Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 1,588,193 T→G Q297P (CAA→CCA)  ugd ← UDP‑glucose 6‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM4066711,588,1930TG99.0% 3853.8 / NA 942Q297P (CAA→CCA) ugdUDP‑glucose 6‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (9/0);  new base G (476/455);  total (485/457)
Fisher's exact test for biased strand distribution p-value = 3.90e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 954 total aligned reads displayed.

CATAATCTTTCAAATCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTTGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGTATTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACC  >  AM406671/1588047‑1588338
                                                                                                                                                  |                                                                                                                                                 
 aTAATCTTTCAAATCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGgag                                                                                                                                                <  1:7439040/149‑1 (MQ=255)
   aaTCTTTCAAATCTAGTGTAGGTTCATAGATAAGAACCGTTATCCACTTATCTTGCAAGAGTTTAATCACAACAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCAGTAAATTCCAACAGATTTTGGAGAt                                                                                                                                              >  2:2198690/1‑149 (MQ=255)
       tttCAAATCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTac                                                                                                                                          >  2:6213086/1‑148 (MQ=255)
       tttCAAATCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAg                                                                                                                                          >  1:1948523/1‑149 (MQ=255)
          cAAATCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCa                                                                                                                                       >  2:8662154/1‑149 (MQ=255)
           aaaTCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCAt                                                                                                                                      <  1:5891561/149‑1 (MQ=255)
            aaTCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATa                                                                                                                                     <  1:5583934/149‑1 (MQ=255)
            aaTCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATa                                                                                                                                     <  1:8292039/149‑1 (MQ=255)
              tCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAAt                                                                                                                                   >  1:1783958/1‑149 (MQ=255)
              tCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAAt                                                                                                                                   <  1:2300819/149‑1 (MQ=255)
              tCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAAt                                                                                                                                   <  1:9378682/149‑1 (MQ=255)
                tAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCt                                                                                                                                 >  1:8389210/1‑149 (MQ=255)
                 aGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTg                                                                                                                                <  1:3152197/149‑1 (MQ=255)
                  gtgtAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTATCCATAATCTGc                                                                                                                               >  2:9375945/1‑149 (MQ=255)
                            aTAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAAtgt                                                                                                                     <  2:8816139/149‑1 (MQ=255)
                              aGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGc                                                                                                                   >  1:7729591/1‑149 (MQ=255)
                              aGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGc                                                                                                                   >  1:4183087/1‑149 (MQ=255)
                               gATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCt                                                                                                                  <  2:9794748/149‑1 (MQ=255)
                               gATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCt                                                                                                                  >  2:5131571/1‑149 (MQ=255)
                                aTAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTg                                                                                                                 >  2:5296927/1‑149 (MQ=255)
                                aTAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTg                                                                                                                 >  1:9735/1‑149 (MQ=255)
                                aTAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTg                                                                                                                 >  2:7165564/1‑149 (MQ=255)
                                aTAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTg                                                                                                                 <  1:804029/149‑1 (MQ=255)
                                 tAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGt                                                                                                                <  2:1005912/149‑1 (MQ=255)
                                  aaGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGtt                                                                                                               <  2:7515907/149‑1 (MQ=255)
                                    gAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGtttt                                                                                                             >  1:324/1‑149 (MQ=255)
                                    gAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGtttt                                                                                                             >  1:8723273/1‑149 (MQ=255)
                                      aCCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCt                                                                                                           <  2:8139418/149‑1 (MQ=255)
                                       ccGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTg                                                                                                          >  1:239582/1‑149 (MQ=255)
                                        cGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAGTAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTgg                                                                                                         <  2:7358957/149‑1 (MQ=255)
                                          ttATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGtt                                                                                                       <  2:10330846/149‑1 (MQ=255)
                                            aTCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTc                                                                                                     >  1:10254246/1‑149 (MQ=255)
                                            aTCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTc                                                                                                     >  2:7708923/1‑149 (MQ=255)
                                             tCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCg                                                                                                    >  1:6181936/1‑149 (MQ=255)
                                              ccccTTATCTCAGAGCAGTTTCATGACATCGCTATTCGAAGTCTGTGTTAAATCATATGACCCAGCTTTCCTGTCCTACCAGGAAGTTCCAACAGTTCTTGGAGATTTAGCCATAATCTGCGCAACAATGTGCTGTTTTCTGGTTTCGt                                                                                                   <  2:378902/149‑1 (MQ=255)
                                                ccTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGttt                                                                                                 <  2:5622701/149‑1 (MQ=255)
                                                    aTCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTgatt                                                                                             >  2:3470447/1‑146 (MQ=255)
                                                      cTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCTAACAGTTTTTGGAGATTTAGCCATAACCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTgattta                                                                                           >  2:1515524/1‑144 (MQ=255)
                                                        tGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTgatttgac                                                                                         <  2:7901125/149‑8 (MQ=255)
                                                          cAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACaa                                                                                       >  1:6824647/1‑149 (MQ=255)
                                                           aaGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAAt                                                                                      >  1:6518146/1‑149 (MQ=255)
                                                           aaGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAAt                                                                                      <  2:5926802/149‑1 (MQ=255)
                                                             gagTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTCTTTTCTGGTTTCGTTTGATTTGACAATAg                                                                                    <  2:6485269/149‑1 (MQ=255)
                                                              agTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGc                                                                                   >  2:1326917/1‑149 (MQ=255)
                                                                 ttCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGt                                                                                <  1:3397990/149‑1 (MQ=255)
                                                                   catcacatcaATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGtca                                                                              <  1:7609778/149‑1 (MQ=255)
                                                                     tcacatcaATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGtcatc                                                                            <  1:1094380/149‑1 (MQ=255)
                                                                      cacatcaATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGtcatca                                                                           <  1:856686/149‑1 (MQ=255)
                                                                       acatcaATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCaa                                                                          >  2:3257316/1‑149 (MQ=255)
                                                                        catcaATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAAc                                                                         >  2:4188926/1‑149 (MQ=255)
                                                                             ataatCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTg                                                                    >  1:1577163/1‑149 (MQ=255)
                                                                                 tCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGa                                                                >  2:3657010/1‑149 (MQ=255)
                                                                                  cGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGaa                                                               <  2:2551867/149‑1 (MQ=255)
                                                                                    aaCTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACg                                                             >  2:4520303/1‑149 (MQ=255)
                                                                                    aaCTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACg                                                             >  1:2972141/1‑149 (MQ=255)
                                                                                     aCTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGc                                                            >  1:3525921/1‑149 (MQ=255)
                                                                                     aCTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGc                                                            <  1:6045661/149‑1 (MQ=255)
                                                                                      ctctGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCt                                                                                                           <  1:3725109/101‑1 (MQ=255)
                                                                                      ctctGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGcc                                                           <  1:5168746/149‑1 (MQ=255)
                                                                                      ctctGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGcc                                                           >  2:2716437/1‑149 (MQ=255)
                                                                                       tctgtctgAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGccc                                                          >  2:3003189/1‑149 (MQ=255)
                                                                                        ctgtctgAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCt                                                         <  1:8941424/149‑1 (MQ=255)
                                                                                          gtctgAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCa                                                       >  1:4272071/1‑149 (MQ=255)
                                                                                          gtctgAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCa                                                       >  2:4789739/1‑149 (MQ=255)
                                                                                               aaaTTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACt                                                  <  2:6377232/149‑1 (MQ=255)
                                                                                                        gACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAg                                         <  1:4426757/149‑1 (MQ=255)
                                                                                                         aCCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAgc                                        <  2:7797681/149‑1 (MQ=255)
                                                                                                             aGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAgctgct                                    >  2:8356873/1‑149 (MQ=255)
                                                                                                              gCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGCGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTCGCCAAAAGCTGCtt                                   >  2:9478181/1‑149 (MQ=255)
                                                                                                               cTTTCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCttt                                  >  2:8033750/1‑149 (MQ=255)
                                                                                                                tttCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTg                                 >  1:782152/1‑149 (MQ=255)
                                                                                                                 ttCATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGa                                <  1:6949204/149‑1 (MQ=255)
                                                                                                                   cATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGt                              <  1:1214683/149‑1 (MQ=255)
                                                                                                                   cATGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGt                              >  1:1375095/1‑149 (MQ=255)
                                                                                                                    aTGACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTc                             <  1:7160115/149‑1 (MQ=255)
                                                                                                                      gACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCtt                           >  1:10019264/1‑149 (MQ=255)
                                                                                                                      gACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCtt                           <  1:2747495/149‑1 (MQ=255)
                                                                                                                      gACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCtt                           <  1:4877950/149‑1 (MQ=255)
                                                                                                                      gACCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCtt                           >  1:3810722/1‑149 (MQ=255)
                                                                                                                       aCCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCTATAATCTGCTCAACAATGTACTGTTTTCTGGTTTCGTTTGATTCTACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGTCCAACAGCTGCATTGAGTCttt                          >  2:8967594/1‑149 (MQ=255)
                                                                                                                       aCCAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCttt                          <  1:5509684/149‑1 (MQ=255)
                                                                                                                        ccAACCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATACTCTGCTCAACAATGTGCTGTTTTTTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGGTGCTTTGAGTCTTTc                         >  2:6917483/1‑149 (MQ=255)
                                                                                                                          aaCCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCgg                       >  1:4658944/1‑149 (MQ=255)
                                                                                                                          aaCCGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCgg                       <  1:2092575/149‑1 (MQ=255)
                                                                                                                            ccGGTAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCa                     <  1:2581554/149‑1 (MQ=255)
                                                                                                                                tAAATTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACa                 >  2:1812594/1‑149 (MQ=255)
                                                                                                                                 aaaTTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACACTGTGCGGTTGTCTGGTCTCGTTTGATTTGACAATAGCCGTCATCAACCCCTGAGGAACGCCCTCATAACTGGCCAACAGCTGCTTTGAGTCTTTCGGCAAACaa                <  1:665130/149‑1 (MQ=255)
                                                                                                                                 aaaTTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACaa                <  1:6341508/149‑1 (MQ=255)
                                                                                                                                  aaTTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAAt               >  1:5299706/1‑149 (MQ=255)
                                                                                                                                   aTTCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATa              >  2:6687117/1‑149 (MQ=255)
                                                                                                                                    ttCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATaa             >  1:9227924/1‑149 (MQ=255)
                                                                                                                                     tCCAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCaaa                   <  2:2390852/142‑1 (MQ=255)
                                                                                                                                      ccAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAacc           <  2:1167945/149‑1 (MQ=255)
                                                                                                                                       cAACAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAacca          >  2:5000143/1‑149 (MQ=255)
                                                                                                                                         aCAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAaccacc        <  2:2027325/149‑1 (MQ=255)
                                                                                                                                         aCAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAaccacc        >  1:994887/1‑149 (MQ=255)
                                                                                                                                          cAGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAAccacca       >  1:8153699/1‑149 (MQ=255)
                                                                                                                                           aGTTTTTGGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCAt      <  1:4018676/149‑1 (MQ=255)
                                                                                                                                             tttttgGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATaa    >  1:954323/1‑149 (MQ=255)
                                                                                                                                               tttgGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAAcc  <  2:9209617/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CATAATCTTTCAAATCTAGTGTAGGTTCATAGATAAGAACCGTTATCCCCTTATCTTGCAAGAGTTTCATCACATCAATAATCGAACTCTGTCTGAAATTATCTGACCCAGCTTTCATGACCAACCGGTAAATTCCAACAGTTTTTTGAGATTTAGCCATAATCTGCTCAACAATGTGCTGTTTTCTGGTTTCGTTTGTATTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACC  >  AM406671/1588047‑1588338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑