BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,743,921	T→C	I172I (ATT→ATC)	galK →	galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,743,921	0	T	C	99.7%	947.9 / NA	286	I172I (ATT→ATC)	galK	galactokinase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (136/149); minor base G (1/0); total (137/149)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 286 total aligned reads displayed.

TAAGTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATTATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACGTACC  >  NZ_CP025268/3743785‑3744056

  aGTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGa                                                                                                                                    >  1:3699183/1‑140 (MQ=255)

  aGTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGa                                                                                                                                    >  1:1562756/1‑140 (MQ=255)

  aGTTCTTCCGCTTCACTGGAAGTCGCGGTCGAAACCGTATTGCAGCAGCTGTATGATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGTTCAGGAAGCAGAAAACCAGTTTGTATGCTGTAACTGCGGGATCATGGa                                                                                                                                    >  2:969724/1‑140 (MQ=255)

  aGTTCTTCCGCGTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCGGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGa                                                                                                                                    >  2:3808632/1‑140 (MQ=255)

  aGTTCTTACGCTTCACTGGAAGTCGCGGTAGGAACAGTATGGCAGCAGCTTTATCATCTGGCGCTGGCCGGAGCAGAAATCGGGCTTAACGGTCAGGGAGCGGAAAACACGTTTGTAGGCTGTAACTGCGGGATGATGGa                                                                                                                                    >  2:3384281/1‑140 (MQ=255)

   gTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGAt                                                                                                                                   <  2:707933/140‑1 (MQ=255)

       ttcCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGCTCATGGATCAGc                                                                                                                               >  1:2877338/1‑140 (MQ=255)

       ttcCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGc                                                                                                                               >  2:1845917/1‑140 (MQ=255)

       ttcCGCTTCACTGGAAGTCGCGGTCGGAACCGGATTGCAGCAACCCTATCATCCCGCCCTTGACGCACCAACAATACCGGCAAAAAGTCCGGACGCAGAAAACCGGGTGGTAGCCTGTAATTGCGGAAACATAGATCAGc                                                                                                                               >  2:5095270/1‑140 (MQ=255)

        tcCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCt                                                                                                                              >  2:3269985/1‑140 (MQ=255)

        tcCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCt                                                                                                                              >  1:2009005/1‑140 (MQ=255)

        tcCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCt                                                                                                                              <  1:767317/140‑1 (MQ=255)

              tCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATATGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTc                                                                                                                        >  1:4560505/1‑140 (MQ=255)

                       gTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCgg                                                                                                               >  2:5324437/1‑140 (MQ=255)

                         cgcgGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCa                                                                                                             >  1:3862205/1‑140 (MQ=255)

                           cgGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCaag                                                                                                           <  1:4812872/140‑1 (MQ=255)

                           cgGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCaag                                                                                                           <  1:2401247/140‑1 (MQ=255)

                            ggTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCaaga                                                                                                          <  1:5310056/140‑1 (MQ=255)

                             gTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAACTCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCaagaa                                                                                                         <  1:4642385/140‑1 (MQ=255)

                                ccaaCCCTATTGCCCCAGCTTTATCCTCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAACCCAGTTTGTAGGCTGTAACTGCGGGATCATGCATCAGCTAATTTCCGCGCTCGGCaagaaaga                                                                                                      <  2:1090474/138‑1 (MQ=255)

                                        aTTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACACATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTCTAACTGCGGGAGCATGTATCAGCTAACTTCCGCGCTCGACAAGAAAGTTCATGCCt                                                                                              >  2:5027900/1‑140 (MQ=255)

                                        aTTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCt                                                                                              >  2:2061258/1‑140 (MQ=255)

                                        aTTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCt                                                                                              <  1:5195418/140‑1 (MQ=255)

                                           gcagcaGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGc                                                                                           <  1:4043547/140‑1 (MQ=255)

                                            cagcagCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCt                                                                                          >  2:1550656/1‑140 (MQ=255)

                                            cagcagCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGAGCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCt                                                                                          >  2:2429375/1‑140 (MQ=255)

                                                 gcTTTATCATCTGGCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGTAGCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGGCTTTCTGATCg                                                                                     >  2:741846/1‑140 (MQ=255)

                                                 gcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCg                                                                                     >  2:4903724/1‑140 (MQ=255)

                                                 gcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCg                                                                                     >  2:1437843/1‑140 (MQ=255)

                                                 gcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCg                                                                                     >  2:4734399/1‑140 (MQ=255)

                                                 gcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCg                                                                                     >  1:2312645/1‑140 (MQ=255)

                                                 gcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCg                                                                                     >  1:447909/1‑140 (MQ=255)

                                                 gcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCg                                                                                     >  1:1337781/1‑140 (MQ=255)

                                                 gcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCg                                                                                     >  1:2991276/1‑140 (MQ=255)

                                                 gcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCg                                                                                     >  2:2232216/1‑140 (MQ=255)

                                                 gcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCg                                                                                     >  1:3384889/1‑140 (MQ=255)

                                                 gcTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCg                                                                                     >  1:119194/1‑140 (MQ=255)

                                                  cTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGa                                                                                    <  1:661587/140‑1 (MQ=255)

                                                     tATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTg                                                                                 <  2:4586694/140‑1 (MQ=255)

                                                     tATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTg                                                                                 <  2:3031125/140‑1 (MQ=255)

                                                       tcatcTGCCGATGGACGGCGCACGCATCGCGCTTAACGGTCAGGAAGCAGAAACCCAGTTTCTAGGCTGTAACTGCCGGATCATGGAGCAGCTAAGTCCCGCGCTAGGCAAGAAAGATCCTGCCTTGCTGCCCGCTTGcc                                                                               <  2:1352390/140‑1 (MQ=255)

                                                        catcTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCg                                                                              <  1:2630112/140‑1 (MQ=255)

                                                        catcTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCg                                                                              <  1:1481532/140‑1 (MQ=255)

                                                          tcTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCt                                                                            <  2:2767919/140‑1 (MQ=255)

                                                          tcTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCt                                                                            <  2:3057539/140‑1 (MQ=255)

                                                            tGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCa                                                                          >  1:3516330/1‑140 (MQ=255)

                                                            tGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCa                                                                          >  2:3975221/1‑140 (MQ=255)

                                                            tGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCa                                                                          >  2:3155018/1‑140 (MQ=255)

                                                            tGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCa                                                                          >  1:2065993/1‑140 (MQ=255)

                                                               cgccgaaCGTCGGACAAATCGCCCTTGCCGATCAGGAAGCAGAAAACCAGTTTGTAGCCTGTACCTGCAGGATCGCGGACCAGCTAGGTTCGGCGGGCAGCAAGAAAGATCATGCAGGGCGGCTCCATTGCCGCTCACTg                                                                       <  2:3098165/134‑1 (MQ=255)

                                                               cGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTg                                                                       <  1:4857301/140‑1 (MQ=255)

                                                               cGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTg                                                                       <  2:1698351/140‑1 (MQ=255)

                                                               cGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTg                                                                       <  2:3439562/140‑1 (MQ=255)

                                                               cGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTg                                                                       <  1:3924844/140‑1 (MQ=255)

                                                                 cTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGCGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTggg                                                                     <  1:1767721/140‑1 (MQ=255)

                                                                      cGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCa                                                                <  2:4560505/140‑1 (MQ=255)

                                                                      cGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCa                                                                <  2:2675184/140‑1 (MQ=255)

                                                                        gcgcACAAATCGCGCTTAACGGTCAGGAAGGAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCaaa                                                              >  1:3207828/1‑140 (MQ=255)

                                                                         cgcACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAg                                                             >  2:3854059/1‑140 (MQ=255)

                                                                         cgcACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAg                                                             >  1:1112579/1‑140 (MQ=255)

                                                                         cgcACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAg                                                             >  1:2803981/1‑140 (MQ=255)

                                                                         cgcACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAg                                                             >  1:4336147/1‑140 (MQ=255)

                                                                           cacaAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCCTGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCa                                                           <  2:2576393/140‑1 (MQ=255)

                                                                                 tCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGCTCGATTGCCGCTCACTGGGGACCAAAGCAGTTTcc                                                     <  1:5141971/140‑1 (MQ=255)

                                                                                 tCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTcc                                                     <  2:1976565/140‑1 (MQ=255)

                                                                                 tCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTcc                                                     <  1:3350116/140‑1 (MQ=255)

                                                                                 tCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTcc                                                     <  1:1943050/140‑1 (MQ=255)

                                                                                 tCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTcc                                                     <  1:4772977/140‑1 (MQ=255)

                                                                                 tCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTcc                                                     <  1:556670/140‑1 (MQ=255)

                                                                                 tCGCGCTTAACCGTCAGGAAGAAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCATTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTcc                                                     <  2:662596/140‑1 (MQ=255)

                                                                                    cgcTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATg                                                  >  1:2115814/1‑140 (MQ=255)

                                                                                      cTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGcc                                                >  2:477767/1‑140 (MQ=255)

                                                                                         aaCGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCaa                                             <  1:5107288/140‑1 (MQ=255)

                                                                                              tCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGAACATGGATCAGCTAATTTCTGCGCTGGGCAAGAAACATCATGCCTTGCTGATCGATTGTCGCTCACGGGGTACCAAAGCAGTGTGCATTCCGAAAGgtg                                        >  2:4428724/1‑140 (MQ=255)

                                                                                                 ggAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTgg                                     >  1:777363/1‑140 (MQ=255)

                                                                                                    aGCAGAAAGCCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTg                                  <  1:2262284/140‑1 (MQ=255)

                                                                                                      cAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtc                                >  2:3627418/1‑140 (MQ=255)

                                                                                                       aGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtcg                               <  1:4707790/140‑1 (MQ=255)

                                                                                                       aGAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtcg                               <  2:2713321/140‑1 (MQ=255)

                                                                                                       aGAAAACCAGTCTGTAGGCTGTAACTGCGAGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtcg                               <  2:1148085/140‑1 (MQ=255)

                                                                                                        gAAAACCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtcgt                              >  2:121866/1‑140 (MQ=255)

                                                                                                         aaaaCCAGTTTGTAGGCTGTACCTGAGGGATCATGGATCAGCTACTTACCCCGCTCGGCAAGAAAGATCACGCCTTGCTGATCGATTGACGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTgtcgtc                             <  2:3171325/140‑1 (MQ=255)

                                                                                                          aaaCCAGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGtca                            <  1:26969/140‑1 (MQ=255)

                                                                                                               aGTTTGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGtcatcatc                       <  2:818095/140‑1 (MQ=255)

                                                                                                                 tttGTAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCaa                     >  1:697772/1‑140 (MQ=255)

                                                                                                                     tAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCACAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGt                 <  2:2583545/140‑1 (MQ=255)

                                                                                                                     tAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGt                 <  1:4806777/140‑1 (MQ=255)

                                                                                                                     tAGGCTGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGt                 <  1:3840444/140‑1 (MQ=255)

                                                                                                                          tGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCACGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACtt            >  2:260734/1‑140 (MQ=255)

                                                                                                                          tGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACtt            <  2:4217444/140‑1 (MQ=255)

                                                                                                                          tGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACtt            <  2:1907488/140‑1 (MQ=255)

                                                                                                                          tGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACtt            >  1:2507160/1‑140 (MQ=255)

                                                                                                                          tGTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACtt            <  2:618838/140‑1 (MQ=255)

                                                                                                                          tGTAACTACGGGCTCATGGATCAGCTAATCTCCCCCCTCCGCACGAAAGATCATGCCTTGGTGCGCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCTAAAGGTGTGGCTGTCGTCAGGATCGGAAGTAACtt            >  2:5246977/1‑140 (MQ=255)

                                                                                                                           gtgaCTGCGGGATCATGGATCAGCTACTTTCCGCGCTCGTCAAGAAAGATCATGCCTTGCCTATCGATTGCCGCTCACTGGGGACTAAAGCATTCTCCATGCCCAAAGGCGTGGCTGTCGCCATCATCAACAGTAACtcc           >  2:4636989/4‑138 (MQ=255)

                                                                                                                           gTAACTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTc           >  1:2193096/1‑140 (MQ=255)

                                                                                                                               cTGCGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAAc       <  1:1845917/140‑1 (MQ=255)

                                                                                                                                  cGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACGTa    >  2:2978298/1‑140 (MQ=255)

                                                                                                                                  cGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACGTa    >  1:1000629/1‑140 (MQ=255)

                                                                                                                                  cGGGATCATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACGTa    >  1:1607714/1‑140 (MQ=255)

TAAGTTCTTCCGCTTCACTGGAAGTCGCGGTCGGAACCGTATTGCAGCAGCTTTATCATCTGCCGCTGGACGGCGCACAAATCGCGCTTAACGGTCAGGAAGCAGAAAACCAGTTTGTAGGCTGTAACTGCGGGATTATGGATCAGCTAATTTCCGCGCTCGGCAAGAAAGATCATGCCTTGCTGATCGATTGCCGCTCACTGGGGACCAAAGCAGTTTCCATGCCCAAAGGTGTGGCTGTCGTCATCATCAACAGTAACTTCAAACGTACC  >  NZ_CP025268/3743785‑3744056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`