BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,644,586	A→G	G428G (GGT→GGC)	prlC ←	oligopeptidase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,644,586	0	A	G	98.9%	908.7 / NA	282	G428G (GGT→GGC)	prlC	oligopeptidase A
Reads supporting (aligned to +/- strand): ref base A (2/1); new base G (111/165); total (114/168)
Fisher's exact test for biased strand distribution p-value = 5.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 282 total aligned reads displayed.

CGTGACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCAACAT  >  NZ_CP025268/3644450‑3644722

cGTGACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCt                                                                                                                                       >  1:292571/1‑140 (MQ=255)

 gTGACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTa                                                                                                                                      <  1:1027471/140‑1 (MQ=255)

   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTATCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACg                                                                                                                                    >  1:3903470/1‑140 (MQ=255)

   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTCCAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTaag                                                                                                                                    >  2:5200551/1‑138 (MQ=255)

   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACg                                                                                                                                    <  1:2525340/140‑1 (MQ=255)

   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACg                                                                                                                                    >  1:2114501/1‑140 (MQ=255)

   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACg                                                                                                                                    <  2:3050231/140‑1 (MQ=255)

   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACg                                                                                                                                    >  1:947099/1‑140 (MQ=255)

   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACg                                                                                                                                    >  1:1293939/1‑140 (MQ=255)

    aCCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGc                                                                                                                                   <  2:2020596/140‑1 (MQ=255)

     ccGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTCCCACTTACCGGGCGGTTGAGGTTACAAGTCAAATG‑‑CGCGACCGGTTTTTGCAAAGCACCATCAGCTTTACGCATCTGGCCTAcaca                                                                                                                                  <  2:2245826/140‑1 (MQ=255)

     ccGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCa                                                                                                                                  <  1:3654165/140‑1 (MQ=255)

     ccGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCa                                                                                                                                  <  1:537912/140‑1 (MQ=255)

       gAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGt                                                                                                                                <  2:282790/140‑1 (MQ=255)

        aaCTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTc                                                                                                                               >  2:4249124/1‑140 (MQ=255)

        aaCTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTc                                                                                                                               >  1:5152683/1‑140 (MQ=255)

          caggTGGAAGAGGGTAATCACTTCGTCGTGAGTGAACAGCGCCGGTTTCCCATTTACCGGGAGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTcat                                                                                                                             <  2:3387633/137‑1 (MQ=255)

           tCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTcatc                                                                                                                            <  2:111492/140‑1 (MQ=255)

                     gggTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGcc                                                                                                                  <  1:1734658/140‑1 (MQ=255)

                      ggTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGAGCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGccc                                                                                                                 <  2:4885587/140‑1 (MQ=255)

                        tGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCccg                                                                                                               >  2:4731306/1‑140 (MQ=255)

                        tGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGGTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCccg                                                                                                               >  1:1133530/1‑140 (MQ=255)

                         gATCACTTCGTCGTGAGTCAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAACTCAAATA‑‑CGCGACCGGTTTTTGCAACGAACCATCAGCTTCCCGCATCTCGCCTACGCAGTCATCCATCCACGCCccgc                                                                                                              <  2:412467/140‑1 (MQ=255)

                          aTCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCccgcc                                                                                                             >  1:2001790/1‑140 (MQ=255)

                          aTCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCccgcc                                                                                                             >  1:1830297/1‑140 (MQ=255)

                               ttCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATGCACGCCCCGCCGCGCt                                                                                                        >  2:1252785/1‑140 (MQ=255)

                               ttCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGATGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCt                                                                                                        >  1:751755/1‑140 (MQ=255)

                                    cgtGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGttt                                                                                                   >  2:119665/1‑140 (MQ=255)

                                     gtgcgtcaACAGCGCCAGTTTACCATTTACCGGGCGGTTCCAGTTACACGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCATCTTTACGCATCTGACCTACGCAGTCATCCACCCACGCCCCGCCGCGCTTGtttt                                                                                                  <  2:1049782/140‑1 (MQ=255)

                                     gtgcgcgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGtttt                                                                                                  <  2:2426719/134‑1 (MQ=255)

                                     gtgagtgaCCTGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATT‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCACCCATCCACGCCCCGCCGCGCTTGtttt                                                                                                  <  2:3922953/140‑1 (MQ=255)

                                     gtgagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGGAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGtttt                                                                                                  <  1:4918396/140‑1 (MQ=255)

                                     gtgagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGtttt                                                                                                  <  2:4364257/140‑1 (MQ=255)

                                     gtgagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGtttt                                                                                                  <  2:2461591/140‑1 (MQ=255)

                                     gtgagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGtttt                                                                                                  <  1:3154238/140‑1 (MQ=255)

                                     gtgagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGtttt                                                                                                  <  2:1512491/140‑1 (MQ=255)

                                      tgagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTc                                                                                                 >  1:4190805/1‑140 (MQ=255)

                                       gcgtgaACAGCGCCGGTTTGCCATTTACCAGGCTGTTGAAGTTACAAGTCAAATC‑‑CCCGACCGGTTTTCTCAACGACGCCTCCGCTTTAGGCGTCTGCGCTACGTAGTCCTCCATCCACTCCCAGCCGCGCTTGTTTTCa                                                                                                <  2:2714245/138‑1 (MQ=255)

                                       gagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCACAGATCCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCGCCGCCGCGCTTGTTTTCa                                                                                                <  2:3777239/140‑1 (MQ=255)

                                       gagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGATCCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCa                                                                                                <  1:4252610/140‑1 (MQ=255)

                                       gagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGACCCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCa                                                                                                <  2:128390/140‑1 (MQ=255)

                                       gagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCa                                                                                                <  2:292571/140‑1 (MQ=255)

                                       gagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCa                                                                                                <  2:555746/140‑1 (MQ=255)

                                       gagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCa                                                                                                <  2:3272113/140‑1 (MQ=255)

                                       gagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCa                                                                                                <  2:677763/140‑1 (MQ=255)

                                       gagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCa                                                                                                <  2:2868947/140‑1 (MQ=255)

                                         gtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCa                                                                                                <  2:1636127/140‑1 (MQ=255)

                                         gtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAACGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACg                                                                                              <  2:4771852/140‑1 (MQ=255)

                                         gtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACg                                                                                              <  2:2546318/140‑1 (MQ=255)

                                               aGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATa                                                                                        <  2:2735738/140‑1 (MQ=255)

                                                 cgcCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACa                                                                                      >  1:435807/1‑140 (MQ=255)

                                                    cGGTTTACCATTTCCCGTGCGGGTGAGTTTACACGTACAATA‑‑CCCGACCGGCGTTTGCAAAGAACCATTAGCTTTACGCATCTGGCCTACGCAGGCATCCATCCACGCCCCGCCGCGCGTGTTTTCACGGGCATACAGAt                                                                                   <  2:4540626/140‑1 (MQ=255)

                                                    cGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCTCGCTTGTTTTCACGGGCATACAGAt                                                                                   <  1:2679975/140‑1 (MQ=255)

                                                    cGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAt                                                                                   <  2:3608465/140‑1 (MQ=255)

                                                    cGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAt                                                                                   <  2:592017/140‑1 (MQ=255)

                                                    cGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAt                                                                                   <  1:1521287/140‑1 (MQ=255)

                                                    cGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTGTTTCCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAt                                                                                   <  1:3801182/140‑1 (MQ=255)

                                                     ggTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATc                                                                                  <  1:1201089/140‑1 (MQ=255)

                                                      gTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCg                                                                                 <  1:2733135/140‑1 (MQ=255)

                                                      gTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCg                                                                                 <  2:2501918/140‑1 (MQ=255)

                                                      gTTTACCATTTACCGGGCGGTTGAAGGTACAAGTCAAATA‑‑CGCGGCCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTGTTCACGGGCATACAGATCg                                                                                 >  2:4325632/1‑140 (MQ=255)

                                                          aCCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGt                                                                             <  2:209167/140‑1 (MQ=255)

                                                             aTTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGa                                                                          <  1:3357026/140‑1 (MQ=255)

                                                                     ggCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATGTGGCCTACGCAGTCACCCATACACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACcg                                                                  >  2:951666/1‑140 (MQ=255)

                                                                     ggCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACcg                                                                  >  1:3893306/1‑140 (MQ=255)

                                                                     ggCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACcg                                                                  >  1:3444863/1‑140 (MQ=255)

                                                                     ggCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACcg                                                                  >  2:381261/1‑140 (MQ=255)

                                                                      gCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACcgc                                                                 >  1:140109/1‑140 (MQ=255)

                                                                       cGGTTGAAGTTACAAGTCAAATA‑‑CGCGCCCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACcgcg                                                                <  2:467761/140‑1 (MQ=255)

                                                                              aGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCg                                                         <  1:2990374/140‑1 (MQ=255)

                                                                                  agtaggcAAATA‑‑CGCGATTGGGTTTTGCAACGATCCATCAGACTTACGCATCTTGTCTAAGCAGTCATCCATCCACACCCCCCCGCGCTTGTTCCCACGGGCATACAGATCGAGGTCGAAGCTACCGCGCGGATCGTTAt                                                     <  2:581832/134‑1 (MQ=255)

                                                                                  accaGTCAAATC‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTCCGCAGTCATCCATCCACGCCCCGCCTCGCTTTTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAt                                                     <  2:3534948/137‑1 (MQ=255)

                                                                                  aCAAGTCAACTA‑‑CGCGACCGGTTTTTGCAAAGAAACATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAt                                                     <  2:75212/140‑1 (MQ=255)

                                                                                  aCAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAt                                                     >  2:3163757/1‑140 (MQ=255)

                                                                                  aCAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAt                                                     <  1:5323786/140‑1 (MQ=255)

                                                                                  aCAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAt                                                     <  2:4854257/140‑1 (MQ=255)

                                                                                  aCAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAt                                                     <  1:3787710/140‑1 (MQ=255)

                                                                                       tCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCg                                                <  2:1504395/140‑1 (MQ=255)

                                                                                        cAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGt                                               >  1:4533185/1‑140 (MQ=255)

                                                                                          aaTA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCa                                             >  1:399668/1‑140 (MQ=255)

                                                                                                   gcccGGGTTTTGCAAAGAACCATCAGCTTTACGCAGCTGGCCTCCGCAGTCATCCATCCGCGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGATGCTACCGCGCAGTTCGTTATTTTCGTCATACAGtt                                      <  2:790630/138‑1 (MQ=255)

                                                                                                   gACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGtt                                      >  1:711321/1‑140 (MQ=255)

                                                                                                     ccGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCg                                    <  1:1553024/140‑1 (MQ=255)

                                                                                                        gTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCgaag                                 <  1:4045545/140‑1 (MQ=255)

                                                                                                        gTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCgaag                                 <  2:5094126/140‑1 (MQ=255)

                                                                                                        gTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCgaag                                 <  2:1714094/140‑1 (MQ=255)

                                                                                                         tttttCCAAAGAAACATCAGCCTTACGCATCTGGCCTACGCAGTCATCCAGCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGCTCGAGCTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCgaaga                                <  2:188897/140‑1 (MQ=255)

                                                                                                             tGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACg                            <  1:68521/140‑1 (MQ=255)

                                                                                                                   gAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATc                      >  1:4606778/1‑140 (MQ=255)

                                                                                                                   gAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATc                      >  2:2043920/1‑140 (MQ=255)

                                                                                                                            gCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCa             <  1:4787293/140‑1 (MQ=255)

                                                                                                                             cTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTGCCGCGCAGTTCGTTATTTTCGTCATACAGTTGGAAGAAACGTACATCCGGATGCCAg            >  1:3314238/1‑140 (MQ=255)

                                                                                                                              tttACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGa                  <  1:4687215/133‑1 (MQ=255)

                                                                                                                              tttACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGa           >  1:2362457/1‑140 (MQ=255)

                                                                                                                                tACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACa         >  2:2319662/1‑140 (MQ=255)

                                                                                                                                tACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACa         >  1:2437498/1‑140 (MQ=255)

                                                                                                                                tACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGCTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGACACGTACATCCGGATGCCAGACa         >  2:4188653/1‑140 (MQ=255)

                                                                                                                                tACGCACCTGCCCCACGCAGTCATCCATCCACGCCCCGCCGCCCTTGTGTTCACGGGCATACAGATCGCGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATCCAGTCCGAAGAAACGTACATCCGGATGCCacccc         >  2:3456579/1‑136 (MQ=255)

                                                                                                                                  cgcgTCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATc       <  2:3692553/140‑1 (MQ=255)

                                                                                                                                    cATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCaa     <  1:3500841/140‑1 (MQ=255)

CGTGACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATA‑‑CGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCAACAT  >  NZ_CP025268/3644450‑3644722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`