BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,026,258	A→G	261 (TAA→TAG) L162L (CTT→CTC)	tatD → rfaH ←	3'‑5' ssDNA/RNA exonuclease TatD transcription/translation regulatory transformer protein RfaH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,026,258	0	A	G	99.7%	1100.2 / NA	325	261 (TAA→TAG) L162L (CTT→CTC)	tatD rfaH	3'‑5' ssDNA/RNA exonuclease TatD transcription/translation regulatory transformer protein RfaH
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (109/215); minor base T (0/1); total (109/216)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 325 total aligned reads displayed.

TCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCGGTAATA  >  NZ_CP025268/4026124‑4026393

tcatcCCGCCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAACATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGttt                                                                                                                                    >  1:5004684/1‑140 (MQ=255)

   tcCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCg                                                                                                                                 <  1:3143499/140‑1 (MQ=255)

     ccGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGa                                                                                                                               <  2:2679467/140‑1 (MQ=255)

     ccGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGa                                                                                                                               <  1:3593617/140‑1 (MQ=255)

          gcAAGGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCg                                                                                                                          <  2:1477473/140‑1 (MQ=255)

          gcAACGAGCCAGCCCATCTGCCCCATATTTTGCAGCGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCg                                                                                                                          <  1:1916450/140‑1 (MQ=255)

            aaCGGGCCATCCCATCTGCACCATGTTTGGCAACGGATCGGGCACTGGCGTGGATAAGATGCCGCGGGGCGGGCTGCAGCCACGGATGCTAATGTCAAAACACTGTGTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGt                                                                                                                        <  2:2781083/140‑1 (MQ=255)

            aaCGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGt                                                                                                                        <  2:3242839/140‑1 (MQ=255)

            aaCGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGt                                                                                                                        <  1:1667762/140‑1 (MQ=255)

            aaCGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGt                                                                                                                        <  2:1616106/140‑1 (MQ=255)

            aaCGAGCCAGCCCATCTGCCCCATATTTGGCAACGTATTGCGCACTGCCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGGTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGt                                                                                                                        <  1:133434/140‑1 (MQ=255)

            aaCGAGCCAGCCCACCTGCCCCGTATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACCGATGCTAATGTCAAAACACTGTTTGGTATTGCGTTTTAGAGTTTGCGGAACTCGGt                                                                                                                        <  2:4912379/140‑1 (MQ=255)

                 gccagccTATCTGCCCCATATTTTGCCCCGTATTGCGTACTGGCGTGGAGAAGATGCCGCATGGTTGGATGCGTACACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTAttct                                                                                                                   <  2:1652266/140‑1 (MQ=255)

                 gccagccCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTAttct                                                                                                                   <  1:1105437/140‑1 (MQ=255)

                    agccCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTca                                                                                                                <  1:3078937/140‑1 (MQ=255)

                      cccATCTGCCCCATATTTTGCATCGTATTGCGCACTGGCGTGGAGCAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGACCTCGGTATTCTTcaca                                                                                                              <  2:398529/140‑1 (MQ=255)

                      cccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTcaca                                                                                                              <  1:398341/140‑1 (MQ=255)

                      cccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTcaca                                                                                                              <  1:4232611/140‑1 (MQ=255)

                      cccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTcaca                                                                                                              <  1:339606/140‑1 (MQ=255)

                      cccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTcaca                                                                                                              <  2:5319656/140‑1 (MQ=255)

                      cccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTcaca                                                                                                              <  1:2520843/140‑1 (MQ=255)

                      cccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTcaca                                                                                                              <  1:4482319/140‑1 (MQ=255)

                      cccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTcaca                                                                                                              <  1:1993761/140‑1 (MQ=255)

                      cccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTcaca                                                                                                              <  2:4728313/140‑1 (MQ=255)

                      cccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTcaca                                                                                                              <  1:1229891/140‑1 (MQ=255)

                      cccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGAGGCTAATGTCAAAACACTGTTTGGCATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTcaca                                                                                                              >  2:4947057/1‑140 (MQ=255)

                      cacATCTGCACCATATTTTGCAACGTATTGCTCGCTGGCGTGGAGAAGATGCCGCGTGGCTGGCTGCCACCAGGGATGGTAATGTCAAAACACTGTTTGGGATTGCTCTTTAGAGTTTGCGGAAATCGGTATTCTTcaca                                                                                                              >  2:1206822/3‑140 (MQ=255)

                       ccATCTGCCCCGTATTTTGCAACGTATTGCGAACTGGAGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTAAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                             <  2:1660614/140‑1 (MQ=255)

                       ccATCTGCCCCATCTTTTGCAACGTATTGCGCACTGGCGTGGAGAAGCTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACAATGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                             <  2:2630178/140‑1 (MQ=255)

                       ccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                             <  1:337341/140‑1 (MQ=255)

                       ccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                             <  1:5241880/140‑1 (MQ=255)

                       ccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                             <  1:5119090/140‑1 (MQ=255)

                       ccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                             <  2:2325847/140‑1 (MQ=255)

                       ccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                             <  1:676020/140‑1 (MQ=255)

                       ccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                             <  1:4741772/140‑1 (MQ=255)

                       ccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                             <  1:4669380/140‑1 (MQ=255)

                       ccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                             <  1:1045690/140‑1 (MQ=255)

                       ccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                             <  1:1564784/140‑1 (MQ=255)

                       ccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                             <  2:1541014/140‑1 (MQ=255)

                       ccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                             <  2:1391407/140‑1 (MQ=255)

                       ccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                             <  2:3200201/140‑1 (MQ=255)

                        cATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACt                                                                                                            <  1:5042392/140‑1 (MQ=255)

                          tCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGt                                                                                                                        <  1:1428959/126‑1 (MQ=255)

                          tCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACtgt                                                                                                          >  1:4965949/1‑140 (MQ=255)

                          tCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACtgt                                                                                                          >  1:974272/1‑140 (MQ=255)

                           cTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACtgtg                                                                                                         >  2:3666586/1‑140 (MQ=255)

                              ccccATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCtt                                                                                                      <  2:452272/140‑1 (MQ=255)

                               ccccTATTTTGCAACGTGTTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTa                                                                                                     <  2:2184759/140‑1 (MQ=255)

                               cccATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTa                                                                                                     >  1:579195/1‑140 (MQ=255)

                                   tatTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAAtct                                                                                                 <  1:4579627/140‑1 (MQ=255)

                                   tatTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAAtct                                                                                                 <  2:5156702/140‑1 (MQ=255)

                                   tatTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAAtct                                                                                                 >  1:2359907/1‑140 (MQ=255)

                                   tatTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAAtct                                                                                                 >  1:2101071/1‑140 (MQ=255)

                                   tatTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAAtct                                                                                                 <  1:180687/140‑1 (MQ=255)

                                       ttGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCttt                                                                                             <  2:3374154/140‑1 (MQ=255)

                                       ttGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCttt                                                                                             <  1:1958193/140‑1 (MQ=255)

                                            ccGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaa                                                                                        <  2:2492158/139‑1 (MQ=255)

                                            aCGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaa                                                                                        <  1:482177/140‑1 (MQ=255)

                                             cGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCGGGGTGCCACCACGGATGCGAATGTCAAAACACTGTGTGGGATTGCGTTTTAGAGTGTGCGGAACTCGGGATTCTTCACACTGTGCTTAATCGGTTTATtaat                                                                                       >  2:681052/1‑140 (MQ=255)

                                                 ttGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGa                                                                                   <  2:3034131/140‑1 (MQ=255)

                                                 ttGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGa                                                                                   <  2:4574051/140‑1 (MQ=255)

                                                  tGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGAt                                                                                  <  2:2173486/140‑1 (MQ=255)

                                                    cgcACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTa                                                                                <  2:362194/140‑1 (MQ=255)

                                                    cgcACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTa                                                                                <  2:4779471/140‑1 (MQ=255)

                                                      cACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAg                                                                              <  1:4329269/140‑1 (MQ=255)

                                                        cgggggTGGAGAAGATGCCGCATGGGTGGCTGCCACGACGGGTGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACGCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCa                                                                            <  2:2847149/135‑1 (MQ=255)

                                                        cTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCa                                                                            <  1:1477858/140‑1 (MQ=255)

                                                         tGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCg                                                                                                                                 >  2:2367616/1‑86 (MQ=255)

                                                         tGGCGTGGAGAAGATGCCGCAGGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCaa                                                                           >  2:2099226/1‑140 (MQ=255)

                                                           gCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATa                                                                         >  1:2368296/1‑140 (MQ=255)

                                                            cGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAg                                                                        >  1:4116215/1‑140 (MQ=255)

                                                              tGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCa                                                                      <  2:2544944/140‑1 (MQ=255)

                                                              tGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCa                                                                      <  1:2339551/140‑1 (MQ=255)

                                                              tGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCa                                                                      <  2:920912/140‑1 (MQ=255)

                                                                     gaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGt                                                                                                                        >  1:643107/1‑83 (MQ=255)

                                                                     gaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGt                                                                                                                        <  2:643337/83‑1 (MQ=255)

                                                                     gaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGgagcg                                                               >  1:770823/1‑140 (MQ=255)

                                                                      aTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTCTGGGATTTCTTTTTAGAGTTTGCGGAACTCGGTATTCTTCACAGTGTGCTTAATCTCTTTATTAATGAGATTAAGCAATAGCGTGtagcga                                                              >  2:414245/1‑140 (MQ=255)

                                                                          cGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATCAGATTAAGCAATAGCATGGAGCGAGCCt                                                          <  1:87916/140‑1 (MQ=255)

                                                                                    gctgCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGGTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAATATTAAGCAATAGCATGGAGCGAGCCTCACCATCggg                                                >  2:3141207/1‑140 (MQ=255)

                                                                                         caccacGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCgg                                           >  2:88023/1‑140 (MQ=255)

                                                                                              cGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGaaa                                      <  2:827312/140‑1 (MQ=255)

                                                                                                gaggCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAGCTCGGTATTCTTCACACTGTGCTTAAGCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAAt                                    <  1:3787596/137‑1 (MQ=255)

                                                                                                           cAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGc                         >  1:1745347/1‑140 (MQ=255)

                                                                                                              aaCACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCtt                      <  1:1020117/140‑1 (MQ=255)

                                                                                                                   tGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACTCTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAAc                 >  2:4646021/1‑140 (MQ=255)

                                                                                                                   tGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGCTGAAAATGGCCTGAAAGCCTTCGAAc                 >  2:1786070/1‑140 (MQ=255)

                                                                                                                    gTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCGTTCGAAcg                >  1:4596497/1‑140 (MQ=255)

                                                                                                                    gTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAAcg                >  1:1024487/1‑140 (MQ=255)

                                                                                                                    gTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAAcg                >  1:4548723/1‑140 (MQ=255)

                                                                                                                        gggATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGcc            <  2:4841372/140‑1 (MQ=255)

                                                                                                                        gggATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGcc            >  1:2779039/1‑140 (MQ=255)

                                                                                                                          gATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCtt          <  1:789092/140‑1 (MQ=255)

                                                                                                                          gATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCtt          <  2:4034738/140‑1 (MQ=255)

                                                                                                                          gATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCtt          <  2:3856388/140‑1 (MQ=255)

                                                                                                                          gATTGCGTTTTAGAGTTGGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCtt          <  2:4393439/140‑1 (MQ=255)

                                                                                                                               cGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCa                                                                            <  2:1462717/69‑1 (MQ=255)

                                                                                                                               cGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCGGta     >  1:2937522/1‑140 (MQ=255)

                                                                                                                               cGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCGGta     >  2:3436008/1‑140 (MQ=255)

                                                                                                                               cGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCGTCGAACGCGCCTTCGGta     >  1:4513599/1‑140 (MQ=255)

TCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCGGTAATA  >  NZ_CP025268/4026124‑4026393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`