Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 295,026 T→C S40P (TCG→CCG)  rimI2 → Ribosomal‑protein‑alanine acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM406671295,0260TC99.6% 5230.2 / NA 1293S40P (TCG→CCG) rimI2Ribosomal‑protein‑alanine acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (3/2);  new base C (747/537);  total (754/539)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1304 total aligned reads displayed.

GCAATTTGGATGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGTCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGACCAATATCGCCATTCA  >  AM406671/294880‑295171
                                                                                                                                                  |                                                                                                                                                 
      tGGATGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTgg                                                                                                                                           <  2:7197317/149‑1 (MQ=255)
       ggATGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACATTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGt                                                                                                                                          <  2:6550434/149‑1 (MQ=255)
        gATGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCtt                                                                                                                                             <  1:1125000/145‑1 (MQ=255)
        gATGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTc                                                                                                                                         >  2:5439045/1‑149 (MQ=255)
        gATGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTc                                                                                                                                         >  2:1766614/1‑149 (MQ=255)
         agggTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAAGAAGTCGAAAGACAAGAATACTTTTCCACCTGTGGCAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCGTATGGACATCCGCTTTGGTca                                                                                                                                        >  2:5929579/3‑149 (MQ=255)
          tGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTcac                                                                                                                                       <  2:9853832/149‑1 (MQ=255)
          tGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTcac                                                                                                                                       >  2:9731972/1‑149 (MQ=255)
           ggTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTcaca                                                                                                                                      >  1:8444646/1‑149 (MQ=255)
           ggTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTcaca                                                                                                                                      <  1:3880468/149‑1 (MQ=255)
           ggTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTcaca                                                                                                                                      >  1:3690736/1‑149 (MQ=255)
             tCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAg                                                                                                                                    >  2:10071917/1‑149 (MQ=255)
                 aGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTta                                                                                                                                <  1:10278164/149‑1 (MQ=255)
                 aGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATAGTTATGAACTTTTATCAGTGGCTTATGGACAGTCGCCTTGGTCACAAGTTta                                                                                                                                >  1:5087988/1‑149 (MQ=255)
                   aaTTAAAAAAATGAATTTGAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTtata                                                                                                                              <  1:9947146/149‑1 (MQ=255)
                   aaTTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTtata                                                                                                                              <  2:6144064/149‑1 (MQ=255)
                    aTTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTGATGGACAGCCGCCTTGGTCACAAGTTtatat                                                                                                                             <  1:5290600/149‑1 (MQ=255)
                     ttAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTtatata                                                                                                                            >  1:4907512/1‑149 (MQ=255)
                      tAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTatatat                                                                                                                           >  1:3395204/1‑149 (MQ=255)
                      tAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTatatat                                                                                                                           >  2:881435/1‑149 (MQ=255)
                         aaaaaTGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGa                                                                                                                        <  1:9613459/149‑1 (MQ=255)
                            aaTGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTg                                                                                                                     >  1:2241647/1‑149 (MQ=255)
                            aaTGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTg                                                                                                                     >  1:5711027/1‑149 (MQ=255)
                            aaTGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTg                                                                                                                     <  2:6857026/149‑1 (MQ=255)
                             aTGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGa                                                                                                                    <  1:351765/149‑1 (MQ=255)
                               gAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGAtt                                                                                                                  <  1:7915646/149‑1 (MQ=255)
                               gAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGAtt                                                                                                                  >  1:6102470/1‑149 (MQ=255)
                                  tttAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAg                                                                                                               >  1:1782098/1‑149 (MQ=255)
                                   ttAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGGCAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGt                                                                                                              <  2:7078712/149‑1 (MQ=255)
                                     aaaaCGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATGTTTATGAACTTTTATCAGTGGCTTATGGGCAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTag                                                                                                            <  2:4210242/149‑2 (MQ=255)
                                        aCGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACg                                                                                                         >  2:7349051/1‑149 (MQ=255)
                                          gtctgtctTACTCTGAAAATAAGTCGCAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCCCAAGTTCATATATCGAGTGATTTACTAAACGaa                                                                                                       >  2:637097/1‑149 (MQ=255)
                                          gtctgtctTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGaa                                                                                                       <  2:3084166/149‑1 (MQ=255)
                                          gtctgtctTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGaa                                                                                                       <  1:1492396/149‑1 (MQ=255)
                                            ctgtctTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGaaaa                                                                                                     <  2:2742393/149‑1 (MQ=255)
                                               tctTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTc                                                                                                  <  1:9410821/149‑1 (MQ=255)
                                                 ttACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCgg                                                                                                >  2:5556838/1‑149 (MQ=255)
                                                 ttACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCgg                                                                                                >  1:7749259/1‑149 (MQ=255)
                                                     tctGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATa                                                                                            <  1:7149841/149‑1 (MQ=255)
                                                     tctGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATa                                                                                            <  1:2356665/149‑1 (MQ=255)
                                                      ctGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATAc                                                                                           >  1:4080152/1‑149 (MQ=255)
                                                      ctGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATAc                                                                                           >  1:2544707/1‑149 (MQ=255)
                                                       tGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACt                                                                                          >  1:5914179/1‑149 (MQ=255)
                                                        gAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACtt                                                                                         <  1:6828817/149‑1 (MQ=255)
                                                        gAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACtt                                                                                         <  2:1327732/149‑1 (MQ=255)
                                                         aaaaTAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACttt                                                                                        <  1:9780595/149‑1 (MQ=255)
                                                          aaaTAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACtttt                                                                                       >  1:10430562/1‑149 (MQ=255)
                                                          aaaTAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACtttt                                                                                       >  2:2345593/1‑149 (MQ=255)
                                                            aTAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTa                                                                                     >  1:1930776/1‑149 (MQ=255)
                                                                 tCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTaga                                                                                >  1:4403560/1‑149 (MQ=255)
                                                                   gAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTagaag                                                                              <  1:8999938/149‑1 (MQ=255)
                                                                    aaaGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTagaaga                                                                             <  1:7466702/149‑1 (MQ=255)
                                                                     aaGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAAt                                                                                                    >  1:7037434/1‑125 (MQ=255)
                                                                      aGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTagaagaag                                                                           >  2:4853371/1‑149 (MQ=255)
                                                                      aGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTagaagaag                                                                           >  2:7778843/1‑149 (MQ=255)
                                                                       gACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTagaagaaga                                                                          >  2:521498/1‑149 (MQ=255)
                                                                       gACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTagaagaaga                                                                          >  2:9267034/1‑149 (MQ=255)
                                                                       gACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTagaagaaga                                                                          <  1:3105704/149‑1 (MQ=255)
                                                                       gACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTagaagaaga                                                                          <  1:4612130/149‑1 (MQ=255)
                                                                       gACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTacaagaaga                                                                          >  2:3555944/1‑149 (MQ=255)
                                                                       gACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGCCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTagaagaaga                                                                          >  1:6298470/1‑149 (MQ=255)
                                                                        aCAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGAt                                                                         >  2:7510129/1‑149 (MQ=255)
                                                                         cAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATg                                                                        <  1:2943469/149‑1 (MQ=255)
                                                                         cAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATg                                                                        <  2:2903331/149‑1 (MQ=255)
                                                                           aaaaTACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTAGGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGCAGAAGATGAg                                                                      >  2:8581349/1‑149 (MQ=255)
                                                                                aCTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCaatt                                                                 <  1:5516862/149‑1 (MQ=255)
                                                                                 cTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCaatta                                                                >  2:4404355/1‑149 (MQ=255)
                                                                                  ttttCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCaattaa                                                               >  2:8153153/1‑149 (MQ=255)
                                                                                       cACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGt                                                          <  2:9030328/149‑1 (MQ=255)
                                                                                       cACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGt                                                          <  1:129424/149‑1 (MQ=255)
                                                                                           tgtgGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTctgc                                                      >  2:4102177/1‑149 (MQ=255)
                                                                                           tgtgGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTctgc                                                      >  1:8812666/1‑149 (MQ=255)
                                                                                            gtgGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTctgct                                                     >  1:4761969/1‑149 (MQ=255)
                                                                                               gAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGc                                                  <  1:4231091/149‑1 (MQ=255)
                                                                                                  aaGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCAt                                               >  2:7954704/1‑149 (MQ=255)
                                                                                                  aaGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCAt                                               >  1:2063074/1‑149 (MQ=255)
                                                                                                     ttATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTc                                            <  2:2509101/149‑1 (MQ=255)
                                                                                                     ttATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATAGCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTc                                            >  1:6572658/1‑149 (MQ=255)
                                                                                                      tATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCa                                           <  2:8433426/149‑1 (MQ=255)
                                                                                                      tATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCa                                           >  2:7626253/1‑149 (MQ=255)
                                                                                                      tATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCa                                           >  2:2219744/1‑149 (MQ=255)
                                                                                                         cAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCaata                                        >  1:10001440/1‑149 (MQ=255)
                                                                                                           gTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCaataat                                      <  2:2112401/149‑1 (MQ=255)
                                                                                                           gTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCaataat                                      >  2:10387779/1‑149 (MQ=255)
                                                                                                              aaTATTTATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAatgat                                   >  2:4616139/1‑149 (MQ=255)
                                                                                                                  tttATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATgatggat                               <  1:8150874/149‑1 (MQ=255)
                                                                                                                  tttATGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATgatggat                               <  2:9460389/149‑1 (MQ=255)
                                                                                                                     aTGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGCGCAATTACTTGGTCTGCTGGCCATTTCACTAATGATGGATGaa                            >  1:3252241/1‑149 (MQ=255)
                                                                                                                      tGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAAt                           >  2:10194283/1‑149 (MQ=255)
                                                                                                                      tGAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAAt                           <  2:5775547/149‑1 (MQ=255)
                                                                                                                       gAACTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAAtt                          <  2:153887/149‑1 (MQ=255)
                                                                                                                        aaCTTTTATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTa                         >  1:9267012/1‑149 (MQ=255)
                                                                                                                            tttATCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAg                     >  1:7631891/1‑149 (MQ=255)
                                                                                                                               aTCAGTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGa                  >  1:2761851/1‑149 (MQ=255)
                                                                                                                                   gTGGCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGACCaa              >  2:327264/1‑149 (MQ=255)
                                                                                                                                     ggCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAATTGACCAata            <  2:502836/149‑1 (MQ=255)
                                                                                                                                     ggCTTATGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGACCAata            >  1:8681038/1‑149 (MQ=255)
                                                                                                                                          aTGGACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGACCAATATCGcc       >  2:3830306/1‑149 (MQ=255)
                                                                                                                                             gACAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGACCAATATCGCCAtt    <  1:8566787/149‑1 (MQ=255)
                                                                                                                                               cAGCCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGACCAATATCGCCATTCa  <  2:1131009/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GCAATTTGGATGGTCAAAGAATTAAAAAAATGAATTTAAAACGTCTGTCTTACTCTGAAAATAAGTCGAAAGACAAAAATACTTTTCCACCTGTGGAAAAGTTATCAGTCAATATTTATGAACTTTTATCAGTGGCTTATGGACAGTCGCCTTGGTCACAAGTTTATATATCGAGTGATTTAGTAAACGAAAATTCGGAATACTTTTTACTAGAAGAAGATGAGCAATTAATTGGTCTGCTGGCCATTTCAATAATGATGGATGAATTAGAAGTGACCAATATCGCCATTCA  >  AM406671/294880‑295171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑