BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,486,372	G→T	A85S (GCA→TCA)	crp →	DNA‑binding transcriptional dual regulator CRP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,486,372	0	G	T	100.0%	3676.6 / NA	887	A85S (GCA→TCA)	crp	DNA‑binding transcriptional dual regulator CRP
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (472/415); total (472/415)

Only 100 of 887 total aligned reads displayed.

TCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTT  >  NC_000913/3486274‑3486469

     gACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGt                                                                                            >  1:2111444/1‑101 (MQ=255)

     gACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGt                                                                                            <  2:20139469/101‑1 (MQ=255)

       cGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTAc                                                                                          >  1:2796678/1‑101 (MQ=255)

       cGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTAc                                                                                          <  2:11007293/101‑1 (MQ=255)

        gAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACg                                                                                         <  1:8674523/101‑1 (MQ=255)

          agagGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTg                                                                                       <  2:1831315/101‑1 (MQ=255)

          agagGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTg                                                                                       >  1:16633454/1‑101 (MQ=255)

          agagGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTg                                                                                       >  2:16592944/1‑101 (MQ=255)

           gagGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGc                                                                                      >  1:15835124/1‑101 (MQ=255)

           gagGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGc                                                                                      <  2:17126820/101‑1 (MQ=255)

           gagGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGc                                                                                      >  1:14002830/1‑101 (MQ=255)

           gagGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGc                                                                                      >  2:9704075/1‑101 (MQ=255)

            agGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCg                                                                                     <  2:817819/101‑1 (MQ=255)

            agGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCg                                                                                     <  2:4190170/101‑1 (MQ=255)

             gggTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGa                                                                                    >  1:5769715/1‑101 (MQ=255)

             gggTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGa                                                                                    <  1:6746548/101‑1 (MQ=255)

             gggTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGa                                                                                    <  2:15203696/101‑1 (MQ=255)

              ggTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGaa                                                                                   >  2:462912/1‑101 (MQ=255)

               gTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGaaa                                                                                  <  2:16855635/101‑1 (MQ=255)

               gTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGaaa                                                                                  >  2:11770545/1‑101 (MQ=255)

                  aaGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAAcc                                                                               <  2:6868194/101‑1 (MQ=255)

                  aaGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAAcc                                                                               >  1:8377813/1‑101 (MQ=255)

                     aaaTGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGcc                                                                            >  1:14278999/1‑101 (MQ=255)

                     aaaTGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGcc                                                                            <  2:18016208/101‑1 (MQ=255)

                     aaaTGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGcc                                                                            <  1:12239512/101‑1 (MQ=255)

                      aaTGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCt                                                                           >  2:8650627/1‑101 (MQ=255)

                       aTGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCtg                                                                          <  1:13456600/101‑1 (MQ=255)

                       aTGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCtg                                                                          <  2:7761760/101‑1 (MQ=255)

                         gATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCtgtg                                                                        >  2:13674634/1‑101 (MQ=255)

                           tCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGaa                                                                      <  1:18862808/101‑1 (MQ=255)

                            ccTCTCCTATCTGGATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAg                                                                     >  1:11434135/1‑101 (MQ=255)

                            ccTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAg                                                                     >  2:13167913/1‑101 (MQ=255)

                             ctctCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGt                                                                    >  1:17154442/1‑101 (MQ=255)

                             ctctCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGt                                                                    >  1:15466487/1‑101 (MQ=255)

                             ctctCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGt                                                                    <  2:9453968/101‑1 (MQ=255)

                              tctcCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTg                                                                   >  2:16050746/1‑101 (MQ=255)

                              tctcCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTg                                                                   >  1:515065/1‑101 (MQ=255)

                                tcCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGc                                                                 >  2:5323092/1‑101 (MQ=255)

                                 ccTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATTGGTACGTGCGAAAACCGCCTGTGAAGTGGCt                                                                <  1:6962490/101‑1 (MQ=255)

                                 ccTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCAGTGAAGTGGCt                                                                >  2:708297/1‑101 (MQ=255)

                                  cTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGGAAACCGCCTGTGAAGTGGCTg                                                               <  2:10574033/101‑1 (MQ=255)

                                  cTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTg                                                               <  2:19547497/101‑1 (MQ=255)

                                     tCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGaaa                                                            >  1:19822686/1‑101 (MQ=255)

                                     tCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGaaa                                                            <  1:13086133/101‑1 (MQ=255)

                                      cTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAAt                                                           <  2:20400451/101‑1 (MQ=255)

                                      cTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAAt                                                           >  2:11919258/1‑101 (MQ=255)

                                      cTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAAt                                                           >  2:15762145/1‑101 (MQ=255)

                                      cTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAAt                                                           <  1:19044249/101‑1 (MQ=255)

                                       tGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAAtt                                                          <  1:10223823/101‑1 (MQ=255)

                                          aTCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCg                                                       >  1:17992756/1‑101 (MQ=255)

                                            cAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTa                                                     >  2:9870424/1‑101 (MQ=255)

                                            cAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTa                                                     >  1:15046787/1‑101 (MQ=255)

                                             aGGTTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTAc                                                    <  2:3183051/101‑1 (MQ=255)

                                             aGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTAc                                                    <  2:5063568/101‑1 (MQ=255)

                                             aGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTAc                                                    >  2:6404512/1‑101 (MQ=255)

                                             aGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTAc                                                    >  1:4983546/1‑101 (MQ=255)

                                              gggTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACa                                                   >  1:4661587/1‑101 (MQ=255)

                                                gTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACaaa                                                 >  2:2375742/1‑101 (MQ=255)

                                                 tGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACaaaa                                                >  2:19207080/1‑101 (MQ=255)

                                                       tATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTc                                          >  1:7263864/1‑101 (MQ=255)

                                                         ttGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGc                                        <  2:2748170/101‑1 (MQ=255)

                                                          tGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGcc                                       <  1:3933744/101‑1 (MQ=255)

                                                           ggCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCa                                      >  2:9032101/1‑101 (MQ=255)

                                                            gCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCaa                                     <  1:10010712/101‑1 (MQ=255)

                                                              gAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAAtt                                   <  2:13998493/101‑1 (MQ=255)

                                                              gAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAAtt                                   >  1:3135925/1‑101 (MQ=255)

                                                              gAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAAtt                                   >  1:20546992/1‑101 (MQ=255)

                                                               aaCTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTg                                  >  2:3792169/1‑101 (MQ=255)

                                                                 cTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGAt                                >  2:14199414/1‑101 (MQ=255)

                                                                 cTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGAt                                <  2:17719081/101‑1 (MQ=255)

                                                                 cTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGAt                                >  1:14524679/1‑101 (MQ=255)

                                                                  tGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGAtt                               >  2:14903540/1‑101 (MQ=255)

                                                                    ggCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCa                             <  1:12807697/101‑1 (MQ=255)

                                                                    ggCCTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCa                             <  1:8039987/101‑1 (MQ=255)

                                                                       cTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGt                          <  1:6401872/101‑1 (MQ=255)

                                                                       cTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGt                          <  2:1550489/101‑1 (MQ=255)

                                                                       cTGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGt                          >  2:14436952/1‑101 (MQ=255)

                                                                        tGTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTa                         >  1:3582950/1‑101 (MQ=255)

                                                                         gTTTGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTaa                        >  1:7630646/1‑101 (MQ=255)

                                                                          tttGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTaaa                       >  1:17748136/1‑101 (MQ=255)

                                                                            tGAAGAGGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAAcc                     <  2:7530212/101‑1 (MQ=255)

                                                                               agagGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCgg                  <  1:10550943/101‑1 (MQ=255)

                                                                               agagGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCgg                  >  2:5895309/1‑101 (MQ=255)

                                                                                gagGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGa                 >  1:2009521/1‑101 (MQ=255)

                                                                                 agGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGAc                <  1:9729461/101‑1 (MQ=255)

                                                                                 agGGCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGAc                <  1:18633749/101‑1 (MQ=255)

                                                                                   ggCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAt              <  1:8966232/101‑1 (MQ=255)

                                                                                   ggCCAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACAt              >  1:11029249/1‑101 (MQ=255)

                                                                                     ccAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTc            >  1:19482585/1‑101 (MQ=255)

                                                                                     ccAGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTc            >  1:5457808/1‑101 (MQ=255)

                                                                                       aGGAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTg          <  1:11849483/101‑1 (MQ=255)

                                                                                        ggAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGa         >  1:2422600/1‑101 (MQ=255)

                                                                                        ggAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGa         <  1:14746881/101‑1 (MQ=255)

                                                                                         gAACGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGAt        >  2:12847483/1‑101 (MQ=255)

                                                                                          aaCGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATg       >  1:16116570/1‑101 (MQ=255)

                                                                                            cGTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCg     <  2:11496188/101‑1 (MQ=255)

                                                                                             gTAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGt    >  1:9326389/1‑101 (MQ=255)

                                                                                              tAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGtt   >  2:18580981/1‑101 (MQ=255)

                                                                                              tAGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGtt   >  2:12287267/1‑101 (MQ=255)

                                                                                               aGCTCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGttt  <  2:10229148/101‑1 (MQ=255)

TCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGATTCAGGTAAACCCGGACATTCTGATGCGTTT  >  NC_000913/3486274‑3486469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`