Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,483,653 C→T P560L (CCG→CTG)  yheS → ABC transporter ATP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,483,6530CT99.3% 989.1 / NA 275P560L (CCG→CTG) yheSABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (161/111);  minor base G (1/1);  total (163/112)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 278 total aligned reads displayed.

TATCAACAGTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGCCTGCCTGCAAC  >  NZ_CP025268/3483517‑3483787
                                                                                                                                        |                                                                                                                                      
 aTCAACAGTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGt                                                                                                                                    >  2:2987632/1‑140 (MQ=255)
  tCAACAGTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTa                                                                                                                                   <  2:1405236/140‑1 (MQ=255)
  tCAACAGTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTa                                                                                                                                   >  1:3076416/1‑140 (MQ=255)
  tCAACAGTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTa                                                                                                                                   <  1:2786774/140‑1 (MQ=255)
  tCAACAGTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTa                                                                                                                                   <  2:3348726/140‑1 (MQ=255)
        gTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAgaga                                                                                                                             >  2:4002605/1‑140 (MQ=255)
         tGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGAt                                                                                                                            >  1:5041183/1‑140 (MQ=255)
         tGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGAt                                                                                                                            >  1:4178047/1‑140 (MQ=255)
         tGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGAt                                                                                                                            >  1:840101/1‑140 (MQ=255)
         tGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGAt                                                                                                                            >  1:1854596/1‑140 (MQ=255)
         tGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGAt                                                                                                                            >  2:4024771/1‑140 (MQ=255)
         tGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACAGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGATGCGTGCGCAAACCCAGCCACTGCGTAAAGAGAt                                                                                                                            >  1:3321182/1‑140 (MQ=255)
          ggTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGAtt                                                                                                                           >  2:5274164/1‑140 (MQ=255)
           gTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTg                                                                                                                          >  1:4132140/1‑140 (MQ=255)
           gTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTg                                                                                                                          >  2:4183054/1‑140 (MQ=255)
            ttGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGc                                                                                                                         >  1:2525190/1‑140 (MQ=255)
            ttGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGGAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGGGCTAACCCATGCACTGCGGAAAGAGATTGc                                                                                                                         >  1:4326107/1‑140 (MQ=255)
             tGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTACAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGcc                                                                                                                        <  2:2465969/140‑1 (MQ=255)
                gCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGGCCGt                                                                                                                     >  2:1922981/1‑140 (MQ=255)
                 cGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTc                                                                                                                    >  1:2567316/1‑140 (MQ=255)
                 cGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTc                                                                                                                    <  1:2148529/140‑1 (MQ=255)
                   aCGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTg                                                                                                                  >  1:4111848/1‑140 (MQ=255)
                   aCGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCGGAGCTGCGTGCCCACACCCAGCCACTGCGAAAAGAGGTCGCCCgacgg                                                                                                                  >  2:3971250/1‑136 (MQ=255)
                    cGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTgg                                                                                                                 >  2:4028134/1‑140 (MQ=255)
                     gTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGa                                                                                                                >  2:3298453/1‑140 (MQ=255)
                       aCAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGaaa                                                                                                              <  2:3615170/140‑1 (MQ=255)
                          aaaGCAGGAAAACCAGCCCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAag                                                                                                           <  2:3847691/140‑1 (MQ=255)
                             gCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAgaga                                                                                                        <  1:1577513/140‑1 (MQ=255)
                              cAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAGTGCGAACAGCGCCCAGGAAGGTAAAGATCAGAGGCGGCGGGAAGCTGAGCGGCGGGGGCAAAGCCAGCCACTGCGTAAGGAGATGGCGCGGCGGGAAAAAgagag                                                                                                       >  2:5133598/1‑139 (MQ=255)
                              cAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGAt                                                                                                       >  2:2029611/1‑140 (MQ=255)
                              cAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGAt                                                                                                       >  2:1363988/1‑140 (MQ=255)
                              cAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGAt                                                                                                       >  1:3954468/1‑140 (MQ=255)
                              cAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGCAAAAAGAGAt                                                                                                       >  2:3455802/1‑140 (MQ=255)
                                ggAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATgg                                                                                                     >  2:450136/1‑140 (MQ=255)
                                ggAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATgg                                                                                                     >  1:614256/1‑140 (MQ=255)
                                ggAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATgg                                                                                                     >  2:3212806/1‑140 (MQ=255)
                                 gAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGga                                                                                                    >  1:1982094/1‑140 (MQ=255)
                                  aaaaCCAGACCGACGAAGCGACACAAGGGCATGCGAAAAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGGTGAGCGGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATggtg                                                                                                   <  2:695037/140‑3 (MQ=255)
                                      ccAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGc                                                                                               >  1:5293967/1‑140 (MQ=255)
                                       ccgACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCt                                                                                              <  2:1520874/138‑1 (MQ=255)
                                        aGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACACAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTg                                                                                             >  2:87016/1‑140 (MQ=255)
                                          aCCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGaa                                                                                           >  1:2267478/1‑140 (MQ=255)
                                            cgacgaAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAAcg                                                                                         <  1:4964377/140‑1 (MQ=255)
                                               cgaAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCAAGCCACTGCGTAAAGAGATTGCGCGTCTGGAAAATGAGATGGAGAAGCTGAACgcgc                                                                                      >  2:2952515/1‑140 (MQ=255)
                                                    cgcCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTg                                                                                 >  1:1126767/1‑140 (MQ=255)
                                                      ccAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGgc                                                                               >  2:185041/1‑140 (MQ=255)
                                                        aaaaGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAACGAGATGGAGAAGCTGAACGCGCAACTGgcgc                                                                             <  2:4155005/140‑1 (MQ=255)
                                                            gagaATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGc                                                                         <  1:4919872/140‑1 (MQ=255)
                                                            gagaATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGc                                                                         <  2:2525190/140‑1 (MQ=255)
                                                            gagaATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGc                                                                         <  1:3259178/140‑1 (MQ=255)
                                                                    gAACCGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAGCCCAGCCACTGCGTAAAGAGATTGCCCGGCTGGAAAAAGAGATGGACAAGCTGAACGCGCAACTGGCGCAGGCGGAAgaga                                                                 <  2:2674811/140‑1 (MQ=255)
                                                                    gAACAGCGCCCAGGCAAGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACGGGCGCAGGCGGAAgaga                                                                 <  1:3979509/140‑1 (MQ=255)
                                                                     aaCAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTTCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGaa                                                                >  1:242094/1‑140 (MQ=255)
                                                                      aCAGCGCCCAGGCACGTAAAGATAAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGACGCTGAACGCGCAACTGGCGCAGGCGGAAGAGaaa                                                               <  1:2852210/140‑1 (MQ=255)
                                                                         gcgcCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTc                                                            >  1:1316474/1‑140 (MQ=255)
                                                                         gcgcCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTc                                                            >  2:1010597/1‑140 (MQ=255)
                                                                            cccAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGc                                                         >  1:1083212/1‑140 (MQ=255)
                                                                               aGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGAc                                                      >  1:5185215/1‑140 (MQ=255)
                                                                                   aCGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCg                                                  <  1:2928166/140‑1 (MQ=255)
                                                                                    cGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGTAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                 <  2:159576/140‑1 (MQ=255)
                                                                                    cGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                 <  1:1336309/140‑1 (MQ=255)
                                                                                    cGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                 <  2:2764196/140‑1 (MQ=255)
                                                                                    cGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                 <  1:450136/140‑1 (MQ=255)
                                                                                    cGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                 <  1:572631/140‑1 (MQ=255)
                                                                                    cGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGa                                                 <  1:898909/140‑1 (MQ=255)
                                                                                    cGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGACTGCCGGTCCGGAAAAAGAGATGGAGAAACAGAACGCGCAATTGGCGCAGGCGCAAGAGAACCTCGGCGACAGcta                                                 <  2:1161272/140‑3 (MQ=255)
                                                                                     gTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGaa                                                <  1:3144497/140‑1 (MQ=255)
                                                                                      tAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCg                                                  >  2:2878921/1‑137 (MQ=255)
                                                                                           aTCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTAt                                          >  1:4731015/1‑140 (MQ=255)
                                                                                           aTCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTAt                                          >  1:3829599/1‑140 (MQ=255)
                                                                                           aTCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTAt                                          >  1:3439997/1‑140 (MQ=255)
                                                                                            tCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATg                                         >  1:4561726/1‑140 (MQ=255)
                                                                                              aGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGAc                                       <  1:4115711/140‑1 (MQ=255)
                                                                                              aGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGAc                                       <  1:3053672/140‑1 (MQ=255)
                                                                                               gAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGAcc                                      >  1:2405149/1‑140 (MQ=255)
                                                                                               gAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGAcc                                      >  2:599666/1‑140 (MQ=255)
                                                                                                aaGCGTCGGGAAGCTGCGCAGCGTGCGCAAACCCAGCCACTGCGTAAAGAGACTGCCCGTCTGGAAAAAGAGGTGGAGAAGCTGAACGCGCAACTGCCGCAGGCGGAAGAGATACTCGGCGACAGCGAACTGTATGACCa                                     <  2:5060424/140‑1 (MQ=255)
                                                                                                aaGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCa                                     <  2:1105989/140‑1 (MQ=255)
                                                                                                aaGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCa                                     <  1:2883964/140‑1 (MQ=255)
                                                                                                aaGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCa                                     >  2:3086509/1‑140 (MQ=255)
                                                                                                   cGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACATCGAACTGTATGACCagag                                  <  2:4670418/140‑1 (MQ=255)
                                                                                                   cGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCagag                                  >  1:2073029/1‑140 (MQ=255)
                                                                                                     tCGGGAAGCTGAGCTGCGTGCGTAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAACGATATGGAGAAGCTGAACGCGAAACTGGCGCAGGCGGAAGAGAAGCTCGTGGACAGCGGTCGGTATGACCAGAGcc                                >  2:1678173/1‑140 (MQ=255)
                                                                                                     tCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGcc                                >  1:341863/1‑140 (MQ=255)
                                                                                                             ctgagctgCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCg                        >  2:2205028/1‑140 (MQ=255)
                                                                                                                agctgCGTGCGCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGACCGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAg                     >  2:224194/1‑140 (MQ=255)
                                                                                                                  cggtgtgcgCAAACACATCCACTGCACAAAGAGATTCCCCGTCTCGAAAAAGAGATGGAGAAGCGGAACGCGGTACAGGCGCAGGCGGACGAAACACTATGCGCCAGCGAACTGTATGACCAGCGACGTAAAGCGGAGtt                   <  2:1190135/136‑1 (MQ=255)
                                                                                                                     cgtgcgCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCg                                                  <  1:1675/106‑1 (MQ=255)
                                                                                                                     cgtgcgCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGAc                >  1:2253201/1‑140 (MQ=255)
                                                                                                                     cgtgcgCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGCAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACACGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGGTGAc                >  2:4632988/1‑140 (MQ=255)
                                                                                                                       tgcgCAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGGAAAGCGGAGTTGAccc              >  2:4414718/1‑139 (MQ=255)
                                                                                                                        gcgcAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCgc             <  2:476163/140‑1 (MQ=255)
                                                                                                                          gcACACCCAGCCAGTGCGTAAAGAGCTTGCCCGTCTGGAACAAGAGGTGGGGAAGCTGAGCGCGCAACTGGCGCGGGCGGAAGAGGAACTCGGCGTCAGCGAACTGTATGACCAGAGCCGTAAGGCGGAGTTGACCgcct           <  2:2726545/140‑1 (MQ=255)
                                                                                                                          gcAAACCCAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCgcct           <  1:177622/140‑1 (MQ=255)
                                                                                                                               cccAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTCTGACCAGAGCCGTAAAGCGGAGTTGACCGcctgcctg      >  2:4975533/1‑140 (MQ=255)
                                                                                                                               cccAGCCACTGCGTAAAGAGATGGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGcctgcctg      >  1:4067842/1‑140 (MQ=255)
                                                                                                                                ccAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGCctgcctgc     <  1:1440901/140‑1 (MQ=255)
                                                                                                                                ccAGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGCctgcctgc     <  1:1655407/140‑1 (MQ=255)
                                                                                                                                  aGCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGCCTGCCTGCaa   >  1:2669572/1‑140 (MQ=255)
                                                                                                                                   gCCACTGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGAGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGCGCCGAAAAGAGGCGTTGACCGCCTGCCTGCAAc  >  2:4210/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                      
TATCAACAGTGGTTGAGCGACGTACAAAAGCAGGAAAACCAGACCGACGAAGCGCCAAAAGAGAATGCGAACAGCGCCCAGGCACGTAAAGATCAGAAGCGTCGGGAAGCTGAGCTGCGTGCGCAAACCCAGCCACCGCGTAAAGAGATTGCCCGTCTGGAAAAAGAGATGGAGAAGCTGAACGCGCAACTGGCGCAGGCGGAAGAGAAACTCGGCGACAGCGAACTGTATGACCAGAGCCGTAAAGCGGAGTTGACCGCCTGCCTGCAAC  >  NZ_CP025268/3483517‑3483787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑