BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,608,154	G→A	noncoding (48/87 nt)	CXP41_RS23870 ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,608,154	0	G	A	98.0%	789.8 / ‑1.4	250	noncoding (48/87 nt)	CXP41_RS23870	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base G (4/1); new base A (131/111); total (137/113)
Fisher's exact test for biased strand distribution p-value = 3.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

Only 100 of 250 total aligned reads displayed.

CCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGC  >  NZ_CP025268/4608020‑4608290

ccACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGAACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACt                                                                                                                                     <  1:584450/140‑1 (MQ=255)

      ccGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGCACCCCCACGTCCGTAAGAACACTAACAcc                                                                                                                               >  1:995479/1‑140 (MQ=255)

      ccGGAAGGACACTAACACCTGAAGCTAGCGCTTCTACCAATTCCGCAACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACAcc                                                                                                                               <  1:313411/140‑1 (MQ=255)

        gTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGGCTTGAACCCCCACGTCCGTAAGAACACTAACACCTg                                                                                                                             >  1:2155658/1‑140 (MQ=255)

        gTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTg                                                                                                                             >  1:3790231/1‑140 (MQ=255)

        gTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTg                                                                                                                             >  1:3803969/1‑140 (MQ=255)

        gTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGGGCG‑AGGGGGGGGGCTTGAACCCGCTCGTCCGTAAGAACACTAACACCTg                                                                                                                             >  1:876185/1‑140 (MQ=37)

         tAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTAACATTACTTTTATTGATATCCCCCCGCTGAGGGCG‑AGTCGGGTGGCTTGCAGCTCCACGTCCATAGTTACACTAACACCtaa                                                                                                                            >  2:2188324/1‑138 (MQ=9)

              acacTAACACCTGAAGCTAGTGCGTCTACCAATTCCGCGATGTTAGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                                                                                                       <  2:5069635/140‑1 (MQ=37)

              acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTa                                                                                                                       >  1:4185473/1‑140 (MQ=255)

               cacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTAATTGATATCGCCTCGTTTGGTGCG‑ATGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTaa                                                                                                                      >  1:679656/1‑139 (MQ=255)

                acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTaa                                                                                                                                   <  1:524526/126‑1 (MQ=255)

                acTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGCCATCGTACTTTTTTTGATATCGCCTCGTTTGGTGGG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAgg                                                                                                                     >  2:2165601/1‑139 (MQ=255)

                aatatcacCTGAAGCTAGCGCTTCTACCAATTCCGCCACCTTCCCACAGTTGTCGTCCTTATTATGATATCGCTTCCCTTAGAGCG‑AGGGCGCGTGCTTTAAACCCCACGACCTGAAAAACACTAACACCTGAATCTAgc                                                                                                                     >  2:4748370/6‑140 (MQ=12)

                   aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGcgcg                                                                                                                  >  2:2233405/1‑140 (MQ=255)

                   aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGcgcg                                                                                                                  <  1:5004723/140‑1 (MQ=255)

                    acacCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTAATCTTACTTATATTGATATCGCCCCGCTTGGGGCG‑AGGCGGGTGGCTTGCACCCCCTCGTCCATAATAACACTAACACCTGAAGCTAgtgcct                                                                                                                 >  2:3503059/1‑138 (MQ=21)

                        cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGG‑GCGCAGGGGGGGGGCTTTCACCCCCACGTCCGTAATAACATTTAGACCTGGAGCTAGATCGTGTAc                                                                                                             >  2:2335146/1‑140 (MQ=25)

                        cTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCATACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAATAACACAAACACCTGAGGCTAACGCGTCTAc                                                                                                             >  1:4222788/1‑140 (MQ=37)

                          gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGATTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                                                           >  1:585256/1‑140 (MQ=255)

                          gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                                                           >  1:3695160/1‑140 (MQ=255)

                          gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTCCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACAAACACCTGAAGCTAGCGCGTCTACCa                                                                                                           >  1:1810612/1‑140 (MQ=255)

                          gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGGGATCTTACTTGTTTTGATATAGCATCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                                                           <  1:4573561/140‑1 (MQ=21)

                            aGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAAt                                                                                                         >  1:3916935/1‑140 (MQ=255)

                                aGCGCGTCTACCCATTCCGCCACCTTCGCACAGTCACCTTACTTTTTTAGATATCCTCTCTCGGAGGGCG‑CGGAGGGGGGCTTTCACCTCCTCGTCCGTAGGAACACTACCACCGGAAGGTAGCGGGTGTACCAAtgtcc                                                                                                     >  2:5280474/1‑136 (MQ=255)

                                aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGCACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                                                     >  1:4334042/1‑140 (MQ=255)

                                aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGCACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                                                     >  1:3397228/1‑140 (MQ=255)

                                aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                                                     >  1:116246/1‑140 (MQ=255)

                                aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCg                                                                                                     >  2:5299624/1‑140 (MQ=255)

                                aGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCCCCTCGCTTGGTGCG‑AGGGGGGGGACTTGCACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGTGCGTCTACCAATTccc                                                                                                     >  2:4834598/1‑139 (MQ=25)

                                 tcgctTCGACAAATTCCGTCTCCTGCGCTCAGTGAGCTGACGCTTTTGGACATCGCCTCGTGTGGTGCG‑AGGGGGGGAACCTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                                                    <  2:5148710/139‑1 (MQ=9)

                                     gTCAACCAATTCCGCCACCTTCACACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                                                                                                <  1:1090646/140‑1 (MQ=255)

                                      tCTACCAATTCCGCCACCTTCGCACACTCCTCTTTCTTTTTTTGAGATCGCCTCTTTCGGGGCC‑ACGGGGGGGGCTTTAACCCCCACGCCCGTAGCGACATTAACACCTGGAGCTAGCTCGTATACAGATTCCCCCCCCt                                                                                               >  2:1293875/1‑140 (MQ=16)

                                       cTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                                                                                              >  1:3331104/1‑140 (MQ=255)

                                        tACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                                                                                             >  1:3438396/1‑140 (MQ=255)

                                           ccaTTCCGCCACAATCGAACAGTGAACTTACTTTTTATGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                          <  2:767150/138‑1 (MQ=17)

                                           cAATTCCGGCACCTTCACACAGTGATGTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                          <  2:4402005/140‑1 (MQ=18)

                                           cAATTCCGCCACCTACACACAGGCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                          <  2:1112785/140‑1 (MQ=255)

                                           cAAATCCGCCACCATACCACAGGCATCTGACTTTATTTGATATCACCACGGTTGGTGCG‑AGGGGGGGGACGTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                          <  2:2930797/140‑1 (MQ=17)

                                            aaTCCCGCCACCTCAGCACAGTCATCTTACTTTTTTTGATGTCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGccc                                                                                         <  2:374689/140‑1 (MQ=18)

                                                ccGCCCACTTCGCACAGTCATGTTACTATTGTTGGTACCGGCTCGCTTGGTGCG‑AGGGGGGGGCTTTGCCCCCCCACGCCCGTAAGCGCACTACCACCTGAAGCTAGCGCGTCTACCAATCCCGCCACCTTCGCCCAGTg                                                                                     <  2:3135475/140‑1 (MQ=12)

                                                 cGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTACG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGATAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGc                                                                                    >  2:4323407/1‑140 (MQ=255)

                                                  gCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg                                                                                   <  1:197071/140‑1 (MQ=255)

                                                  gCCACCTTCGCACAGTCATCTTACTTTTTTTCATATAGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCg                                                                                   <  1:1364913/140‑1 (MQ=255)

                                                    cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                                                 <  1:3572433/140‑1 (MQ=255)

                                                    cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                                                 >  2:3618253/1‑140 (MQ=255)

                                                    cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                                                 <  1:2919827/140‑1 (MQ=255)

                                                    cACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAg                                                                                 <  1:4524466/140‑1 (MQ=255)

                                                         tCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAata                                                                            >  1:2814747/1‑140 (MQ=255)

                                                              caGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc                                                                       <  1:5299624/140‑1 (MQ=255)

                                                              caGGCATCTTACTTTATTTGATATCTACTCGCTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAAc                                                                       <  2:1011621/140‑1 (MQ=18)

                                                                gTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGt                                                                     >  1:3449687/1‑140 (MQ=255)

                                                                 tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                          <  1:1605360/118‑1 (MQ=255)

                                                                 tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGcc                                                                                          >  2:1605360/1‑118 (MQ=255)

                                                                 tCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGCGCGAGCAATATCAACGTg                                                                    >  2:3675205/1‑140 (MQ=255)

                                                                   aTCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGt                                                                  >  2:175319/1‑140 (MQ=255)

                                                                         cTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTgg                                                            >  1:3635142/1‑140 (MQ=255)

                                                                             ttttGATATCGCCTCGTTTGGTGCG‑GGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCg                                                        <  2:693029/140‑1 (MQ=255)

                                                                             ttttGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCg                                                        <  2:166841/140‑1 (MQ=255)

                                                                              tttGATATCGCCCCGTTTGGAGGG‑AGGGGGGGGACTTGAACCACCACGTGCGTAAGAACACTAACACTTGAAGCTAGCGCGTCTCCCATTTCCGCCACCTTCGCCCAGTACGATCAATATCAACGTGGTTTTTGGTGCGa                                                       <  1:3198820/140‑1 (MQ=18)

                                                                                   tatCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggg                                                  <  2:3790231/140‑1 (MQ=255)

                                                                                             tttGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGAGGTTTTTGGTGCGAGGGGGGGGACTTGag                                        >  1:4731066/1‑139 (MQ=255)

                                                                                                 gTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAcccc                                    <  1:2221364/140‑1 (MQ=255)

                                                                                                 gTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACGAACACCAGAAGCTAGCGCGTCTACCACTTCCGCCACCTTCGACAAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAcccc                                    <  2:3447173/140‑1 (MQ=21)

                                                                                                  tGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAccccc                                   >  1:2801866/1‑140 (MQ=255)

                                                                                                  tGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAccccc                                   <  1:4104829/140‑1 (MQ=255)

                                                                                                  tGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAAccccc                                   >  1:2681960/1‑140 (MQ=255)

                                                                                                   gCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATACCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCa                                  <  2:370814/140‑1 (MQ=255)

                                                                                                   gCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCa                                  <  1:1039455/140‑1 (MQ=255)

                                                                                                     g‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACg                                <  2:605279/140‑1 (MQ=255)

                                                                                                      agggggggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACg                                >  1:4504376/3‑140 (MQ=255)

                                                                                                       aGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCACTATCAACGTGGTGTTTGGTGCGAGGGGCGTGCCTTGAGCCTCCagct                               >  2:3976066/1‑137 (MQ=18)

                                                                                                       aGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGCCTTGAACCCCCACGt                               >  1:5152055/1‑140 (MQ=255)

                                                                                                       aGGGGGGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGCCTTGAACCCCCACGt                               >  1:1749371/1‑140 (MQ=255)

                                                                                                            ggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGTGACTTGAACCCCCACGTCCGTa                          >  1:4867032/1‑140 (MQ=255)

                                                                                                            ggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTa                          <  1:1913753/140‑1 (MQ=255)

                                                                                                            ggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGGTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTa                          >  2:642812/1‑140 (MQ=255)

                                                                                                            ggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTCCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTa                          <  1:2346503/140‑1 (MQ=255)

                                                                                                             gggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTaa                         >  1:1795070/1‑140 (MQ=255)

                                                                                                              ggACTTGACCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTCCCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAg                        <  1:456754/140‑1 (MQ=255)

                                                                                                              ggACTTGAACCCCCACGTCCGTAAGAACACTAACCCCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAg                        <  1:3232783/140‑1 (MQ=255)

                                                                                                              ggACTTAACCCCCCACGTCTTTAAGAACACTAACACCTGAAGCTACCGCAGATTCCAGCTCCGTCACCCACGCACAGTGCGAGCAATTTAAACATGGTATTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAg                        <  2:3862523/140‑1 (MQ=16)

                                                                                                                   tGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGacac                   >  1:4065981/1‑140 (MQ=255)

                                                                                                                    gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                  <  1:642812/140‑1 (MQ=255)

                                                                                                                    gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                  <  1:77908/140‑1 (MQ=255)

                                                                                                                    ccgcccccACGCCAGAAAGAACCCTACCAGCGGAAGCTGGCACGTCTACAAATGCCTACGCCTTCGCCCAGTGCGAACAATGCCAGCGTCGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACt                  <  2:3449409/137‑1 (MQ=12)

                                                                                                                      ccccccACGTCCAGAAGAACTCAAACACCTGAAGCTAAATCTGCAACCGATTCCGTCACCCAAGGCCAGTGCGAGCAATATCAAAGCGGTTTTTGGTGCGAGGGTGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa                <  2:2527322/139‑1 (MQ=16)

                                                                                                                      aCCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa                <  2:119835/140‑1 (MQ=255)

                                                                                                                      aCCCCCACATACGTAAAAACACGAACACCTGAAGCTAGATCGTCTACAGAGTCCGACACCCACGCCCAGTACGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa                <  2:2658531/140‑1 (MQ=21)

                                                                                                                        ccccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCTATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCAACGTCCGTAAGGACACTTaca              >  2:287396/1‑140 (MQ=255)

                                                                                                                          ccACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGTCCTTTAACCCCCACGTCCGTAAGGACACTAACAcc            >  2:3051014/1‑140 (MQ=255)

                                                                                                                          ccACGTCCCTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCGCTTTCTCCCAGTGCGAACAATATAAACGAGGTTTTTGGTGCGAGGGGCGTGCCTTTAACCCCCACGTCCTTAAGGACACTAACAcc            >  2:3192594/1‑140 (MQ=25)

                                                                                                                            aCGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTg          >  1:2060721/1‑140 (MQ=255)

                                                                                                                            aCGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTg          >  1:1283031/1‑140 (MQ=255)

                                                                                                                                  gTAAGAACACTAACCCCTGAAGCTAGCGCGTCTACCAATTCCGCCACCCTCGCCCAGTTCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTa    <  1:4465171/140‑1 (MQ=255)

                                                                                                                                  gTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGTTTTTTGGTGCGAGGGGGATGCCTTTAACCCCCACTTCCGGAGCGACACTAACACCTGAACCTa    >  2:3025330/1‑140 (MQ=25)

                                                                                                                                  gTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATAACAACGTGGTTTTTGGTGCGAGGGGGGGGCCTTGAACCCCCACGTCCGCAAGGACACTAACACCTGAAGCTa    >  2:1153683/1‑140 (MQ=255)

                                                                                                                                   tAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCTCACGTCCGTAAGGACACTAACACCTGAAGCTAg   >  2:1564512/1‑140 (MQ=255)

                                                                                                                                   tAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg   >  1:1211044/1‑140 (MQ=255)

                                                                                                                                   tAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg   >  1:2472905/1‑140 (MQ=255)

CCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTACTTTTTTTGATATCGCCTCGTTTGGTGCG‑AGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGC  >  NZ_CP025268/4608020‑4608290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`