Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 1,277,684 T→A N231I (AAT→ATT)  llmg_1306 ← conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM4066711,277,6840TA99.8% 3314.2 / NA 860N231I (AAT→ATT) llmg_1306conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base A (364/493);  total (364/496)
Fisher's exact test for biased strand distribution p-value = 5.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 860 total aligned reads displayed.

CTGAATTTTGTAAAATTTTACTCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCATTAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTCCTTTCAGGC  >  AM406671/1277538‑1277828
                                                                                                                                                  |                                                                                                                                                
 tGAATTTTGTAAAATTTTACTCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATaa                                                                                                                                               >  1:3742071/1‑149 (MQ=255)
     ttttGTAAAATTTTACTCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGa                                                                                                                                           <  1:8989732/149‑1 (MQ=255)
      tttGTAAAATTTTACTCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGaa                                                                                                                                          <  1:6651598/149‑1 (MQ=255)
      tttGTAAAATTTTACTCGTATCTAACCATCCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGaa                                                                                                                                          <  2:10245421/149‑1 (MQ=255)
       ttGTAAAATTTTACTCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAAc                                                                                                                                         <  1:420223/149‑1 (MQ=255)
          tAAAATTTTACTCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGa                                                                                                                                      <  2:3830895/149‑1 (MQ=255)
           aaaaTTTTACTCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGaa                                                                                                                                     >  2:1990342/1‑149 (MQ=255)
            aaaTTTTACTCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGaaa                                                                                                                                    >  1:93260/1‑149 (MQ=255)
                 ttACTCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGtt                                                                                                                               >  1:5172526/1‑149 (MQ=255)
                  tACTCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGa                                                                                                                                           >  2:8010332/1‑136 (MQ=255)
                   aCTCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTc                                                                                                                             >  2:2432664/1‑149 (MQ=255)
                     tCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCt                                                                                                                           >  2:6882841/1‑149 (MQ=255)
                     tCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCt                                                                                                                           <  1:5875989/149‑1 (MQ=255)
                      cGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTa                                                                                                                          <  1:4782475/149‑1 (MQ=255)
                        tATCGAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTAAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGa                                                                                                                        <  2:562015/149‑1 (MQ=255)
                         aTCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGAt                                                                                                                       <  2:2862734/149‑1 (MQ=255)
                         aTCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGAt                                                                                                                       >  2:658956/1‑149 (MQ=255)
                         aTCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGAt                                                                                                                       <  2:5904976/149‑1 (MQ=255)
                          tCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGAtg                                                                                                                      <  1:9696552/149‑1 (MQ=255)
                            tAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGAtgtg                                                                                                                    <  2:2210938/149‑1 (MQ=255)
                             aaCCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTc                                                                                                                             >  2:547796/1‑139 (MQ=255)
                             aaCCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGc                                                                                                                   <  2:8505430/149‑1 (MQ=255)
                              aCCATGCAATTTGTCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCt                                                                                                                  >  2:4293034/1‑149 (MQ=255)
                              aCCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCt                                                                                                                  <  2:8635169/149‑1 (MQ=255)
                               ccATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTc                                                                                                                 <  2:6162905/149‑1 (MQ=255)
                                 aTGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCt                                                                                                               <  1:10428408/149‑1 (MQ=255)
                                  tGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCtt                                                                                                              <  2:8989152/149‑1 (MQ=255)
                                       tttGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTAttt                                                                                                         <  1:6866064/149‑1 (MQ=255)
                                          ggCTCCAATCTCCTACAGCAAAACCTGTTTCAGTGGCACCAGTTACTGTTCGCCCAGAATTTAACAGCGGACTATTAGCTGCGATCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTACCTAGATTTGCCCCTTTATATTCa                                                                                                      >  2:7757474/1‑149 (MQ=255)
                                          ggCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCa                                                                                                      <  2:3709240/149‑1 (MQ=255)
                                          ggCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCa                                                                                                      <  2:6709083/149‑1 (MQ=255)
                                          ggCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCa                                                                                                      <  1:193277/149‑1 (MQ=255)
                                           gCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAg                                                                                                     >  1:4970906/1‑149 (MQ=255)
                                              ccAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGt                                                                                                  <  1:3007996/149‑1 (MQ=255)
                                                  tctcCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGCGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTAt                                                                                              >  2:3540604/1‑149 (MQ=255)
                                                   ctcCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTTGATGTGCTCCTTTATTTTCAGGGTCTAtt                                                                                             <  2:6442351/149‑1 (MQ=255)
                                                   ctcCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTAtt                                                                                             >  2:5775596/1‑149 (MQ=255)
                                                    tcCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTAttt                                                                                            >  2:7860576/1‑149 (MQ=255)
                                                     ccTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTc                                                                                           >  1:4000635/1‑149 (MQ=255)
                                                      cTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTcc                                                                                          <  1:8575193/149‑1 (MQ=255)
                                                      cTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTcc                                                                                          <  1:5703/149‑1 (MQ=255)
                                                      cTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTcc                                                                                          <  1:4358925/149‑1 (MQ=255)
                                                      cTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTcc                                                                                          >  1:3176347/1‑149 (MQ=255)
                                                       tACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTccc                                                                                         <  1:7987849/149‑1 (MQ=255)
                                                       tACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTccc                                                                                         <  2:4495255/149‑1 (MQ=255)
                                                        aCAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCa                                                                                        <  1:9802563/149‑1 (MQ=255)
                                                           tcAAAACCTTCTTCAGTGGAAAAAGTTACTGTTCGTCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGcc                                                                                     <  2:322778/148‑1 (MQ=255)
                                                           gCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGcc                                                                                     >  2:7231002/1‑149 (MQ=255)
                                                             aaaaCCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTa                                                                                   <  2:9724730/149‑1 (MQ=255)
                                                              aaaCCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTaa                                                                                  <  2:606073/149‑1 (MQ=255)
                                                               aaCCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGAAGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAAt                                                                                 >  1:6410044/1‑149 (MQ=255)
                                                                aCCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATc                                                                                <  1:2052116/149‑1 (MQ=255)
                                                                 ccTGCTTCAGTGGAAAAAGTTACTGTGCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCt                                                                               <  2:3373516/149‑1 (MQ=255)
                                                                    gCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATg                                                                            <  1:10022609/149‑1 (MQ=255)
                                                                    gCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATg                                                                            >  1:7428509/1‑149 (MQ=255)
                                                                        cAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTa                                                                        <  1:5558691/149‑1 (MQ=255)
                                                                             gAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTatcatc                                                                   >  1:6165293/1‑149 (MQ=255)
                                                                               aaaaGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTatcatcat                                                                 >  1:8307868/1‑149 (MQ=255)
                                                                                aaaGTTACTGTTCGCCCAGAGTTTAAAAGCGGACTATTGGCTGCGGTCGGTGTCTTTGTTGTCTCAATAAAAGAGCCGCAAGGGTTTCCTAGAGGTACCCCTTTATTTTCAGGGTCTATTTCCCAGCCCAATCTATGATTatcatcatc                                                                <  2:9254563/149‑1 (MQ=255)
                                                                                   gTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCAtt                                                             >  2:5207705/1‑149 (MQ=255)
                                                                                   gTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCAtt                                                             >  1:1788215/1‑149 (MQ=255)
                                                                                    ttACTGCTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTACTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCGATGATTATCATCATCATTa                                                            >  2:5096513/1‑149 (MQ=255)
                                                                                     tACTGTTCGCCCAGGATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTAc                                                           <  1:7792561/149‑1 (MQ=255)
                                                                                      aCTGTTCGCCCAGAACTTAAAAGCGGACTGTTTGCTGCGGTCGGTGACGTCGTTGTCTCAATAAAAGAACCGAAAGCGTTTCCTAGATGTGCTCCTTTATTCTCAAGGACTATTTCCCAGCCTACTCTATGATTATCATCATCATAACt                                                          >  2:4704624/1‑149 (MQ=255)
                                                                                       cTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACtt                                                         <  2:10416028/149‑1 (MQ=255)
                                                                                          ttCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTTTTGTCTCCATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTttgt                                                      <  2:398749/149‑1 (MQ=255)
                                                                                          ttCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGGTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTttgt                                                      >  1:4544726/1‑149 (MQ=255)
                                                                                            cGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTttgttg                                                    >  1:4643277/1‑149 (MQ=255)
                                                                                            cGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTttgttg                                                    <  1:9157112/149‑1 (MQ=255)
                                                                                             gCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTga                                                   <  1:4451625/149‑1 (MQ=255)
                                                                                                cAGAATTTCAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTgagga                                                <  1:6012059/149‑1 (MQ=255)
                                                                                                cAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTgagga                                                >  1:10209498/1‑149 (MQ=255)
                                                                                                cAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTgagga                                                <  1:1236267/149‑1 (MQ=255)
                                                                                                cAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTgagga                                                >  2:4139218/1‑149 (MQ=255)
                                                                                                 aGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTgaggag                                               >  1:8778062/1‑149 (MQ=255)
                                                                                                  gAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGt                                              <  2:7611292/149‑1 (MQ=255)
                                                                                                     tttAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTAtt                                           <  2:1611346/149‑1 (MQ=255)
                                                                                                      ttAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTc                                          >  1:2813336/1‑149 (MQ=255)
                                                                                                          aaGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAAt                                      <  1:7072481/149‑1 (MQ=255)
                                                                                                           aGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATa                                     >  2:7485739/1‑149 (MQ=255)
                                                                                                             cGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAgg                                   <  1:3451466/149‑1 (MQ=255)
                                                                                                                 cTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATc                               >  1:9548750/1‑149 (MQ=255)
                                                                                                                 cTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATc                               <  2:8169478/149‑1 (MQ=255)
                                                                                                                 cTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATc                               >  1:5301299/1‑149 (MQ=255)
                                                                                                                    ttAGCTGCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCaaa                            >  1:1595412/1‑149 (MQ=255)
                                                                                                                     tAGCTGCGGTCGGTGTCGTTGTTGTCTCNATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAAt                           >  1:5771411/1‑149 (MQ=255)
                                                                                                                        cTGCGGTCGGTGTCGTTGTTGTCTCAATTAAAGAACCGATAGGGTTTCCTAGATGTGCGCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTta                        <  1:7558671/149‑1 (MQ=255)
                                                                                                                        cTGCGGACGGTTTCGTGTGTGTCTAATTAAAAGAGCCGAAACGATTTCCTAGAGATGCGCCTTGATTTTCAAGTTCTATTGCCCAGCCTAATCTATGGTTATCATCATCATTACTTTGTTGAGAAGTATTCCAATAGGTATCAAATTta                        <  2:1357775/149‑1 (MQ=255)
                                                                                                                          gCGGTCGGTGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTtata                      <  2:2694586/149‑1 (MQ=255)
                                                                                                                            ggtcggtGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAAc                    <  1:721945/149‑1 (MQ=255)
                                                                                                                               tggtGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCaa                 <  2:9610054/148‑1 (MQ=255)
                                                                                                                                ggtGTCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAAt                <  2:8724150/149‑1 (MQ=255)
                                                                                                                                    tCGTTGTTGTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCt            >  2:1862476/1‑149 (MQ=255)
                                                                                                                                       ttgttgTCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTCCt         <  2:3024865/149‑1 (MQ=255)
                                                                                                                                        tgttgtCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGAAGTATTCCAATAAGTATCAAATTTATAACAAATAGCTCCtt        >  2:1010310/1‑149 (MQ=255)
                                                                                                                                         gttgtCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTCCttt       <  2:9443781/149‑1 (MQ=255)
                                                                                                                                         gttgtCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTCCttt       <  1:3860172/149‑1 (MQ=255)
                                                                                                                                          ttgtCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACAAATAGCTCCTTTc      >  2:8357597/1‑149 (MQ=255)
                                                                                                                                            gtCTCAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTCCTTTCAg    <  1:2432664/149‑1 (MQ=255)
                                                                                                                                              ctcAATAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTCCTTTCAGGc  >  1:1522902/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CTGAATTTTGTAAAATTTTACTCGTATCTAACCATGCAATTTGGCTCCAATCTCCTACAGCAAAACCTGCTTCAGTGGAAAAAGTTACTGTTCGCCCAGAATTTAAAAGCGGACTATTAGCTGCGGTCGGTGTCGTTGTTGTCTCATTAAAAGAACCGAAAGGGTTTCCTAGATGTGCTCCTTTATTTTCAGGGTCTATTTCCCAGCCTAATCTATGATTATCATCATCATTACTTTGTTGAGGAGTATTCCAATAGGTATCAAATTTATAACCAATAGCTCCTTTCAGGC  >  AM406671/1277538‑1277828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑