Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 827,811 C→T intergenic (+153/‑32) uxuA → / → uxuT D‑mannonate hydrolase/Na‑galactoside symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM406671827,8110CT99.4% 4116.6 / NA 1084intergenic (+153/‑32)uxuA/uxuTD‑mannonate hydrolase/Na‑galactoside symporter
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (562/512);  minor base A (0/7);  total (562/522)
Fisher's exact test for biased strand distribution p-value = 5.76e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1096 total aligned reads displayed.

GTTTTATGAAGGAATCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGACAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCTGGAACAATATTGTT  >  AM406671/827665‑827956
                                                                                                                                                  |                                                                                                                                                 
gTTTTAAAAAAGAATCAACTAAAAAGAAAGTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAAAAAAAAAAATAAAAAAATTTGTCAATTTTCACTGATaa                                                                                                                                                 <  2:6110637/149‑1 (MQ=255)
  tttATGAAGGAATCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATaaaa                                                                                                                                               >  1:6193597/1‑149 (MQ=255)
   ttATGAAGGAATCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATaaaaa                                                                                                                                              <  1:10333801/149‑1 (MQ=255)
   ttATGAAGGAATCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATaaaaa                                                                                                                                              <  1:9120269/149‑1 (MQ=255)
    tATGAAGGAATCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAat                                                                                                                                             <  1:6525261/149‑1 (MQ=255)
    tATGAAGGAATCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATGTGTCAATTTTCACTGATGAAAat                                                                                                                                             >  2:4801527/1‑149 (MQ=255)
     aTGAAGGAATCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAAAAAAAAAAATAAAAAAATTTGTCAATTTTCACTGATAAAAata                                                                                                                                            <  2:7323297/149‑1 (MQ=255)
        aaGGAATCTATTTTAAAGAACTTTTTGTGGAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATaa                                                                                                                                         <  2:8119012/149‑1 (MQ=255)
          ggAATCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATaaaa                                                                                                                                       <  1:10126887/149‑1 (MQ=255)
           gAATCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATaaaaa                                                                                                                                      >  1:2147729/1‑149 (MQ=255)
             aTCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGAtaaaaatataaaaata                                                                                                                                    <  2:1319899/149‑1 (MQ=255)
              tCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAACGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAg                                                                                                                                   >  2:2114507/1‑149 (MQ=255)
                tATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAGTTTTCACTGATAAAAATATAAAAATAggg                                                                                                                                 >  1:5499067/1‑148 (MQ=255)
                tATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGga                                                                                                                                 <  1:4867897/149‑1 (MQ=255)
                 aTTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTATATGTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTATAAGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGgag                                                                                                                                >  2:5721798/1‑149 (MQ=255)
                 aTTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGgag                                                                                                                                <  2:5245151/149‑1 (MQ=255)
                  ttttAAAGAACTTTTTGTGTAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGgaga                                                                                                                               <  1:5144971/149‑1 (MQ=255)
                  ttttAAAGAACTTTTTCTGAAAAAATGTGCGGAGAGTGTTCTCCGCTAAATTTAGAACGACGGAGTGAGCGTTTCACAAAGTTCACAATTTAACAAAGTTGATAGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGgaga                                                                                                                               <  2:1541302/149‑1 (MQ=255)
                    ttAAAGAACTTTTTGTTAAAAAATGTGCGGAGACTTTTCTCCGCTAACTTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAATAATATAAAAATAGGAGAAt                                                                                                                             >  2:5064476/1‑149 (MQ=255)
                    ttAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAAt                                                                                                                             >  1:2295412/1‑149 (MQ=255)
                    ttAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAAt                                                                                                                             >  2:8336671/1‑149 (MQ=255)
                     tAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATc                                                                                                                            <  2:3272332/149‑1 (MQ=255)
                       aaGAACTTTTTGTGAACAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTg                                                                                                                          <  1:7061080/149‑1 (MQ=255)
                       aaGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCtt                                                                                                                          >  2:2488266/1‑148 (MQ=255)
                       aaGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTg                                                                                                                          <  1:3415464/149‑1 (MQ=255)
                        aGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGt                                                                                                                         >  1:10052137/1‑149 (MQ=255)
                        aGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGt                                                                                                                         <  1:8754243/149‑1 (MQ=255)
                          aaCTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGttt                                                                                                                       >  2:5360186/1‑149 (MQ=255)
                             tttttGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTaaa                                                                                                                    <  2:8540319/149‑1 (MQ=255)
                             tttttGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTaaa                                                                                                                    <  1:1313052/149‑1 (MQ=255)
                             tttttGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTaaa                                                                                                                    <  1:8636569/149‑1 (MQ=255)
                              ttttGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTaaaa                                                                                                                   <  1:2625854/149‑1 (MQ=255)
                              ttttGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTaaaa                                                                                                                   >  1:9869636/1‑149 (MQ=255)
                                ttGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAACTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATCAAAATATAAAAATAGGAGAATCTGTTTAAAATg                                                                                                                 >  2:4288140/1‑149 (MQ=255)
                                 tgtgAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATgg                                                                                                                >  1:417444/1‑149 (MQ=255)
                                  gtgAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATATAAATAGGAGAATCTGTTTAAAATGGc                                                                                                               >  1:6704208/1‑149 (MQ=255)
                                  gtgAAAAAATGTGCGGAGACTTTTCTCCGATAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGGTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTCTCACTGATAAAAATATAAAAATAGGCGAATCTGTTTAAAATGGc                                                                                                               >  1:1142721/1‑149 (MQ=255)
                                    gAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAg                                                                                                             >  2:6501812/1‑149 (MQ=255)
                                     aaaaaaTGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGa                                                                                                            <  1:4729442/149‑1 (MQ=255)
                                     aaaaaaTGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGa                                                                                                            >  2:10152083/1‑149 (MQ=255)
                                     aaaaaaTGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGa                                                                                                            >  2:3657540/1‑149 (MQ=255)
                                      aaaaaTGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGaa                                                                                                           <  2:4684551/149‑1 (MQ=255)
                                      aaaaaTGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAAAAAAAAAAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGaa                                                                                                           <  2:406344/149‑1 (MQ=255)
                                        aaaTGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGaaaa                                                                                                         >  1:5646785/1‑149 (MQ=255)
                                        aaaTGTGCCGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGaaaa                                                                                                         <  1:731427/149‑1 (MQ=255)
                                         aaTGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGGAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGaacaa                                                                                                        >  2:746188/1‑149 (MQ=255)
                                           tgtgAGGAGACTTTTCTCCGCTAAAATTAGAACAACCAAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAATGTATAAAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACa                                                                                                      <  2:8767874/149‑1 (MQ=255)
                                                  agaCATTACTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAAATTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGAAAAAAAAAAAAAAACt                                                                                               <  2:3783587/149‑1 (MQ=255)
                                                    aCTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTc                                                                                             >  1:10451650/1‑149 (MQ=255)
                                                       tttCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTg                                                                                          <  1:9491195/149‑1 (MQ=255)
                                                       tttCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTg                                                                                          >  1:3101725/1‑149 (MQ=255)
                                                          ctcCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGt                                                                                       >  1:3638176/1‑149 (MQ=255)
                                                          ctcCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGt                                                                                       <  2:7969250/149‑1 (MQ=255)
                                                            ccGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGttt                                                                                     >  2:8599893/1‑149 (MQ=255)
                                                              gCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTac                                                                                   >  1:7917740/1‑149 (MQ=255)
                                                              gCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTac                                                                                   >  2:1815167/1‑149 (MQ=255)
                                                                          acgacgGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGaa                                                                       <  2:7105399/149‑1 (MQ=255)
                                                                              cgGACTAAGCGTTTCACAAAGTTCACAACTTAACAAAGTTTATGGAAAAATTTGTCAATCTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAACTGGCAGAAAAACAAAAAACTTCGTGGGTTTACACCCCAAAAGAAATTa                                                                   >  2:2661500/1‑149 (MQ=255)
                                                                                gAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAAt                                                                 <  1:4340823/149‑1 (MQ=255)
                                                                                 aGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATa                                                                >  1:9672407/1‑149 (MQ=255)
                                                                                 aGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATa                                                                >  1:1870513/1‑149 (MQ=255)
                                                                                 aGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATa                                                                <  1:7435946/149‑1 (MQ=255)
                                                                                  gTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATAc                                                               >  1:1664924/1‑149 (MQ=255)
                                                                                   tAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACa                                                              <  1:3964181/149‑1 (MQ=255)
                                                                                    aaGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACaa                                                             >  1:8460742/1‑149 (MQ=255)
                                                                                      gCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAgg                                                           >  2:2237162/1‑149 (MQ=255)
                                                                                         tttCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGt                                                        >  2:9095646/1‑149 (MQ=255)
                                                                                              caAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGtct                                                   >  2:3541406/1‑149 (MQ=255)
                                                                                               aaaGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTATACACCAAAAGAAATTAATACAAGGCGTGGtctt                                                  >  2:9295083/1‑149 (MQ=255)
                                                                                                aaGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACATACCAAAAGAAATTAATACAAGGCGTGGtcttc                                                 >  1:9299956/1‑149 (MQ=255)
                                                                                                  gTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTcttctt                                               >  2:9750476/1‑149 (MQ=255)
                                                                                                   ttCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCttt                                              <  1:3251450/149‑1 (MQ=255)
                                                                                                          tttAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGCTAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCtt                                       >  2:6313777/1‑149 (MQ=255)
                                                                                                          tttAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCtt                                       <  2:6809492/149‑1 (MQ=255)
                                                                                                           ttAACAAAGTTTATGGAAAAATTTGTCGATTTTCACTGACAAAAATATAAAAGTAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTa                                      <  1:7583042/149‑1 (MQ=255)
                                                                                                           ttAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATAGCAAAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTa                                      <  2:1205627/149‑1 (MQ=255)
                                                                                                               cAAAGTTTATGGAAAAATTTGTCAATTTNCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCg                                  >  1:5765480/1‑149 (MQ=255)
                                                                                                                 aaGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGAt                                >  1:7752446/1‑149 (MQ=255)
                                                                                                                 aaGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGAt                                <  1:2716444/149‑1 (MQ=255)
                                                                                                                     ttATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTaa                            >  1:9987694/1‑149 (MQ=255)
                                                                                                                     ttATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTaa                            >  1:6824785/1‑149 (MQ=255)
                                                                                                                      tATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAAt                           <  2:6930964/149‑1 (MQ=255)
                                                                                                                        tGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATgg                         <  1:2388881/149‑1 (MQ=255)
                                                                                                                         ggAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATggg                        >  2:9481919/1‑149 (MQ=255)
                                                                                                                         ggAAAAATTTGTCAAGTTTCACTGATAAAAATATAAAAATAGGAGAATATGTTTAAAATGGCAGAAAAACAAAAAACTGCGAGGGATTACACACCAAAATAAAATAATACAAAGCGAGGACTTCTTTACGGCTTAAACGATTTAATggg                        >  2:1703105/1‑149 (MQ=255)
                                                                                                                          gAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGAAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGc                       <  2:3329427/149‑1 (MQ=255)
                                                                                                                           aaaaaTTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCg                      >  1:5323196/1‑149 (MQ=255)
                                                                                                                                   gTCAATTTTCACTGATAAAAATATAAAAATAGGGGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCTgg              <  1:4631517/149‑1 (MQ=255)
                                                                                                                                   gTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAGCCGATTTAATGGGCGGAGGCTgg              >  1:5949755/1‑149 (MQ=255)
                                                                                                                                       aTTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCTGGAACa          >  1:3774550/1‑149 (MQ=255)
                                                                                                                                       aTTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCTGGAACa          <  1:2913380/149‑1 (MQ=255)
                                                                                                                                        ttttCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCTGGAACaa         >  2:10446693/1‑149 (MQ=255)
                                                                                                                                         tttCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCTGGAACAat        <  1:8964878/149‑1 (MQ=255)
                                                                                                                                         tttCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCTGGAACAat        >  1:8300551/1‑149 (MQ=255)
                                                                                                                                         tttCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCTGGAACAat        >  2:4227136/1‑149 (MQ=255)
                                                                                                                                          ttCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCTGGAACAata       <  2:7767497/149‑1 (MQ=255)
                                                                                                                                          ttCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCTGGAACAata       >  1:8082295/1‑149 (MQ=255)
                                                                                                                                            cACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGGGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCTGGAACAATAtt     >  2:4976763/1‑149 (MQ=255)
                                                                                                                                              cTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCTGGAACAATATTGt   >  2:4451833/1‑149 (MQ=255)
                                                                                                                                               tGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCCGGAACAATATTGtt  >  2:2991857/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GTTTTATGAAGGAATCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGACAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCTGGAACAATATTGTT  >  AM406671/827665‑827956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑