BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,844,378	T→C	445 (TAA→TAG)	adeQ ←	adenine permease AdeQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,844,378	0	T	C	99.7%	1130.3 / NA	335	445 (TAA→TAG)	adeQ	adenine permease AdeQ
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (121/213); total (122/213)
Fisher's exact test for biased strand distribution p-value = 3.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 336 total aligned reads displayed.

AATCGCTTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCGGTG  >  NZ_CP025268/3844242‑3844510

  tCGCTTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATc                                                                                                                                 <  2:109707/140‑1 (MQ=255)

  tCGCTTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATc                                                                                                                                 >  2:5002028/1‑140 (MQ=255)

    gCTTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAGCCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATcca                                                                                                                               <  2:3893915/140‑1 (MQ=255)

     cTTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATccac                                                                                                                              <  2:4211844/140‑1 (MQ=255)

       tttCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATccacca                                                                                                                            <  1:922934/140‑1 (MQ=255)

            ggCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGaata                                                                                                                       <  2:1487180/140‑1 (MQ=255)

                  ggATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCCGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAACCTTc                                                                                                                 <  2:3269128/140‑1 (MQ=255)

                         aggaCCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCaa                                                                                                          <  2:2340850/137‑1 (MQ=255)

                         aGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCaa                                                                                                          >  2:2097627/1‑140 (MQ=255)

                          gCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCaaa                                                                                                         <  2:1198168/140‑1 (MQ=255)

                          gCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCaaa                                                                                                         >  2:4373173/1‑140 (MQ=255)

                                 aaaaCCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCt                                                                                                  <  2:864011/140‑1 (MQ=255)

                                 aaaaCCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCt                                                                                                  <  1:4687228/140‑1 (MQ=255)

                                 aaaaCCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATCCGTACTCCTGCGTAATCAGAACCCCAGATATCTTGAAACCCTCGCCAGCTACTAATCCACCAGAATAATCTTCATTGCAAACCGAGCt                                                                                                  >  2:694142/1‑140 (MQ=255)

                                 aaaaCCAACCACAAACACAACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCt                                                                                                  <  2:3804073/140‑1 (MQ=255)

                                      caaccaCAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGac                                                                                             <  1:1258544/140‑1 (MQ=255)

                                      caaccaCAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGac                                                                                             <  1:4417326/140‑1 (MQ=255)

                                      caaccaCAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGac                                                                                             <  1:39245/140‑1 (MQ=255)

                                        accaCAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGacca                                                                                           <  2:1049593/140‑1 (MQ=255)

                                        accaCAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGacca                                                                                           <  1:5021676/140‑1 (MQ=255)

                                        accaCAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGacca                                                                                           <  1:896262/140‑1 (MQ=255)

                                        accaCAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGacca                                                                                           <  2:4032706/140‑1 (MQ=255)

                                        accaCAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGacca                                                                                           <  1:3202465/140‑1 (MQ=255)

                                        accaCAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGacca                                                                                           >  1:586229/1‑140 (MQ=255)

                                         ccaCAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGaccac                                                                                          <  2:4939506/140‑1 (MQ=255)

                                         ccaCAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGaccac                                                                                          <  1:2093689/140‑1 (MQ=255)

                                         caacaAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGaccac                                                                                          <  2:2232794/138‑1 (MQ=255)

                                           acaAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGaccacca                                                                                        <  2:2315880/140‑1 (MQ=255)

                                              aaCACTACCGCCAGCGTGGCGAGCATTATCGAGATCCTGAGTTCTCGTGCGTGATCCGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAAGCCACCAGGATAATCTTCAGTGCAAACAGAGCTGCGAccgccacca                                                                                     <  2:4522301/140‑1 (MQ=255)

                                              aaCACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGAccaccacca                                                                                     <  1:3745727/140‑1 (MQ=255)

                                                ctcTACAGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcaca                                                                                   <  2:1583875/138‑1 (MQ=255)

                                                cacTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcaca                                                                                   <  1:4766162/140‑1 (MQ=255)

                                                  cTACCGCCAGTGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                                 <  1:2788129/140‑1 (MQ=255)

                                                  cTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCTTTGCGATCAGGGCTCCGACGAGCTCcacaca                                                                                 <  2:577872/140‑1 (MQ=255)

                                                  cTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAACCAGAGCTGCGACCACCACcacaca                                                                                 <  1:3262993/140‑1 (MQ=255)

                                                  cTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                                 <  2:507127/140‑1 (MQ=255)

                                                  cTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                                 <  1:4845815/140‑1 (MQ=255)

                                                  cTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                                 <  1:1837161/140‑1 (MQ=255)

                                                  cTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                                 <  1:2171821/140‑1 (MQ=255)

                                                   tACCGCCTGCGTGGCGAGCCTTTTCCAGATCATGAGTTCCCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGCATAATCTTCAGTACAAACAGAGCTGCGACCACCACcacacac                                                                                <  2:652032/140‑1 (MQ=255)

                                                   tACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacacac                                                                                <  2:257980/140‑1 (MQ=255)

                                                   tACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacacac                                                                                >  1:352215/1‑140 (MQ=255)

                                                    aCCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacacaca                                                                               >  2:3209678/1‑140 (MQ=255)

                                                    aCCGCCAGCGTGGCGAGAATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTAAGTGCAAACAGAGCTGCGACCACCACcacacaca                                                                               >  2:2003872/1‑140 (MQ=255)

                                                           gCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAg                                                                        >  1:2506034/1‑140 (MQ=255)

                                                               ggCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATcg                                                                    >  1:1171056/1‑140 (MQ=255)

                                                                   aGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGcc                                                                >  1:2892211/1‑140 (MQ=255)

                                                                         aTCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTACTCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                          <  2:2648863/140‑1 (MQ=255)

                                                                         aTCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                          <  1:2327640/140‑1 (MQ=255)

                                                                         aTCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                          <  2:2280391/140‑1 (MQ=255)

                                                                         aTCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                          <  1:3576347/140‑1 (MQ=255)

                                                                         aTCCAGATCATGAGTCCTCATGCGTAATCAGAACTCATGATATCTGGAGACCCTCGCCAGTTACTAATCCACCAGGATACTCTTCAGTGCAAACAGAGCTGCGACCACCACCTCACACAGGTTCAGATCGCGCCAGCGcc                                                          <  2:1330826/140‑1 (MQ=255)

                                                                          tCCAGATCATGCGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGATGCGACCAACACCACACACAAGCTCAGATCGCGCCCgcgcac                                                         >  2:5151751/1‑138 (MQ=255)

                                                                          tCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGccc                                                         <  2:2842591/140‑1 (MQ=255)

                                                                              gATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTg                                                     <  1:1069554/140‑1 (MQ=255)

                                                                              gATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTg                                                     <  2:1444451/140‑1 (MQ=255)

                                                                               aTCATGAGTTCTCCTGCGTAATCAGCACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGc                                                    >  2:927504/1‑140 (MQ=255)

                                                                               aTCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGc                                                    >  1:2043958/1‑140 (MQ=255)

                                                                               aTCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGc                                                    >  1:3377447/1‑140 (MQ=255)

                                                                               aTCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGc                                                    >  2:346316/1‑140 (MQ=255)

                                                                                tCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCa                                                   <  2:410977/140‑1 (MQ=255)

                                                                                   tGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATAc                                                <  1:4925411/140‑1 (MQ=255)

                                                                                      gTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTGAGATCGCGCCAGCGCCCGGTGCATACttt                                             >  2:3749975/1‑140 (MQ=255)

                                                                                        tctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCCGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCa                                           <  1:854755/140‑1 (MQ=255)

                                                                                         ctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                          >  1:1545668/1‑124 (MQ=255)

                                                                                         ctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCat                                          >  2:1279267/1‑140 (MQ=255)

                                                                                         ctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCat                                          >  1:2690313/1‑140 (MQ=255)

                                                                                         ctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCat                                          >  1:884797/1‑140 (MQ=255)

                                                                                         ctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTGCAGATCGCGCCAGCGCCCGGTGCATACTTTCat                                          >  1:2164353/1‑140 (MQ=255)

                                                                                             tGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACGACCACCACCCACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCatgatg                                      <  2:4688249/140‑1 (MQ=255)

                                                                                             tGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCatgatg                                      <  2:2494441/140‑1 (MQ=255)

                                                                                             tGCGTAATCAAAACTCATGATATCTGAAAACCCTCGCCAGTTACTAATCCACCAGAATAATTTTCAGTGCAATCAGGCCACCGCCCACCACCACACACAGGTTCAGATTGCGCCAGCGCCCGGTGCATACTTTCatgatg                                      <  2:4166706/140‑1 (MQ=255)

                                                                                             ggCGTAATCAGAACTCATGAGATATGGAAAACCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGGGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCatgatg                                      <  2:3099399/139‑1 (MQ=255)

                                                                                               cGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCa                                    >  2:810315/1‑140 (MQ=255)

                                                                                                 tAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGt                                  >  1:1082544/1‑140 (MQ=255)

                                                                                                  aaTCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTa                                 <  1:4587759/140‑1 (MQ=255)

                                                                                                  aaTCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTa                                 <  1:1406834/140‑1 (MQ=255)

                                                                                                  aaTCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTa                                 <  2:2169206/140‑1 (MQ=255)

                                                                                                  aaTCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTa                                 <  1:1691873/140‑1 (MQ=255)

                                                                                                      aGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGAc                             >  2:2730932/1‑140 (MQ=255)

                                                                                                      aGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGAc                             >  1:5222274/1‑140 (MQ=255)

                                                                                                             aTGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                          >  1:827828/1‑104 (MQ=255)

                                                                                                              tGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGcc                     <  1:2753501/140‑1 (MQ=255)

                                                                                                              tGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGcc                     <  1:1444726/140‑1 (MQ=255)

                                                                                                               gATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCg                    >  1:3049413/1‑140 (MQ=255)

                                                                                                               gATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCg                    >  2:3697537/1‑140 (MQ=255)

                                                                                                                  atCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGt                 >  2:4736038/1‑140 (MQ=255)

                                                                                                                     tGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCa              <  1:4176339/140‑1 (MQ=255)

                                                                                                                     tGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCa              >  1:741105/1‑140 (MQ=255)

                                                                                                                      ggAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCaa             <  2:4330843/140‑1 (MQ=255)

                                                                                                                      ggAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCaa             <  1:4673467/140‑1 (MQ=255)

                                                                                                                            ccTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTa                                 <  1:2374852/114‑1 (MQ=255)

                                                                                                                            ccTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTa                                 >  2:2374852/1‑114 (MQ=255)

                                                                                                                             cTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTc      >  2:4254491/1‑140 (MQ=255)

                                                                                                                             cTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTc      >  1:1058673/1‑140 (MQ=255)

                                                                                                                              tCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGCGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCg     <  2:5327074/140‑1 (MQ=255)

                                                                                                                              ccgctacttactaGTCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCCCCACCCCACACAGGTTCAGATCTCGCCTGCGCCCGGGGCATACTTTCATGATGCATTACGACATAAAGCCGAGTGCAATCCCTTCg     <  2:2525741/133‑1 (MQ=255)

                                                                                                                               ctccAGTTACTAATCCACCAGAATAATCTTCAGTGCAGACAGAGCTGCGACCACCAGCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTGTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCgg    >  2:2940813/3‑140 (MQ=255)

                                                                                                                               cGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCgg    >  1:4468519/1‑140 (MQ=255)

                                                                                                                               cGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCgg    >  1:4536306/1‑140 (MQ=255)

AATCGCTTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCGGTG  >  NZ_CP025268/3844242‑3844510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`