BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,149,401	A→G	114 (TAA→TAG) I280T (ATT→ACT)	frwD → yijO ←	PTS fructose‑like transporter subunit IIB AraC family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,149,401	0	A	G	99.7%	1026.8 / NA	308	114 (TAA→TAG) I280T (ATT→ACT)	frwD yijO	PTS fructose‑like transporter subunit IIB AraC family transcriptional regulator
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (194/112); total (196/112)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 310 total aligned reads displayed.

GCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAATCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCA  >  NZ_CP025268/4149264‑4149536

          aGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGTGGGTAATGAGCGCGGGCCGAACAGGGCTTTCTGCGAGGCTGCAAACCCATCTTATTCTTGCGCAGTCGGTTTTCCTg                                                                                                                             >  2:2679068/1‑140 (MQ=255)

          aGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTGGTCGGTTTTTCTg                                                                                                                             >  1:4699083/1‑140 (MQ=255)

          aGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTg                                                                                                                             >  2:2355539/1‑140 (MQ=255)

          aGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTGTTCTg                                                                                                                             >  1:1130144/1‑140 (MQ=255)

          aGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCCCGGTGCGCACAGTGCGTTCTGCGCCGGAGCAAACCCATCTACTCCTGTAGTAGTCGGGTTTTCTg                                                                                                                             >  2:1148467/1‑140 (MQ=255)

           gCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGt                                                                                                                            <  2:923956/140‑1 (MQ=255)

              aTTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAg                                                                                                                         <  2:2572379/140‑1 (MQ=255)

               tttGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGt                                                                                                                        <  1:1527203/140‑1 (MQ=255)

               tttGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGt                                                                                                                        >  2:3665107/1‑140 (MQ=255)

               tttGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGt                                                                                                                        >  1:4290297/1‑140 (MQ=255)

                ttGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGtt                                                                                                                       <  2:5165004/140‑1 (MQ=255)

                ttGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGtt                                                                                                                       <  1:1269135/140‑1 (MQ=255)

                ttGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGtt                                                                                                                       <  1:3366814/140‑1 (MQ=255)

                  gAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGCGCGCGGTGCGTAATGTGCTTTCTGCGCCGCATCAAACCCATAGTCTTCTGGAGTCGTCGGTTTTTCTGTCAGttag                                                                                                                     <  2:5323197/140‑3 (MQ=255)

                    aCATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCt                                                                                                                   <  1:5349860/140‑1 (MQ=255)

                     cATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCtg                                                                                                                  <  2:2142885/140‑1 (MQ=255)

                        tGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTgg                                                                                                               >  1:2853220/1‑140 (MQ=255)

                         gCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTggg                                                                                                              >  2:1498729/1‑139 (MQ=255)

                         gCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGt                                                                                                              >  1:735568/1‑140 (MQ=255)

                         gCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGt                                                                                                              >  1:4112305/1‑140 (MQ=255)

                              tATGTGCAATGCCGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGc                                                                                                         <  1:2074331/140‑1 (MQ=255)

                              tATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGc                                                                                                         <  1:1350480/140‑1 (MQ=255)

                                tgtgCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCg                                                                                                       >  1:5049301/1‑140 (MQ=255)

                                 gtgCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCgg                                                                                                      <  1:3863653/140‑1 (MQ=255)

                                  tgCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGc                                                                                                     >  2:862396/1‑140 (MQ=255)

                                  tgCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGc                                                                                                     >  1:5260834/1‑140 (MQ=255)

                                    cAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAAGGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGa                                                                                                   <  1:2538640/140‑1 (MQ=255)

                                       tGCAGCATCTACGCATTCCTGCGTTAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGGTGTACGGCCGGCGatat                                                                                                >  2:5342227/1‑140 (MQ=255)

                                       tGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGgtat                                                                                                >  1:1053191/1‑140 (MQ=255)

                                       tGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGgtat                                                                                                >  2:3074752/1‑140 (MQ=255)

                                       tGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGatat                                                                                                >  2:1655138/1‑140 (MQ=255)

                                       tGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGatat                                                                                                >  1:2770910/1‑140 (MQ=255)

                                       tGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGatat                                                                                                >  1:2605015/1‑140 (MQ=255)

                                       tGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGCGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGatat                                                                                                >  1:3094965/1‑140 (MQ=255)

                                       tGCAGCAGCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGatat                                                                                                >  1:3228502/1‑140 (MQ=255)

                                          agcaTCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTcc                                                                                             >  1:66681/1‑140 (MQ=255)

                                          agcaTCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGCAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTcc                                                                                             >  2:5222444/1‑140 (MQ=255)

                                            caTCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGa                                                                                           >  1:4386359/1‑140 (MQ=255)

                                            caTCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTACTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGGGGTACTGCCGGCGATATTCCGa                                                                                           >  2:2493898/1‑140 (MQ=255)

                                                 aCGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCg                                                                                      >  2:3958013/1‑140 (MQ=255)

                                                   gCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAg                                                                                    >  2:589633/1‑140 (MQ=255)

                                                      ttCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGt                                                                                 >  1:2290133/1‑140 (MQ=255)

                                                      ttCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGt                                                                                 >  1:4155882/1‑140 (MQ=255)

                                                       tCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGtt                                                                                >  1:4936821/1‑140 (MQ=255)

                                                        ccTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTc                                                                               >  1:5159099/1‑140 (MQ=255)

                                                        ccTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTc                                                                               >  1:3224516/1‑140 (MQ=255)

                                                        ccTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTc                                                                               >  2:1281220/1‑140 (MQ=255)

                                                        ccTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGATTTCTGCGCCGCAGCAAACCCATCTGATTCTGGAGAAGTCGGTTTTTCTGTCAGTTGGCTGTGGAACTGCCGGGGATATTCCGACGGCGAGTGTTc                                                                               >  2:698166/1‑140 (MQ=255)

                                                         cTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCg                                                                              >  1:4771334/1‑140 (MQ=255)

                                                         cTGCGTGAGACGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTGCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGATGGCGAGCGTTCg                                                                              <  1:2404163/140‑1 (MQ=255)

                                                            cGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGgtg                                                                           >  1:5083111/1‑140 (MQ=255)

                                                            cGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGgtg                                                                           >  2:1983639/1‑140 (MQ=255)

                                                             gTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGgtgt                                                                          <  1:3635969/140‑1 (MQ=255)

                                                                 gCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGtt                                                                                                                       >  2:3530439/1‑91 (MQ=255)

                                                                 gCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGttttt                                                                      >  2:2985354/1‑140 (MQ=255)

                                                                 gCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGttttt                                                                      >  1:1027798/1‑140 (MQ=255)

                                                                 gCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGttttt                                                                      >  2:3483259/1‑140 (MQ=255)

                                                                 gCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCCGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGttttt                                                                      >  1:3806727/1‑140 (MQ=255)

                                                                      aGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGaa                                                                 >  1:1758288/1‑140 (MQ=255)

                                                                          ggTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTTCCGGCGATATTCCGACGGCTAGCGTTCGGTGTTTTTACGAAACAg                                                             >  2:3098283/1‑140 (MQ=255)

                                                                               tGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTCCTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGc                                                        <  1:4078005/140‑1 (MQ=255)

                                                                                gAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTTCCGGCGATATTCCGACGGCGAGCGGTCGGTGTTTTTACGAAACAGACGGCa                                                       <  1:836435/140‑1 (MQ=255)

                                                                                gAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCa                                                       <  1:2166425/140‑1 (MQ=255)

                                                                                gAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCa                                                       <  1:972143/140‑1 (MQ=255)

                                                                                  gcgcGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGa                                                     >  1:3519215/1‑140 (MQ=255)

                                                                                     cgGGGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGATTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATGTTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAagt                                                  >  2:4475734/1‑140 (MQ=255)

                                                                                        tGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAagtagt                                               >  2:2637982/1‑140 (MQ=255)

                                                                                        tGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTCATTCTGGGGTAGTCGGTCTTTCTGTCAGTTGCCTGCGGTACTGCCGGCGATATTCCGACGGCGAGCGGTCGGTGTTTTTACCAAAAAGACGGCAGAagtagt                                               >  2:4833789/1‑140 (MQ=255)

                                                                                          cgcAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTg                                             <  1:4337483/140‑1 (MQ=255)

                                                                                          cgcAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTg                                             >  2:4664988/1‑140 (MQ=255)

                                                                                               aGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTc                                        >  2:2272883/1‑140 (MQ=255)

                                                                                                  gcattCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCGGTGGTACTGCCGTCGATATTCGGACGGCGAGCGGTCGGTGTTTTTACGAAACAGACGGCAGAAGGAGTTGCTGGCGaa                                     >  2:1437247/4‑139 (MQ=255)

                                                                                                  gCTTTCTGGGCCGCAGCAAACCCCCCTTATCGTGGAGCAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCTGGCGAGATTCCGACGGCGACCGTGCGGTGTTGTGAGGAGACGGGCGGCAGAAGGAGTTGCTGTctcc                                     >  2:4226733/1‑137 (MQ=255)

                                                                                                  gCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGAc                                     >  1:2325493/1‑140 (MQ=255)

                                                                                                  gCTTTCTGCGCCGAAGCAAACCCATCTTATTCTGGAGTATTCGGTTTTTAGGTCAATTGCCTGTGGTACTGCCGGCGTTATTCCGTCGGCTAGCGGTCGGTGGTTTTACGAAACAGACGTCAGAAGTAGTTGCTGTCGAc                                     >  2:3193642/1‑140 (MQ=255)

                                                                                                    tttCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGGCGACaa                                   >  1:4465403/1‑140 (MQ=255)

                                                                                                      tCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAg                                 >  1:2386646/1‑140 (MQ=255)

                                                                                                       cTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGc                                >  1:1909644/1‑140 (MQ=255)

                                                                                                         gcgcCGCAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCg                              <  2:4753340/140‑1 (MQ=255)

                                                                                                           gccgcAGCAAACCCATCTTATTCTGGCGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGCTCGGTGTTTTTACGAAACAGACGGCAGAAGCAGTTGCTGTCGACAACGCCGCa                            >  1:882695/1‑140 (MQ=255)

                                                                                                           gccgcAGCAAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCa                            >  1:935036/1‑140 (MQ=255)

                                                                                                           gccgcAGCAAACCCATCTTATTCTGGAGTAGACGGTTTTTCTGTCAGTTGGCTGTGGTACTGCAGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCa                            >  2:2831925/1‑140 (MQ=255)

                                                                                                               cagcaAACCCATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCg                        <  1:267194/140‑1 (MQ=255)

                                                                                                                      cccATCTTATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCa                 <  1:2248235/140‑1 (MQ=255)

                                                                                                                         aTCTTATTCTGGAGTAGTAGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCActt              <  2:373906/140‑1 (MQ=255)

                                                                                                                            ttATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCActtctt           <  2:174519/140‑1 (MQ=255)

                                                                                                                            ttATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCActtctt           <  2:5008653/140‑1 (MQ=255)

                                                                                                                            ttATTCTGGAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCActtctt           <  1:1442884/140‑1 (MQ=255)

                                                                                                                                   ggAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGATCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCtt    <  1:2909927/140‑1 (MQ=255)

                                                                                                                                   ggAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGCAACAGACGGCAGATGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCtt    <  2:1217932/140‑1 (MQ=255)

                                                                                                                                   ggAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCtt    <  2:5352607/140‑1 (MQ=255)

                                                                                                                                   ggAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCtt    <  1:1999932/140‑1 (MQ=255)

                                                                                                                                   ggAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCtt    <  1:186663/140‑1 (MQ=255)

                                                                                                                                   ggAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCtt    <  2:2605015/140‑1 (MQ=255)

                                                                                                                                   ggAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCtt    <  1:5179841/140‑1 (MQ=255)

                                                                                                                                   ggAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCtt    <  1:3996126/140‑1 (MQ=255)

                                                                                                                                    gAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCTACAAAGCCGCAGTCGTGCTCCACCTCTTTTACCTTc   >  2:4332420/1‑140 (MQ=255)

                                                                                                                                    gAGTAGTCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTc   >  2:4565515/1‑140 (MQ=255)

                                                                                                                                    gAGTAGTCGGTTTTCCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTGCGACGGCGAGCGTTCGGTGTTTTTACGAACCAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTc   <  2:4903947/140‑1 (MQ=255)

                                                                                                                                    gAGTAGTCGGTTGTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTc   >  2:5269387/1‑140 (MQ=255)

GCCGGTGCCGAGCGATTTGAACATTGCCGCTATGTGCAATGCAGCATCTACGCATTCCTGCGTGAGCCGCAGCGGGTAATGAGCGCGGTGCGCAAAGTGCTTTCTGCGCCGCAGCAAACCCATCTTATTCTGGAGTAATCGGTTTTTCTGTCAGTTGGCTGTGGTACTGCCGGCGATATTCCGACGGCGAGCGTTCGGTGTTTTTACGAAACAGACGGCAGAAGTAGTTGCTGTCGACAAAGCCGCAGGCGTGCGCCACTTCTTTTACCTTCA  >  NZ_CP025268/4149264‑4149536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`