgtAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAAc                                  >  2:2767536/1‑140 (MQ=255)

                                                                                                     tgtAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAAc                                  >  2:1810486/1‑140 (MQ=255)

                                                                                                     tgtAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAAc                                  >  1:2850213/1‑140 (MQ=255)

                                                                                                     tgtAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAAc                                  >  1:1254293/1‑140 (MQ=255)

                                                                                                     tgtAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAAc                                  >  2:2493985/1‑140 (MQ=255)

                                                                                                     tgtAGAGGTGATGGGTTCCGAAGGGTATTGGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAAc                                  >  1:1416718/1‑140 (MQ=255)

                                                                                                       tAGAGGTGATGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAAcgc                                >  2:4132050/1‑140 (MQ=255)

                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGGACGAATCAGCGTAGTGACCAGTGGGGGGGCGCTGACTGCAACCGGATGCGATTTGACGTAGAAGTAGTGCGTGCGGTGCGCGAAAGCGTCGGCaa                        >  2:2584635/1‑140 (MQ=255)

                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                        >  1:3418236/1‑140 (MQ=255)

                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                        >  1:418947/1‑140 (MQ=255)

                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                        >  1:4493500/1‑140 (MQ=255)

                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                        <  1:4941700/140‑1 (MQ=255)

                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                        >  2:4669311/1‑140 (MQ=255)

                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                        >  2:2146453/1‑140 (MQ=255)

                                                                                                               aTGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCaa                        >  2:4445684/1‑140 (MQ=255)

                                                                                                                tGGGTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAAc                       <  2:3332994/140‑1 (MQ=255)

                                                                                                                  ggTTCCGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGa                     <  2:86288/140‑1 (MQ=255)

                                                                                                                   gTTCCGAAGGGTATTTGATCAACGCATTTCTGACGCTGCGCCCCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGAc                    <  1:2724111/140‑1 (MQ=255)

                                                                                                                       cGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCa                <  2:1396598/140‑1 (MQ=255)

                                                                                                                       cGAAGGGTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCa                <  2:1072781/140‑1 (MQ=255)

                                                                                                                           gggTATTTGATCAACGAATTTCTGACGCTGCGCACCAATAAGCGCAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTGGCCTTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCattat            <  1:5078509/140‑1 (MQ=255)

                                                                                                                            ggTATTTGATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCattatt           >  2:5262606/1‑140 (MQ=255)

                                                                                                                                   gATCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTAcc    >  2:4035563/1‑140 (MQ=255)

                                                                                                                                     tCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTCCCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGa  <  1:3772460/140‑1 (MQ=255)

                                                                                                                                     tCAACGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGa  <  1:2568039/140‑1 (MQ=255)

AACCGTTTCGTGCCCCATGAGTTAAGCCATGAAGAGATCCTGCAACTGATCGACAATTTCGCCCGCTGCGCGCAACTGGCGCGGGAGGCAGGATACGACGGTGTAGAGGTGATGGGTTCCGAAGGGTATTTGATCAATGAATTTCTGACGCTGCGCACCAATCAGCGTAGTGACCAGTGGGGCGGCGATTACCGCAACCGGATGCGATTTGCCGTAGAAGTAGTGCGTGCGGTGCGCGAACGCGTCGGCAACGACTTCATTATTATCTACCGA  >  NZ_CP025268/3232723‑3232995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`