BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	1,614,130	+T	coding (1149/1278 nt)	mleP ←	malate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	1,614,128	1	.	T	98.8%	3538.0 / 21.2	935	coding (1151/1278 nt)	mleP	malate transporter
Reads supporting (aligned to +/- strand): ref base . (7/4); new base T (488/431); total (498/437)
Fisher's exact test for biased strand distribution p-value = 5.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 981 total aligned reads displayed.

TTTTTTTAGGTTATGGTTATTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑G‑TTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAATTGCCATGTAAAT  >  AM406671/1613980‑1614271

 ttttttAGGTTATGGTTATTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑G‑t                                                                                                                                                <  1:8212882/149‑1 (MQ=255)

    tttAGGTTATGGTTATTTTAATAAAAGAATCGTATAAGAATTCCACCGAATGTGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑G‑tttc                                                                                                                                             >  2:2823144/1‑147 (MQ=255)

    tttAGGTTATGGTTATTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑G‑tttc                                                                                                                                             >  1:4885875/1‑147 (MQ=255)

    tttAGGTTATGGTTATTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑G‑tttc                                                                                                                                             <  1:2970010/149‑3 (MQ=255)

      tAGGTTATGGTTATTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑G‑tttcca                                                                                                                                           >  2:4237362/1‑145 (MQ=255)

         gttatggttatTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCAccc                                                                                                                                         <  1:8660508/149‑1 (MQ=255)

           tatggttatTTTAATAATAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCAt                                                                                                                                       <  2:8390167/149‑1 (MQ=255)

           tatggttatTTTAATAAAAGAATCGAATAAGAATTCCACGGAATATGAGGACAATAGAACCACAAAGTATATTAGCAATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATAGAGACAAAACAGCAATGTTACCTAT‑TTCCACCGAt                                                                                                                                       >  2:7043698/1‑149 (MQ=255)

            atggttatTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATa                                                                                                                                      <  2:7594665/149‑1 (MQ=255)

             tggttatTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAg                                                                                                                                     <  1:9512440/149‑1 (MQ=255)

             tggttatTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGAAATGTTACCT‑GTTTCCACCCATAg                                                                                                                                     >  2:9758461/1‑149 (MQ=255)

                ttatTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAat                                                                                                                                  <  2:2112058/149‑1 (MQ=255)

                 tatTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAatt                                                                                                                                 >  2:2305069/1‑149 (MQ=255)

                  atTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAatta                                                                                                                                >  2:8188975/1‑149 (MQ=255)

                  atTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAatta                                                                                                                                <  2:1205390/149‑1 (MQ=255)

                   ttttAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAattat                                                                                                                               >  2:3322287/1‑149 (MQ=255)

                   ttttAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAattat                                                                                                                               >  1:138395/1‑149 (MQ=255)

                   ttttAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAAACAGCAATATTACCT‑GTTTCCACCCATAGAattat                                                                                                                               >  2:2661482/1‑149 (MQ=255)

                    tttAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAattatt                                                                                                                              <  1:6559847/149‑1 (MQ=255)

                     ttAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAattatta                                                                                                                             >  1:1082091/1‑148 (MQ=255)

                     ttAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTg                                                                                                                             >  2:3608267/1‑149 (MQ=255)

                      taataaAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACAACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGa                                                                                                                            >  2:7199770/1‑149 (MQ=255)

                       aataaAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGAt                                                                                                                           <  1:7976278/149‑1 (MQ=255)

                       aataaAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCAGATGCAGACAAGACAGCAATGTTACCT‑GTTTTCACCCGTAGAATTATTGAt                                                                                                                           >  1:5096376/1‑149 (MQ=255)

                         taaAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCa                                                                                                                         >  1:1884241/1‑149 (MQ=255)

                            aaGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATAc                                                                                                                      >  1:9321450/1‑149 (MQ=255)

                             aGAATCGTATAAGAATACCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATAcc                                                                                                                     <  1:742232/149‑1 (MQ=255)

                                aTCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGc                                                                                                                  <  1:5201137/149‑1 (MQ=255)

                                aTCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGc                                                                                                                  >  2:4106006/1‑149 (MQ=255)

                                  cGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCAc                                                                                                                >  1:9808219/1‑149 (MQ=255)

                                      taAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAAt                                                                                                            >  2:3140811/1‑149 (MQ=255)

                                       aaGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATg                                                                                                           >  2:9465362/1‑149 (MQ=255)

                                        aGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATgg                                                                                                          <  1:1545600/149‑1 (MQ=255)

                                         gAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGc                                                                                                         <  1:5540135/149‑1 (MQ=255)

                                         gAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGc                                                                                                         <  2:10353207/149‑1 (MQ=255)

                                            ttCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGt                                                                                                      <  1:8763375/149‑1 (MQ=255)

                                            ttCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTAATCATACCTGCACCAATCGCAGt                                                                                                      >  1:5961076/1‑149 (MQ=255)

                                               cACCGAATAGGAGGAAAAGTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTc                                                                                                   <  2:140065/149‑1 (MQ=255)

                                                 ccGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGa                                                                                                 <  1:4377639/149‑1 (MQ=255)

                                                  cGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGAc                                                                                                <  1:3205432/149‑1 (MQ=255)

                                                   gAATATGAGGAAAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTTCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACt                                                                                               <  2:5355022/149‑1 (MQ=255)

                                                    aaTATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTg                                                                                              >  1:675639/1‑149 (MQ=255)

                                                    aaTATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTg                                                                                              >  2:9618601/1‑149 (MQ=255)

                                                     atatGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTgg                                                                                             >  2:8835995/1‑149 (MQ=255)

                                                         gAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATaa                                                                                         >  2:4349407/1‑149 (MQ=255)

                                                            gACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAgg                                                                                      >  2:9177487/1‑149 (MQ=255)

                                                             aCAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGc                                                                                     <  1:3802179/149‑1 (MQ=255)

                                                                aTTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCTTACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCaa                                                                                  >  1:7538096/1‑149 (MQ=255)

                                                                aTTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAATGGCCaa                                                                                  <  2:5098038/149‑1 (MQ=255)

                                                                  tGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAag                                                                                <  1:8408099/149‑1 (MQ=255)

                                                                     accacAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAgg                                                                             <  1:7903841/149‑1 (MQ=255)

                                                                       cacaGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCg                                                                           >  2:2526355/1‑149 (MQ=255)

                                                                       cacaGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCg                                                                           >  1:1295818/1‑149 (MQ=255)

                                                                       cacaGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCg                                                                           >  2:7312920/1‑149 (MQ=255)

                                                                           gagTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGcc                                                                       >  2:2984876/1‑149 (MQ=255)

                                                                            agTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCa                                                                      <  1:10208212/149‑1 (MQ=255)

                                                                             gTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCaa                                                                     >  1:1200953/1‑149 (MQ=255)

                                                                               cTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGa                                                                   >  1:725406/1‑149 (MQ=255)

                                                                                tATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGaa                                                                  >  2:4944199/1‑149 (MQ=255)

                                                                                 aTTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAAc                                                                 <  2:1912715/149‑1 (MQ=255)

                                                                                   tAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAGGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAGGGCCAAAGAGGCGAGCCAAGAACCa                                                               <  2:5735888/149‑1 (MQ=255)

                                                                                    aGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAAGGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCaa                                                              <  1:8979627/149‑1 (MQ=255)

                                                                                     gCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGa                                                                                                                            >  2:9248195/1‑86 (MQ=255)

                                                                                        aTCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAg                                                          <  1:1752112/149‑1 (MQ=255)

                                                                                         tCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGc                                                         >  1:2401925/1‑149 (MQ=255)

                                                                                             aGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGt                                                     <  1:2067015/149‑1 (MQ=255)

                                                                                              gCAAAAGCAATCATTTCCATTCGATCTGNTGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTc                                                    >  1:5852459/1‑149 (MQ=255)

                                                                                               cAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTcc                                                   >  1:2780969/1‑149 (MQ=255)

                                                                                                aaaaGCAATCATTTCCATGCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAGTTAGTGATCATACGTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTccc                                                  >  2:6313261/1‑149 (MQ=255)

                                                                                                 aaaGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCa                                                 <  1:5657188/149‑1 (MQ=255)

                                                                                                  aaGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCAt                                                <  2:8678482/149‑1 (MQ=255)

                                                                                                   aGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCAtt                                               <  1:10009577/149‑1 (MQ=255)

                                                                                                     cAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCa                                                               <  2:8982372/131‑1 (MQ=255)

                                                                                                      aaTCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTaca                                            >  2:6758074/1‑149 (MQ=255)

                                                                                                       aTCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTacaa                                           >  2:7911420/1‑149 (MQ=255)

                                                                                                            ttCCATTCGGTCTGATGCAGACCAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTacaacaaca                                      <  2:8552045/149‑1 (MQ=255)

                                                                                                            ttCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTacaacaaca                                      <  2:479406/149‑1 (MQ=255)

                                                                                                             tCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCGATGGCAGATTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAacac                                     <  2:6562156/149‑1 (MQ=255)

                                                                                                             tCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAacac                                     <  1:408220/149‑1 (MQ=255)

                                                                                                              ccATTCTATCGGAGCCCGACAAAACAACAAGGTTGCCT‑GCTTCCACCCATCGAACTATAGATCTTACCGCCACCAACGGAAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACt                                    <  2:3968304/149‑1 (MQ=255)

                                                                                                                aTTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTg                                  >  1:997104/1‑149 (MQ=255)

                                                                                                                 ttCGTTCTGACGCAGACAAGACAGCAATGTTACCTGG‑TTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCTACTGGATAAAGGCCAAAGAGTCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGt                                 <  2:5589561/149‑1 (MQ=255)

                                                                                                                   cGATCTGATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCa                               <  1:2194623/149‑1 (MQ=255)

                                                                                                                   cGACCTAATGCAAACAAGACGGCAATGTTACCT‑GTTTCCACCCATAAAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTAGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAATCCCATTACAACAACACTTGTCa                               <  1:6274520/149‑1 (MQ=255)

                                                                                                                         gATGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCaaa                         >  2:5974381/1‑149 (MQ=255)

                                                                                                                          aTGCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAg                        >  1:3360795/1‑149 (MQ=255)

                                                                                                                            gCAGACAAGACAGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTa                      >  2:10165194/1‑149 (MQ=255)

                                                                                                                              agacaagacaGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTaca                    <  1:10362063/149‑1 (MQ=255)

                                                                                                                               gacaagacaGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTacaa                   <  1:9641654/149‑1 (MQ=255)

                                                                                                                                acaagacaGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGAAAAATacaac                  >  1:667447/1‑149 (MQ=255)

                                                                                                                                 caagacaGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTacaaca                 >  1:611527/1‑149 (MQ=255)

                                                                                                                                     acaGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAAtt             >  1:9069700/1‑149 (MQ=255)

                                                                                                                                       aGCAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGTCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAACAAAGTACAACAAATTGc           >  2:6096855/1‑149 (MQ=255)

                                                                                                                                        gCAATGTTACCT‑GTTTGCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAATTGcc          <  1:7048320/149‑1 (MQ=255)

                                                                                                                                         cAATGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAATTGCCa         >  2:4565107/1‑149 (MQ=255)

                                                                                                                                          aaTGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAATTGCCAt        <  1:847320/149‑1 (MQ=255)

                                                                                                                                           aTGTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAATTGCCATg       <  1:4235556/149‑1 (MQ=255)

                                                                                                                                             gTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAATTGCCATGTa     <  1:458533/149‑1 (MQ=255)

                                                                                                                                             gTTACCT‑GTTTCCACCCATAGAATTATTGATCATACCTGAACAAATGGAAGATTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAATTGCCATGTa     >  2:1691205/1‑149 (MQ=255)

                                                                                                                                                aCCT‑GTTTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAATTGCCATGTAAAt  <  1:5414879/149‑1 (MQ=255)

TTTTTTTAGGTTATGGTTATTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCT‑G‑TTCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAATTGCCATGTAAAT  >  AM406671/1613980‑1614271

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`