Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 1,916,379 Δ1 bp pseudogene (355/598 nt) llmg_pseudo_59 ← pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM4066711,916,3790T.99.3% 5211.9 / 18.8 1104pseudogene (355/598 nt)llmg_pseudo_59pseudogene
Reads supporting (aligned to +/- strand):  ref base T (6/2);  new base . (537/558);  total (543/561)
Fisher's exact test for biased strand distribution p-value = 1.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1122 total aligned reads displayed.

CGCGGCTTCAATATCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATATGGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCATTTTTAGATG  >  AM406671/1916235‑1916522
                                                                                                                                                |                                                                                                                                               
cgcgGCTTCAATATCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑Ggaag                                                                                                                                            >  1:7476782/1‑149 (MQ=255)
  cgGCTTCAATATCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑Ggaagaa                                                                                                                                          <  1:2557669/149‑1 (MQ=255)
   ggCTTCAATATCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGaaa                                                                                                                                         >  2:807805/1‑149 (MQ=255)
    gCTTCAATATCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTCAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAAt                                                                                                                                        >  1:1598622/1‑149 (MQ=255)
    gCTTCAATATCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAAt                                                                                                                                        >  1:4788306/1‑149 (MQ=255)
    gCTTCAATATCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCATCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTACAAAAAATA‑GGAAGAAAt                                                                                                                                        >  2:8300350/1‑149 (MQ=255)
     cTTCAATATCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAAtt                                                                                                                                       <  1:399651/149‑1 (MQ=255)
       tCAATATCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTcc                                                                                                                                     <  1:2991812/149‑1 (MQ=255)
        cAATATCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTACAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCa                                                                                                                                    <  2:5340741/149‑1 (MQ=255)
        cAATATCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCa                                                                                                                                    <  2:6494568/149‑1 (MQ=255)
        cAATATCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCa                                                                                                                                    <  1:6408398/149‑1 (MQ=255)
          atatCCAATTTTCGGTCACCGATATAGTAGGTTTCTTCAGTCTTTAAATTATATTTTTTAACTTAGTAGTTGATTGCCTCAGGATTTGGTTTTCTTTTAAAAATATTTTCAGAAGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAg                                                                                                                                  <  1:5658501/149‑1 (MQ=255)
          atatCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATCTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAg                                                                                                                                  <  1:6283176/149‑1 (MQ=255)
            atCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAgtt                                                                                                                                >  2:4488649/1‑149 (MQ=255)
            atCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAgtt                                                                                                                                >  1:4640786/1‑149 (MQ=255)
             tCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAgttg                                                                                                                               <  1:3621617/149‑1 (MQ=255)
              ccAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAgttgt                                                                                                                              >  1:10440042/1‑149 (MQ=255)
               cAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAgttgtt                                                                                                                             >  2:753013/1‑149 (MQ=255)
               cAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAgttgtt                                                                                                                             >  1:3423559/1‑149 (MQ=255)
               cAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAgttgtt                                                                                                                             <  2:1279748/149‑1 (MQ=255)
                 aTTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTg                                                                                                                           >  1:6863311/1‑149 (MQ=255)
                  ttttCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGa                                                                                                                          >  2:7385148/1‑149 (MQ=255)
                   tttCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAg                                                                                                                         >  1:9477189/1‑149 (MQ=255)
                    ttCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACACCTTCCAAAAAATA‑GGAAGAAATACCACGTTTTTTGAGt                                                                                                                        >  2:6926438/1‑149 (MQ=255)
                     tCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAAT‑TGGAAGAAATTCCAAGTTGTTTGAGTa                                                                                                                       >  1:6701115/1‑149 (MQ=255)
                      cGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTaa                                                                                                                      <  2:3609629/149‑1 (MQ=255)
                      cGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTaa                                                                                                                      >  1:8079899/1‑149 (MQ=255)
                        gTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAAt                                                                                                                    >  1:2218540/1‑149 (MQ=255)
                        gTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAAt                                                                                                                    >  1:4158095/1‑149 (MQ=255)
                        gTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAAt                                                                                                                    <  2:10480038/149‑1 (MQ=255)
                            ccGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCaaa                                                                                                                >  2:4136641/1‑149 (MQ=255)
                               atatAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGGGTAAATCAAAGGc                                                                                                             <  2:2246785/149‑1 (MQ=255)
                               atatAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGc                                                                                                             >  1:1772332/1‑149 (MQ=255)
                                tataGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAAGTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAACAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCa                                                                                                            >  2:6316806/1‑149 (MQ=255)
                                 ataGTAGGTTTCATCAGTCTTTAAATTATTTTTTTTAACTGAGTAATTGATTGCCTGAGGATTTGGTTTTCGTATAAAATTATTTTCAGGTGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCAt                                                                                                           <  2:1750841/149‑1 (MQ=255)
                                 ataGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCAt                                                                                                           <  2:2860442/149‑1 (MQ=255)
                                       ggTTTCATCAGTCTTTAAATTATATTTTTTAATTATGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAAGTACTTCCAAAAAATAT‑GATGAAATTACAAGTTGTTTGAGTAAATAAAATGCAtttttta                                                                                                     >  2:92532/1‑147 (MQ=255)
                                       ggTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGc                                                                                                     <  1:6478277/149‑1 (MQ=255)
                                       ggTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGc                                                                                                     >  1:9716114/1‑149 (MQ=255)
                                         tttCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCt                                                                                                   <  1:7630062/149‑1 (MQ=255)
                                         tttCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCt                                                                                                   <  1:1160555/149‑1 (MQ=255)
                                            catcaGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GTAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTtg                                                                                                <  1:7076913/149‑1 (MQ=255)
                                            catcaGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTtg                                                                                                >  2:2098544/1‑149 (MQ=255)
                                              tcaGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTtgtg                                                                                              <  1:8428554/149‑1 (MQ=255)
                                                aGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCTTTTGTGat                                                                                            >  2:614546/1‑148 (MQ=255)
                                                aGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAg                                                                                            >  2:558652/1‑149 (MQ=255)
                                                 gTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGt                                                                                           >  2:8980880/1‑149 (MQ=255)
                                                 gTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCATTGTGAGt                                                                                           >  1:6039778/1‑149 (MQ=255)
                                                   cTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGtat                                                                                         <  1:3086245/149‑1 (MQ=255)
                                                   cTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGATAACAACTTCCAAAAAATA‑GGAAGAAATTTCAAGTTGCTTGAGTAAATCAAAGTCATTTTTGCCTTTGTGATtat                                                                                         >  2:1905301/1‑149 (MQ=255)
                                                      tAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGTCTTTGTGAGTATaaa                                                                                      >  2:7126397/1‑149 (MQ=255)
                                                      tAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATaaa                                                                                      <  1:9963681/149‑1 (MQ=255)
                                                       aaaTTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAAt                                                                                     <  1:1347581/149‑1 (MQ=255)
                                                        aaTTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATg                                                                                    <  1:736310/149‑1 (MQ=255)
                                                         aTTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGa                                                                                   <  1:4478283/149‑1 (MQ=255)
                                                            atatTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGt                                                                                >  2:3021256/1‑149 (MQ=255)
                                                                tttttAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTg                                                                            >  2:1176912/1‑149 (MQ=255)
                                                                 ttttAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGa                                                                           >  2:8536070/1‑149 (MQ=255)
                                                                 ttttAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGa                                                                           <  2:9637284/149‑1 (MQ=255)
                                                                         aaGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GTAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTc                                                                   >  1:2418447/1‑149 (MQ=255)
                                                                         aaGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTc                                                                   >  1:2025501/1‑149 (MQ=255)
                                                                          aGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCt                                                                  >  2:8740753/1‑149 (MQ=255)
                                                                           gTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCtt                                                                 <  2:7884682/149‑1 (MQ=255)
                                                                            tAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCttt                                                                >  2:2582491/1‑149 (MQ=255)
                                                                              attgattgCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCACGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCttttt                                                              <  1:5373062/149‑1 (MQ=255)
                                                                               ttgattgCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTTCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTg                                                             >  2:467408/1‑149 (MQ=255)
                                                                               ttgattgCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTg                                                             <  1:8281408/149‑1 (MQ=255)
                                                                               ttgattgCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTg                                                             <  1:3291871/149‑1 (MQ=255)
                                                                                      ccTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGtccaa                                                      >  2:6673479/1‑149 (MQ=255)
                                                                                      ccTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGtccaa                                                      >  1:4994343/1‑149 (MQ=255)
                                                                                       cTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGtccaat                                                     <  1:8677792/149‑1 (MQ=255)
                                                                                          aGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGtccaatcca                                                  >  2:2436538/1‑149 (MQ=255)
                                                                                                 ggTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAActtc                                           <  2:9145210/149‑1 (MQ=255)
                                                                                                  gTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAActtct                                          <  2:4293888/149‑1 (MQ=255)
                                                                                                  gTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAActtct                                          <  2:103451/149‑1 (MQ=255)
                                                                                                    tttctttcAAAATTATTTTCGGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATGCCAAGTTGTTTGAGTAAATCAAAGGCATTTGTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCttt                                        <  2:3893191/149‑1 (MQ=255)
                                                                                                       cTTTTAAAATAATTTTCATATGTAAAAACTACAAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGc                                     <  2:1593467/149‑1 (MQ=255)
                                                                                                         tttAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTNTGAGTCCAATCCAAAACTTCTTTAGCAc                                   <  1:5820856/149‑1 (MQ=255)
                                                                                                          ttAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCAct                                  >  2:5790528/1‑148 (MQ=255)
                                                                                                             aaaTTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTAc                               <  2:315868/149‑1 (MQ=255)
                                                                                                              aaTTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGtttt                                                                    <  2:5234339/111‑1 (MQ=255)
                                                                                                              aaTTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTAcc                              <  2:5916495/149‑1 (MQ=255)
                                                                                                              aaTTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTAcc                              >  1:9117131/1‑149 (MQ=255)
                                                                                                               attattTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCa                             <  1:9378574/149‑1 (MQ=255)
                                                                                                                 tattTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCAtt                           >  1:281781/1‑149 (MQ=255)
                                                                                                                     ttCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAatt                       <  2:4819654/149‑1 (MQ=255)
                                                                                                                       cAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAatttt                     <  1:10293430/149‑1 (MQ=255)
                                                                                                                       cAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAatttt                     <  1:3747892/149‑1 (MQ=255)
                                                                                                                         gagGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAattttat                   <  2:505332/149‑1 (MQ=255)
                                                                                                                          agGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCACAACTTCTTTAGCACCTACCATTAattttatt                  <  2:146156/149‑1 (MQ=255)
                                                                                                                          agGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAattttatt                  <  1:6541350/149‑1 (MQ=255)
                                                                                                                           ggTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAattttattt                 <  1:7860874/149‑1 (MQ=255)
                                                                                                                             tAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTg               <  1:8908149/149‑1 (MQ=255)
                                                                                                                               acaacTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTc             <  1:10025300/149‑1 (MQ=255)
                                                                                                                                  aaaaaaaaaaaaTA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCAtt          <  2:9442167/143‑1 (MQ=255)
                                                                                                                                    ttCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCAtttt        <  2:10142276/149‑1 (MQ=255)
                                                                                                                                       cAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCATTTTTAg     <  1:5127626/149‑1 (MQ=255)
                                                                                                                                       cAAAAAATA‑GGAAGAAATTCCAAGTTGTTCGAGTAAAACAAAAGCATCATTGCCTTTGTGAGTATAAATGAAGTTTTGATTGTTTTCTTTTTGAGTCCAATTCAGAACTTCTCTTGCACCTACCATTAATTTTGTTTTGTCACTTTTAg     >  2:3441225/1‑149 (MQ=255)
                                                                                                                                        aaaaaaTA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCATTTTTAGa    >  1:5505721/1‑149 (MQ=255)
                                                                                                                                         aaaaaTA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCATTTTTAGAt   <  2:7689692/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
CGCGGCTTCAATATCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATATGGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCATTTTTAGATG  >  AM406671/1916235‑1916522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑