| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,189,874 | 0 | C | A | 38.1% | 65.5 / 47.2 | 42 | R346L (CGT→CTT) | yehB | putative fimbrial usher protein YehB |
| Reads supporting (aligned to +/- strand): ref base C (10/16); new base A (16/0); total (26/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.09e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATCGAGCCACCATACAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑ACGACCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑ATTTGGCACCGCTGCATAAGGCACCAGATAGGTGGTTACCGAGCCGTCCGC > NC_000913/2189778‑2189968 | accGAGCCACCATACAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑Ac < 1:18789208/95‑1 (MQ=255)aTCGAGCCACCATACAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑Ac < 1:10410706/97‑1 (MQ=255)aTCGAGCCACCATACAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑Ac < 1:3608473/97‑1 (MQ=255) tCGAGCCCCCATACAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑ACg < 1:14901303/97‑1 (MQ=255) tCGAGCCACCATACAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑ACg < 1:6541882/97‑1 (MQ=255) tCGAGCCACCATACAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑ACg < 1:2208886/97‑1 (MQ=255) cggAGCCACCATACAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑ACg < 1:6152216/95‑1 (MQ=255) gacccaccaTACAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑ACGAc < 1:11584636/94‑1 (MQ=255) gAGCCACCATACAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑ACGAc < 1:13100350/97‑1 (MQ=255) gAGCCACCATACAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑ACGAc < 1:10456833/97‑1 (MQ=255) gAGCCACCATACAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑ACGAc < 1:17636435/97‑1 (MQ=255) aGCCACCATACAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑ACGAcc < 1:4551967/97‑1 (MQ=255) aGCCACCATACAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑ACGAcc < 1:4297780/97‑1 (MQ=255) aGCCACCATACAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑ACGAcc < 1:10663778/97‑1 (MQ=255) gCCACCATACAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑ACGAccc < 1:19684859/97‑1 (MQ=255) tcaccaTACAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑ACGACccg < 1:10428311/96‑1 (MQ=255) ccaccaTACAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑ACGACccg < 1:4338323/97‑1 (MQ=255) ccaccaTACAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑ACGACccg < 1:834849/97‑1 (MQ=255) caccaTACAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑AAAAcccac > 1:1134227/1‑95 (MQ=255) accaTACAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑ACGACccgcc < 1:2930702/97‑1 (MQ=255) tACAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑AAAAAccgccccca > 1:353976/1‑93 (MQ=255) tACAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑AAAAAACGCCGCtc > 1:18281140/1‑96 (MQ=255) gCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑AAAAAACGCCGCCAAATc > 1:17594000/1‑97 (MQ=255) aaTAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑AAAAAACGCCGCTACAACataat > 1:12065623/1‑95 (MQ=255) tAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑ACGACCAGGCACGCTATCATATTTc > 1:14948848/1‑97 (MQ=255) attattAAAACCATACTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑AAAACACCCCGCCAAATCATATTTTGac > 1:1887782/1‑97 (MQ=255) attaAAACCATACTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑ACGACCCCCCGCCAAATCATATTTTGACACg > 1:3478736/1‑97 (MQ=255) taAAACCATACTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑AAAACCCCCCGCGGAATCATATTTTTacaccac > 1:4731575/1‑94 (MQ=255) aCCATACTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑AAGAACCGCCGCTAAATCATATTTCGACACCCCcggc > 1:19341998/1‑96 (MQ=255) aTACTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑AAAAACCACCGCCACAACATAATTTTACACGCCCGGtgtc > 1:20166202/1‑94 (MQ=255) cTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑AAAACCCCCCCCCAAATCATATTTTCACACCCCGGGGTggggc > 1:17097655/1‑93 (MQ=255) cTGACACCCCGCCTGGAAAAAATCTT‑TTTGTTTG‑CCCCCCCCTTCAATATGGCT‑CCGCCCCGCCGCTAAATCATATTTCGACACGCCGGGTTgcagc < 1:10035557/97‑1 (MQ=255) tGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑AAAACCCCCCCCTAAAACATATTTTTACACGCCGCGGTgcagcg > 1:16129377/1‑96 (MQ=255) aTAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑AAAAACCCCCGCTCAAACATATTTTTACACCCCGCGGTGGAGCatc > 1:17745253/1‑96 (MQ=255) cacccccccTGAAAAAAATCTT‑TTTTTTTC‑CCCCCCCCTTAAATATGCCT‑CCCCCCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCat‑at < 1:10274706/94‑1 (MQ=255) acccccccTGGAAAAAATCCCTTTTTTTTG‑CCCCCCCCTTCAATATGGCC‑CCGCCCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCat‑at < 1:10752520/95‑1 (MQ=255) accccGCCGGAAAAAAATCCT‑TTTGTTTC‑CCCCCCCCTTCAATAGGGCT‑ACGCCCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑Att < 1:12502290/95‑1 (MQ=255) ccccGCCTGGACAAAATCTT‑TTTGTTTC‑CTCCCCCCTTCAATAGGGCT‑ACGCCCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑Attt < 1:3178985/96‑1 (MQ=255) ccccGCCTGCAAAAAATCTT‑TTTGTGTG‑CTCCCCCCTTAAATAGGGCC‑CCCCCCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑Attt < 1:162716/96‑1 (MQ=255) ccccGCCTCGAGAAAATCAT‑TTTTTTTT‑CCCGCCCTTTTATTACGGCA‑ACGCCCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑Attt < 1:20515438/96‑1 (MQ=255) cccGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑AAAACCCCCCCCCCAAACAAAATTTGTCACGCCCGGGCGCAGCAC‑Atttt > 1:18634573/1‑96 (MQ=255) cGCCTGAAAAAAATCTT‑TTTGTTGC‑CCCCCCCCTACAATAGGGCT‑CCGCCCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑ATTTGGc < 1:14156085/97‑1 (MQ=255) gCCTGAAAAAAATC‑C‑CTTGTTTGCCCCCCCCCTTAAATATGCCA‑CCCCCCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑ATTTGGCa < 1:9929613/97‑1 (MQ=255) tGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑AAGACACCCCGCTCAAACATATTTCTACACGCCCGGGGGCAGCAC‑ATTTTTCAcca > 1:8406281/1‑96 (MQ=255) tGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑AAAAACCCCCGCCAAATAATATTTTTTCACCCCCGGGTGCCGCAT‑ATTTTGCACCg > 1:601857/1‑97 (MQ=255) ggACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCTAAAAACCCCCCCCAAAATCA‑ATTTTTACACGCCCGGGTGCAGCAT‑AATTTGCAcccc > 1:20361017/1‑95 (MQ=255) ggACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑AAAAACCCCCGCCAAAAAAAATTTTCACACGCCCGGGTGCAGCAT‑ATTTTGCACCGc > 1:20961285/1‑97 (MQ=255) ggACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑AAAAACCCCCCCCCAAACATATTTTTTAACGCCCGGGGGCAGCCT‑ATTTTGGACCGc > 1:13344777/1‑97 (MQ=255) caaaaaaTCAT‑TTTGTTTC‑CCCCCCCCTACAAACTGGCA‑ACCCCCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑ATTTGGCACCGCt < 1:9552194/94‑1 (MQ=255) aaaaaaTCGT‑TTTGTTGC‑CCCCCCCCTTAAATAGGGCT‑CCCCCCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑ATTTGGCACCGCTg < 1:13527125/95‑1 (MQ=255) cAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑AAAAACCCCCCCCCAATCATAATTTCACACGCCGGGGTGGCGCCA‑ATTTTGGTCCGCTGc > 1:18154331/1‑97 (MQ=255) cAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑AAAAAACCCCGCCCAAACATATTTTTTCACCCCGGGGTGCGGCAT‑AATTTGCACCGCTGc > 1:7649895/1‑97 (MQ=255) aaTCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑AAAAACCGCCGCTAAAAACTATTTTGACACGCCCGGGGGCAGCAC‑ATTTTGCAACTCTGCATa > 1:13835344/1‑97 (MQ=255) tctttttgtttc‑CCCCCCCCTTAAATAGGGCT‑CCGCCCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑ATTTGGCACCGCTGCATAAg < 1:8399143/93‑1 (MQ=255) cAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑AAAACCCCCCGCCAAAAAATATTTTTACAACCCCGGGTGCGGCATAATTTGGC‑CCGCTGCACAAgg > 1:15052251/1‑97 (MQ=255) tgtgttcg‑CCCGCCCCTTCAATATGCCC‑CCGCCCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑ATTTGGCACCGCTGCATAAGGCAc < 1:1677299/95‑1 (MQ=255) tgtttc‑CCCCCCCCTTCAATATGGCT‑CCCCCCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑ATTTGGCACCGCTGCATAAGGCACCa < 1:8796241/97‑1 (MQ=255) gttttg‑CCCCCCCCTTAAATAGGCCT‑CCCCCCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑ATTTGGCACCGCTGCATAAGGCACCa < 1:1809445/95‑1 (MQ=255) cccccctTTAAATAGGGCT‑CCCCCCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑ATTTGGCACCGCTGCATAAGGCACCAGATAggt < 1:271068/94‑1 (MQ=255) ccccTTCAATAGGGCC‑ACCCCCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑ATTTGGCACCGCTGCATAAGGCACCAGATAggtggt < 1:7972154/97‑1 (MQ=255) cccTTCATTAGGGCC‑CCCCCCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑ATTTGGCACCGCTGCATAAGGCACCAGATAGGTGGtt < 1:15072047/97‑1 (MQ=255) tttCAATAGGGCT‑CCCCCCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑ATTTGGCACCGCTGCATAAGGCACCAGATAGGTGGTTAc < 1:18203941/96‑1 (MQ=255) gttCAATGCGGCT‑CCCCCCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑ATTTGGCACCGCTGCATAAGGCACCAGATAGGTGGTTAc < 1:506004/96‑1 (MQ=255) cTTGAATATGCCT‑CCGCCCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑ATTTGGCACCGCTGCATAAGGCACCAGATAGGTGGTTAc < 1:6843661/97‑1 (MQ=255) tCAATATCGCT‑CCCCCCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑ATTTGGCACCGCTGCATAAGGCACCAGATAGGTGGTTACCg < 1:6604941/97‑1 (MQ=255) caaaTAGGGCT‑CCCCCCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑ATTTGGCACCGCTGCATAAGGCACCAGATAGGTGGTTACCg < 1:4789930/95‑1 (MQ=255) aaaTGTGGCT‑CCCCCCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑ATTTGGCACCGCTGCATAAGGCACCAGATAGGTGGTTACCGa < 1:7033373/96‑1 (MQ=255) tGGCT‑ACGACCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑ATTTGGCACCGCTGCATAAGGCACCAGATAGGTGGTTACCGAGCCGt > 1:11678065/1‑97 (MQ=255) tGGCT‑ACGACCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑ATTTGGCACCGCTGCATAAGGCACCAGATAGGTGGGTACCCAGCCGt > 1:12017303/1‑97 (MQ=255) ggCT‑ACGACCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑ATTTGGCACCGCTGCATAAGGCACCAGATAGGTGGTTACCGAGCCGTc > 1:20477716/1‑97 (MQ=255) gCT‑ACGACCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑ATTTGGCACCGCTGCATAAGGCACCAGATAGGTGGTTACCGCGCCGTcc > 1:1948705/1‑97 (MQ=255) gCT‑ACGACCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑ATTTGGCACCGCTGCATAAGGCACCAGATAGGTGGTTACCGAGCCGTcc > 1:20173163/1‑97 (MQ=255) cT‑ACGACCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑ATTTGGCACCGCTGCATAAGGCACCAGATAGGTGGTTACCGAGCCGTCCg > 1:18914823/1‑97 (MQ=255) t‑ACGACCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑ATTTGGCACCGCTGCATAAGGCACCAGATAGGTGGTTACCGAGCCGTCCGc > 1:1308715/1‑97 (MQ=255) t‑ACGACCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑ATTTGGCACCGCTGCATAAGGCACCAGATAGGTGGTTACCGAGCCGTCCGc > 1:14984948/1‑97 (MQ=255) t‑ACGACCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑ATTTGGCACCGCTGCATAAGGCACCAGATAGGTGGTTACCGAGCCGTCCGc > 1:20180745/1‑97 (MQ=255) t‑ACGACCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑ATTTGGCACCGCTGCATAAGGCACCAGATAGGTGGTTACCGAGCCGTCCGc > 1:19531464/1‑97 (MQ=255) | ATCGAGCCACCATACAGCGTCAATAAATTATTAAAACCATACTGATAACCCGCCTGGACAAAATCAC‑TTTGTTTG‑CTCGCCCCTTCAATATGGCT‑ACGACCCGCCGCTAAATCATATTTCGACACGCCGGGTTGCAGCAT‑ATTTGGCACCGCTGCATAAGGCACCAGATAGGTGGTTACCGAGCCGTCCGC > NC_000913/2189778‑2189968 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |