Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 912,618 T→A L46Q (CTA→CAA)  llmg_0944 → putative secreted protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM406671912,6180TA99.8% 3308.8 / NA 858L46Q (CTA→CAA) llmg_0944putative secreted protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (468/387);  minor base G (0/2);  total (469/389)
Fisher's exact test for biased strand distribution p-value = 2.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 864 total aligned reads displayed.

AGGAAAATAAATGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCTAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAATTTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATT  >  AM406671/912472‑912762
                                                                                                                                                  |                                                                                                                                                
     aataaatGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGTTATTGTTATGATAGGTCGACACGTTATTTCTCGTCAAACTGat                                                                                                                                           >  2:2461277/1‑149 (MQ=255)
     aataaatGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGat                                                                                                                                           >  1:3726490/1‑149 (MQ=255)
      ataaatGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGata                                                                                                                                          <  1:8125587/149‑1 (MQ=255)
        aaatGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGataat                                                                                                                                        >  2:9252719/1‑149 (MQ=255)
        aaatGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGataat                                                                                                                                        >  2:280515/1‑149 (MQ=255)
         aatGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGataata                                                                                                                                       >  2:8918087/1‑149 (MQ=255)
          atGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTATCCTTTAATATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAatat                                                                                                                                      >  2:6571010/1‑149 (MQ=255)
              aTTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTgca                                                                                                                                  <  2:2718748/149‑1 (MQ=255)
               ttCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTgcag                                                                                                                                 >  1:9488066/1‑149 (MQ=255)
                 cTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGCCACGTTATTTCTAGTCAAACAGATAATATTgcagca                                                                                                                               <  2:6851389/149‑1 (MQ=255)
                   agaaaaagaaaaACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCaaa                                                                                                                             >  1:854012/1‑149 (MQ=255)
                    gaaaaagaaaaaCAGAGAAGAAAGGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGATACGTTATTTCTAGTCAAACAGATAATATTGCAGCaaaa                                                                                                                            >  2:1650099/1‑149 (MQ=255)
                       aaagaaaaaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAAttt                                                                                                                         >  1:7488728/1‑149 (MQ=255)
                        aagaaaaaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCTAAATTta                                                                                                                        >  2:5146168/1‑149 (MQ=255)
                        aagaaaaaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTta                                                                                                                        >  2:1105321/1‑149 (MQ=255)
                         agaaaaaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTtat                                                                                                                       <  2:5942410/149‑1 (MQ=255)
                          gaaaaaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATCACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACCCGATATTTCTAGTCAAACAGATAATATTGCAGCAAAATTtata                                                                                                                      >  2:7565666/1‑149 (MQ=255)
                            aaaaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATAcc                                                                                                                    >  1:10133891/1‑149 (MQ=255)
                              aaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAg                                                                                                                  >  2:2536979/1‑149 (MQ=255)
                               aCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGa                                                                                                                 <  2:10023754/149‑1 (MQ=255)
                               aCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGa                                                                                                                 <  2:396084/149‑1 (MQ=255)
                               aCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGa                                                                                                                 <  2:768885/149‑1 (MQ=255)
                                  gagaAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCaaaa                                                                                                                            <  1:2464584/135‑1 (MQ=255)
                                      agaaACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCaa                                                                                                          <  2:8469567/149‑1 (MQ=255)
                                      agaaACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCaa                                                                                                          <  2:6688227/149‑1 (MQ=255)
                                       gaaACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCaaa                                                                                                         <  2:4851032/149‑1 (MQ=255)
                                           cGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACtt                                                                                                     <  2:132810/149‑1 (MQ=255)
                                           cGCAGAGGGGGTAAAGTATTTGCAATACTACTCTCCCTCATTATATTCCTGGCTCTGATTGTTTGTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCCCAAAACtt                                                                                                     >  2:4291046/1‑149 (MQ=255)
                                            gCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTa                                                                                                    <  2:7191306/149‑1 (MQ=255)
                                               gagGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACg                                                                                                 >  2:147595/1‑149 (MQ=255)
                                                agGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACgg                                                                                                >  1:9592427/1‑149 (MQ=255)
                                                agGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACgg                                                                                                >  2:3535458/1‑149 (MQ=255)
                                                agGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACgg                                                                                                <  1:1627630/149‑1 (MQ=255)
                                                 gggggTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGa                                                                                               <  2:10409711/149‑1 (MQ=255)
                                                 gggggTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGa                                                                                               >  1:6982175/1‑149 (MQ=255)
                                                   gggTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATg                                                                                             >  1:5510968/1‑149 (MQ=255)
                                                     gTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCa                                                                                           <  1:3509361/149‑1 (MQ=255)
                                                       aaaGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTTTTATTTTTTTGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACTAGAATCTCAAAACTTAACGGATGCAGc                                                                                         >  2:33933/1‑149 (MQ=255)
                                                       aaaGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGc                                                                                         <  2:4108136/149‑1 (MQ=255)
                                                       aaaGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGc                                                                                         >  1:5352994/1‑149 (MQ=255)
                                                         aGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGTTATTGTATTGATAGGTCGATACGTTATTTCTAGTCAAACAGATATTATTGCAGCAATATTTATACCAGAATCTCAAAATTTAACGGATGCAGCgg                                                                                       >  2:8004464/1‑149 (MQ=255)
                                                          gTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGa                                                                                      >  2:8094676/1‑149 (MQ=255)
                                                           tATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGAt                                                                                     >  1:6014577/1‑149 (MQ=255)
                                                           tATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGAt                                                                                     >  1:2712816/1‑149 (MQ=255)
                                                             tttGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCa                                                                                   >  1:5069099/1‑149 (MQ=255)
                                                              ttGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCaa                                                                                  >  2:9084401/1‑149 (MQ=255)
                                                               tGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCaaa                                                                                 <  1:9209231/149‑1 (MQ=255)
                                                                    tatTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGt                                                                            <  1:3291408/149‑1 (MQ=255)
                                                                      ttACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTc                                                                          >  2:5035439/1‑149 (MQ=255)
                                                                       tACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCg                                                                         >  1:1994889/1‑149 (MQ=255)
                                                                        aCTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCgg                                                                        <  1:4313372/149‑1 (MQ=255)
                                                                        aCTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCgg                                                                        >  1:4071289/1‑149 (MQ=255)
                                                                        aCTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCgg                                                                        <  1:7280005/149‑1 (MQ=255)
                                                                         ctctCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTGGGc                                                                       >  1:571961/1‑149 (MQ=255)
                                                                           ctcCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTa                                                                     <  1:227396/149‑1 (MQ=255)
                                                                           ctcCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGGTAAACCGATAATATTGCAGCAAAATTTATACCAGTATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTa                                                                     >  2:7428408/1‑149 (MQ=255)
                                                                            tcCCTTATTATATTTCTGGCACTGCTTGTTTTTGGTAGTGTAATAAGAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAACATTTATACCAGAATCTCTAAACTTAACGGATGCTGCTGATCAAATTGGTTCGGCTAc                                                                    <  2:10230248/149‑1 (MQ=255)
                                                                              ccTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTc                                                                  <  1:9381352/149‑1 (MQ=255)
                                                                              ccTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGAAGCGGATCAAATTGGTTCGGCTACTc                                                                  >  1:5861316/1‑149 (MQ=255)
                                                                                ttattaTATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCg                                                                <  1:2955590/149‑1 (MQ=255)
                                                                                  attatATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTc                                                              >  1:6649314/1‑149 (MQ=255)
                                                                                    tataTTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATATGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAg                                                            >  2:9845686/1‑149 (MQ=255)
                                                                                    tataTTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAg                                                            >  2:3761061/1‑149 (MQ=255)
                                                                                    tataTTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAg                                                            <  1:3873176/149‑1 (MQ=255)
                                                                                     atatTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGc                                                           >  1:8650389/1‑149 (MQ=255)
                                                                                      tatTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCt                                                          >  1:5281381/1‑149 (MQ=255)
                                                                                           cTGGCTCTGATTGTTTTTGGTATTGTTATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTgc                                                     >  2:7306764/1‑149 (MQ=255)
                                                                                            tGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTgca                                                    >  2:3135840/1‑149 (MQ=255)
                                                                                               ctctGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTgcaagc                                                 >  1:7169102/1‑149 (MQ=255)
                                                                                                tctGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTgcaagca                                                <  1:797594/149‑1 (MQ=255)
                                                                                                 ctGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTgcaagcaa                                               >  2:8247707/1‑149 (MQ=255)
                                                                                                 ctGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTgcaagcaa                                               <  2:2035048/149‑1 (MQ=255)
                                                                                                    aTTGTTTTTGTTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAatt                                            <  1:1121889/149‑1 (MQ=255)
                                                                                                        tttttGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTCCTCCGTCAGCTATTGCAAGCAAATTAAAt                                        <  2:6471741/149‑1 (MQ=255)
                                                                                                            tGGTATTGTAATGGTAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGTAGCAAAATTTATACCAGAATCCCAAAACTTAAGGGATGGAGCGGATCAAGTTGGTTAGGCTACTCCGTCAGCGATTGCAAGCAAATTAAATgaga                                    >  2:9565384/1‑149 (MQ=255)
                                                                                                             ggTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGaa                                   <  1:4903656/149‑1 (MQ=255)
                                                                                                              gTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAAt                                  >  2:940201/1‑149 (MQ=255)
                                                                                                              gTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAAt                                  <  2:5450854/149‑1 (MQ=255)
                                                                                                              gTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAAt                                  >  2:8794292/1‑149 (MQ=255)
                                                                                                                aTTGTAATGATAGGAGGACACGTTATTTCTAGTCAAACAGATAATATTGCAACAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTc                                >  2:2160798/1‑149 (MQ=255)
                                                                                                                 ttGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCg                               >  1:180549/1‑149 (MQ=255)
                                                                                                                    tAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGAt                            >  2:6152129/1‑149 (MQ=255)
                                                                                                                     aaTGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATa                           >  2:5674662/1‑149 (MQ=255)
                                                                                                                       tGATAGGACGACACGTTATTTCTAGTCAAACAGATAAGATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGTGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATAcc                         <  2:6976007/149‑1 (MQ=255)
                                                                                                                         aTAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCAt                       <  1:4636990/149‑1 (MQ=255)
                                                                                                                          tAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATc                      >  2:1856107/1‑149 (MQ=255)
                                                                                                                            ggACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCaa                    <  2:55696/149‑1 (MQ=255)
                                                                                                                              acgacACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAaga                  <  2:2358651/149‑1 (MQ=255)
                                                                                                                                  cacGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCa              >  1:141806/1‑149 (MQ=255)
                                                                                                                                  cacGTTATTTCTAGGCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCa              <  1:9762803/149‑1 (MQ=255)
                                                                                                                                     gTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAAtc           >  1:10043786/1‑149 (MQ=255)
                                                                                                                                       tATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAAtctc         <  1:7666931/149‑1 (MQ=255)
                                                                                                                                       tATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAAtctc         <  1:10336571/149‑1 (MQ=255)
                                                                                                                                        aTTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCa        <  1:8883959/149‑1 (MQ=255)
                                                                                                                                          ttCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAgg      <  1:3079137/149‑1 (MQ=255)
                                                                                                                                           tCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAggg     >  1:6367962/1‑149 (MQ=255)
                                                                                                                                             tAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGAt   <  2:7801983/149‑1 (MQ=255)
                                                                                                                                             tAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGAt   >  2:4446585/1‑149 (MQ=255)
                                                                                                                                              aGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGAtt  >  1:6809251/1‑149 (MQ=255)
                                                                                                                                              aGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGAtt  <  1:909919/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
AGGAAAATAAATGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCTAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAATTTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATT  >  AM406671/912472‑912762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑