Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 4,220,737 G→A A74T (GCG→ACG)  aceK → bifunctional isocitrate dehydrogenase kinase/phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252684,220,7370GA99.0% 1413.8 / NA 381A74T (GCG→ACG) aceKbifunctional isocitrate dehydrogenase kinase/phosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (171/206);  minor base C (0/4);  total (171/210)
Fisher's exact test for biased strand distribution p-value = 1.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 381 total aligned reads displayed.

CCGGTGCGCAGCAGCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGA  >  NZ_CP025268/4220603‑4220873
                                                                                                                                      |                                                                                                                                        
  ggTGCGCAGCAGCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGAcgc                                                                                                                                   <  2:3251236/140‑1 (MQ=255)
  ggTGCGCAGCAGCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGAcgc                                                                                                                                   <  1:3616901/140‑1 (MQ=255)
   gTGCGCAGCAGCGTTTCGAACAGGCCCACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGAcgcg                                                                                                                                  <  2:689688/140‑1 (MQ=255)
    tGCGCAGCAGCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCgg                                                                                                                                 <  1:1014571/140‑1 (MQ=255)
    tGCGCAGCAGCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCgg                                                                                                                                 <  1:4944806/140‑1 (MQ=255)
    tGCGCAGCAGCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCgg                                                                                                                                 <  2:1742063/140‑1 (MQ=255)
              cGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAGAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTtact                                                                                                                       <  2:2973707/140‑1 (MQ=255)
              cGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTtact                                                                                                                       <  2:968737/140‑1 (MQ=255)
              cGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTtact                                                                                                                       <  1:1419127/140‑1 (MQ=255)
              cGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTtact                                                                                                                       <  1:1336876/140‑1 (MQ=255)
                tttCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTtactac                                                                                                                     >  1:912700/1‑140 (MQ=255)
                 ttCGAACAGGCCGATTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACg                                                                                                                    <  1:2468503/140‑1 (MQ=255)
                 ttCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACg                                                                                                                    <  2:3737453/140‑1 (MQ=255)
                      aCAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGACATTTTTACTACGTGTTa                                                                                                               <  1:2123696/140‑1 (MQ=255)
                        gggtcGACTGGCATGGTGTCCAGGAGGCGGTGAAAAACCGTGTTCAGCTTTACGATGATCAAGTTGGTCTGGTCGTGGAGCAACTGCGCTGCCTTACTAACGGCCAAAGTACGGACGCGGCAGTTTTACTACGTGTTaag                                                                                                             <  2:2635068/136‑2 (MQ=255)
                        aGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTaaa                                                                                                             <  1:696599/140‑1 (MQ=255)
                        aGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTaaa                                                                                                             <  2:1578404/140‑1 (MQ=255)
                        aGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTaaa                                                                                                             <  2:1847224/140‑1 (MQ=255)
                        aGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTaaa                                                                                                             <  1:1291371/140‑1 (MQ=255)
                          gCCGACTGGCATGCTATCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAaga                                                                                                           >  2:4460800/1‑140 (MQ=255)
                             gACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCa                                                                                                        <  2:4661176/140‑1 (MQ=255)
                              aCTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCAt                                                                                                       <  1:4269568/140‑1 (MQ=255)
                               cTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCAtt                                                                                                      >  2:5094089/1‑140 (MQ=255)
                                    aTGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACAcc                                                                                                 >  1:4806514/1‑140 (MQ=255)
                                    aTGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACAcc                                                                                                 >  2:4845296/1‑140 (MQ=255)
                                         gTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCt                                                                                            <  1:2516022/140‑1 (MQ=255)
                                          tCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTg                                                                                           >  1:1904103/1‑140 (MQ=255)
                                          tCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTg                                                                                           >  2:833193/1‑140 (MQ=255)
                                             agcagGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCAGTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTg                                                                                        >  2:459589/1‑140 (MQ=255)
                                               cagGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGcc                                                                                      >  1:4511064/1‑140 (MQ=255)
                                               cagGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGGCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCGGTTGcc                                                                                      >  1:1105228/1‑140 (MQ=255)
                                                  gCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGTTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGTCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGCGCATGACAGCCGGCTGTTTCCTGa                                                                                   >  2:4903061/1‑140 (MQ=255)
                                                   cGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGAt                                                                                  <  1:3081663/140‑1 (MQ=255)
                                                    gatgccaaaCCGTATCCATCTTTACGTTCATCCCGTTGGTCTGGTCGTGGAGCAACTGCGCCGCCTTACTAACGGCCAACGTACGGCCGCCCCACTTCTACCATGTTTAATATTGCTTCACACCGGCCTGTTGCCGGAtt                                                                                 >  2:4768475/7‑140 (MQ=255)
                                                    gATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGAtt                                                                                 >  1:233807/1‑140 (MQ=255)
                                                    gATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGAtt                                                                                 >  1:2862571/1‑140 (MQ=255)
                                                    gATGAAAAACCGTATCCATCTTTACGATCAGCACGTTGGTCTGGTCGTGGAGAAACTGCGCTGCATTACTAAAGGCCAAAGTACGGACGCGGCATTGTTACTACGTGGTAAAGAGCATTACACCCGGCTGTTGACGGAtg                                                                                 >  2:646872/1‑139 (MQ=255)
                                                       gAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTAcc                                                                              <  2:3583591/140‑1 (MQ=255)
                                                       aaaaaaCCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCAATACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTAcc                                                                              <  1:4723768/139‑1 (MQ=255)
                                                            aCCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCgcgc                                                                         >  2:1464575/1‑140 (MQ=255)
                                                              cgcaTCGATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAACGTCCGGACGAGGCATTTTTACTTCGTGGTAAAGAGCATTACGCCCGGCTGTTGCCGGATTATCCGCGCtt                                                                       <  2:551116/137‑1 (MQ=255)
                                                              cGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCtt                                                                       <  1:3723040/140‑1 (MQ=255)
                                                              cGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCtt                                                                       >  1:5070918/1‑140 (MQ=255)
                                                              cGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCtt                                                                       <  2:3447441/140‑1 (MQ=255)
                                                                tATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCg                                                                     >  2:2251662/1‑140 (MQ=255)
                                                                tATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCg                                                                     <  1:1753515/140‑1 (MQ=255)
                                                                tATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGAGTACCCGCGCTTCg                                                                     >  2:2305952/1‑140 (MQ=255)
                                                                          tACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGga                                                           >  1:1657100/1‑140 (MQ=255)
                                                                            cGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGgaga                                                         <  2:427447/140‑1 (MQ=255)
                                                                              atcatcACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGc                                                       <  2:1666558/140‑1 (MQ=255)
                                                                              atcatcACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGc                                                       <  1:1573030/140‑1 (MQ=255)
                                                                              atcatcACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGc                                                       <  2:2442347/140‑1 (MQ=255)
                                                                                catcaCGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGTCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGCGAGCtt                                                     >  2:4217188/1‑140 (MQ=255)
                                                                                 atcaCGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCttt                                                    >  2:1244920/1‑140 (MQ=255)
                                                                                 atcaCGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAAAGCGTTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCttt                                                    >  1:3816059/1‑140 (MQ=255)
                                                                                     cGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTa                                                <  2:2913755/140‑1 (MQ=255)
                                                                                     cGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTa                                                <  1:4163259/140‑1 (MQ=255)
                                                                                        tggtctggtcGTGGCGCAACTGCGCTGCATTACTAACGGCCAAAGGACGGACGCGGCCTTTTTACTACGTGTTACAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACt                                             <  2:2122305/140‑1 (MQ=255)
                                                                                        tggtctggtcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACt                                             <  1:1214819/140‑1 (MQ=255)
                                                                                         ggtctggtcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTTTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTc                                            >  1:25686/1‑140 (MQ=255)
                                                                                         ggtctggtcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTc                                            >  1:33434/1‑140 (MQ=255)
                                                                                         ggtctggtcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTc                                            >  2:3868635/1‑140 (MQ=255)
                                                                                             tggtcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCgtg                                        >  1:3962096/1‑140 (MQ=255)
                                                                                                tcGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTAc                                     >  2:1966934/1‑140 (MQ=255)
                                                                                                 cGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACt                                    <  1:2924632/140‑1 (MQ=255)
                                                                                                    ggAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTc                                 >  2:1009499/1‑140 (MQ=255)
                                                                                                    ggAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTc                                 <  1:2618553/140‑1 (MQ=255)
                                                                                                    ggAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTc                                 >  1:586954/1‑140 (MQ=255)
                                                                                                    ggAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTc                                 >  1:3762294/1‑140 (MQ=255)
                                                                                                    ggAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTc                                 >  2:3208540/1‑140 (MQ=255)
                                                                                                     gAGCAACTGCGCTGCATTACTGACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGGATTACACCCGGCTGTTGCCGGATTACCCGCGCATCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCg                                >  2:4437646/1‑140 (MQ=255)
                                                                                                     gAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCg                                <  2:1618826/140‑1 (MQ=255)
                                                                                                       gCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGt                              <  2:1302867/140‑1 (MQ=255)
                                                                                                       gCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGt                              <  1:5273326/140‑1 (MQ=255)
                                                                                                         aaCTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCTTGTACTGTCGGTTa                            >  2:1804873/1‑140 (MQ=255)
                                                                                                         aaCTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGt                              <  2:2603974/138‑1 (MQ=255)
                                                                                                          aCTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTAt                           >  2:215346/1‑140 (MQ=255)
                                                                                                          aCTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTAt                           >  1:224911/1‑140 (MQ=255)
                                                                                                          aCTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTAt                           >  1:99074/1‑140 (MQ=255)
                                                                                                              cgcTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGa                       >  2:4979364/1‑140 (MQ=255)
                                                                                                               gcTGCATTACTCACGGCCAAAGTACCGACGCGGCATTTTTACTACGTGTTCAAGAGAATTACACCCGGCTGTGGCCGGATGACCCGCGCTTCGAGACTGCGGAGAGCTTTCTTAGCTCCGTGTACTGTCGGTTATGTGac                      <  2:2932714/140‑1 (MQ=255)
                                                                                                               gcTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGac                      <  2:2758262/140‑1 (MQ=255)
                                                                                                               gcTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGac                      <  1:353454/140‑1 (MQ=255)
                                                                                                                  gCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGaccac                   <  2:3069834/140‑1 (MQ=255)
                                                                                                                   cATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTACAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTATACTGTCGGTTATTTGaccacc                  >  2:4191190/1‑140 (MQ=255)
                                                                                                                        cTAACGGCCAAATTACGGACGCGGCATTTTTACTACGTTTTAAAGAGCAGTACACCCGGCTGTTGCCGTATTACCCGCGCTGCGAGTTGGCGGAGAGCGTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACcgctcg             >  2:1385736/1‑140 (MQ=255)
                                                                                                                        cTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACcgctcg             >  1:1642920/1‑140 (MQ=255)
                                                                                                                        cTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACcgctcg             >  1:3419021/1‑140 (MQ=255)
                                                                                                                          aaCGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACcgctcgct           <  1:107766/140‑1 (MQ=255)
                                                                                                                          aaCGGCCAAAGTACGGACGCGGAATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGTATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACcgctcgct           <  2:365259/140‑1 (MQ=255)
                                                                                                                           aCGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCtt          >  1:744053/1‑140 (MQ=255)
                                                                                                                           aCGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCtt          >  1:204999/1‑140 (MQ=255)
                                                                                                                           aCGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCtt          >  2:3502966/1‑140 (MQ=255)
                                                                                                                             ggCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGCTTGCGGAGAGCTTTTTTAACTCCGTGTACTGTAGGTTATTTGACCACCGCTCGCTTAc        >  2:4831256/1‑140 (MQ=255)
                                                                                                                             ggCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTAc        >  1:4000437/1‑140 (MQ=255)
                                                                                                                             ggCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTAc        >  1:436810/1‑140 (MQ=255)
                                                                                                                             ggCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTAc        >  1:545952/1‑140 (MQ=255)
                                                                                                                             ggCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTAc        >  1:1168823/1‑140 (MQ=255)
                                                                                                                               ccAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACACGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTGGTTTAACGCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTc      >  2:403681/1‑140 (MQ=255)
                                                                                                                                cAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTcc     >  2:775140/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                        
CCGGTGCGCAGCAGCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTGCGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGA  >  NZ_CP025268/4220603‑4220873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑