BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,458,302	A→G	T414A (ACC→GCC)	gspD →	type II secretion system secretin GspD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,458,302	0	A	G	100.0%	937.4 / NA	284	T414A (ACC→GCC)	gspD	type II secretion system secretin GspD
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (132/152); total (132/152)

Only 100 of 284 total aligned reads displayed.

GGGCGAATAAAAACGTTGGCGCACAGCAATTTACCAATACCGGATTACCGATTTTTAACGCTGCGCAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCACCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTCGCGTCCTTCAA  >  NZ_CP025268/3458168‑3458438

gggCGAATAAAAACGTTGGCGCACAGCAATTTACCAATACCGGACTACCGATTTTTAACGCTGCGCAAGGCGGGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTGAGCGCCTAc                                                                                                                                     >  2:3935241/1‑140 (MQ=255)

     aaTAAAAACGTTGGCGCACAGCAATTTACCAATACCGGATTACCGCTTTTTAACGCTGCGCAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAatgg                                                                                                                                >  1:1528963/1‑140 (MQ=255)

      aTAAAAACGTTGGCGCACAGCAATTTACCAATACCGGATTACCGATTTTTAACGCTGCGCAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAatggc                                                                                                                               <  2:462805/140‑1 (MQ=255)

       tAAAAACGTTGGCGCACAGCAATTGCCCAATACCGGATTACCGATTTTTAACGCTGCGCAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAatggca                                                                                                                              <  2:4228945/140‑1 (MQ=255)

        aaaaaCGTTGGCGCACAGCAAGTTGTCTATACCGGATTACCAATTTTTAACGCTGCCCAAGGTGTTGCTGATAATAAAAAGAATTGTGGGATCACCAGCCCGAATCCTCCCTGAGACATGTTTAGCGCGTACAatggcat                                                                                                                             <  2:5326307/140‑1 (MQ=255)

             cGTTGGCGCACAGCAATTTACCAATACCGGATTACCGATTTTTAACGCTGCGCAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCg                                                                                                                        <  1:1859062/140‑1 (MQ=255)

             cGTTGGCGCACAGCAATTTACCAATACCGGATTACCGATTTTTAACGCTGCGCAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCg                                                                                                                        <  1:988978/140‑1 (MQ=255)

               ttGGCGCACAGCAATTTACCAATACCGGATTACCGATTTTTAACGCTGCGCAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCa                                                                                                                      <  1:4315792/140‑1 (MQ=255)

                tGGCGCACAGCAATTTACCAATACCGGATTACCGATTTTTAACGCTGCGCAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAg                                                                                                                     >  1:3944153/1‑140 (MQ=255)

                tGGCGCACAGCAATTTACCAATACCGGATTACCGATTTTTAACGCTGCGCAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAg                                                                                                                     <  2:4402619/140‑1 (MQ=255)

                 ggCGCACAGCAATTTACCAATACCGGATTACCGATTTTTAACGCTGCGCAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTGCAATGGCATGGCCGCAgg                                                                                                                    >  2:3808847/1‑140 (MQ=255)

                  gcgcACAGCAATTTACCAATACCGGATTACCGATTTTTAACGCTGCGCAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGc                                                                                                                   <  1:1114319/140‑1 (MQ=255)

                  gcgcACAGCAATTTACCAATACCGGATTACCGATTTTTAACGCTGCGCAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGc                                                                                                                   <  2:3417358/140‑1 (MQ=255)

                  gcgcACAGCAATTTACCAATACCGGATTACCGATTTTTAACGCTGCGCAAGGTGTGGCTGATTATAAAAAGAATGGGGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGc                                                                                                                   <  1:3925301/140‑1 (MQ=255)

                  gcgcACAGCAATTTACCAATACCGGATTACCGATTTTTAACGCTGAGCAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGc                                                                                                                   <  1:2671817/140‑1 (MQ=255)

                  gcgaACAGCAATGTAGCAATACCCGATTACCGCTTTTTAACGCTGCGCAAGGTGTGGCTGATTATACCAAGAATAGTGGCATAACCAGCGCGCATCCTAACTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGc                                                                                                                   <  2:2043333/140‑1 (MQ=255)

                           aaTTTACCAATACTGGATTACCGATTTTTAACGCTGCGCAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAAt                                                                                                          <  1:4706341/140‑1 (MQ=255)

                           aaTTTACCAATACCGGATTACCGATTTTTAACGCTGCGCAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAAt                                                                                                          <  2:1305109/140‑1 (MQ=255)

                            aTTTACCAATACCGGATTACCGATTTTTAACGCTGCGCAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATg                                                                                                         <  2:969934/140‑1 (MQ=255)

                              ttACCAATACCGGATTACCGATTTTTAACGCTGCGCACGGTGTCGCTGATCATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGc                                                                                                       <  2:1415831/140‑1 (MQ=255)

                               tACCAATACCGGATTACCGATTTTTAACGCTGCGCAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCg                                                                                                      >  1:3101096/1‑140 (MQ=255)

                                aCCAATACCGGATTACCGATTTTTAACGCTGCGCAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGa                                                                                                     <  1:1000894/140‑1 (MQ=255)

                                aCCAATACCGGATTACCGATTTTTAACGCTGCGCAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGa                                                                                                     <  2:2125279/140‑1 (MQ=255)

                                aCCAATACCGGATTACCGATTTTTAACGCTGCGCAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGa                                                                                                     <  2:4383697/140‑1 (MQ=255)

                                  cAATACCGGATTACCGATTTTTAACGCTGCGCAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACt                                                                                                   <  1:1288261/140‑1 (MQ=255)

                                   aaTACCGGATTACCGATTTTTAACGCTGCGCAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTg                                                                                                  <  2:136161/140‑1 (MQ=255)

                                    aTACCGGATTACCGATTTTTAACGCTGCTCCAGATGTAGATGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTACCTGGGATATGTTTCGGGCCGACAATGACATGGCCGCAGGATTCTTCAATGGCGACTgg                                                                                                 >  2:752380/1‑140 (MQ=255)

                                    aTACCGGATTACCGATTGTTAACGCTGCGCAAGGTGTGGCTGATCATAAAAAGAATGGTGGGTTCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCCGGCTTCTTCAATGGCGACTgg                                                                                                 >  2:2403022/1‑140 (MQ=255)

                                     tACCGGATTGCCGATTTTTAACGCTGCGCAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTggg                                                                                                <  1:2136059/140‑1 (MQ=255)

                                     tACCGGATTACCGATTTTTAACGCTGCGCAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCCCCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTggg                                                                                                <  1:3687885/140‑1 (MQ=255)

                                     tACCGGATTACCGATTTTTAACGCTGCGCAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTggg                                                                                                <  1:4550085/140‑1 (MQ=255)

                                       ccGGATTACCGATTTTTAACGCTGCGCAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTGCAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGa                                                                                              <  1:3604765/140‑1 (MQ=255)

                                       ccGGATTACCGATTTTTAACGCTGCGCAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGa                                                                                              <  1:5318785/140‑1 (MQ=255)

                                          gATTACCGATTTTTAACGCTGCGCAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTa                                                                                           >  2:830336/1‑140 (MQ=255)

                                               ccGATTTTTAACGCTGCGCAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCt                                                                                      >  1:3559403/1‑140 (MQ=255)

                                               ccGATTTTTAACGCTGCGCAAGGTGTGGCGGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCt                                                                                      >  1:2844508/1‑140 (MQ=255)

                                                cGATTTTTAACGCTGCGCAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCtt                                                                                     <  2:1767602/140‑1 (MQ=255)

                                                    ttttAACGCTGCGCAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACcg                                                                                 >  1:3405950/1‑140 (MQ=255)

                                                      ttAACGCTGTGCAAGGGGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGCGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATTTCCACTGGGGAGTACTGCTTACcgcg                                                                               <  2:3962110/140‑1 (MQ=255)

                                                             tGCGCAAGGGGTGGCTGATTATAAAAAGAATGGTGGGATCACGAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCa                                                                        >  1:4145799/1‑140 (MQ=255)

                                                              gcgcAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCGTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGTAGTACTGCTTACCGCGCTGACCAg                                                                       >  2:923250/1‑140 (MQ=255)

                                                              gcgcAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCTCTGGCCAg                                                                       >  2:2431644/1‑140 (MQ=255)

                                                              gcgcAAGGTGTAGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAGTAGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAg                                                                       >  1:4439183/1‑140 (MQ=255)

                                                               cgcAGGGTGGGGCTGGTTATAAAAAGAATGGTGGGGTCACCAGCGCGAGGCCTGGCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTAACGCGCTGGCGAgg                                                                      >  2:2529733/1‑139 (MQ=255)

                                                               cgcAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACGATGGCGTGGCCGCAGGCTTCTTCATTGGCGACTGGGGAGTACTGCGTACCGCGCTTGccggg                                                                      >  1:2795434/1‑137 (MQ=255)

                                                               cgcAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGt                                                                      <  1:573367/140‑1 (MQ=255)

                                                               cgcAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGt                                                                      <  1:4121855/140‑1 (MQ=255)

                                                                    ggTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACaa                                                                 >  1:1618909/1‑140 (MQ=255)

                                                                     gtgtGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAAt                                                                <  1:1174913/140‑1 (MQ=255)

                                                                       gtgGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATaa                                                              >  2:3099459/1‑140 (MQ=255)

                                                                         ggCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATaaaa                                                            >  1:5194445/1‑140 (MQ=255)

                                                                         ggCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATaaaa                                                            >  1:4144113/1‑140 (MQ=255)

                                                                         ggCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATaaaa                                                            >  2:3204363/1‑140 (MQ=255)

                                                                         ggCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGACATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATaaaa                                                            >  2:3564067/1‑140 (MQ=255)

                                                                                tataAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACAt                                                     >  2:1884228/1‑140 (MQ=255)

                                                                                tataAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACAt                                                     >  1:1654028/1‑140 (MQ=255)

                                                                                tataAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACAt                                                     >  2:2922576/1‑140 (MQ=255)

                                                                                tataAAAAGAATGGGGGGATCACCAGCGCGAATCCTGCCGGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACAt                                                     >  1:20957/1‑140 (MQ=255)

                                                                                     aaaGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCg                                                <  2:2702146/140‑1 (MQ=255)

                                                                                     aaaGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCg                                                <  2:3874527/140‑1 (MQ=255)

                                                                                     aaaGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCg                                                >  2:4116712/1‑140 (MQ=255)

                                                                                     aaaGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCg                                                >  1:4224817/1‑140 (MQ=255)

                                                                                       aGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGcc                                              <  2:1528963/140‑1 (MQ=255)

                                                                                         aaTGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCAc                                            >  1:2711225/1‑140 (MQ=255)

                                                                                            ggtggAATCACCAGCGCGAATCCTGCCTGGCATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCAcccc                                         <  2:3621661/140‑1 (MQ=255)

                                                                                               gggATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAg                                      <  1:4293491/140‑1 (MQ=255)

                                                                                               gggATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAg                                      <  1:90092/140‑1 (MQ=255)

                                                                                               gggATCACCAGCGCGAATCCTGCCTCGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAg                                      <  1:751878/140‑1 (MQ=255)

                                                                                                ggATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATTACATCCTCGCCACCCCAAGc                                     >  2:1662203/1‑140 (MQ=255)

                                                                                                ggATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGc                                     >  1:2201972/1‑140 (MQ=255)

                                                                                                ggATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCATCGCCACCCCAAGc                                     >  2:2265115/1‑140 (MQ=255)

                                                                                                 gATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGATGGCCAGTAACAATAAAAATGACATCCTCGCCACCTCAAGCa                                    >  2:1374814/1‑140 (MQ=255)

                                                                                                  aTCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCAt                                   >  2:2203000/1‑140 (MQ=255)

                                                                                                   tCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCAtg                                  >  2:515813/1‑139 (MQ=255)

                                                                                                    cACCAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCg                                 <  1:2512621/140‑1 (MQ=255)

                                                                                                       cAGCGCGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCAAGGCCGCAGGCTTCTTTAATGGCGACTGGGCAGTACTCCTTACCGCGCTTGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCACCGTaa                              <  2:5327999/140‑1 (MQ=255)

                                                                                                         gcgcGAATCCTGCCTGGGATATGTTTAGCGGCTACGATGGCATGGCCGTAGGCTTCTTCTATGGCGACTGGGGAGTACTGCGTACCGCGCTGGCCAGTAACAATGAAGATGACATCCTCGCCACCCCAAGCATCGTAACg                            <  2:644490/140‑1 (MQ=255)

                                                                                                         gcgcGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACg                            <  1:2682316/140‑1 (MQ=255)

                                                                                                         gcgcGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACg                            <  1:2629416/140‑1 (MQ=255)

                                                                                                         gcgcGAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACg                            <  2:1977307/140‑1 (MQ=255)

                                                                                                          cgcgAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGAGATCCTCGCCACCCCAAGCATCGTAACGc                           >  1:4891293/1‑140 (MQ=255)

                                                                                                          cgcgAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACgg                           >  1:2264642/1‑139 (MQ=255)

                                                                                                          cgcgAATCCTGCCTGGGAGAGGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGc                           >  1:3212912/1‑140 (MQ=255)

                                                                                                            cgAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTg                         >  2:1194311/1‑140 (MQ=255)

                                                                                                            cgAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTg                         >  2:3287548/1‑140 (MQ=255)

                                                                                                            cgAATCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTg                         >  1:3489041/1‑140 (MQ=255)

                                                                                                              aaTCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGCACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGa                       >  2:4858837/1‑140 (MQ=255)

                                                                                                               aTCCTGCCTGGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGat                      >  1:3044957/1‑140 (MQ=255)

                                                                                                                    gcctgGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGAtaataa                 >  1:1399817/1‑140 (MQ=255)

                                                                                                                    gcctgGGATATGTTTAGCGCCTACAATGGCATGGCCGCACGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGAtaagaa                 >  1:4608319/1‑140 (MQ=255)

                                                                                                                      ctgGGATATGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAAc               <  1:4917364/140‑1 (MQ=255)

                                                                                                                           atatGTTTAGCGCCTACGATGGCATGGCCGCCGGCTTCTTCAACGGCGACCGGGGCGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAACCATCGTAACGCTAGATAATAAACTcgcg          <  2:1093619/140‑1 (MQ=255)

                                                                                                                           atatGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTcgcg          <  1:5011572/140‑1 (MQ=255)

                                                                                                                           atatGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTcgcg          <  1:2037922/140‑1 (MQ=255)

                                                                                                                           atatGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTcgcg          <  1:3284559/140‑1 (MQ=255)

                                                                                                                           atatGTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTCACCGCGCTGGCCAGTAACAATAAAAATGACACGCTCGCCACCCCAAGCATCGTAACGCTGGATAATAACCTcgcg          <  2:336660/140‑1 (MQ=255)

                                                                                                                               gTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCTCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAACATCGTAACGCTGGATAATAAACTCGCGTCCt      >  2:2841893/1‑140 (MQ=255)

                                                                                                                               gTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTCGCGTCCt      >  1:2442174/1‑140 (MQ=255)

                                                                                                                               gTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTCGCGTCCt      >  2:5211345/1‑140 (MQ=255)

                                                                                                                               gTTTAGCGCCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTCGCGTCCt      >  1:655164/1‑140 (MQ=255)

GGGCGAATAAAAACGTTGGCGCACAGCAATTTACCAATACCGGATTACCGATTTTTAACGCTGCGCAAGGTGTGGCTGATTATAAAAAGAATGGTGGGATCACCAGCGCGAATCCTGCCTGGGATATGTTTAGCACCTACAATGGCATGGCCGCAGGCTTCTTCAATGGCGACTGGGGAGTACTGCTTACCGCGCTGGCCAGTAACAATAAAAATGACATCCTCGCCACCCCAAGCATCGTAACGCTGGATAATAAACTCGCGTCCTTCAA  >  NZ_CP025268/3458168‑3458438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`