BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	1,210,283	(A)_5→6	pseudogene (515/1046 nt)	llmg_pseudo_39 ←	pseudogene

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	1,210,278	1	.	A	97.4%	3228.2 / 55.2	844	pseudogene (520/1046 nt)	llmg_pseudo_39	pseudogene
Reads supporting (aligned to +/- strand): ref base . (18/4); new base A (414/406); total (434/410)
Fisher's exact test for biased strand distribution p-value = 4.14e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 902 total aligned reads displayed.

TTCAGTCAAAATTTCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑G‑AAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTGCATCAA  >  AM406671/1210130‑1210421

    gTCAAAATTTCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑G‑aaa‑a                                                                                                                                             >  1:6941351/1‑149 (MQ=255)

     tCAAAATTTCTCGAAAACTATCTGTTCGCTCTTGGGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAACTTCTGATAGCCTTTTTGAATCATGTGAGAGACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑G‑aaa‑aa                                                                                                                                            <  1:1238464/149‑1 (MQ=255)

      cAAAATTTCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑G‑aaa‑aaa                                                                                                                                           >  1:6810225/1‑148 (MQ=255)

             tctcGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATACCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGc                                                                                                                                     >  1:6599926/1‑149 (MQ=255)

                   aaaCTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAg                                                                                                                               >  2:10382320/1‑149 (MQ=255)

                     aCTATCTGTTCTCTCCTGAGTCATCCATGTATTGGCCGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTCAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGAt                                                                                                                             <  2:6626798/149‑1 (MQ=255)

                     aCTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGAt                                                                                                                             <  2:7941248/149‑1 (MQ=255)

                     aCTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGAt                                                                                                                             <  1:7678951/149‑1 (MQ=255)

                      cTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATg                                                                                                                            <  2:4096087/149‑1 (MQ=255)

                        aTCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAg                                                                                                                          <  2:9080744/149‑1 (MQ=255)

                        aTCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAg                                                                                                                          <  1:5549946/149‑1 (MQ=255)

                        aTCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAg                                                                                                                          <  1:3151200/149‑1 (MQ=255)

                         tCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTNGA‑GAAAA‑AATATTTGCTGACAGATGAGt                                                                                                                         <  1:5762863/149‑1 (MQ=255)

                          cTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGtt                                                                                                                        <  2:4495017/149‑1 (MQ=255)

                          cTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGtt                                                                                                                        >  1:8319415/1‑149 (MQ=255)

                          cTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGAAAGATGAGtt                                                                                                                        >  2:7359598/1‑149 (MQ=255)

                           tGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTa                                                                                                                       >  1:1097743/1‑149 (MQ=255)

                           tGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑G‑AAATAATATTTGCTGACAGATGAGTTa                                                                                                                       >  2:6829161/1‑149 (MQ=255)

                            gTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTaa                                                                                                                      <  1:2921602/149‑1 (MQ=255)

                              tctctcCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAAt                                                                                                                    >  2:1539597/1‑149 (MQ=255)

                                tctcCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCa                                                                                                                  <  1:7896617/149‑1 (MQ=255)

                                 ctcCTGAGTCATCCATGTATTGGACGAACCACCAATGAAATCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGATTGGGACTGCGTTGGTCTCTTTTTATA‑GAAAA‑AATATTTGCTGCCAGATGAGTTAAATccc                                                                                                                 <  2:1310128/149‑3 (MQ=255)

                                 ctcCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCAc                                                                                                                 >  2:10475121/1‑149 (MQ=255)

                                   ccTTAGTCATCCATGTATTGGACGTACCACCTATGAAATCTAATTTCTGATTGCCTTTTTAACTTATGTTAGTAATTAGTAGTTGAGTGGGATTGCGTTAGTCTCTTTTTAGATT‑CAT‑TATATTTACTGGCAGATGAGTTATATCattt                                                                                                               >  2:4349041/1‑146 (MQ=255)

                                   ccTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGt                                                                                                               >  2:2336382/1‑149 (MQ=255)

                                    cTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTc                                                                                                              >  2:840232/1‑149 (MQ=255)

                                     tGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTcc                                                                                                             <  1:4194361/149‑1 (MQ=255)

                                     tGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTcc                                                                                                             <  1:1899015/149‑1 (MQ=255)

                                       aGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCaa                                                                                                           <  1:44160/149‑1 (MQ=255)

                                         tCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATCGCCTTTTCGAATCATGTGAGAAACAAGTACTTGAGTGGGAGCCCGTTGGTCTCTTTTTAGA‑GCAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGt                                                                                                         >  2:7577656/1‑149 (MQ=255)

                                         tCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGt                                                                                                         <  1:9141354/149‑1 (MQ=255)

                                              cATGTCTTGGACGAACCACCAATCAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAAt                                                                                                    <  1:5928255/149‑1 (MQ=255)

                                                  tATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAgg                                                                                                <  1:5093303/149‑1 (MQ=255)

                                                   aTTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAggg                                                                                               <  1:1416766/149‑1 (MQ=255)

                                                   aTTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAggg                                                                                               >  1:10287687/1‑149 (MQ=255)

                                                        aCGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAAtt                                                                                          >  2:6977004/1‑149 (MQ=255)

                                                          gAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTc                                                                                        >  1:9607924/1‑149 (MQ=255)

                                                            accaccAATGAAAGCTAATTTCTGATAGCCTTTTTTTTTCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCtt                                                                                      >  2:4879213/1‑149 (MQ=255)

                                                             ccaccaATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCttt                                                                                     >  1:9267460/1‑149 (MQ=255)

                                                             ccaccaATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCtt                                                                                      <  1:6154632/148‑1 (MQ=255)

                                                              caccaATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTg                                                                                    >  1:5290761/1‑149 (MQ=255)

                                                               accaATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTgg                                                                                   <  1:9899586/149‑1 (MQ=255)

                                                                     gAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAAt                                                                             <  1:2658898/149‑1 (MQ=255)

                                                                         gCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCt                                                                         >  2:2880396/1‑149 (MQ=255)

                                                                          cTAATTTCTGATAGCCTTTTTGATTCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTa                                                                        >  2:3316400/1‑149 (MQ=255)

                                                                          cTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTa                                                                        >  2:8592110/1‑149 (MQ=255)

                                                                           tAATTTCTGATAGCCTTTGTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCCGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTaa                                                                       <  2:775927/149‑1 (MQ=255)

                                                                            aaTTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAag                                                                      <  1:234251/149‑1 (MQ=255)

                                                                             aTTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAaga                                                                     >  1:8511094/1‑149 (MQ=255)

                                                                              tttCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAagag                                                                    <  1:8650589/149‑1 (MQ=255)

                                                                                   gATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCt                                                               <  1:2009115/149‑1 (MQ=255)

                                                                                      aGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAAtt                                                                                          >  1:4013032/1‑119 (MQ=255)

                                                                                      aGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTgg                                                            >  2:9545452/1‑149 (MQ=255)

                                                                                      aGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTgg                                                            >  1:10372385/1‑149 (MQ=255)

                                                                                      aGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTgg                                                            <  2:192570/149‑1 (MQ=255)

                                                                                       gCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGt                                                           <  2:8262836/149‑1 (MQ=255)

                                                                                       gCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGt                                                           >  1:8048194/1‑149 (MQ=255)

                                                                                        ccTTTTTTTTTCATTTTAGAAACAAGTAGTTGAGTGGGATTGCTTTTGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTa                                                          >  2:9754257/1‑149 (MQ=255)

                                                                                        ccTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTa                                                          <  1:4675893/149‑1 (MQ=255)

                                                                                          tttttGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGt                                                        >  2:5187590/1‑149 (MQ=255)

                                                                                          tttttGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGt                                                        <  1:10157193/149‑1 (MQ=255)

                                                                                              tGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAgg                                                    >  2:5990614/1‑149 (MQ=255)

                                                                                                 aTCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGAtt                                                 >  2:1638050/1‑149 (MQ=255)

                                                                                                 aTCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGAtt                                                 <  1:7400254/149‑1 (MQ=255)

                                                                                                 aTCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGAtt                                                 >  1:7032151/1‑149 (MQ=255)

                                                                                                 aTCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGAtt                                                 <  2:1741359/149‑1 (MQ=255)

                                                                                                  tCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTa                                                <  2:3087045/149‑1 (MQ=255)

                                                                                                  tCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGACCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTa                                                <  1:6286634/149‑1 (MQ=255)

                                                                                                   cATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTaa                                               >  2:8090119/1‑149 (MQ=255)

                                                                                                   cATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTaa                                               >  1:7180873/1‑149 (MQ=255)

                                                                                                       tgAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATc                                           <  2:7209633/149‑1 (MQ=255)

                                                                                                       tgAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATc                                           >  2:2485156/1‑149 (MQ=255)

                                                                                                         agaAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAg                                         >  2:9244728/1‑149 (MQ=255)

                                                                                                           aaaCAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGtt                                       >  1:10009078/1‑149 (MQ=255)

                                                                                                             aCAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTc                                     >  1:9494510/1‑149 (MQ=255)

                                                                                                              cAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCa                                    <  1:2162929/149‑1 (MQ=255)

                                                                                                               aaGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCAt                                   >  1:1715599/1‑149 (MQ=255)

                                                                                                                        aGTGGGAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAAtc                          >  1:376732/1‑149 (MQ=255)

                                                                                                                           gggAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAAtcctc                       >  2:8824112/1‑149 (MQ=255)

                                                                                                                           gggAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAAtcctc                       <  2:6220512/149‑1 (MQ=255)

                                                                                                                           gggAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAAtcctc                       >  2:2028313/1‑149 (MQ=255)

                                                                                                                            ggAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGTTTAATATCAGTTTCATCTAATAAtcctcc                      <  2:6446707/149‑1 (MQ=255)

                                                                                                                            ggAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAAtcctcc                      >  2:8943480/1‑149 (MQ=255)

                                                                                                                             gAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATcctcct                     >  2:3810642/1‑149 (MQ=255)

                                                                                                                             gAGTGCGTTGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATcctcct                     <  1:2819019/149‑1 (MQ=255)

                                                                                                                                tGCGTTGGTTTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAGTCACGTCCAAGTAGAATGAGGGGACTTTCTTTGGCTAAATTGCTAGGCGCAGCTTGGTAGTCGGGATTAATTTCAGGTTCATCTAGGCCTCCTCCTaaa                  <  2:1176607/149‑1 (MQ=255)

                                                                                                                                     tGGTCTCTTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTaa             <  1:1591038/149‑1 (MQ=255)

                                                                                                                                         ctctTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACt         >  1:8939852/1‑149 (MQ=255)

                                                                                                                                         ctctTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACt         >  2:10214412/1‑149 (MQ=255)

                                                                                                                                           ctTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTGc       >  2:5657018/1‑149 (MQ=255)

                                                                                                                                           ctTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTGc       >  2:5051574/1‑149 (MQ=255)

                                                                                                                                           ctTTTTAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTGc       <  2:2619070/149‑1 (MQ=255)

                                                                                                                                            tttttAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGGATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTGCa      <  2:2239250/149‑1 (MQ=255)

                                                                                                                                            tttttAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTGCa      >  2:3516024/1‑149 (MQ=255)

                                                                                                                                             ttttAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTGCAt     <  2:1850082/149‑1 (MQ=255)

                                                                                                                                              tttAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTGCATc    >  1:3390006/1‑149 (MQ=255)

                                                                                                                                              tttAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTGCATc    <  2:5373194/149‑1 (MQ=255)

                                                                                                                                               ttAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTGCATCa   <  1:6456159/149‑1 (MQ=255)

                                                                                                                                                tAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTGCATCaa  >  2:4765028/1‑149 (MQ=255)

                                                                                                                                                tAGA‑GAAAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTGCATCaa  >  1:8124290/1‑149 (MQ=255)

TTCAGTCAAAATTTCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGA‑G‑AAA‑AATATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTGCATCAA  >  AM406671/1210130‑1210421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`