Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,266,812	T→C	*313* (TAA→TAG)	tdcA ←	transcriptional regulator TdcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,266,812	0	T	C	100.0%	285.0 / NA	85	*313* (TAA→TAG)	tdcA	transcriptional regulator TdcA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (51/34);  total (51/34)

TCATCAATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAATTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACA  >  NZ_CP025268/3266676‑3266944                                                                                                                                         |                                                                                                                                     tcatcaATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTaa                                                                                                                                   >  1:1162386/1‑140 (MQ=255)   atcaATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAAcc                                                                                                                                 >  1:5292777/1‑140 (MQ=255)   atcaATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAAcc                                                                                                                                 >  1:3012490/1‑140 (MQ=255)   atcaATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAAcc                                                                                                                                 >  2:5208419/1‑140 (MQ=255)    tcaATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCa                                                                                                                                >  2:314819/1‑140 (MQ=255)      aaTAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAAc                                                                                                                              <  1:1401929/140‑1 (MQ=255)      aaTAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAAc                                                                                                                              <  1:546436/140‑1 (MQ=255)         aGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTc                                                                                                                           <  1:2349100/140‑1 (MQ=255)           cAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTa                                                                                                                         >  2:952572/1‑140 (MQ=255)             aCCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTAtt                                                                                                                       >  1:885445/1‑140 (MQ=255)                  cAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTg                                                                                                                  >  1:4683854/1‑140 (MQ=255)                    gATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGcc                                                                                                                <  2:4577255/140‑1 (MQ=255)                     aTCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCt                                                                                                               <  1:1969899/140‑1 (MQ=255)                      tCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATATCTAACCAACTTCTATTAATTGCCtt                                                                                                              <  2:2648034/140‑1 (MQ=255)                      tCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCtt                                                                                                              <  2:2911197/140‑1 (MQ=255)                      tCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCtt                                                                                                              <  1:3469444/140‑1 (MQ=255)                           tGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACTGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACt                                                                                                                             >  2:4194246/1‑120 (MQ=255)                           tGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACt                                                                                                                             <  1:4194246/120‑1 (MQ=255)                           tGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCt                                                                                                         <  2:5076771/140‑1 (MQ=255)                            gTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTa                                                                                                        <  1:4313573/140‑1 (MQ=255)                               atatGCATTATAAAATCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAATACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTacac                                                                                                     >  1:2157905/1‑140 (MQ=255)                               atatGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTacac                                                                                                     <  1:4338229/140‑1 (MQ=255)                               atatGCATTATAAAACCTCTTCACCTCAATTATATATGTAGTCTACCGTAACCGACACCTCCAAGATCGACACGTATATTACTTCCGTCTAAATAAAAACAACTAACTAACCAACTTCTCTGAATTGCCCTCCTCTacac                                                                                                     <  2:1391346/140‑1 (MQ=255)                                tatGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACAcc                                                                                                    >  2:4980225/1‑140 (MQ=255)                                tatGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACAcc                                                                                                    >  1:1889879/1‑140 (MQ=255)                                tatGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACAcc                                                                                                    <  1:3506064/140‑1 (MQ=255)                                tatGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACAcc                                                                                                    >  2:4462888/1‑140 (MQ=255)                                tatGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACAcc                                                                                                    >  2:5248794/1‑140 (MQ=255)                                    cATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCAtt                                                                                                >  1:1814979/1‑140 (MQ=255)                                     aTTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCAtga                                                                                               >  2:557632/1‑138 (MQ=255)                                     aTTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATta                                                                                               >  2:557401/1‑140 (MQ=255)                                     aTTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATta                                                                                               >  2:3168647/1‑140 (MQ=255)                                     aTTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATta                                                                                               >  1:4970480/1‑140 (MQ=255)                                     aTTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATta                                                                                               >  2:2470996/1‑140 (MQ=255)                                      ttATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATtat                                                                                              <  2:3580147/140‑1 (MQ=255)                                      ttATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACAAACTTCTATTAATTGCCTTCGTCTACACCCATtat                                                                                              <  2:3580313/140‑1 (MQ=255)                                       tataAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATtata                                                                                             <  1:1576971/140‑1 (MQ=255)                                       tataAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATtata                                                                                             >  2:2480105/1‑140 (MQ=255)                                              ccTCTTCGCCTGAATTAAATATGTAGGTAACCGTAAGCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGaa                                                                                      <  1:5278658/140‑1 (MQ=255)                                              ccTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGaa                                                                                      >  2:1013982/1‑140 (MQ=255)                                              ccTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGCATTATTTATGTTTAAATAACAACAAATACCTAACCAACTTCTATCAATTGCCTTCGTCTACACCCATTATAAGCTGaa                                                                                      <  2:2156278/140‑1 (MQ=255)                                                 ctTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATaa                                                                                            <  1:4114401/131‑1 (MQ=255)                                                 ctTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATaa                                                                                            <  1:4114380/131‑1 (MQ=255)                                                 ctTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATaa                                                                                            >  2:4114380/1‑131 (MQ=255)                                                 ctTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATaa                                                                                            >  2:4114401/1‑131 (MQ=255)                                                 ctTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATAc                                                                                   >  1:3898432/1‑140 (MQ=255)                                                 ctTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATAc                                                                                   >  1:232524/1‑140 (MQ=255)                                                 ctTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATAc                                                                                   >  1:1113119/1‑140 (MQ=255)                                                 ctTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACAACTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATAc                                                                                   >  1:105359/1‑140 (MQ=255)                                                   tCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGAGTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATActc                                                                                 >  2:725471/1‑140 (MQ=255)                                                    cGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATActct                                                                                <  1:314819/140‑1 (MQ=255)                                                    cGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATActct                                                                                <  1:1485347/140‑1 (MQ=255)                                                          aaTTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCt                                                                          <  1:5175226/140‑1 (MQ=255)                                                                    gTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTccacca                                                                >  1:3169903/1‑140 (MQ=255)                                                                    gTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGGTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTTCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTccacca                                                                >  2:2768648/1‑140 (MQ=255)                                                                       ggTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCaaaa                                                             >  2:3044583/1‑140 (MQ=255)                                                                         taaccgtaaccgACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTacac                                                                                                     >  2:878370/1‑98 (MQ=255)                                                                         taaccgtaaccgACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTacac                                                                                                     <  1:878370/98‑1 (MQ=255)                                                                           accgtaaccgACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTACCCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGa                                                         >  1:3856173/1‑140 (MQ=255)                                                                           accgtaaccgACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGa                                                         >  1:2908639/1‑140 (MQ=255)                                                                             cgttaccgACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTCACCACCTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGATAATTCCACCAAAACCGAtg                                                       <  2:3711139/140‑1 (MQ=255)                                                                               taaccgACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGAtgct                                                     >  2:1479616/1‑140 (MQ=255)                                                                               taaccgACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGAtgct                                                     >  1:1496376/1‑140 (MQ=255)                                                                               taaccgACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGAtgct                                                     >  2:1781647/1‑140 (MQ=255)                                                                                           gCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATa                                         >  1:1834485/1‑140 (MQ=255)                                                                                             aaGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACg                                       >  1:962429/1‑140 (MQ=255)                                                                                               gacagacaGGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGAt                                     >  2:5239688/1‑140 (MQ=255)                                                                                                    acagGTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAAttt                                >  1:1032233/1‑140 (MQ=255)                                                                                                      agGTGGATCATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGACTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGTTGCTGCTTTTTTAATACGATAAttttt                              <  2:3928521/140‑1 (MQ=255)                                                                                                       cgTGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACGCCCATTATAAGATGAATACTCTTTGGCTAATTGCACCAAAGCCGTTGCTGCTTTTTCAATACGATAATTTTTc                             >  2:4425863/2‑140 (MQ=255)                                                                                                         tGGATTATTTATGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGa                           >  1:3647631/1‑140 (MQ=255)                                                                                                              tatttatGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATa                      <  2:3187890/140‑1 (MQ=255)                                                                                                              tatttatGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATAAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATa                      <  2:1814979/140‑1 (MQ=255)                                                                                                                  tatGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTa                                                                                                        >  2:3831095/1‑54 (MQ=255)                                                                                                                  tatGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTa                                                                                                        >  2:3830516/1‑54 (MQ=255)                                                                                                                  tatGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTa                                                                                                        <  1:3830516/54‑1 (MQ=255)                                                                                                                  tatGTTTAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTa                                                                                                        <  1:3831095/54‑1 (MQ=255)                                                                                                                      tttAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCa              >  1:2423442/1‑140 (MQ=255)                                                                                                                      tttAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCa              >  1:439163/1‑140 (MQ=255)                                                                                                                      tttAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCa              >  2:3113381/1‑140 (MQ=255)                                                                                                                      tttAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCa              >  1:5350131/1‑140 (MQ=255)                                                                                                                      tttAAATAAAAACAAATAACTAACCAACTTCTATTAATTGCCTTCATCTACACCCATTATAAGATGAATACTCTTTGGCTAATTTCACCAAAACCGATGCTGCCTTTTTAATACGATAATTTTTCGACCATACCGCGGCa              <  2:4620409/140‑1 (MQ=255)                                                                                                                              aaaaCAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGAGGCATATTGTGc      >  1:375627/1‑140 (MQ=255)                                                                                                                                aaCAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCca    <  2:4970480/140‑1 (MQ=255)                                                                                                                                  cAAATAACTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCcaca  <  1:4833186/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     TCATCAATAGCAACCGGCAGATCGTATGTAATATGCATTATAAAACCTCTTCGCCTGAATTAAATATGTAGGTAACCGTAACCGACACCTGCAAGACAGACAGGTGGATTATTTATGTTTAAATAAAAACAAATAATTAACCAACTTCTATTAATTGCCTTCGTCTACACCCATTATAAGATGAATACTCTTTGGCTAATTCCACCAAAACCGATGCTGCTTTTTTAATACGATAATTTTTCGACCATACCGCGGCATATTGTGCCACA  >  NZ_CP025268/3266676‑3266944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑