BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,567,287	T→C	478 (TAA→TAG)	glgA ←	glycogen synthase GlgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,567,287	0	T	C	99.7%	1106.9 / NA	333	478 (TAA→TAG)	glgA	glycogen synthase GlgA
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (169/162); minor base A (1/0); total (171/162)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 336 total aligned reads displayed.

CATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAATTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGAATAGCC  >  NZ_CP025268/3567151‑3567422

cATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATGCATATAGGCGTTTCCTGAAAactat                                                                                                                                      >  1:3834319/1‑140 (MQ=255)

 aTGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAactatt                                                                                                                                     <  2:1332039/140‑1 (MQ=255)

    tttATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCa                                                                                                                                  <  2:4847168/140‑1 (MQ=255)

      tATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAg                                                                                                                                <  1:1989723/140‑1 (MQ=255)

      tATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAg                                                                                                                                <  2:1873257/140‑1 (MQ=255)

      tATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAg                                                                                                                                <  1:1746200/140‑1 (MQ=255)

      tATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAg                                                                                                                                <  2:577920/140‑1 (MQ=255)

             gacgacCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGt                                                                                                                         >  2:4127040/1‑140 (MQ=255)

              acgacCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTa                                                                                                                        <  2:1161551/140‑1 (MQ=255)

                     ggTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCa                                                                                                                 <  1:4225585/140‑1 (MQ=255)

                     ggTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCa                                                                                                                 <  2:1858673/140‑1 (MQ=255)

                                aTCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACtt                                                                                                      >  2:796882/1‑140 (MQ=255)

                                 tCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTc                                                                                                     >  2:599135/1‑140 (MQ=255)

                                   gTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTcgc                                                                                                   <  2:4015906/140‑1 (MQ=255)

                                    tAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTcgcc                                                                                                  <  2:4876475/140‑1 (MQ=255)

                                    tAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTcgcc                                                                                                  >  1:5007592/1‑140 (MQ=255)

                                      aaCATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTcgccgc                                                                                                >  1:3670536/1‑140 (MQ=255)

                                         aTCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGAc                                                                                             <  1:1533619/140‑1 (MQ=255)

                                              cTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCACGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGcc                                                                                        <  2:1802924/140‑1 (MQ=255)

                                              cTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGcc                                                                                        <  1:4401546/140‑1 (MQ=255)

                                              cTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCGAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTGCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGcc                                                                                        <  1:2791085/140‑1 (MQ=255)

                                              cTTGTAAGCGAGAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATACGTACACGGAGCCTCCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACTACTTCGCCGCGACCCGcc                                                                                        >  2:3812824/1‑140 (MQ=255)

                                                    aGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTaa                                                                                  <  1:697332/140‑1 (MQ=255)

                                                        aTAGAGTGCTTAAGAGCTTCTAGGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATc                                                                              >  2:5165800/1‑140 (MQ=255)

                                                        aTAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATc                                                                              >  2:5050680/1‑140 (MQ=255)

                                                          agagTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCa                                                                            <  2:704582/140‑1 (MQ=255)

                                                          agagTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCa                                                                            <  2:352716/140‑1 (MQ=255)

                                                          agagTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCa                                                                            <  1:3944781/140‑1 (MQ=255)

                                                            agagCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTCAACGCAGCATTCATATAGGCGTCTCCTGAAAACGCTTTCAAGCGATAGTAAAACTCACGGTACGACTTGGCCGCGACCTGCCAGCTAAAATCCAtt                                                                          >  2:3657747/4‑140 (MQ=255)

                                                               gCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGcc                                                                       >  2:4415010/1‑140 (MQ=255)

                                                                cTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCa                                                                      <  1:1007304/140‑1 (MQ=255)

                                                                cTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCa                                                                      >  1:1322759/1‑140 (MQ=255)

                                                                cTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCa                                                                      >  1:2290632/1‑140 (MQ=255)

                                                                cTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCa                                                                      <  2:2074828/140‑1 (MQ=255)

                                                                cTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCa                                                                      >  2:2602131/1‑140 (MQ=255)

                                                                   aaGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAg                                                                   >  2:3279760/1‑140 (MQ=255)

                                                                     gagCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGcc                                                                 <  1:3043163/140‑1 (MQ=255)

                                                                        cTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGa                                                              >  1:2862882/1‑140 (MQ=255)

                                                                          tCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACg                                                            <  1:2650259/140‑1 (MQ=255)

                                                                          tCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACg                                                            >  1:588476/1‑140 (MQ=255)

                                                                          tCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACg                                                            >  1:3557129/1‑140 (MQ=255)

                                                                          tCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACg                                                            <  1:3798129/140‑1 (MQ=255)

                                                                               gCTAAGCGTGGGCGATGAATATGTAAACGGAGCATGCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGTCTGACGGTGca                                                       <  2:1381275/140‑1 (MQ=255)

                                                                                 tAAGCGTGGGCGATGAATATGTAAACGGAGCACTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCAAGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGcaca                                                     <  1:4087820/140‑1 (MQ=255)

                                                                                 gaaGGGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCCTTTTCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACGTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCGGACGTTGcaca                                                     <  2:1282657/139‑1 (MQ=255)

                                                                                   aGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTTCACaaa                                                   >  2:282580/1‑140 (MQ=255)

                                                                                   aGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACaaa                                                   >  1:1154164/1‑140 (MQ=255)

                                                                                   aGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACaaa                                                   >  2:4538914/1‑140 (MQ=255)

                                                                                   aGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACaaa                                                   >  1:3266544/1‑140 (MQ=255)

                                                                                     cGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAAcc                                                 >  2:2374816/1‑140 (MQ=255)

                                                                                        gggCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGcc                                              >  2:1414014/1‑140 (MQ=255)

                                                                                        gggCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGcc                                              >  2:1746779/1‑140 (MQ=255)

                                                                                        gggCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGcc                                              >  2:2921616/1‑140 (MQ=255)

                                                                                         ggCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCca                                             <  1:604844/140‑1 (MQ=255)

                                                                                         ggCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCca                                             <  1:1608964/140‑1 (MQ=255)

                                                                                         ggCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCca                                             <  2:1601901/140‑1 (MQ=255)

                                                                                         ggCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCca                                             <  1:4710811/140‑1 (MQ=255)

                                                                                             aTGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGt                                         >  2:3394413/1‑140 (MQ=255)

                                                                                             aTGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGt                                         >  1:5053504/1‑140 (MQ=255)

                                                                                               gAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCAGTAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAAGCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGa                                       <  1:1591469/140‑1 (MQ=255)

                                                                                                   atGTCAACGGAGGCTTCGTCTAGGAGTTTCCCGAAAACTATTTCCAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCAAAAACCGCCACAGTGAAGGa                                   <  1:1351766/140‑1 (MQ=255)

                                                                                                   atGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGa                                   <  2:4658245/140‑1 (MQ=255)

                                                                                                    tGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGAc                                  >  1:5317823/1‑140 (MQ=255)

                                                                                                         aCGGAGCATTCATATAGTCGTTTCCTGAAAACTATTTCAAGCGATAGTAATGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGAc                             <  2:4243422/140‑1 (MQ=255)

                                                                                                         aCGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGAc                             <  1:2434952/140‑1 (MQ=255)

                                                                                                         aCGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGAc                             <  2:1515033/140‑1 (MQ=255)

                                                                                                          cGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGAcc                            >  1:1233327/1‑140 (MQ=255)

                                                                                                          cGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGAcc                            >  1:4038540/1‑140 (MQ=255)

                                                                                                          cGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGAcc                            >  1:1783662/1‑140 (MQ=255)

                                                                                                           ggAGCATTCATATCGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGCCTTCGCCGCGTCCTACCAGCTAACATCCACTGCCATAGCCTGACGTTGCACAGACAGCCACAGGGAAGGACGGGACca                           <  2:4808080/140‑1 (MQ=255)

                                                                                                           ggAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACca                           <  1:1283508/140‑1 (MQ=255)

                                                                                                           ggAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACca                           <  2:5007592/140‑1 (MQ=255)

                                                                                                            gAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACcac                          <  2:2779839/140‑1 (MQ=255)

                                                                                                             aGCATTCATATAGGCGTTTCCTGAAAACTATTTCGAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACcaca                         >  2:4588358/1‑140 (MQ=255)

                                                                                                             aGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACcaca                         >  1:5219508/1‑140 (MQ=255)

                                                                                                             aGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACcaca                         >  1:3117106/1‑140 (MQ=255)

                                                                                                             aGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACcaca                         >  2:4494586/1‑140 (MQ=255)

                                                                                                               cATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCGGCGACCTGCCAGCTAAAATCCAGTGCCATAGCCTGACGTTGCACAAACCGCCACATGGAAGGACGGGACCACAGt                       >  1:3158155/1‑140 (MQ=255)

                                                                                                               cATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGt                       >  1:3475245/1‑140 (MQ=255)

                                                                                                                aTTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTa                      >  2:1072106/1‑140 (MQ=255)

                                                                                                                  tCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGAAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAAACCATTGCCATAGCCTGACGTTGCACAAACCGCCCCAGTGAAGGACGGGACcaatgaaca                    >  2:1929364/1‑133 (MQ=255)

                                                                                                                   cagataGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCCACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAACCCGCCACAGTGAAGGACTGGACCACAGTACaa                   >  2:2168440/4‑140 (MQ=255)

                                                                                                                       taGGCGTTTCCTTAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGAATTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGc               <  2:3069947/140‑1 (MQ=255)

                                                                                                                       taGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCCTTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGc               <  2:1007965/140‑1 (MQ=255)

                                                                                                                       taGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGc               <  1:457551/140‑1 (MQ=255)

                                                                                                                         ggCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCAc             >  1:2361741/1‑140 (MQ=255)

                                                                                                                          gCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACg            <  1:3503350/140‑1 (MQ=255)

                                                                                                                              ttCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCTCAAACCGCCACAGAGAAGGACGGGACCACAGTACAAAAGGACGTCGa        >  2:1711937/1‑140 (MQ=255)

                                                                                                                              ttCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGa        >  1:3069305/1‑140 (MQ=255)

                                                                                                                              ttCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGa        >  1:763862/1‑140 (MQ=255)

                                                                                                                               tCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACCAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGaa       <  1:2076525/140‑1 (MQ=255)

                                                                                                                               tCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGaa       <  1:2921616/140‑1 (MQ=255)

                                                                                                                               tCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGaa       <  1:4518950/140‑1 (MQ=255)

                                                                                                                               tCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGaa       >  2:5341512/1‑140 (MQ=255)

                                                                                                                               tCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGaa       <  1:5058014/140‑1 (MQ=255)

                                                                                                                               tCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGaa       >  1:851494/1‑140 (MQ=255)

                                                                                                                               tCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAAGCCATTGCCAGAGCCTGACGTTGCACAAACCGCCACAGGGAAGGACGGGACCACAGTACAAAAGCACGTCGaa       >  2:4061749/1‑140 (MQ=255)

                                                                                                                               tCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGCTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGaa       <  1:5274044/140‑1 (MQ=255)

                                                                                                                               tCCTAAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGaa       <  2:2881450/140‑1 (MQ=255)

                                                                                                                                ccTGAAAACTATTTCAAGCGATAGTAAAGCTCCCGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATCGCCACAGCCTGACGTTGCACAAACCCCCACACGGAAGGACCCGACCACAGTACAAACGCACGGCGAAt      >  2:385083/1‑140 (MQ=255)

CATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAATTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGAATAGCC  >  NZ_CP025268/3567151‑3567422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`