Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,508,628 G→A pseudogene (648/878 nt) php ← phosphotriesterase‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,508,6280GA99.4% 1276.3 / NA 348pseudogene (648/878 nt)phpphosphotriesterase‑related protein
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (238/106);  total (242/106)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 353 total aligned reads displayed.

TAGTAAATAGTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCGTGGGCTTGTA  >  NZ_CP025268/3508491‑3508762
                                                                                                                                         |                                                                                                                                      
tAGTAAATAGTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTGAGCAACCCACGGTCGCGTAGCGCATGAAGCAGCGCAATAAGCTTTCCGTCCGggtagt                                                                                                                                      >  2:3586463/1‑140 (MQ=255)
 aGTAAATAGTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGgtagta                                                                                                                                     >  2:3675640/1‑140 (MQ=255)
   tAAATAGTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAAc                                                                                                                                   >  1:2753496/1‑140 (MQ=255)
     aaTAGTCATAGCCATAACCACCGTTGGCTTTTAGATGGGAGCGGCGCGTAATATCCATCGACAGCACGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTCCGTCCGGGTAGTAACTg                                                                                                                                 >  2:1104525/1‑140 (MQ=255)
     aaTAGTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATAAGCTTTTCGTCCGGGTAGTAACTg                                                                                                                                 <  1:2239071/140‑1 (MQ=255)
      aTAGTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATAACCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACttt                                                                                                                                >  2:5351553/1‑138 (MQ=255)
       tAGTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCGATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGtt                                                                                                                               <  2:5183267/140‑1 (MQ=255)
       tAGTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGtt                                                                                                                               <  1:498133/140‑1 (MQ=255)
       tAGTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCAACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGtt                                                                                                                               <  2:238476/140‑1 (MQ=255)
        aGTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTc                                                                                                                              >  1:1566892/1‑140 (MQ=255)
        aGTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTc                                                                                                                              >  1:906559/1‑140 (MQ=255)
          tCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCCTGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCtt                                                                                                                            >  1:1008113/1‑140 (MQ=255)
            aTAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGc                                                                                                                          >  1:718010/1‑140 (MQ=255)
            aTAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAACACGCTTTTCGTCCGGGTAGTAACTGTTCTTGc                                                                                                                          >  1:1245220/1‑140 (MQ=255)
             tAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGcc                                                                                                                         >  1:3457207/1‑140 (MQ=255)
             tAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGcc                                                                                                                         >  2:916339/1‑140 (MQ=255)
             tAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGcc                                                                                                                         >  1:4131556/1‑140 (MQ=255)
             tAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGcc                                                                                                                         >  2:1739409/1‑140 (MQ=255)
             tAGCCATAACCACCGGTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGcc                                                                                                                         >  2:1500278/1‑140 (MQ=255)
               gCCATAACCACCGTTGGCTTTTAAATGGGGCCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGa                                                                                                                       <  2:1916040/140‑1 (MQ=255)
               gCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTCATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGa                                                                                                                       <  1:94422/140‑1 (MQ=255)
               gCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCCTCGCAATACGCTTTTCGTCCGGGCAGTAACTGTTCTTGCCGa                                                                                                                       <  1:3749425/140‑1 (MQ=255)
               gCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGa                                                                                                                       <  2:5070338/140‑1 (MQ=255)
                 cacaaCCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATg                                                                                                                     <  2:4511811/137‑1 (MQ=255)
                    aaCCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGGATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGgtg                                                                                                                  >  2:5035882/1‑140 (MQ=255)
                    aaCCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGgtg                                                                                                                  >  1:5129783/1‑140 (MQ=255)
                    aaCCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGgtg                                                                                                                  >  1:2498727/1‑140 (MQ=255)
                    aaCCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGgtg                                                                                                                  >  1:586200/1‑140 (MQ=255)
                    aaCCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGgtg                                                                                                                  >  1:5312019/1‑140 (MQ=255)
                    aaCCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGgtg                                                                                                                  >  1:368214/1‑140 (MQ=255)
                    aaCCACCGTTGGCTTTTAAAGGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCAGCGCAATACGCGGCTCGTCCGGGTAGTAACTGTTCTTGCGGATGgtg                                                                                                                  >  2:1480088/1‑140 (MQ=255)
                       caccGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCg                                                                                                               <  1:3527168/140‑1 (MQ=255)
                          cGTTGGCTTTTGAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTATTGCCGATGGTGTCGAAc                                                                                                            >  2:3798349/1‑140 (MQ=255)
                          cGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGTTGTCGAAc                                                                                                            >  1:1930914/1‑140 (MQ=255)
                          cGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAAc                                                                                                            >  2:4052976/1‑140 (MQ=255)
                          cGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAAc                                                                                                            >  1:1156998/1‑140 (MQ=255)
                          cGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAAc                                                                                                            >  2:189257/1‑140 (MQ=255)
                          cGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAAc                                                                                                            >  1:2634714/1‑140 (MQ=255)
                          cGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAAc                                                                                                            >  2:3487983/1‑140 (MQ=255)
                           gTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCCCGCTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACt                                                                                                           >  1:5066888/1‑140 (MQ=255)
                                  tttCAATGGCAGCGGCGCGTAATATACATCGCCAGCATGACGCGGTTCAGCAACACACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTa                                                                                                    <  2:158778/140‑1 (MQ=255)
                                  tttAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTa                                                                                                    <  1:4751037/140‑1 (MQ=255)
                                  tttAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTa                                                                                                    <  2:4660029/140‑1 (MQ=255)
                                    tAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTAcg                                                                                                  >  2:5130395/1‑140 (MQ=255)
                                           gAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCgag                                                                                           >  1:1550578/1‑140 (MQ=255)
                                           gAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCgag                                                                                           >  1:3283191/1‑140 (MQ=255)
                                           gAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCgag                                                                                           >  1:3151141/1‑140 (MQ=255)
                                              cggcgCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAgatc                                                                                        >  1:2525111/1‑140 (MQ=255)
                                                 cgcgTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAgatcgat                                                                                     >  2:688933/1‑140 (MQ=255)
                                                  gcgTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAgatcgatc                                                                                    <  1:3883629/140‑1 (MQ=255)
                                                   cgTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGatca                                                                                   >  2:3456985/1‑140 (MQ=255)
                                                         atCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCa                                                                             <  1:2680661/140‑1 (MQ=255)
                                                           ccATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCaaa                                                                           >  1:2990342/1‑140 (MQ=255)
                                                           ccATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCaaa                                                                           >  1:3591408/1‑140 (MQ=255)
                                                            cATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCaaaa                                                                          <  1:2080218/140‑1 (MQ=255)
                                                             aTCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAAt                                                                         >  1:3342289/1‑140 (MQ=255)
                                                             aTCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAAt                                                                         >  1:4205092/1‑140 (MQ=255)
                                                              tCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGAGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAAtg                                                                        >  2:2526069/1‑140 (MQ=255)
                                                                  cAGCATGCCGCGGTTCAGAAACCAACGTGCGCGTAGAGCATGAGGGGTCGCAATACGCTTTTCGTCCGTGTAGTAACTGTTCTTGCCGATGGTGTCGAACTTCATGTACGCGCCGAGATGGATCAGTTTCGAAAggttgt                                                                    >  2:4334097/1‑140 (MQ=255)
                                                                    gCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCg                                                                  >  1:2918820/1‑140 (MQ=255)
                                                                      aTGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAACGTTGTCGAg                                                                >  1:818800/1‑140 (MQ=255)
                                                                               ttCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCttt                                                       >  2:1363345/1‑140 (MQ=255)
                                                                               ttCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCttt                                                       >  1:3603479/1‑140 (MQ=255)
                                                                               ttCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCttt                                                       >  2:4408044/1‑140 (MQ=255)
                                                                               ttCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCGTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAGCTGCACGTACGCGCCGCGATCGATCACCTTCAAAACGTTGTCGAGGTTGTCttt                                                       >  2:4254895/1‑140 (MQ=255)
                                                                               ttCAGCAACCCACAGTCGCGTAGCGCATGAAGCATCGCAATACGGTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATGATCTTCAAAAGGTTGTCGAGGTGGTCtgt                                                       >  2:3775254/1‑138 (MQ=255)
                                                                                   gCAACCCACGGTCGCCTAGCGCATGAATAATCGCACTACGCTTTTCCTCCGGGTAGTAACTGTTCTTGCCGATGGTGCCGAACTGCAAGTACGCGACGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTATTTCAGa                                                   <  2:2853041/140‑1 (MQ=255)
                                                                                      aCCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCg                                                <  1:399379/140‑1 (MQ=255)
                                                                                        ccACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCa                                              <  1:4543433/140‑1 (MQ=255)
                                                                                        ccACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATCGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCa                                              <  2:4930644/140‑1 (MQ=255)
                                                                                         cACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAg                                             >  1:2873673/1‑140 (MQ=255)
                                                                                         cACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAg                                             >  1:4643754/1‑140 (MQ=255)
                                                                                            ggTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGa                                          >  2:2199706/1‑140 (MQ=255)
                                                                                             gTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGAc                                         <  1:1758422/140‑1 (MQ=255)
                                                                                             gTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGAc                                         <  1:4339693/140‑1 (MQ=255)
                                                                                                 cgTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAAc                                     >  2:3223104/1‑140 (MQ=255)
                                                                                                  gTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACg                                    >  2:1259774/1‑140 (MQ=255)
                                                                                                      cgcATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGa                                >  2:3424256/1‑140 (MQ=255)
                                                                                                        cATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGAc                                         >  2:3916927/1‑129 (MQ=255)
                                                                                                        cATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGAcg                              >  2:1233385/1‑140 (MQ=255)
                                                                                                        cATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGAcg                              >  2:3114353/1‑140 (MQ=255)
                                                                                                        cATGAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGGTGGCTTTCAGATCGCAGGGACCAACGGTTAcg                              >  2:4807718/1‑140 (MQ=255)
                                                                                                           gAAGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGAcgcgc                           >  2:3956732/1‑140 (MQ=255)
                                                                                                             aGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGa                         <  2:5255627/140‑1 (MQ=255)
                                                                                                             aGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGa                         <  1:1445804/140‑1 (MQ=255)
                                                                                                             aGCATCGCAATACGCTTTTCGTCCGGGTAGTAACTGATCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGa                         <  1:5278065/140‑1 (MQ=255)
                                                                                                                aTCGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCCGTGACCAACGGCGACGCGCGAAAg                      >  2:170445/1‑140 (MQ=255)
                                                                                                                aTCGCAATACGCTTTTCGCCCGGGTAGTCACTGTTCTTGCCGACGGTGTCGAACTCCACGTACGCGCCGCGATCGATCAGCTTCAAAATGTTGCCTAGGTTGCCCTGCACATCGCACCGACCAACAGTGACGCGCGAAAg                      >  2:3331731/1‑140 (MQ=255)
                                                                                                                 tCGCAATACGCTGTTCGTCCGGGTAGTAACTGTTCTTGCCGGTGCTGTCGAACTGCACGTACGCGCCGAGATCGGTCATCTTCAATATGCTGTCGTGGTTGTCTTGCAGATCGCCGTGACCAGCGGTGACGCGCGAAAGa                     >  2:329059/1‑140 (MQ=255)
                                                                                                                  cGCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGAt                    >  1:4842232/1‑140 (MQ=255)
                                                                                                                   gCAATACGCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATc                   <  1:520698/140‑1 (MQ=255)
                                                                                                                          gCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCg            <  1:4947939/140‑1 (MQ=255)
                                                                                                                          gCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCg            <  2:2418309/140‑1 (MQ=255)
                                                                                                                          gCTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACACGCGAAAGATCAACCCCg            <  2:813915/140‑1 (MQ=255)
                                                                                                                           cTTTTCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCGt           <  1:2184832/140‑1 (MQ=255)
                                                                                                                            ttttCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCGAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCGTg          <  2:2945398/140‑1 (MQ=255)
                                                                                                                             tttCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCGTgg         <  2:1404492/140‑1 (MQ=255)
                                                                                                                              ttCGTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCGTggg        >  2:2108692/1‑140 (MQ=255)
                                                                                                                                 gTCCGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCGTGGGCtt     <  2:1295782/140‑1 (MQ=255)
                                                                                                                                   ccGGGTAGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCGTGGGCTTGt   >  1:1610738/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                      
TAGTAAATAGTCATAGCCATAACCACCGTTGGCTTTTAAATGGGAGCGGCGCGTAATATCCATCGACAGCATGACGCGGTTCAGCAACCCACGGTCGCGTAGCGCATGAAGCATCGCAATACGCTTTTCGTCCGGGTGGTAACTGTTCTTGCCGATGGTGTCGAACTGCACGTACGCGCCGAGATCGATCATCTTCAAAATGTTGTCGAGGTTGTCTTTCAGATCGCAGTGACCAACGGTGACGCGCGAAAGATCAACCCCGTGGGCTTGTA  >  NZ_CP025268/3508491‑3508762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑