Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 912,718 T→A N79K (AAT→AAA)  llmg_0944 → putative secreted protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM406671912,7180TA99.9% 4052.7 / NA 1023N79K (AAT→AAA) llmg_0944putative secreted protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base A (527/495);  total (528/495)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1028 total aligned reads displayed.

ATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCTAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAATTTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTAGAATCTTCTAAGCTTAATAAAGCAACCGTTGGTGCCCTTTTACTTGCAA  >  AM406671/912572‑912863
                                                                                                                                                  |                                                                                                                                                 
  tGTTTTTGGTATTGTAATGATAGTACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTaa                                                                                                                                               >  2:274485/1‑149 (MQ=255)
    tttttGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTCCTCCGTCAGCTATTGCAAGCAAATTAAAt                                                                                                                                             <  2:6471741/149‑1 (MQ=255)
    tttttGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAAt                                                                                                                                             >  1:1581333/1‑149 (MQ=255)
     ttttGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATg                                                                                                                                            <  1:213165/149‑1 (MQ=255)
      tttGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATga                                                                                                                                           <  2:7392164/149‑1 (MQ=255)
       ttGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATgag                                                                                                                                          >  2:7707427/1‑149 (MQ=255)
         ggTATTGTAATGATAGGTCGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGaa                                                                                                                                        <  2:5268581/149‑1 (MQ=255)
         ggTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATACTGCAGCAAAATTTATACCAGAATATCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGaa                                                                                                                                        <  1:1791341/149‑1 (MQ=255)
              tGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCgg                                                                                                                                   <  2:63785/149‑1 (MQ=255)
               gTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAAAATTCGGa                                                                                                                                  >  2:3463914/1‑149 (MQ=255)
                  aTGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATAc                                                                                                                               <  2:9372971/149‑1 (MQ=255)
                   tGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATAcc                                                                                                                              >  2:5451892/1‑149 (MQ=255)
                     aTAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCAt                                                                                                                            <  1:2495153/149‑1 (MQ=255)
                      tAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATc                                                                                                                           >  2:1856107/1‑149 (MQ=255)
                       aGGACGACACGTTATTTCTAGTCAAACAGATAATAGTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCa                                                                                                                          <  2:6149318/149‑1 (MQ=255)
                        ggACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCaa                                                                                                                         >  1:7372553/1‑149 (MQ=255)
                        ggACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCaa                                                                                                                         <  1:1696363/149‑1 (MQ=255)
                            gacACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGt                                                                                                                     <  2:4007010/149‑1 (MQ=255)
                                  ttATTTCTAGTCAAACAGATAATATTGCAGCAACATTTATACCAGAATCTCAAAACTTAACGGATGCCGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCCAtcc                                                                                                               >  2:7916926/1‑148 (MQ=255)
                                  ttATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGagtcagtca                                                                                                               >  2:8692118/1‑148 (MQ=255)
                                    aTTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCa                                                                                                             >  2:361954/1‑149 (MQ=255)
                                    aTTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCa                                                                                                             >  1:4974849/1‑149 (MQ=255)
                                      ttCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCACGAGGCAATCTCAgg                                                                                                           >  1:3871485/1‑149 (MQ=255)
                                      ttCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATAACATCAAGAGTCAATCTCAgg                                                                                                           >  2:8493038/1‑149 (MQ=255)
                                        cTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGa                                                                                                         >  1:8795493/1‑149 (MQ=255)
                                         tAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGAt                                                                                                        <  1:77710/149‑1 (MQ=255)
                                          aGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGAtt                                                                                                       <  1:909919/149‑1 (MQ=255)
                                             caaaCAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAAt                                                                                                    >  1:4571801/3‑149 (MQ=255)
                                             caaaCAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAAt                                                                                                    >  1:1340462/3‑149 (MQ=255)
                                                aCAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCaa                                                                                                 >  1:5515279/1‑149 (MQ=255)
                                                aCAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCaa                                                                                                 <  2:7612283/149‑1 (MQ=255)
                                                  aGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAAtt                                                                                               <  2:8805027/149‑1 (MQ=255)
                                                   gATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTc                                                                                              <  1:7128380/149‑1 (MQ=255)
                                                    ataataTTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCg                                                                                             <  1:2348504/149‑1 (MQ=255)
                                                      aataTTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGc                                                                                           <  1:2962270/149‑1 (MQ=255)
                                                       atatTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCa                                                                                          >  2:9200333/1‑149 (MQ=255)
                                                       atatTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGAGGCAGCGGATGAAATTGGTCCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATTAATTCggaa                                                                                          >  2:7183983/1‑147 (MQ=255)
                                                         atTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCaaa                                                                                        >  2:1405899/1‑149 (MQ=255)
                                                         atTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCaaa                                                                                        <  2:9798093/149‑1 (MQ=255)
                                                          ttGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCaaaa                                                                                       >  2:8103448/1‑149 (MQ=255)
                                                          ttGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCaaaa                                                                                       >  2:9662191/1‑149 (MQ=255)
                                                               gcaAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGcgac                                                                                  >  1:5303483/1‑149 (MQ=255)
                                                               gcaAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGcgac                                                                                  <  2:8332515/149‑1 (MQ=255)
                                                               gcaAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGcgac                                                                                  >  2:2441692/1‑149 (MQ=255)
                                                                caAAAGTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGcgacg                                                                                 >  2:4350590/1‑149 (MQ=255)
                                                                  aaaTTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAg                                                                               <  1:6873534/149‑1 (MQ=255)
                                                                   aaTTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGt                                                                              <  2:6950552/149‑1 (MQ=255)
                                                                    aTTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTa                                                                             <  1:9939624/149‑1 (MQ=255)
                                                                     tttATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTAc                                                                            <  2:5599190/149‑1 (MQ=255)
                                                                      ttATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACt                                                                           <  2:2914663/149‑1 (MQ=255)
                                                                       tataCCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTg                                                                          >  2:1703594/1‑149 (MQ=255)
                                                                        ataCCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGa                                                                         >  1:1949892/1‑149 (MQ=255)
                                                                         taCCAGAATCTCAAAACTTGACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGAc                                                                        >  2:9440413/1‑149 (MQ=255)
                                                                         taCCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGAc                                                                        >  1:10317418/1‑149 (MQ=255)
                                                                            cAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCa                                                                     >  1:8932311/1‑149 (MQ=255)
                                                                            cAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCa                                                                     <  2:446565/149‑1 (MQ=255)
                                                                             aGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCaa                                                                    <  1:3731418/149‑1 (MQ=255)
                                                                               aaTCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAATTTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAACGACGAGTACTGACTCAAAg                                                                  >  2:3853568/1‑149 (MQ=255)
                                                                               aaTCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCAt                                                                                                                            <  1:8571329/91‑1 (MQ=255)
                                                                                  ctcAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTg                                                               >  1:9617637/1‑149 (MQ=255)
                                                                                    cAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGAc                                                             >  1:6279659/1‑149 (MQ=255)
                                                                                    cAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGAc                                                             >  1:7261456/1‑149 (MQ=255)
                                                                                           tAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGCGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACt                                                      >  2:2261750/1‑149 (MQ=255)
                                                                                            aaCGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACtt                                                     >  1:6081226/1‑149 (MQ=255)
                                                                                            aaCGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACtt                                                     >  1:8064221/1‑149 (MQ=255)
                                                                                             aCGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGCACTGACTCAAAGCTGACCTATACttt                                                    >  1:6457571/1‑149 (MQ=255)
                                                                                              cGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTa                                                   <  1:6750308/149‑1 (MQ=255)
                                                                                              cGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTa                                                   >  1:10000586/1‑149 (MQ=255)
                                                                                                 aTGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAGGCTGACCTATACTTTAGaa                                                >  1:5131676/1‑149 (MQ=255)
                                                                                                 aTGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTAGaa                                                >  1:3617588/1‑149 (MQ=255)
                                                                                                    cAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTAGAAtct                                             <  1:3364884/149‑1 (MQ=255)
                                                                                                      gCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTAGAAtcttc                                           >  1:4289313/1‑149 (MQ=255)
                                                                                                      gCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTAGAAtcttc                                           >  2:10146544/1‑149 (MQ=255)
                                                                                                       cGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTAGAAtcttct                                          >  2:6822962/1‑149 (MQ=255)
                                                                                                       cGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTAGAAtcttct                                          <  2:5944709/149‑1 (MQ=255)
                                                                                                          aTCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTAGAATCTTCTAAg                                       <  2:3238860/149‑1 (MQ=255)
                                                                                                          aTCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTAGAATCTTCTAAg                                       >  2:4614573/1‑149 (MQ=255)
                                                                                                            cAAATTGCTGCGGCTACGCCGTCAGCTGTTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTAGAATCTTCTAAGCt                                     <  2:1144493/149‑1 (MQ=255)
                                                                                                              aaTTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTAGAATCTTCTAAGCTta                                   <  1:9344077/149‑1 (MQ=255)
                                                                                                              aaTTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTAGAATCTTCTAAGCTta                                   >  1:8306296/1‑149 (MQ=255)
                                                                                                                ttGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTAGAATCTTCTAAGCTtaat                                 <  2:2056412/149‑1 (MQ=255)
                                                                                                                   gTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTAGAATCTTCTAAGCTTAATaaa                              <  1:10193635/149‑1 (MQ=255)
                                                                                                                   gTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTAGAATCTTCTAAGCTTAATaaa                              >  1:4816986/1‑149 (MQ=255)
                                                                                                                    ttCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTAGAATCTTCTAAGCTTAATAAAg                             >  1:661006/1‑149 (MQ=255)
                                                                                                                       ggCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTAGAATCTTCTAAGCTTAATAAAGCaa                          >  2:9012776/1‑149 (MQ=255)
                                                                                                                        gCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTAGAATCTTCTAAGCTTAATAAAGCAAc                         <  2:6663705/149‑1 (MQ=255)
                                                                                                                            cTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTAGAATCTTCTAAGCTTAATAAAGCAACCGtt                     >  2:10346942/1‑149 (MQ=255)
                                                                                                                             tCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTAGAATCTTCTAAGCTTAATAAAGCAACCGTTg                    <  1:5932619/149‑1 (MQ=255)
                                                                                                                             tCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTAGAATCTTCTAAGCTTAATAAAGCAACCGTTg                    <  2:9269868/149‑1 (MQ=255)
                                                                                                                              ccGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTAGAATCTTCTAAGCTTAATAAAGCAACCGTTgg                   >  1:9692619/1‑149 (MQ=255)
                                                                                                                                gTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTAGAATCTTCTAAGCTTAATAAAGCAACCGTTGGTg                 <  1:9206926/149‑1 (MQ=255)
                                                                                                                                 tCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTAGAATCTTCTAAGCTTAATAAAGCAACCGTTGGTGc                >  2:4997735/1‑149 (MQ=255)
                                                                                                                                  cAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTAGAATCTTCTAAGCTTAATAAAGCAACCGTTGGTGcc               <  1:7557365/149‑1 (MQ=255)
                                                                                                                                  cAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTAGAATCTTCTAAGCTTAATAAAGCAACCGTTGGTGcc               <  1:5696205/149‑1 (MQ=255)
                                                                                                                                        ttGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTAGAATCTTCTAAGCTTAATAAAGCAACCGTTGGTGCCCTTTTa         >  1:2749434/1‑149 (MQ=255)
                                                                                                                                        ttGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTAGAATCTTCTAAGCTTAATAAAGCAACCGTTGGTGCCCTTTTa         >  2:8220173/1‑149 (MQ=255)
                                                                                                                                         tGCAACCAAATTAAATGAGAATTCTGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACCCAAAGCTGACCTATACTTTAGAATCTTCTAAGCTTAATAAAGCAACCGTTGGTGCCCTTTTAc        >  2:2582022/1‑149 (MQ=255)
                                                                                                                                          gcaagcaaATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTAGAATCTTCTAAGCTTAATAAAGCAACCGTTGGTGCCCTTTTACt       >  1:10425541/1‑149 (MQ=255)
                                                                                                                                             agcaaATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTAGAATCTTCTAAGCTTAATAAAGCAACCGTTGGTGCCCTTTTACTTGc    >  1:4070491/1‑149 (MQ=255)
                                                                                                                                              gcaaATTAAATGAGAATTCGGATACCATCAAGAGGCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTAGAATCTTCTAAGCTTAATAAAGCAACCGTTGGTGCCCTTTTACTTGCa   <  1:3235105/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCTAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAATTTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTAATCAATTCGGCAAAAGCGACGAGTACTGACTCAAAGCTGACCTATACTTTAGAATCTTCTAAGCTTAATAAAGCAACCGTTGGTGCCCTTTTACTTGCAA  >  AM406671/912572‑912863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑