BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	1,921,694	Δ1 bp	intergenic (‑184/‑201)	llmg_1941 ← / → glnP	conserved hypothetical protein/glutamine ABC transporter permease and substrate binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	1,921,693	0	G	.	98.6%	5658.8 / 60.6	1208	intergenic (‑183/‑202)	llmg_1941/glnP	conserved hypothetical protein/glutamine ABC transporter permease and substrate binding protein
Reads supporting (aligned to +/- strand): ref base G (12/5); new base . (593/595); total (608/600)
Fisher's exact test for biased strand distribution p-value = 1.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1245 total aligned reads displayed.

CTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAACTTTTCTTATATTTTATTCAGGAGAACTCTTCAAATTTCAACAAATTTCAAAATGTCAGGAAAACCTAAACATTCAACTTGATTTCTAGAACAATTTTTGTGATATAATTTTTCCCAGAAATAAAATAAACTGAGCTTGTCTTCCTAAATATAGGGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCA  >  AM406671/1921539‑1921845

cTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCAg                                                                                                                                                          <  2:4413901/149‑1 (MQ=255)

cTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑AAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCAg                                                                                                                                                          >  1:6829046/1‑149 (MQ=255)

 ttACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCAga                                                                                                                                                         >  2:3540402/1‑148 (MQ=255)

  tACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTAAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATGTC‑ACAAA‑TTCAAAATGTCAgaa                                                                                                                                                        <  2:1640093/149‑3 (MQ=255)

     agtagtTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAAc                                                                                                                                                    >  1:1617937/1‑149 (MQ=255)

      gcaggtCTGTTTACGTAAATAGAATACATAAATCTCGCTATCAAGGACGCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑CCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAAcc                                                                                                                                                   >  2:580742/6‑149 (MQ=255)

       tagtTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCt                                                                                                                                                  >  2:1157355/1‑149 (MQ=255)

       tagtTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTATACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCt                                                                                                                                                  <  2:1896714/149‑1 (MQ=255)

         gtTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCTaa                                                                                                                                                <  1:7052905/149‑1 (MQ=255)

          ttCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCTaac                                                                                                                                               >  2:8693522/1‑148 (MQ=255)

           tCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCTaaca                                                                                                                                              >  1:534072/1‑147 (MQ=255)

            cTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCTaacat                                                                                                                                             >  1:10044888/1‑146 (MQ=255)

              gTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCTaacatca                                                                                                                                           >  1:2962246/1‑144 (MQ=255)

              gTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCTaacatca                                                                                                                                           >  2:9323028/1‑144 (MQ=255)

              gTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCTaacatca                                                                                                                                           >  2:3414028/1‑144 (MQ=255)

              gTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCTaacatca                                                                                                                                           >  2:10345583/1‑144 (MQ=255)

              gTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCTaacatca                                                                                                                                           >  2:5328865/1‑144 (MQ=255)

                   cTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTg                                                                                                                                    <  2:7186861/149‑1 (MQ=255)

                   cTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTg                                                                                                                                    <  2:4226693/149‑1 (MQ=255)

                           gAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAGa                                                                                                                           <  1:8017043/149‑1 (MQ=255)

                             aTACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAGaca                                                                                                                         >  1:5013555/1‑147 (MQ=255)

                             aTACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAGaca                                                                                                                         >  2:8523695/1‑147 (MQ=255)

                               aCATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAGacaat                                                                                                                       <  2:5628083/149‑5 (MQ=255)

                                cATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAAtt                                                                                                                     >  1:2190422/1‑149 (MQ=255)

                                cATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAAtt                                                                                                                     <  2:7363512/149‑1 (MQ=255)

                                cATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAAtt                                                                                                                     <  1:9282206/149‑1 (MQ=255)

                                aaTAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAAtt                                                                                                                     <  1:3355352/148‑1 (MQ=255)

                                    aaTATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTtg                                                                                                                 <  1:3217506/149‑1 (MQ=255)

                                     atatCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTtgt                                                                                                                <  1:8609207/149‑1 (MQ=255)

                                      tatCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTtgtg                                                                                                               <  1:7895609/149‑1 (MQ=255)

                                      tatCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTtgtg                                                                                                               >  1:6190673/1‑149 (MQ=255)

                                       atCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGa                                                                                                              >  1:5647885/1‑149 (MQ=255)

                                       atCTCTATCAAGCACTCGTACAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGa                                                                                                              <  1:173347/149‑1 (MQ=255)

                                        tctcTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TC‑GGAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGat                                                                                                             >  2:6252237/1‑149 (MQ=255)

                                        gctctATCAAGGACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATAGTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAGAATGTCA‑GAAAACCT‑AACA‑TCAGCTTGA‑TTCTAG‑ACAATTTTTGTGat                                                                                                             <  2:5954686/148‑1 (MQ=255)

                                         ctctATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGata                                                                                                            <  1:9947238/149‑1 (MQ=255)

                                         ctctATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGata                                                                                                            <  2:4998613/149‑1 (MQ=255)

                                         ctctATCAAGCACTCGTTCAATTTTACAAAAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGata                                                                                                            <  2:4716953/149‑1 (MQ=255)

                                             aTCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAAt                                                                                                        <  1:8403669/149‑1 (MQ=255)

                                               cAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAAttt                                                                                                      >  1:669871/1‑149 (MQ=255)

                                                aaGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAAtttt                                                                                                     <  1:7435060/149‑1 (MQ=255)

                                                 aGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAAttttc                                                                                                    >  1:8924464/1‑148 (MQ=255)

                                                 aGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAAttttc                                                                                                    >  2:9448458/1‑148 (MQ=255)

                                                  gCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAAttttcc                                                                                                   >  1:8200488/1‑147 (MQ=255)

                                                  gCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAAttttcc                                                                                                   >  2:2036048/1‑147 (MQ=255)

                                                   cACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAATTTTccc                                                                                                  <  1:5513938/149‑1 (MQ=255)

                                                    aCTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAAttttccca                                                                                                 <  1:7679742/149‑5 (MQ=255)

                                                    aCTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAAttttccca                                                                                                 <  1:9487097/149‑5 (MQ=255)

                                                       cGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAGaa                                                                                             <  1:3714659/149‑1 (MQ=255)

                                                               taaaaaacaaAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑TCAATTTTTGTGATATAA‑TTTTCCCAG‑aataaaataa                                                                                    <  2:7021240/144‑1 (MQ=255)

                                                                  acaacaAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACt                                                                                 <  2:6488701/149‑1 (MQ=255)

                                                                       aaaaaGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCt                                                                            <  1:1518243/149‑1 (MQ=255)

                                                                       aaaaaGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCt                                                                            <  2:7613174/149‑1 (MQ=255)

                                                                        aaaaGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCtt                                                                           <  1:6315295/149‑1 (MQ=255)

                                                                          aaGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGt                                                                         >  2:3175590/1‑149 (MQ=255)

                                                                          aaGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGt                                                                         <  2:3942065/149‑1 (MQ=255)

                                                                            ggACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTCt                                                                       <  1:2331257/149‑1 (MQ=255)

                                                                              aCTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTCtcc                                                                     <  1:5982830/149‑3 (MQ=255)

                                                                              aCTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTCtcc                                                                     >  2:2772807/1‑147 (MQ=255)

                                                                              aCTTTTTGACAATATAAAC‑TTTCTTATANTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTCtcc                                                                     >  1:5794055/1‑147 (MQ=255)

                                                                                 ttttGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTaa                                                                 <  2:3755172/149‑1 (MQ=255)

                                                                                   ttGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAat                                                               >  1:723875/1‑149 (MQ=255)

                                                                                   ttGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAat                                                               <  2:7470431/149‑1 (MQ=255)

                                                                                    tGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAata                                                              <  1:9719215/149‑1 (MQ=255)

                                                                                     gACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAatat                                                             <  2:1321143/149‑1 (MQ=255)

                                                                                        aaTATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAgg                                                          <  2:8386396/149‑1 (MQ=255)

                                                                                        aaTATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAgg                                                          >  1:2556990/1‑149 (MQ=255)

                                                                                         atatAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAgga                                                         >  1:3556476/1‑148 (MQ=255)

                                                                                         atatAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAgga                                                         <  2:8905077/149‑2 (MQ=255)

                                                                                          tataAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAggaa                                                        >  2:10130216/1‑147 (MQ=255)

                                                                                          tataAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAggaa                                                        <  2:8040099/149‑3 (MQ=255)

                                                                                            taAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAggaaac                                                      >  1:4364993/1‑145 (MQ=255)

                                                                                               aactttCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTa                                                   <  1:4631356/146‑1 (MQ=255)

                                                                                                 ctttCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTaaa                                                 >  1:6097954/2‑149 (MQ=255)

                                                                                                   ttCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAg                                               <  1:10436713/149‑1 (MQ=255)

                                                                                                     cTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGaa                                             >  1:4192023/1‑149 (MQ=255)

                                                                                                      ttATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAAt                                            <  1:10205593/149‑1 (MQ=255)

                                                                                                      ttATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAAt                                            >  1:2711110/1‑149 (MQ=255)

                                                                                                       tataTTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATg                                           <  2:6848412/149‑1 (MQ=255)

                                                                                                       tataTTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATg                                           <  1:1166013/149‑1 (MQ=255)

                                                                                                        atatTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGt                                          >  1:3997176/1‑149 (MQ=255)

                                                                                                        atatTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGt                                          >  2:959103/1‑149 (MQ=255)

                                                                                                         tatTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGtt                                         <  2:9112436/149‑1 (MQ=255)

                                                                                                               ttatcagagaACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTc                                     <  2:7742195/143‑1 (MQ=255)

                                                                                                                tatcagagaACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTcc                                    >  2:817607/6‑149 (MQ=255)

                                                                                                                            tctCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTc                         <  1:196192/147‑1 (MQ=255)

                                                                                                                              tCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTTATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGCATGTTATCCCCATTTTTATTCag                       >  2:8995280/1‑149 (MQ=255)

                                                                                                                              tCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCag                       >  1:8746158/1‑149 (MQ=255)

                                                                                                                                aaatttc‑acaaa‑ttcaaaaTGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCagaa                     <  2:2611104/149‑1 (MQ=255)

                                                                                                                                    tttcacaaa‑ttcaaaATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAgaagaa                  >  2:4590531/5‑149 (MQ=255)

                                                                                                                                     ttcacaaa‑ttcaaaATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑ACTCAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAAc                 >  1:4882103/4‑149 (MQ=255)

                                                                                                                                     ttcacaaa‑ttcaaaATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAAc                 >  1:1314040/4‑149 (MQ=255)

                                                                                                                                      tcacaaa‑ttcaaaATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACa                >  2:6151460/3‑149 (MQ=255)

                                                                                                                                       cacaaa‑ttcaaaATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACAt               <  2:2283692/148‑1 (MQ=255)

                                                                                                                                        acaaa‑ttcaaaATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACAtt              <  1:6450523/149‑1 (MQ=255)

                                                                                                                                                caaAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTaa       <  2:6624831/149‑1 (MQ=255)

                                                                                                                                                   aaTGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAAttt    >  2:9767106/1‑149 (MQ=255)

                                                                                                                                                   aaTGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAAttt    <  1:5244434/149‑1 (MQ=255)

                                                                                                                                                    aTGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTc   >  2:7932958/1‑149 (MQ=255)

                                                                                                                                                    aTGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATA‑GGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTc   <  2:2978046/149‑1 (MQ=255)

CTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAACTTTTCTTATATTTTATTCAGGAGAACTCTTCAAATTTCAACAAATTTCAAAATGTCAGGAAAACCTAAACATTCAACTTGATTTCTAGAACAATTTTTGTGATATAATTTTTCCCAGAAATAAAATAAACTGAGCTTGTCTTCCTAAATATAGGGAAACTAAAAGAATGTTATTCCGATTTTTATTCAGAAGAACATTTTTTTAATTTCA  >  AM406671/1921539‑1921845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`