BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	345,338	C→T	L17L (TTG→TTA)	yahN ←	LysE family transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	345,338	0	C	T	99.4%	591.2 / NA	161	L17L (TTG→TTA)	yahN	LysE family transporter
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (98/62); total (98/63)
Fisher's exact test for biased strand distribution p-value = 3.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 161 total aligned reads displayed.

CCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGAACTAA  >  NZ_CP025268/345201‑345473

cccAGCGCCACTCCCAGCCCGGTCCGCACCCCTGCGCCGCGACCGGAAGCCAGGCTGGTTTGTACTACCACCAAGAGCTTCGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTaa                                                                                                                                       <  2:3972867/140‑1 (MQ=255)

cccAGAGCCACGACCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTaa                                                                                                                                       <  1:156135/140‑1 (MQ=255)

  cAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAg                                                                                                                                     <  1:4850583/140‑1 (MQ=255)

  cAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAg                                                                                                                                     <  1:1039692/140‑1 (MQ=255)

  cAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGGCCTACGGTCAGGTAAACGGCATGTAAAg                                                                                                                                     <  1:1130701/140‑1 (MQ=255)

     cgcCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGAt                                                                                                                                  >  1:3535232/1‑140 (MQ=255)

      gcCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATc                                                                                                                                 <  1:3883068/140‑1 (MQ=255)

          cGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATa                                                                                                                             >  1:3931613/1‑140 (MQ=255)

          cGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATa                                                                                                                             >  2:2350986/1‑140 (MQ=255)

          cGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATa                                                                                                                             >  2:4600529/1‑140 (MQ=255)

          cGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATa                                                                                                                             <  1:629891/140‑1 (MQ=255)

          cGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATa                                                                                                                             <  1:629874/140‑1 (MQ=255)

          cGCCCAGCCCGGTCAGAACCCCTGCGCGTCGACCGGAAGCCAGGCGGGCTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGAATGTAAAGGATCCATa                                                                                                                             >  2:3840840/1‑140 (MQ=255)

               aGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAATAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGAt                                                                                                                        >  1:4444015/1‑140 (MQ=255)

                gCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTGAAAAAAGTAATCACGAACAGTCCTACGGGCAGGTAAACGGCATGTAAAGGATCCATAGTGAtt                                                                                                                       >  2:2926644/1‑140 (MQ=255)

                    ggTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCAt                                                                                                                   <  1:661567/140‑1 (MQ=255)

                     gTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATGCATAGTGATTTCATc                                                                                                                  >  1:562756/1‑140 (MQ=255)

                     gTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATc                                                                                                                  >  1:4210810/1‑140 (MQ=255)

                     gTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATc                                                                                                                  >  1:4210145/1‑140 (MQ=255)

                     gTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATc                                                                                                                  >  1:978790/1‑140 (MQ=255)

                     gTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATc                                                                                                                  >  1:1251775/1‑140 (MQ=255)

                         gCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCata                                                                                                              <  2:1486661/140‑1 (MQ=255)

                         gCACCCCTGCCCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATGAACAAAAGTAATCCCGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCAATCGTGATTTCATCCata                                                                                                              <  2:2647976/140‑1 (MQ=255)

                          cACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCataa                                                                                                             >  2:4358863/1‑140 (MQ=255)

                               cTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAg                                                                                                        <  1:1089464/140‑1 (MQ=255)

                               cTGCGCGCCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGCTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATCGTGATTTCATCCATAAATAAg                                                                                                        <  1:1271922/140‑1 (MQ=255)

                                tGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGt                                                                                                       >  1:2589432/1‑140 (MQ=255)

                                tGCGCGTCGACCGAAAGCCAGGCTGTTTTGTACTAACACAAAGTGATTGGCTCCCGTATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGTTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGt                                                                                                       <  2:3757358/140‑1 (MQ=255)

                                  cgcgTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGa                                                                                                     >  2:4134868/1‑140 (MQ=255)

                                    cgTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaac                                                                                                   >  2:2313609/1‑140 (MQ=255)

                                       cGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaactaa                                                                                                >  1:2403246/1‑140 (MQ=255)

                                         aCCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGaactaact                                                                                              >  2:2106766/1‑140 (MQ=255)

                                          ccGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTg                                                                                             <  1:578217/140‑1 (MQ=255)

                                               aGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGcatca                                                                                        >  1:5105821/1‑140 (MQ=255)

                                                   aGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATAtt                                                                                    >  2:2796076/1‑140 (MQ=255)

                                                    ggCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATAttt                                                                                   >  1:859836/1‑140 (MQ=255)

                                                          tttGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCa                                                                             >  1:617777/1‑140 (MQ=255)

                                                                  acCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAaataat                                                                     <  1:5006763/140‑1 (MQ=255)

                                                                   ccACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTTGATCGTATTTCTACCAAAAATAATc                                                                    >  1:2899577/1‑140 (MQ=255)

                                                                   ccACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAACATAATc                                                                    >  2:353512/1‑140 (MQ=255)

                                                                   ccACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATc                                                                    >  1:2638851/1‑140 (MQ=255)

                                                                   ccACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATc                                                                    >  2:255538/1‑140 (MQ=255)

                                                                   ccACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATc                                                                    >  2:255518/1‑140 (MQ=255)

                                                                   ccACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATc                                                                    >  1:2539314/1‑140 (MQ=255)

                                                                   ccACAAAGAGATTGGCTCCCGGAGTAAAAAAAATAATCACGAACAGTCCTGCGGTCAGGTAAACGGCATGTACAGGATCCTTAGTGATGTCATCCATAAATAAGAGAACTAACGACATCATATTTCTAACAAAaataagc                                                                    >  2:5061183/1‑138 (MQ=255)

                                                                          gagaTTGGCTCCCGGTTTAAAAAAAGTAATCCCGAACAGTCCTTCGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGATCTAACTGCATCAGATTTCTACCAAAAATAATCGGGTGCg                                                             <  2:2012637/140‑1 (MQ=255)

                                                                          gagaTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCg                                                             <  1:1540367/140‑1 (MQ=255)

                                                                            gaTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgag                                                           <  1:153046/140‑1 (MQ=255)

                                                                             agtagCTCCCGTATTAAAAAAAGTAAGCACGAACAGTCCTACGGTCAGGTAAAAGGCATGTAAAGGATCCATAGTGATTTCATCCAGACATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGAGTTCgaga                                                          <  2:1013906/136‑1 (MQ=255)

                                                                             aTTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgaga                                                          >  1:5073161/1‑140 (MQ=255)

                                                                             aTTGGCTACCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgagg                                                          >  2:3612056/1‑139 (MQ=255)

                                                                              ttGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAGATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgagag                                                         <  2:1652983/140‑1 (MQ=255)

                                                                              ttGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgagag                                                         <  1:4136379/140‑1 (MQ=255)

                                                                              ttGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgagag                                                         <  1:4600529/140‑1 (MQ=255)

                                                                               tGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCgagaga                                                        <  1:2162456/140‑1 (MQ=255)

                                                                                    cccGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATca                                                   >  1:5216989/1‑140 (MQ=255)

                                                                                    cccGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATca                                                   >  1:5216927/1‑140 (MQ=255)

                                                                                      cggaagaaaaaaaGTAATCACGAACAGACCTACGGTCAGGTAAACTGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATAGGGTGCGAGAGAGATcaca                                                 <  2:4918051/134‑1 (MQ=255)

                                                                                      cGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATcaca                                                 <  1:536018/140‑1 (MQ=255)

                                                                                      cGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATcaca                                                 <  1:5346256/140‑1 (MQ=255)

                                                                                       ggATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACaa                                                <  2:1894287/140‑1 (MQ=255)

                                                                                       ggATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACaa                                                <  2:1894785/140‑1 (MQ=255)

                                                                                        gATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACaaa                                               <  1:1794455/140‑1 (MQ=255)

                                                                                        aaTTAAAAAAACTAATCACTAACACTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACaaa                                               <  2:3971109/139‑1 (MQ=255)

                                                                                         aTTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGTTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTCATCTAACTGTATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAg                                              <  2:961871/140‑1 (MQ=255)

                                                                                                            aaCAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACt                           <  2:5307998/140‑1 (MQ=255)

                                                                                                            aaCAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACt                           <  2:3720352/140‑1 (MQ=255)

                                                                                                              cAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTgg                         <  1:2796076/140‑1 (MQ=255)

                                                                                                              cAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTgg                         <  1:746507/140‑1 (MQ=255)

                                                                                                              cAGTCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTgg                         <  2:5191618/140‑1 (MQ=255)

                                                                                                                 tCCTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGt                      >  1:2039384/1‑140 (MQ=255)

                                                                                                                 tCCTACGGTCAGGTAAACGGCATGTAAAGGATCAATAAGGAATTCATCCAGAAAGAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGAAGCTGATTTCCGGTTACTAGCGt                      >  2:456236/1‑140 (MQ=255)

                                                                                                                  ccTACGGTCAGGTAAACGGCTTGTAAAGGATCCATAGTGATTTCATCCATCAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATACTCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGtt                     <  2:1623020/140‑1 (MQ=255)

                                                                                                                  ccTACGGTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGtt                     <  2:70450/140‑1 (MQ=255)

                                                                                                                        gTCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGcc               <  1:677344/140‑1 (MQ=255)

                                                                                                                         tCAGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGccc              >  2:1100429/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATTAGATTTCTACGAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATGTCCGGTTAGTGGCGTTTATGCCctg            >  2:2497986/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGcc               <  1:2378530/137‑1 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg            >  2:4357424/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg            >  2:1739740/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg            >  1:2105586/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg            >  1:1051871/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg            >  2:512785/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg            >  2:769143/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg            >  2:848115/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg            >  2:4020613/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg            >  1:3246157/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg            >  1:3610035/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg            >  1:5169382/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg            >  2:3079136/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg            >  2:1061995/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGATCCATAGTGATTTCACCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg            >  2:313495/1‑140 (MQ=255)

                                                                                                                           aGGTAAACGGCATGTAAAGGAGCCATAGTGATTTCATCCATAAATAAGTGACCTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctg            >  2:2138787/1‑140 (MQ=255)

                                                                                                                             gTAAACGGCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATAGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCctgac          >  1:561714/1‑140 (MQ=255)

                                                                                                                                  cGGCATGTAACGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGAAc     <  2:3454256/140‑1 (MQ=255)

                                                                                                                                   ggCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGAACt    >  1:2525231/1‑140 (MQ=255)

                                                                                                                                   ggCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGAACt    <  2:4210145/140‑1 (MQ=255)

                                                                                                                                   ggCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGAACt    <  1:4781678/140‑1 (MQ=255)

                                                                                                                                   ggCATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGAACt    <  2:1170952/140‑1 (MQ=255)

                                                                                                                                     cATGTAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGAACTaa  >  1:1016013/1‑140 (MQ=255)

CCCAGCGCCACGCCCAGCCCGGTCAGCACCCCTGCGCGTCGACCGGAAGCCAGGCTGGTTTGTACTACCACAAAGAGATTGGCTCCCGGATTAAAAAAAGTAATCACGAACAGTCCTACGGTCAGGTAAACGGCATGCAAAGGATCCATAGTGATTTCATCCATAAATAAGTGAACTAACTGCATCATATTTCTACCAAAAATAATCGGGTGCGAGAGAGATCACAAAGTGTCTTATTTCCGGTTACTGGCGTTTATGCCCTGACTGAACTAA  >  NZ_CP025268/345201‑345473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`