Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,532,291 C→T A279T (GCA→ACA)  yhgE ← DUF4153 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,532,2910CT99.2% 940.5 / NA 263A279T (GCA→ACA) yhgEDUF4153 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (137/122);  total (140/123)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 265 total aligned reads displayed.

CACCGCCAGCACGCCCTGCAAGCGGTCGACAGTCCAGCCGTACTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGCCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGAT  >  NZ_CP025268/3532154‑3532429
                                                                                                                                         |                                                                                                                                          
cACCGCCAGCACGCCCTGCAAGCGGTCGACAGTCCAGCCGTACTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGtcc                                                                                                                                          >  2:3423970/1‑140 (MQ=255)
  ccGCCAGCACGCCCTGCAAGCGGTCGACAGTCCAGCCGTACTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAg                                                                                                                                        <  2:540087/140‑1 (MQ=255)
     cccgCACGCGCTGCAGGCGGTCGACAGTCCAGCCGTACGGAGCGCCCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTTATCAGCCAACGCAACGGCCCTGTCCAGGGa                                                                                                                                     >  2:272115/4‑140 (MQ=255)
     ccAGCACGCCCTGCAAGCGGTCGACAGTCCAGCCGTACTGAGCGACCCGCAGCCATAACGCCCAGGCGTCGACGAACACATACAGCGGAGCAACCAACAAAGCGGTTTTAATCAGGCAACGCCACGGCCCTGTCCAGGGa                                                                                                                                     >  2:4872507/1‑140 (MQ=255)
     ccAGCACGCCCTGCAAGCGGTCGACAGTCCAGCCGTACTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGa                                                                                                                                     >  2:1718282/1‑140 (MQ=255)
         cACGCCCTGCAAGCGGTCGACAGTCCAGCCGTACTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTCTCCAGGGAAGTg                                                                                                                                 >  2:3770418/1‑140 (MQ=255)
         cACGCCCTGCAAACGTACGACAGTCCAGCCGTACCGAGCGACCCGAAGCCATAACGCCCAGACAGCCACGAACACAAACAGCGGAGCAACCAGCAAAGCGTTATTAATCAGGCAACGCAACGACCCTGCCCAGGGAAGTg                                                                                                                                 >  2:5028201/1‑140 (MQ=255)
           cGCCCTGCAAGCGGTCGACAGTCCAGCCGTACTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAGGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGAc                                                                                                                               <  2:1112439/140‑1 (MQ=255)
             cccTGCAAGCGGTCGACAGTCCAGCCGTACTTAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGc                                                                                                                             >  2:2843350/1‑140 (MQ=255)
             cccTGCAAGCGGTCGACAGTCCAGCCGTACTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGc                                                                                                                             >  2:3380041/1‑140 (MQ=255)
                   aaGCGGTCGACAGTCCAGCCGTACTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTg                                                                                                                       <  1:5121950/140‑1 (MQ=255)
                    aGCGGTCGACAGTCCAGCCGTACTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGc                                                                                                                      <  1:3098613/140‑1 (MQ=255)
                        gTCGACAGTCCAGCCGTACTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCCAAGCGGTTTTAATCAGGCACCGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGAt                                                                                                                  >  1:1416289/1‑140 (MQ=255)
                        gTCGACAGTCCAGCCGTACTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGAt                                                                                                                  >  1:1288650/1‑140 (MQ=255)
                        gTCGACAGTCCAGCCGTACTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGAt                                                                                                                  >  2:2049967/1‑140 (MQ=255)
                        gTCGACAGTCCAGCCGTACTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGAt                                                                                                                  >  1:4697201/1‑140 (MQ=255)
                        gTCGACAGTCCAGCCGTACTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGAt                                                                                                                  >  1:3223282/1‑140 (MQ=255)
                          cGACAGTCCAGCCGTACTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATcg                                                                                                                <  1:5187524/140‑1 (MQ=255)
                          cGACAGTCCAGCCGTACTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATcg                                                                                                                <  1:3605493/140‑1 (MQ=255)
                            aCAGTCCAGCCGTACTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATcgcg                                                                                                              <  1:5059273/140‑1 (MQ=255)
                              aGTCCAGCCGTACTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGa                                                                                                            <  1:372682/140‑1 (MQ=255)
                                 ccAGCCGTACTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACaa                                                                                                         <  1:215298/140‑1 (MQ=255)
                                 ccAGCCGTACTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACaa                                                                                                         <  1:2063463/140‑1 (MQ=255)
                                  cAGCCGTACTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAAt                                                                                                        <  1:3287492/140‑1 (MQ=255)
                                   aGCCGTACTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATa                                                                                                       <  2:4816803/140‑1 (MQ=255)
                                   aGCCGTACTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATa                                                                                                       >  1:822405/1‑140 (MQ=255)
                                   aGCCGTACTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATa                                                                                                       >  1:2374250/1‑140 (MQ=255)
                                    gCCGTACTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAg                                                                                                      <  1:1448272/140‑1 (MQ=255)
                                       gTACTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCa                                                                                                   <  1:2558136/140‑1 (MQ=255)
                                         aCTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCAtt                                                                                                 <  2:1339331/140‑1 (MQ=255)
                                          cTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACGTACGGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTa                                                                                                >  1:3745080/1‑140 (MQ=255)
                                          cTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACTGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTa                                                                                                >  2:4641544/1‑140 (MQ=255)
                                          cTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTa                                                                                                >  1:4140972/1‑140 (MQ=255)
                                          cTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTa                                                                                                >  2:25644/1‑140 (MQ=255)
                                          cTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTa                                                                                                >  1:2119917/1‑140 (MQ=255)
                                          cTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTa                                                                                                >  1:19702/1‑140 (MQ=255)
                                          cTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGATTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTa                                                                                                >  2:4765097/1‑140 (MQ=255)
                                            gAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAg                                                                                              <  1:4902092/140‑1 (MQ=255)
                                             aGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGa                                                                                             >  2:2482985/1‑140 (MQ=255)
                                               cGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACTGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCGACGGCCCTGTGCAGGTAAGTGACGCTTTTTGCGGATCGCGGACAATAGGCATTAAGATc                                                                                           >  1:2920605/1‑140 (MQ=255)
                                               cGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATc                                                                                           >  2:1027296/1‑140 (MQ=255)
                                                 aCCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCaa                                                                                         <  2:4271028/140‑1 (MQ=255)
                                                  cccGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAAt                                                                                        >  1:983626/1‑140 (MQ=255)
                                                  cccGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAAt                                                                                        >  2:2976546/1‑140 (MQ=255)
                                                  cccGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAAt                                                                                        >  1:1597900/1‑140 (MQ=255)
                                                  cccGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAACAGCCATTAAGATCAAt                                                                                        >  1:2970211/1‑140 (MQ=255)
                                                    cGCAGCCCTAACGACCAGGCGGCGACGAACACATACAGCGGCGCAACCCGCAAAGCGGTATTAATAAGGCAACGCAACGGCCCTGGCCAGGGAAGGGCCGCTTGTTGCGGATCGGGGACAAGAGCCAGTAAGATCAACTg                                                                                      >  2:2632304/1‑140 (MQ=255)
                                                            tAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAgg                                                                              >  1:537331/1‑140 (MQ=255)
                                                              aCGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGcc                                                                            <  2:2182160/140‑1 (MQ=255)
                                                               cGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCTGCAAAGCGGTTTTAATCAAGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCa                                                                           <  2:492911/140‑1 (MQ=255)
                                                                 ctcAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGGGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGc                                                                         >  1:4283882/3‑140 (MQ=255)
                                                                 cccAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGc                                                                         >  1:3518242/1‑140 (MQ=255)
                                                                   cAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGt                                                                                                                                  >  2:523267/1‑81 (MQ=255)
                                                                    aGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTc                                                                      >  1:3337429/1‑140 (MQ=255)
                                                                     ggcggcGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCa                                                                     >  2:1845308/1‑140 (MQ=255)
                                                                      gcggcgACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCaa                                                                    <  1:2798440/140‑1 (MQ=255)
                                                                                 cacaTACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGTCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCcgg                                                         <  2:1264067/140‑1 (MQ=255)
                                                                                 cacaTACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATAGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCcgg                                                         <  1:405569/140‑1 (MQ=255)
                                                                                  acaTACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCcggc                                                        <  2:5043050/140‑1 (MQ=255)
                                                                                    aTACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCcggcgg                                                      >  2:1214098/1‑140 (MQ=255)
                                                                                         gCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGgag                                                 >  2:288318/1‑140 (MQ=255)
                                                                                          cGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGGCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGgaga                                                >  1:2695581/1‑140 (MQ=255)
                                                                                          cGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGTCAATAGCCATTAAGAGCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGgcga                                                >  1:4441611/1‑140 (MQ=255)
                                                                                          cGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGgaga                                                >  1:1019073/1‑140 (MQ=255)
                                                                                            taGCAACCAGCAAAGCGGGTTTAATCAGTCAACGCAGCGGCCCTGTCCAGGGAAGTGACGCGTTTTGCGGATCGCGGACAATAGCCATTAAGAGCGAGTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGAtg                                              <  2:4019610/139‑1 (MQ=255)
                                                                                            gAGCAACCAGCAAAGCGGTTTTAATTAGGCAACGCAACGGCCCTCTCCAGGGAAGTGATGCTTTTTTCTGTTCGCGGATTTTGGCCATTAATATCGTTTGTAATAAGGCCAGCGTCATTAGCCAGCcggccgctctgatg                                              >  2:2146426/1‑133 (MQ=255)
                                                                                            gAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGAtg                                              >  2:233262/1‑140 (MQ=255)
                                                                                            gAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGAtg                                              >  1:3529139/1‑140 (MQ=255)
                                                                                            gAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGAtg                                              >  1:3501845/1‑140 (MQ=255)
                                                                                            gAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGAtg                                              >  2:5250379/1‑140 (MQ=255)
                                                                                             aGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGAtgt                                             >  1:3960020/1‑140 (MQ=255)
                                                                                               cAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGATGTgg                                           >  1:1070422/1‑140 (MQ=255)
                                                                                               cAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGACATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGAtgtag                                           >  2:2691448/1‑138 (MQ=255)
                                                                                                       aaaGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCATCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAAt                                   >  1:3438868/1‑140 (MQ=255)
                                                                                                        aaGCGGTTGTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTGTGTGCGGATCGCGGACAATAGCCATTAAGAGCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATc                                  <  2:620271/140‑1 (MQ=255)
                                                                                                         aGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATcg                                 >  2:5083173/1‑140 (MQ=255)
                                                                                                         aGCGGTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATcg                                 >  1:4626364/1‑140 (MQ=255)
                                                                                                             gTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCt                             <  2:5198006/140‑1 (MQ=255)
                                                                                                             gTTTTAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCt                             <  2:3558883/140‑1 (MQ=255)
                                                                                                               tttAATCAGGCACCGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCa                           <  2:571419/140‑1 (MQ=255)
                                                                                                                ttAATCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAg                          >  1:3893226/1‑140 (MQ=255)
                                                                                                                  aaTCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGc                        <  2:1446716/140‑1 (MQ=255)
                                                                                                                  aaTCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGc                        <  2:2005807/140‑1 (MQ=255)
                                                                                                                  aaTCAGGCAACGCAACGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGc                        <  2:3185584/140‑1 (MQ=255)
                                                                                                                  aaTCAGGCAACGCAACGGCCCTGTACAGGGAAGTGACGCTTTTTGCGGATCGCGGCCAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGc                        <  2:1288363/140‑1 (MQ=255)
                                                                                                                     cAGGCAACGCAACGGCCCTGTCCAGGGAAGTCACGCTTGTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCg                     >  1:4773923/1‑140 (MQ=255)
                                                                                                                        gcaacgcaacGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTaa                  >  1:1781626/1‑140 (MQ=255)
                                                                                                                        gcaacgcaacGGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAACCGCGCTCAGGCCCGTaa                  >  2:3252408/1‑140 (MQ=255)
                                                                                                                         caacgcaacgGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTaaa                 <  2:3699972/140‑1 (MQ=255)
                                                                                                                            cgcaacgGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACgg              <  1:1335060/140‑1 (MQ=255)
                                                                                                                            cgcaacgGCCCTGTCCAGGGAAGTGACGCTTTTCGCGGATCTCGGACAATAGCCATTAAGAACACTTGAAAAAAGGCCAGCGGCAACAGCAACCCGGCGGCGGCGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACgg              <  2:1651096/140‑1 (MQ=255)
                                                                                                                             gcaacgGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGc             <  2:3324016/140‑1 (MQ=255)
                                                                                                                              caacgGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCa            <  1:732556/140‑1 (MQ=255)
                                                                                                                                 cgGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGGTCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAggg         <  1:1677123/140‑1 (MQ=255)
                                                                                                                                 cgGCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAggg         <  2:2765858/140‑1 (MQ=255)
                                                                                                                                   gCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGtg       <  2:1638994/140‑1 (MQ=255)
                                                                                                                                   gCCCTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGtg       <  1:4320256/140‑1 (MQ=255)
                                                                                                                                    cccTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGtga      <  1:5091138/140‑1 (MQ=255)
                                                                                                                                    cccTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGtga      <  2:3971461/140‑1 (MQ=255)
                                                                                                                                      cTGTCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGtgatg    >  2:738760/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                          
CACCGCCAGCACGCCCTGCAAGCGGTCGACAGTCCAGCCGTACTGAGCGACCCGCAGCCATAACGCCCAGGCGGCGACGAACACATACAGCGGAGCAACCAGCAAAGCGGTTTTAATCAGGCAACGCAACGGCCCTGCCCAGGGAAGTGACGCTTTTTGCGGATCGCGGACAATAGCCATTAAGATCAATTGCAAAAAGGCCAGCGTCAACAGCAACCCGGCGGCGGAGATGTGGCGAGAAATCGCGCTCAGGCCCGTAAACGGCAGGGTGATGAT  >  NZ_CP025268/3532154‑3532429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑