BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	1,492,365	T→A	L262L (CTA→CTT)	kinE ←	sensor kinase protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	1,492,365	0	T	A	99.7%	3376.6 / NA	914	L262L (CTA→CTT)	kinE	sensor kinase protein
Reads supporting (aligned to +/- strand): ref base T (2/1); new base A (457/452); total (461/453)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 914 total aligned reads displayed.

GCAGGTTGAATTTCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGATAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATTCTCAATTTTTTGATTTAATC  >  AM406671/1492219‑1492510

 cAGGTTGAATTTCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGc                                                                                                                                                <  1:5562631/149‑1 (MQ=255)

 cAGGTTGAATTTCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGc                                                                                                                                                <  2:3172179/149‑1 (MQ=255)

 cAGGTTGAATTTCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTGTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGc                                                                                                                                                >  1:4680397/1‑149 (MQ=255)

  aGGTTGAATTTCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCGTCGTCAATATAAGAAAggg                                                                                                                                               >  2:3339784/1‑147 (MQ=255)

   ggTTGAATTTCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTTCAAATGATTTCCCCGAGAAATTGTCAGTATATTTTCAATGAGCCCTTTCCTTCTCGGGGACTCTTGATCAATATAAGAAAGCGa                                                                                                                                              >  2:3524165/1‑149 (MQ=255)

    gTTGAATTTCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTTTCACTAAATTTTCAATTAGCCCTTTCATTCTCAGGGACTATGCATCAACATAATAAATCGAg                                                                                                                                             >  2:8246089/1‑149 (MQ=255)

    gTTGAATTTCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAg                                                                                                                                             >  2:4249243/1‑149 (MQ=255)

    gTTGAATTTCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTACAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATACTCAGGGACACTTAATCAATATAATAAAGCGAg                                                                                                                                             >  2:5110611/1‑149 (MQ=255)

         aTTTCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGtcatc                                                                                                                                        >  1:6005867/1‑149 (MQ=255)

          tttCACTGACCACTTTGTCAGTAAATTCTGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCTCCGAGAAATTGTCAGTAAATTTTCAATAAGCACTTTCATTCTCAGGGATTCTTGATCAATGTAACATAGCTATttatca                                                                                                                                       >  2:6119307/1‑149 (MQ=255)

          tttCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGtcatca                                                                                                                                       <  1:7278030/149‑1 (MQ=255)

           ttCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCaa                                                                                                                                      >  1:3203655/1‑149 (MQ=255)

            tCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCaat                                                                                                                                     >  1:8610239/1‑149 (MQ=255)

            tCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCaat                                                                                                                                     >  2:3968285/1‑149 (MQ=255)

              aCTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCaataa                                                                                                                                   <  1:1338006/149‑1 (MQ=255)

              aCTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGCCATCaataa                                                                                                                                   >  1:9133280/1‑149 (MQ=255)

               cTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCaataat                                                                                                                                  <  1:8870096/149‑1 (MQ=255)

                 gACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGATCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCaataatat                                                                                                                                >  2:8705782/1‑147 (MQ=255)

                  aCCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTc                                                                                                                               <  1:7743507/149‑1 (MQ=255)

                     aCTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCggg                                                                                                                            <  2:8096410/149‑1 (MQ=255)

                     aCTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTACAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTTATTCTCAGGGACTCTTCATCAATATAAGAAAGCGGGTCATCAAGAATTTCtgg                                                                                                                            >  2:6034037/1‑149 (MQ=255)

                      cTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGa                                                                                                                           <  2:1403281/149‑1 (MQ=255)

                       tttGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGAt                                                                                                                          <  1:10015257/149‑1 (MQ=255)

                        ttGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATg                                                                                                                         <  2:2303551/149‑1 (MQ=255)

                           tCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTc                                                                                                                      <  1:5292673/149‑1 (MQ=255)

                           tCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTc                                                                                                                      <  1:973737/149‑1 (MQ=255)

                           tCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTc                                                                                                                      <  2:3678442/149‑1 (MQ=255)

                            cAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCa                                                                                                                     <  2:10191815/149‑1 (MQ=255)

                             aGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCAt                                                                                                                    <  2:6770336/149‑1 (MQ=255)

                              gTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATg                                                                                                                   <  1:9864503/149‑1 (MQ=255)

                              gTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATg                                                                                                                   <  1:6586427/149‑1 (MQ=255)

                               tacaTTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGc                                                                                                                  <  2:6983534/146‑1 (MQ=255)

                               tAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGc                                                                                                                  <  1:578369/149‑1 (MQ=255)

                                aaaTTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTCCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGcc                                                                                                                 <  1:3547557/149‑1 (MQ=255)

                                     cAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCAtctt                                                                                                            <  1:3996414/149‑1 (MQ=255)

                                       gTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCAtcttct                                                                                                          <  1:7899458/149‑1 (MQ=255)

                                        tCAAGTTAAATTTTTCTTTATTTACTTCTAAATGATTTCACCGCGAAATTGTCAGTAACTTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATcttctt                                                                                                         <  1:7556696/149‑1 (MQ=255)

                                        tCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGATTCTTTATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATcttctt                                                                                                         >  2:4732580/1‑149 (MQ=255)

                                        tCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATcttctt                                                                                                         <  2:9363195/149‑1 (MQ=255)

                                         cAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCGATCTTCttt                                                                                                        >  2:8991814/1‑149 (MQ=255)

                                         cAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCttt                                                                                                        >  1:1139225/1‑149 (MQ=255)

                                          aaGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCtttt                                                                                                       <  1:9548153/149‑1 (MQ=255)

                                            nttAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTta                                                                                                     >  1:5209318/2‑149 (MQ=255)

                                            gTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTta                                                                                                     >  1:7163543/1‑149 (MQ=255)

                                             ttAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTtaa                                                                                                    >  1:1839656/1‑149 (MQ=255)

                                                actttttCTTTATTTACTTCCAAATGATTTCCCCGAGAAACTGTCAGTAAATTTTCAATGAGCCCTTTCATTCGCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTtaataa                                                                                                 <  2:7571033/147‑1 (MQ=255)

                                                aaTTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAATAAATTTTCAATGAGTACTTTCTTTGTCAGGTACTCTTCATTAATTTTTTAAAATGTGTCATCTATAATTTTTGGATCAACATGCCATCTTCTTTTttataa                                                                                                 >  2:2085293/1‑149 (MQ=255)

                                                 aTTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATaaa                                                                                                <  2:7918929/149‑1 (MQ=255)

                                                   ttttCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGt                                                                                              <  1:16726/149‑1 (MQ=255)

                                                    ttttttttttTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTTATCAATATAAGAATGTGAGTCATAAATAATTTCGAGATGCTCATACCATCTTCTTTTTAATAAATTg                                                                                             >  2:2887163/1‑149 (MQ=255)

                                                    tttCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTg                                                                                             <  2:7658073/149‑1 (MQ=255)

                                                    tttCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTg                                                                                             >  1:383787/1‑149 (MQ=255)

                                                       ttttatttaCTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGTGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACa                                                                                          <  2:9577621/148‑1 (MQ=255)

                                                          tatttaCTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGt                                                                                       <  1:8433720/149‑1 (MQ=255)

                                                            tttaCTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTcc                                                                                     <  2:4416595/149‑1 (MQ=255)

                                                                cTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACGTGTCCACGa                                                                                 <  1:5412674/149‑1 (MQ=255)

                                                                cTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGa                                                                                 <  2:3830169/149‑1 (MQ=255)

                                                                   ccAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAAtt                                                                              >  2:8451229/1‑149 (MQ=255)

                                                                      aaTGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTgca                                                                           <  2:1104155/149‑1 (MQ=255)

                                                                      aaTGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCAGTCACAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGTGATGCTAATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTtca                                                                           >  2:10303084/1‑149 (MQ=255)

                                                                       aTGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGACATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTgcag                                                                          >  1:2772812/1‑149 (MQ=255)

                                                                         gATTTCCCCGAGAAATTGTCAGTACATTTTCAATGAGCCCCTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAACTTCGGGATGCTCATGCCATCTTCTTTTTAACAACGTGACATGTCCACGAATTgcagca                                                                        >  2:5532270/1‑149 (MQ=255)

                                                                           tttCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAAc                                                                      >  2:2449047/1‑149 (MQ=255)

                                                                            ttCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGTACTCTTCATCAAAATAAGTAAGCGAGTTATCAATAATTTCGGGATGCTAAAGGCATCTTCTTATTAATAAAGTGACATGTCGAAGACTCGCAACAACg                                                                     >  2:6199195/1‑149 (MQ=255)

                                                                             tCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACgg                                                                    <  1:3006963/149‑1 (MQ=255)

                                                                              ccccGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACggg                                                                   <  1:4907724/149‑1 (MQ=255)

                                                                                    gaAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATACTTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGACCt                                                             >  2:5252251/1‑149 (MQ=255)

                                                                                    gaAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCt                                                             <  1:4180944/149‑1 (MQ=255)

                                                                                     aaaTTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTc                                                            <  1:239001/149‑1 (MQ=255)

                                                                                     aaaTTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTc                                                            >  1:4303961/1‑149 (MQ=255)

                                                                                        ttGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGt                                                         <  2:3402401/149‑1 (MQ=255)

                                                                                         tGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGtt                                                        >  1:8268349/1‑149 (MQ=255)

                                                                                              gTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCAtgt                                                   <  2:9685987/149‑1 (MQ=255)

                                                                                                aaaTTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGa                                                 >  2:7375153/1‑149 (MQ=255)

                                                                                                 aaTTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGaa                                                <  2:7793343/149‑1 (MQ=255)

                                                                                                 aaTTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGaa                                                >  1:8107708/1‑149 (MQ=255)

                                                                                                   ttttCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGc                                              >  1:4522344/1‑149 (MQ=255)

                                                                                                     ttCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCAt                                            <  1:10418446/149‑1 (MQ=255)

                                                                                                     ttCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCAt                                            >  2:10392206/1‑149 (MQ=255)

                                                                                                      tCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCAGCTTCTTTTTAAGAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATc                                           <  1:2165708/149‑1 (MQ=255)

                                                                                                       cAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATcc                                          >  2:5352537/1‑149 (MQ=255)

                                                                                                       cAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATcc                                          >  2:9458317/1‑149 (MQ=255)

                                                                                                          tGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGaa                                       <  1:2572258/149‑1 (MQ=255)

                                                                                                           aaGCCCTTTCATTCTCAGTGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAAGTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGaaa                                      <  2:7149746/148‑1 (MQ=255)

                                                                                                              cccTTTCATTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGaaacaa                                   <  2:5778777/149‑1 (MQ=255)

                                                                                                                     aTTCTCAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAActgctg                            >  2:4963866/1‑149 (MQ=255)

                                                                                                                        ctcAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTc                         <  2:9155179/149‑1 (MQ=255)

                                                                                                                         tcAGGGACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCa                        <  1:9357004/149‑1 (MQ=255)

                                                                                                                            gggACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATtc                     <  1:6808085/149‑1 (MQ=255)

                                                                                                                             ggACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATtct                    >  1:1491501/1‑149 (MQ=255)

                                                                                                                             ggACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATtct                    >  1:10209905/1‑149 (MQ=255)

                                                                                                                             ggACTCTTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAAGAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTAATGTGAAGCATCCGACACAAACTGCTGCTCATtct                    >  2:6600453/1‑149 (MQ=255)

                                                                                                                                ctctTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATTCTCaa                 <  2:2634533/149‑1 (MQ=255)

                                                                                                                                ctctTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATTCTCaa                 <  2:4134059/149‑1 (MQ=255)

                                                                                                                                 gctccatcaATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTACATCAACGGCCGTCCGCAGTTCATGTGAAGCATCCAAAACAAACTGCTGCTCATTCTCact                >  2:166952/5‑147 (MQ=255)

                                                                                                                                  ctTCATCAATATAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATTCTCAAtt               <  2:6397296/149‑1 (MQ=255)

                                                                                                                                          atatAAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATTCTCAATTTTTTGAtt       >  2:465096/1‑149 (MQ=255)

                                                                                                                                             taAGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATTCTCAATTTTTTGATTTaa    <  2:8891569/149‑1 (MQ=255)

                                                                                                                                              aaGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATTCTCAATTTTTTGATTTAAt   >  1:706406/1‑149 (MQ=255)

                                                                                                                                              aaGAAAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATTCTCAATTTTTTGATTTAAt   <  1:6181545/149‑1 (MQ=255)

GCAGGTTGAATTTCACTGACCACTTTGTCAGTAAATTCAGTCAAGTTAAATTTTTCTTTATTTACTTCCAAATGATTTCCCCGAGAAATTGTCAGTAAATTTTCAATGAGCCCTTTCATTCTCAGGGACTCTTCATCAATATAAGATAGCGAGTCATCAATAATTTCGGGATGCTCATGCCATCTTCTTTTTAATAAAGTGACATGTCCACGAATTGCAGCAACGGGCGTCCTCAGTTCATGTGAAGCATCCGAAACAAACTGCTGCTCATTCTCAATTTTTTGATTTAATC  >  AM406671/1492219‑1492510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`