BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,958,794	A→G	515 (TAA→TAG)	ilvA →	threonine ammonia‑lyase, biosynthetic

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,958,794	0	A	G	100.0%	766.0 / NA	230	515 (TAA→TAG)	ilvA	threonine ammonia‑lyase, biosynthetic
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (118/112); total (118/112)

Only 100 of 230 total aligned reads displayed.

CGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAAGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTC  >  NZ_CP025268/3958659‑3958929

cGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGATCTTTTTGGCGGGTTAGGGaa                                                                                                                                     <  2:4444103/140‑1 (MQ=255)

 gACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGaaa                                                                                                                                    >  1:1886492/1‑140 (MQ=255)

 gACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGaaa                                                                                                                                    >  1:1944143/1‑140 (MQ=255)

  aCTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGaaaa                                                                                                                                   >  2:1400853/1‑140 (MQ=255)

      cGGGCGCGTACCGGCGGCGTTCGAACTTGGCGACCATGAACCGGATCTCGCAACCCCGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTGTGGCGGGTTAGGGAAAAATGc                                                                                                                               >  2:4115515/1‑140 (MQ=255)

          cgcgTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGa                                                                                                                           <  1:3970890/140‑1 (MQ=255)

          cgcgTACTGGCGGCGTTCGAACTTGGAGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGa                                                                                                                           <  1:1123689/140‑1 (MQ=255)

           gcgTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGAt                                                                                                                          <  1:2138338/140‑1 (MQ=255)

            cgTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATa                                                                                                                         >  2:1606678/1‑140 (MQ=255)

            cgTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATa                                                                                                                         >  1:2754436/1‑140 (MQ=255)

            cgTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATa                                                                                                                         >  2:1469135/1‑140 (MQ=255)

            cgTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATa                                                                                                                         >  1:226161/1‑140 (MQ=255)

            cgTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATa                                                                                                                         >  1:1657346/1‑140 (MQ=255)

               aCTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAgcg                                                                                                                      <  1:3268128/140‑1 (MQ=255)

                    cggcgTTCGTACCTGGCGACCAGGAACCGGATTTCGAACCCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCACTCACCCGGCGTTCAGGTTCTTTTTGGCGGGTCAGGGAAAAATGCCTGATAGcgcttcg                                                                                                                 <  2:733458/140‑1 (MQ=255)

                    cggcgTTCGAACTTGGCGACCATGAACCGGATTTCGAACCCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCTGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGcgcttcg                                                                                                                 <  1:2361003/140‑1 (MQ=255)

                    cggcgTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGcgcttcg                                                                                                                 <  2:3062426/140‑1 (MQ=255)

                    cggcgTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGcgcttcg                                                                                                                 <  1:638663/140‑1 (MQ=255)

                    cggcgTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGcgcttcg                                                                                                                 <  1:5151831/140‑1 (MQ=255)

                    cggcgTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGcgcttcg                                                                                                                 <  1:391127/140‑1 (MQ=255)

                    cggcgTGCGAGCTTGGGGACCATGAGCCGGCTTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATACCCGGGCGTTCAGGTTCTTTTGGGCGGGTTAGGGAACAATGCCTGATAGcgcttcg                                                                                                                 <  2:2601783/140‑1 (MQ=255)

                       cgTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGcgcttcgctt                                                                                                              >  1:1703233/1‑140 (MQ=255)

                         ttCGAACTTGGCGACCCTGAACCGGATTTCGAACCCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATACCCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAACTGCCTGATAGCGCTTCGCTTAt                                                                                                            <  1:2606530/140‑1 (MQ=255)

                         ttCGAACTTGGCGACCATGAAGCGGAGTTCGAAACCCGGCTGGATGAGGTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTTTTTTTGGCGGGTTAGGGAAATATGCCTGATAGCGCTTCGCTTAt                                                                                                            <  2:5125767/140‑1 (MQ=255)

                         ttCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGTCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTAt                                                                                                            <  2:76851/140‑1 (MQ=255)

                         ttCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTAt                                                                                                            >  1:3145517/1‑140 (MQ=255)

                         ttCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTAt                                                                                                            >  2:1034965/1‑140 (MQ=255)

                          tCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATc                                                                                                           <  1:3887020/140‑1 (MQ=255)

                          tCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATc                                                                                                           <  1:4638949/140‑1 (MQ=255)

                             aaCTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAgg                                                                                                        >  2:4052576/1‑140 (MQ=255)

                                 tGGCGACCATGAACCGGATTGCGAAACCCGGCTGAATGAGCTGGGCTACGAGTGCCACGACGAAACCAATGACGCGGGGGTCAGGGTCTTTTTGGCGGGTTAGGGAAAAATGCCTGGTAGCACGTCCCTTATCAGGCCTa                                                                                                    >  2:3821993/1‑140 (MQ=255)

                                        cATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTTGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCcgcgc                                                                                             >  2:3037330/1‑140 (MQ=255)

                                        cATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCcgcgc                                                                                             >  2:2396621/1‑140 (MQ=255)

                                           gAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGAc                                                                                          <  1:4033369/140‑1 (MQ=255)

                                           gAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGAc                                                                                          <  2:5257965/140‑1 (MQ=255)

                                           gAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGAc                                                                                          <  2:3115476/140‑1 (MQ=255)

                                           gAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGAc                                                                                          <  1:497979/140‑1 (MQ=255)

                                              ccGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAAc                                                                                       <  2:1942693/140‑1 (MQ=255)

                                              ccGCATTTCGAAACCCGGCTGAATGAGCTGGGCTACGCTTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACacc                                                                                       <  2:4516476/140‑3 (MQ=255)

                                                 gATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATGGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTc                                                                                    >  2:2706421/1‑140 (MQ=255)

                                                 gATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTc                                                                                    >  1:550148/1‑140 (MQ=255)

                                                  aTTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGTGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCa                                                                                   >  1:2920346/1‑140 (MQ=255)

                                                  aTTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCa                                                                                   >  2:3629815/1‑140 (MQ=255)

                                                  aTTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCa                                                                                   >  1:5904/1‑140 (MQ=255)

                                                  aTTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGATCTTTTTGGCGGGTAAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCa                                                                                   >  2:580452/1‑140 (MQ=255)

                                                      cGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGGTAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTtg                                                                               >  2:1865039/1‑140 (MQ=255)

                                                      cGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTtg                                                                               <  2:1165626/140‑1 (MQ=255)

                                                              ggCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCAGTTGTGGTTCgg                                                                       >  1:2014529/1‑140 (MQ=255)

                                                                gtggcgtaGCTGGCCTACGATTGCCTCTCCGAAATCACTACCCCGGCGTTCCGATTCTTTTTGGCGGGTTAGGGAAAATTCCCTGATAGCGCTTCGCTTATAAGGCCTCCCCGCTCGACGACGTCATTTGTGGTTCGGCa                                                                     <  2:2281335/133‑1 (MQ=255)

                                                                cTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCa                                                                     <  1:841935/140‑1 (MQ=255)

                                                                cTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCa                                                                     <  1:1854788/140‑1 (MQ=255)

                                                                 tGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCaa                                                                    <  1:1786167/140‑1 (MQ=255)

                                                                   aaTGAGCTGGGCTTCGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCaaaa                                                                  <  1:3750385/140‑1 (MQ=255)

                                                                   aaTGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGCCGACGTCATTTGTGGTTCGTCacaa                                                                  >  2:2465051/1‑140 (MQ=255)

                                                                            ggatgcGATTGCCAGGACGAAACCAATAAGCCGGTGTTCTGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCGTCGCTTATAAGGGCTAGCCGCGCGACAACGTCATTTGTGGTTCGGCAAAAGCTTCCGGa                                                         <  2:3549043/135‑1 (MQ=255)

                                                                            ggCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGa                                                         <  2:4378491/140‑1 (MQ=255)

                                                                            ggCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGa                                                         <  2:4135427/140‑1 (MQ=255)

                                                                            ggCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGa                                                         <  1:1080121/140‑1 (MQ=255)

                                                                             gCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGaa                                                        >  1:3178673/1‑140 (MQ=255)

                                                                             gCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGaa                                                        >  1:4798143/1‑140 (MQ=255)

                                                                             gCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGaa                                                        >  1:4399782/1‑140 (MQ=255)

                                                                                 cGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGcc                                                    >  1:3640401/1‑140 (MQ=255)

                                                                                      gCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAAt                                               >  1:997099/1‑140 (MQ=255)

                                                                                      gCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAAt                                               >  1:3310932/1‑140 (MQ=255)

                                                                                      gCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAAt                                               >  2:3236007/1‑140 (MQ=255)

                                                                                      gCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGGTCGGCAAAATCTTCCAGAATGCCTCAAt                                               >  1:2728551/1‑140 (MQ=255)

                                                                                      gCCACGACGAAACCAATAACCCGGCGTGCAGGATCTTCTTGCCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAACCCTACCCGCGCGACAACGTCCTTTGAGGTCCCGCAAAATCCTCCAGCATGCCTCAAt                                               >  2:2259355/1‑140 (MQ=255)

                                                                                       ccACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAAtt                                              >  1:139150/1‑140 (MQ=255)

                                                                                        cACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGGTCGGCAAAATCTTCCAGAATGCCTCAATTa                                             >  1:4210407/1‑140 (MQ=255)

                                                                                         acgacgAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAg                                            <  2:4905665/140‑1 (MQ=255)

                                                                                            acgaAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCgg                                         <  1:421882/140‑1 (MQ=255)

                                                                                              gaAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCt                                       >  1:5091468/1‑140 (MQ=255)

                                                                                                 aCCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCAt                                    >  1:963775/1‑140 (MQ=255)

                                                                                                      tAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGc                               >  2:5020602/1‑140 (MQ=255)

                                                                                                      tAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCGCATGTAGc                               >  2:1202939/1‑140 (MQ=255)

                                                                                                           cGGCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCtt                          <  2:2102006/140‑1 (MQ=255)

                                                                                                            ggCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCttt                         >  1:1952863/1‑140 (MQ=255)

                                                                                                             gCGTTCAGGTTCTTTTTGGCGGGTTGGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCAGCtttt                        >  2:349243/1‑140 (MQ=255)

                                                                                                             gCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCtttt                        >  1:5014199/1‑140 (MQ=255)

                                                                                                             gCGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCtttt                        >  2:4022896/1‑140 (MQ=255)

                                                                                                              cGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAATTGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCttttt                       <  2:4662639/140‑1 (MQ=255)

                                                                                                              cGTTCAGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCttttt                       >  1:825299/1‑140 (MQ=255)

                                                                                                                   aGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTgcg                  <  2:1944156/140‑1 (MQ=255)

                                                                                                                   aGGTTCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTgcg                  <  2:3036084/140‑1 (MQ=255)

                                                                                                                       tCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGcaca              <  1:3844844/140‑1 (MQ=255)

                                                                                                                       tCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGcaca              <  1:1523728/140‑1 (MQ=255)

                                                                                                                       tCTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGcaca              <  2:2774820/140‑1 (MQ=255)

                                                                                                                       tCTTTTCGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGCCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAAGTCGCGGCTCATGTAGCCGCTTTTTCTGCGcacc              <  2:1688655/140‑2 (MQ=255)

                                                                                                                        cTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCGATTAGCGGCTCATGTAGCCGCTTTTTCTGCGcacac             >  1:5278758/1‑140 (MQ=255)

                                                                                                                        cTTTTTGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGcacac             <  2:420509/140‑1 (MQ=255)

                                                                                                                          ttttGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCGAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCacacac           >  1:4458660/1‑140 (MQ=255)

                                                                                                                             tGGCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGcc        <  2:1657346/140‑1 (MQ=255)

                                                                                                                              ggCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGccc       <  2:2745255/140‑1 (MQ=255)

                                                                                                                              ggCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGccc       <  2:4757437/140‑1 (MQ=255)

                                                                                                                              ggCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGccc       <  1:907206/140‑1 (MQ=255)

                                                                                                                              ggCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGccc       >  1:4923444/1‑140 (MQ=255)

                                                                                                                              ggCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGccc       <  1:1256745/140‑1 (MQ=255)

                                                                                                                               gCGGGTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAAGCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCa      >  1:4270138/1‑140 (MQ=255)

                                                                                                                                cGGGTTAGGGAAAAATGCCTGATAGCGCTTGGCTTATCTGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCGCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACATGCCCAg     >  2:338602/1‑140 (MQ=255)

                                                                                                                                   gTTAGGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTc  >  2:4725437/1‑140 (MQ=255)

CGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTAAGGAAAAATGCCTGATAGCGCTTCGCTTATCAGGCCTACCCGCGCGACAACGTCATTTGTGGTTCGGCAAAATCTTCCAGAATGCCTCAATTAGCGGCTCATGTAGCCGCTTTTTCTGCGCACACACGCCCAGCTC  >  NZ_CP025268/3958659‑3958929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`