BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,915,245	A→G	C115R (TGT→CGT)	glmS ←	glutamine‑‑fructose‑6‑phosphate transaminase (isomerizing)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,915,245	0	A	G	99.6%	1735.8 / NA	522	C115R (TGT→CGT)	glmS	glutamine‑‑fructose‑6‑phosphate transaminase (isomerizing)
Reads supporting (aligned to +/- strand): ref base A (0/1); major base G (292/227); minor base T (2/0); total (294/228)
Fisher's exact test for biased strand distribution p-value = 5.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 522 total aligned reads displayed.

CTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACACGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGTGTGAGCAAT  >  NZ_CP025268/3915110‑3915380

cTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCt                                                                                                                                     >  1:993108/1‑140 (MQ=255)

cTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGGTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCt                                                                                                                                     >  1:3002654/1‑140 (MQ=255)

  gTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCttt                                                                                                                                   >  2:2536453/1‑140 (MQ=255)

  gTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCttt                                                                                                                                   >  2:5334162/1‑140 (MQ=255)

      cGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGc                                                                                                                               <  1:2860795/140‑1 (MQ=255)

        tACGCACCACGCAGCTGCGGGATAGCACGCATAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  2:4157024/1‑140 (MQ=255)

        tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  2:4357210/1‑140 (MQ=255)

        tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  2:3938805/1‑140 (MQ=255)

        tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  1:3236705/1‑140 (MQ=255)

        tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  1:2774227/1‑140 (MQ=255)

        tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  1:4463370/1‑140 (MQ=255)

        tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  1:2641687/1‑140 (MQ=255)

        tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  2:2052038/1‑140 (MQ=255)

        tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  1:5261586/1‑140 (MQ=255)

        tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGctc                                                                                                                             >  1:921916/1‑140 (MQ=255)

        tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCCTTTAGctc                                                                                                                             >  2:3586512/1‑140 (MQ=255)

        tACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGGCCCGCCTTGTTTCAGCTCCCAGTTCGCCTGATGGGCAATGCCTGCGAGGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTGTGAGctc                                                                                                                             >  2:4593898/1‑140 (MQ=255)

          cGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCtt                                                                                                                           >  2:746833/1‑140 (MQ=255)

           gCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTc                                                                                                                          >  2:4308364/1‑140 (MQ=255)

           gCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTc                                                                                                                          >  2:4446337/1‑140 (MQ=255)

           gCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTc                                                                                                                          >  2:2471542/1‑140 (MQ=255)

             accacGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCAc                                                                                                                        >  2:337392/1‑140 (MQ=255)

             accacGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCAc                                                                                                                        >  1:1072343/1‑140 (MQ=255)

             accacGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTACCGCCTTGTTGCAGCTGCCAGTTCACCAGATGGGCAATCGCTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTGTTAGCTCTacac                                                                                                                        >  2:3071660/1‑140 (MQ=255)

             accacGCAGCTGCGGGACAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTGCAGAAACGAAGGTAGAGCCACGCGCCTTTAGCTCTTCAc                                                                                                                        >  2:3465934/1‑140 (MQ=255)

               cacGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGc                                                                                                                      >  1:4999510/1‑140 (MQ=255)

               cacGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGc                                                                                                                      >  1:623010/1‑140 (MQ=255)

                acGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCa                                                                                                                     >  1:4341811/1‑140 (MQ=255)

                acGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCa                                                                                                                     <  1:2670567/140‑1 (MQ=255)

                acGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCa                                                                                                                     <  2:2252816/140‑1 (MQ=255)

                acGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCGTTTAGCTCTTCACGcg                                                                                                                     >  1:5071756/1‑139 (MQ=255)

                      cTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGtt                                                                                                               <  1:3675068/140‑1 (MQ=255)

                       tGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTc                                                                                                              >  1:1224952/1‑140 (MQ=255)

                        gCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCa                                                                                                             <  2:3508931/140‑1 (MQ=255)

                           ggATACCAGGCCGAACGGCCTCACGCAGAGCCCCGCCTTGTTTCAGCTCCCAGTGCACCAGATGAGCAATCACTTCGGTGTCGGTTTCAGACACGCAGGTATACCCACTCGCTCTTAGCTCTTCACGCAGCGGGTCATgg                                                                                                          >  2:3783680/1‑140 (MQ=255)

                                gCACGCAGAACGGCCTCACGCAGAGTCCCGTCTTGTTTCAGCTACCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTc                                                                                                     >  2:4893166/1‑140 (MQ=255)

                                gCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGGTGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGGTTAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTc                                                                                                     >  2:4990573/1‑140 (MQ=255)

                                gCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTc                                                                                                     >  2:1902771/1‑140 (MQ=255)

                                gCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTc                                                                                                     >  1:1009203/1‑140 (MQ=255)

                                gCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCGTCACGCAGCGGTTCATGGTTTTc                                                                                                     >  2:1676328/1‑140 (MQ=255)

                                  aagCAGAAAGACCCTACGCAGAGCGCCGCCCTGCTTCAGCTCCCAGTTCACCAGATCAGCAATAACTTCGGTGTAGGTCTCAGAAACAAAGGTATAGCCACGCGCTTTTCGCTCTTCACGCAGCGGTTCATGGTTTTCga                                                                                                   <  2:2625567/138‑1 (MQ=255)

                                     cAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCgatga                                                                                                <  2:678769/140‑1 (MQ=255)

                                     cAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCgatga                                                                                                <  2:952492/140‑1 (MQ=255)

                                     cAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCgatga                                                                                                <  1:3988148/140‑1 (MQ=255)

                                      aGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCgatgat                                                                                               >  1:714716/1‑140 (MQ=255)

                                      aGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGGTCATGGTTTTCgatgat                                                                                               >  1:517866/1‑140 (MQ=255)

                                       gAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGatgatg                                                                                              <  1:3899390/140‑1 (MQ=255)

                                       gAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGatgatg                                                                                              <  1:3450938/140‑1 (MQ=255)

                                       gAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTGCACGCAGCGGTTCATGGTTTTCGatgatg                                                                                              <  2:1080499/140‑1 (MQ=255)

                                           ggCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGt                                                                                          >  1:1332912/1‑140 (MQ=255)

                                             ccTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTa                                                                                        <  1:1438501/140‑1 (MQ=255)

                                               tCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATg                                                                                      <  2:5163255/140‑1 (MQ=255)

                                                 aCGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGca                                                                                    <  1:3746181/140‑1 (MQ=255)

                                                 aCGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGca                                                                                    <  2:1616532/140‑1 (MQ=255)

                                                  cGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGcac                                                                                   <  1:1547699/140‑1 (MQ=255)

                                                   gCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGcacc                                                                                  <  1:4820065/140‑1 (MQ=255)

                                                       agTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGATTCATGGTTTTCGATGATGCCGTTATGcaccacca                                                                              >  2:3641371/1‑140 (MQ=255)

                                                         tCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACcaca                                                                            <  2:4259187/140‑1 (MQ=255)

                                                         tCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACcaca                                                                            <  1:332156/140‑1 (MQ=255)

                                                         tCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCACCGGCTCATGGTTTTCGATGATGCCGTTATGCACCACcaca                                                                            >  2:3319893/1‑140 (MQ=255)

                                                         tCCCGACTCGTTTCAGCTCCCAGTCCACCAGATGGGCAATCCCTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCCCGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACTACcaca                                                                            <  2:3267281/140‑1 (MQ=255)

                                                           ccGCCTTGTTTCAGCTCCCAGTTCTCCAGATGGGCAATCACTTCGGCGTCGGTTTCCGAAACGAAGGTATAGCCACGCGCTTTTAGCCCTTCACGCAGCGGTTCATGGTTGTCGAGGATGCCCTTATGCACCACGACAAt                                                                          >  1:4505381/1‑140 (MQ=255)

                                                           ccGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAAt                                                                          <  1:4633379/140‑1 (MQ=255)

                                                           ccGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAAt                                                                          <  2:1338031/140‑1 (MQ=255)

                                                                 tGTTTCCGCTCCCAGTCCACCAGATGGGCAAACACGTCGGTGCCGGTTTCAGAAACGAAGGTATACCCACGCGCCATTAGCTCTCCACGCAGCGGTGCATGGGTTTCGACAATGCCGTTATGCACCACCACAATCTGATc                                                                    >  2:5104086/1‑140 (MQ=255)

                                                                 tGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTc                                                                    <  1:473813/140‑1 (MQ=255)

                                                                 tGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGGTCATGGTTTTCGATGATGCCGTTATGCACCACCACACTGTGTTc                                                                    >  2:4772410/1‑140 (MQ=255)

                                                                     tCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGaa                                                                <  2:196782/140‑1 (MQ=255)

                                                                                ttCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATg                                                     >  1:68001/1‑140 (MQ=255)

                                                                                     cAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGGCACGCGCTTTTAGCTCTTCACGCAGCGGTTAATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCAt                                                >  2:4722419/1‑140 (MQ=255)

                                                                                      aGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCAtt                                               <  1:5334162/140‑1 (MQ=255)

                                                                                      aGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCAtt                                               <  1:4128798/140‑1 (MQ=255)

                                                                                      aGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCAtt                                               <  1:3491229/140‑1 (MQ=255)

                                                                                        aTGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGatgatg                                                                                              >  1:1135805/1‑91 (MQ=255)

                                                                                        aTGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGatgatg                                                                                              <  2:1135461/91‑1 (MQ=255)

                                                                                        aTGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCa                                             <  1:1980376/140‑1 (MQ=255)

                                                                                            gCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTc                                         >  2:2742299/1‑140 (MQ=255)

                                                                                              aaTCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTg                                       <  1:171351/140‑1 (MQ=255)

                                                                                                   cTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGt                                  <  1:4286707/140‑1 (MQ=255)

                                                                                                   cTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGt                                  <  1:2261910/140‑1 (MQ=255)

                                                                                                   cTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGt                                  <  1:1844823/140‑1 (MQ=255)

                                                                                                     tCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTCCATGGTTTTCGATGATGCCGTTCTGCACCACCACAATGTGTTCAGAAACATGCGGACGCGCATTCACTTCTGAAGGTTc                                >  2:151991/1‑140 (MQ=255)

                                                                                                     tCGGTGGCGGTTTCAGAAACGAAGGTAGAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTGTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTc                                >  2:1824515/1‑140 (MQ=255)

                                                                                                      cGGTGTCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCa                               >  1:538667/1‑140 (MQ=255)

                                                                                                           tCGGTTTCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTg                          >  1:814240/1‑140 (MQ=255)

                                                                                                              gTTTCAGAAACGAAGGTATAGCCACACGCTTTTCGCTCTTCACGCAGCGGTTCATGGTTTTAGATGAGGCCGTTATGCAACGCCACAATCTGTTCAGAAACATGCGGATGCGCATTCACTTCTGTAGGTTCACCGTGGGt                       <  2:2350689/140‑1 (MQ=255)

                                                                                                                ttCAGAAACGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCg                     >  2:2875297/1‑140 (MQ=255)

                                                                                                                     aaaCGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAg                <  2:834351/140‑1 (MQ=255)

                                                                                                                     aaaCGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAg                <  1:2509628/140‑1 (MQ=255)

                                                                                                                     aaaCGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAg                <  1:2120746/140‑1 (MQ=255)

                                                                                                                     aaaCGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAg                <  2:3134579/140‑1 (MQ=255)

                                                                                                                      aaCGAAGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGc               <  1:2900740/140‑1 (MQ=255)

                                                                                                                      aaCGAAGGTAGAGGCACGCGCTTTTCGCTCTTGGCGCAGCGGTTTATGGTGTTCGATGATGCCGTTATGCACCTCCACAATGTGTCCAGAAGCCTGCGGATGCGCATTCGCTTCTGAAGGTTCACCGTGGGTCGCCCAgg               <  2:2927176/140‑2 (MQ=255)

                                                                                                                          aaGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAgt           <  2:633120/140‑1 (MQ=255)

                                                                                                                           aGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAgtg          <  2:1293557/140‑1 (MQ=255)

                                                                                                                           aGGTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAgtg          <  2:4001392/140‑1 (MQ=255)

                                                                                                                             ggataGCCACGCGCTTTTAGGTCTTCGCGCAGCGGTTCATGGTTTTCGATGGTGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGtgtg        <  2:118872/138‑1 (MQ=255)

                                                                                                                             gTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGtgtg        <  1:4417147/140‑1 (MQ=255)

                                                                                                                             gTATAGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGtgtg        <  1:3585233/140‑1 (MQ=255)

                                                                                                                                taGCCACGCGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCCCCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGTGTGAGc     <  2:3872209/140‑1 (MQ=255)

CTGTACCGTACGCACCACGCAGCTGCGGGATAGCACGCAGAACGGCCTCACGCAGAGTCCCGCCTTGTTTCAGCTCCCAGTTCACCAGATGGGCAATCACTTCGGTGTCGGTTTCAGAAACGAAGGTATAGCCACACGCTTTTAGCTCTTCACGCAGCGGTTCATGGTTTTCGATGATGCCGTTATGCACCACCACAATGTGTTCAGAAACATGCGGATGCGCATTCACTTCTGAAGGTTCACCGTGGGTCGCCCAGCGAGTGTGAGCAAT  >  NZ_CP025268/3915110‑3915380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`