Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 945,239 +C pseudogene (229/761 nt) tnp904 → pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM406671945,2391.C99.6% 1100.9 / ‑1.5 481pseudogene (229/761 nt)tnp904pseudogene
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base C (222/256);  total (223/258)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 530 total aligned reads displayed.

GGGACGCTATGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑G‑TTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATGGCTGGG  >  AM406671/945091‑945308
                                                                                                                                                      |                                                                     
gggAGGCTATGGTGCGGTTAGAATTCCCCAGGCACTTCATAGTACTGGTAGTGTGACCAACACGAACCGTGTTGGGGAGATGCTGGACTTGATGCGAGGTTATGCCAGGGGAAGCCGTGATGAATGTAAACATTATAACAGAAAAGGA‑g                                                                        >  2:5637902/1‑149 (MQ=255)
 ggACGCTATGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑gc                                                                       <  2:8449906/149‑2 (MQ=38)
 ggACGCTATGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑gc                                                                       >  1:8099036/1‑148 (MQ=38)
 ggACGCTATGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑gc                                                                       <  1:6461578/149‑2 (MQ=38)
 ggACGCTATGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑gc                                                                       <  1:7014686/149‑2 (MQ=38)
 ggACGCTATGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑gc                                                                       >  1:3945411/1‑148 (MQ=38)
  gACGCTATGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑gct                                                                      <  1:10097392/149‑3 (MQ=38)
  gACGCTATGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑gct                                                                      <  1:4197493/149‑3 (MQ=38)
  gACGCTATGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑gct                                                                      <  1:3386822/149‑3 (MQ=38)
  gACGCTATGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑gct                                                                      <  1:947214/149‑3 (MQ=38)
    cGCTATGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑gcttc                                                                    >  1:3039386/1‑145 (MQ=25)
      cTATGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGc                                                                  <  1:7287092/149‑1 (MQ=21)
       tATGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCt                                                                 <  2:1144183/149‑1 (MQ=18)
       tATGGTGCGGTTAGAATTACCAAGGTACTGCATAATCCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGCCTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCt                                                                 <  2:3896060/149‑1 (MQ=11)
        aTGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCtt                                                                <  1:3842863/149‑1 (MQ=17)
        aTGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCtt                                                                >  1:4285209/1‑149 (MQ=17)
        aTGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCtt                                                                >  1:1583438/1‑149 (MQ=18)
         tGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCttt                                                               <  1:4503277/149‑1 (MQ=18)
         tGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCttt                                                               <  2:7352792/149‑1 (MQ=21)
          ggTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTc                                                              <  2:3073710/149‑1 (MQ=21)
           gTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCa                                                             <  1:7186307/149‑1 (MQ=18)
              cGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGa                                                          <  1:2340077/149‑1 (MQ=18)
              cGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGa                                                          >  1:9032683/1‑149 (MQ=21)
               ggTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAGGGA‑GCTTCGCTTTCAAGAc                                                         >  1:5080031/1‑149 (MQ=255)
                gTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACGGAAAAGGA‑GCTTCGCTTTCAAGAcc                                                        <  2:5757045/149‑1 (MQ=255)
                gTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCGAGAcc                                                        >  1:9561368/1‑149 (MQ=255)
                 ttAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGAccc                                                       >  2:8641054/1‑149 (MQ=18)
                 ttAGAATTACCAAGGTACTTCATAATACTGGTATTATAAACAACCTGAAACGTGTGGGGAAACTGATGTACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAGCATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGAccc                                                       >  2:6802851/1‑149 (MQ=11)
                  tAGAATTACCAAGGTACTTCATCATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCa                                                      >  2:1381923/1‑149 (MQ=12)
                  tAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCa                                                      <  1:7392134/149‑1 (MQ=21)
                   aGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCaa                                                     >  2:6943528/1‑149 (MQ=21)
                      aTTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAAttt                                                  >  2:2360233/1‑149 (MQ=21)
                       ttACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATtta                                                 <  1:2815549/149‑1 (MQ=21)
                       ttACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATtta                                                 <  1:9147699/149‑1 (MQ=21)
                       ttACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATtta                                                 <  2:4095850/149‑1 (MQ=17)
                        tACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATttaa                                                <  2:5954725/149‑1 (MQ=21)
                        tACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATttaa                                                <  1:987029/149‑1 (MQ=21)
                        tACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATttaa                                                <  2:500454/149‑1 (MQ=21)
                        tACCAAGGCACTTCATAATACTGGCATTATAACCAACACGAAACGTGCGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGCTATAACTATAAACATTATATCAGCAAAGGA‑GCTTCGCTTTCAAGACCCAATttaa                                                >  2:9251787/1‑149 (MQ=11)
                         aCCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATttaat                                               >  2:3749830/1‑149 (MQ=21)
                          ccAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATttaatt                                              >  2:9384260/1‑149 (MQ=18)
                            aaGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATttaattaa                                            <  2:6451273/149‑1 (MQ=21)
                              ggTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATc                                          <  2:1924911/149‑1 (MQ=21)
                              ggTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATc                                          <  2:4555713/149‑1 (MQ=18)
                              ggTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATc                                          <  1:4847513/149‑1 (MQ=21)
                              ggTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATc                                          >  1:5639844/1‑149 (MQ=21)
                               gTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCa                                         <  1:7821942/149‑1 (MQ=21)
                                tACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAg                                        >  1:109276/1‑149 (MQ=21)
                                   ttCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATc                                     >  2:9556517/1‑149 (MQ=18)
                                   ttCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATc                                     >  1:3458022/1‑149 (MQ=18)
                                     cATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAAGTGAGTCTGTTGACGGGTATATCTCGCAGGTGGAGGTGTCATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAAACCCAATTTAATTAATCAGATCtt                                   >  2:5525670/1‑149 (MQ=2)
                                      ataataCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCttt                                  <  1:8769567/149‑1 (MQ=21)
                                      ataataCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCttt                                  <  1:9712615/149‑1 (MQ=21)
                                      ataataCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCttt                                  <  2:905658/149‑1 (MQ=17)
                                       taataCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTa                                 <  1:8509607/149‑1 (MQ=17)
                                       taataCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTa                                 <  1:6805681/149‑1 (MQ=17)
                                        aataCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTaa                                >  1:5252522/1‑149 (MQ=21)
                                        aataCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTAGATGGGACTTCATGCCAAGGGAAGCCGTTACAAATATAAACATTATAACAGAAAAGGACC‑TTCGCTTTAAAGACACAATTTGATTAATCAGAACTTTaa                                >  2:10243727/1‑149 (MQ=255)
                                         ataCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTaaa                               >  2:216806/1‑149 (MQ=21)
                                         ataCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTaaa                               <  1:4641928/149‑1 (MQ=17)
                                          taCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAg                              >  2:6383715/1‑149 (MQ=21)
                                           aCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGc                             >  2:4004090/1‑149 (MQ=21)
                                           aCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGc                             >  1:2698426/1‑149 (MQ=21)
                                            cTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCa                            >  1:7717437/1‑149 (MQ=21)
                                             tGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCaa                           <  1:6214442/149‑1 (MQ=21)
                                               gTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACa                         <  1:587823/149‑1 (MQ=18)
                                               gTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGAATTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACa                         >  2:797625/1‑149 (MQ=255)
                                                tattatAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACTCAATTTAATTAATCAGATCGTTATAGCAACAg                        <  2:2870912/149‑1 (MQ=11)
                                                tattatAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAg                        <  2:3575326/149‑1 (MQ=21)
                                                tattatAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAg                        >  1:1222959/1‑149 (MQ=21)
                                                 attatAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGc                       <  2:7932706/149‑1 (MQ=18)
                                                 attatAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGc                       >  2:10461401/1‑149 (MQ=17)
                                                 attatAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGc                       >  2:9173131/1‑149 (MQ=21)
                                                   tataACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTc                     <  2:9782969/149‑1 (MQ=17)
                                                   tataACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTc                     <  1:1803760/149‑1 (MQ=21)
                                                     taACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCt                   >  2:2612561/1‑149 (MQ=17)
                                                     taACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCt                   >  1:8357199/1‑149 (MQ=14)
                                                       aCCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACGGCTCCtaa                 >  1:6039030/1‑149 (MQ=9)
                                                       aCCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCtaa                 <  2:4354298/149‑1 (MQ=11)
                                                        ccAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCtaat                <  1:6648181/149‑1 (MQ=14)
                                                        ccAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTATATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCtaat                >  2:7165598/1‑149 (MQ=0)
                                                           acacGAAACGTGTGGGGAAACTGATGCACTTGATGGGTCTTTATGCCAAGGGAAGCCGTTATAAAGATATACATTATAACAGAAAAGGA‑GCTTCGCTTTTAAGACCCAATTTACTTAATCAGATCTTTAAAGCAACAGCTCCTAATaaa             >  2:6618930/1‑149 (MQ=1)
                                                           acacGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATaaa             >  1:7899992/1‑149 (MQ=14)
                                                            cacGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAg            >  1:996191/1‑149 (MQ=14)
                                                            cacGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAg            <  2:6038835/149‑1 (MQ=14)
                                                             acGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGt           <  2:327081/149‑1 (MQ=14)
                                                             acGAAACGTGCGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACAGTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGTTCTTTAAAGCAACAGCTCCTAATAAAGt           >  1:7572701/1‑149 (MQ=1)
                                                              cGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTa          <  1:3192360/149‑1 (MQ=14)
                                                              cGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTa          <  1:8947093/149‑1 (MQ=14)
                                                              cGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTa          <  1:4060545/149‑1 (MQ=14)
                                                              cGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGGTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTa          >  1:3602019/1‑149 (MQ=9)
                                                                aaaCGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACACAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATg        >  2:2734185/1‑149 (MQ=0)
                                                                  aCGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACATCTCCTAATAATGTATGGc      <  2:5589709/149‑1 (MQ=0)
                                                                  aCGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATGGc      >  2:5285675/1‑149 (MQ=14)
                                                                    gtgtGGGGAAACTGATGCACTTGATGGGACTTTATGCTAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATGGCTg    <  2:7462339/149‑1 (MQ=2)
                                                                    gtgtGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATGGCTg    <  1:2202389/149‑1 (MQ=14)
                                                                     tgtgGGGAAAGAGATGCACTTGACGGGACGTTATGGGAAGGGGAGGCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATGGCTgg   >  2:10098576/1‑149 (MQ=11)
                                                                     tgtgGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATGGCTgg   <  2:1711734/149‑1 (MQ=11)
                                                                     tgtgGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATGGCTgg   >  1:10265782/1‑149 (MQ=14)
                                                                      gtgGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATGGCTggg  >  1:5341409/1‑149 (MQ=11)
                                                                                                                                                      |                                                                     
GGGACGCTATGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑G‑TTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATGGCTGGG  >  AM406671/945091‑945308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑