Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 1,588,356 T→A N243Y (AAT→TAT)  ugd ← UDP‑glucose 6‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM4066711,588,3560TA99.8% 3572.6 / NA 914N243Y (AAT→TAT) ugdUDP‑glucose 6‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base A (417/494);  total (418/496)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 959 total aligned reads displayed.

CTGCTCAACAATGTGCTGTTTTC‑TGGTTTCGTTTGTATTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATTATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTATTAGCAAAAAGTTTGATTGCTTCT  >  AM406671/1588210‑1588501
                                                                                                                                                   |                                                                                                                                                 
  ccTCAAC‑ATGTGCCTTTTTCTTGGTTTCGTTGGT‑TTTACAAAAGGCCTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAAtgtg                                                                                                                                              <  1:3345745/148‑1 (MQ=255)
    tCAACAATGTGCTGTTTTC‑TGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATgtgt                                                                                                                                             >  2:9151972/1‑149 (MQ=255)
     caacacTGCCCTGTTTTC‑TGGTTTCGTTTGCTTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCTTAACCAAGACTTGGATTATTATAATGTGTc                                                                                                                                            <  2:6366831/149‑1 (MQ=255)
     caacaaTGTGCTGTTTTC‑TGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTc                                                                                                                                            >  2:5265952/1‑149 (MQ=255)
      aacaaTGTGCTGTTTTC‑TGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTcc                                                                                                                                           >  2:39281/1‑149 (MQ=255)
       acaaTGTGCTGTTTTC‑TGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTccc                                                                                                                                          <  2:279146/149‑1 (MQ=255)
           tgtgCTGTTTTC‑TGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATc                                                                                                                                      >  1:1549418/1‑149 (MQ=255)
           tgtgCTGTTTTC‑TGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATc                                                                                                                                      <  1:4036394/149‑1 (MQ=255)
           tgtgCTGTTTTC‑TGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATc                                                                                                                                      >  2:4358446/1‑149 (MQ=255)
             tgCTGTTTTC‑TGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCg                                                                                                                                    >  1:7337732/1‑149 (MQ=255)
               cTGTTTTC‑TGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTg                                                                                                                                  <  2:3095822/149‑1 (MQ=255)
                tGTTTTC‑TGGTTTCGTTTGGTTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCCCCATAACCAAAACTTGGATTATTATAATGTGTCCCTTTCCGTGa                                                                                                                                 <  2:9544213/149‑1 (MQ=255)
                tGTTTTC‑TGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGa                                                                                                                                 <  2:4529630/149‑1 (MQ=255)
                   tttC‑TGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATc                                                                                                                              <  2:1577163/149‑1 (MQ=255)
                    ttC‑TGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATcc                                                                                                                             >  2:9432420/1‑149 (MQ=255)
                    ttC‑TGGTTTCGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTAATATAATGTGTCCCAATCCGTGAATcc                                                                                                                             >  1:6707951/1‑149 (MQ=255)
                              cGTTTGATTTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAaca                                                                                                                    <  2:9697275/149‑1 (MQ=255)
                               gTTTGATTTGACAATAGCCGTCATCATCTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAACCTTGGATTATTATAATGTGTCCCAATCCGTGAATCCATCCCAacac                                                                                                                   <  1:8949992/149‑1 (MQ=255)
                                tttgatttGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACAcc                                                                                                                  >  1:3620119/7‑149 (MQ=255)
                                 ttgatttGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCg                                                                                                                 >  1:792519/6‑149 (MQ=255)
                                 ttgatttGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCg                                                                                                                 >  1:6872996/6‑149 (MQ=255)
                                 ttgatttGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCg                                                                                                                 >  1:8723425/6‑149 (MQ=255)
                                 ttgatttGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCg                                                                                                                 >  2:9829489/6‑149 (MQ=255)
                                  tgatttGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGt                                                                                                                <  2:2361072/145‑1 (MQ=255)
                                    atttGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTta                                                                                                              <  1:5894877/147‑1 (MQ=255)
                                     tttGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTtaa                                                                                                             >  2:5668563/2‑149 (MQ=255)
                                      ttGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAACCTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTtaat                                                                                                            >  2:7786500/1‑149 (MQ=255)
                                      ttGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTtaat                                                                                                            >  1:1775958/1‑149 (MQ=255)
                                      ttGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTtaat                                                                                                            <  1:239782/149‑1 (MQ=255)
                                      ttGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTtaat                                                                                                            <  2:2173645/149‑1 (MQ=255)
                                      ttGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTtaat                                                                                                            <  1:2601162/149‑1 (MQ=255)
                                       tGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTtaata                                                                                                           <  2:5044702/149‑1 (MQ=255)
                                             tAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGt                                                                                                     >  1:6974628/1‑149 (MQ=255)
                                               gCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTg                                                                                                   >  1:3897961/1‑149 (MQ=255)
                                               gCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTg                                                                                                   <  2:7272938/149‑1 (MQ=255)
                                                  gTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACt                                                                                                <  2:8838698/149‑1 (MQ=255)
                                                   tcatcaACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTa                                                                                               >  1:1382802/1‑149 (MQ=255)
                                                    catcaACTCCTGAGTAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGCCTAt                                                                                              >  1:3777927/1‑149 (MQ=255)
                                                    catcaACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTAt                                                                                              <  1:5040041/149‑1 (MQ=255)
                                                     atcaACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTAtt                                                                                             >  2:6588551/1‑149 (MQ=255)
                                                      tcaACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTAttt                                                                                            <  1:5253993/149‑1 (MQ=255)
                                                      tcaACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTAttt                                                                                            <  1:3124741/149‑1 (MQ=255)
                                                      tcaACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTAttt                                                                                            >  2:9068694/1‑149 (MQ=255)
                                                        aaCTCCTGAGGAACGCCCTCATAACTGTCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTaa                                                                                          >  2:352420/1‑149 (MQ=255)
                                                        aaCTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTaa                                                                                          <  1:9152069/149‑1 (MQ=255)
                                                          cTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAAtt                                                                                        <  1:6537520/149‑1 (MQ=255)
                                                            ccTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGa                                                                                      <  2:19469/149‑1 (MQ=255)
                                                             cTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGATTCTTTCGGCAAACAATAACCACCATAACCAAACCTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGAt                                                                                     <  2:6262309/149‑1 (MQ=255)
                                                             cTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGAt                                                                                     <  1:2081040/149‑1 (MQ=255)
                                                             cTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGAt                                                                                     <  2:8630704/149‑1 (MQ=255)
                                                              tGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGAtt                                                                                    >  1:852592/1‑149 (MQ=255)
                                                                aGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGAtttt                                                                                  <  2:8292180/149‑1 (MQ=255)
                                                                aGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGAtttt                                                                                  <  2:3744499/149‑1 (MQ=255)
                                                                       cccTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGc                                                                           <  2:173337/149‑1 (MQ=255)
                                                                           cATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATaa                                                                       <  2:4183087/149‑1 (MQ=255)
                                                                           cATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATaa                                                                       >  2:7479990/1‑149 (MQ=255)
                                                                            aTAACTGGCCAAATGCTGCTTTGAGTCTTTCGGCAAACAAGAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAg                                                                      <  2:5905448/149‑1 (MQ=255)
                                                                              aaCTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTa                                                                    >  2:7127962/1‑149 (MQ=255)
                                                                                 tGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCa                                                                 <  1:285120/149‑1 (MQ=255)
                                                                                   gCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAg                                                               <  1:10371411/149‑1 (MQ=255)
                                                                                        aaGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAGTCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCAt                                                          <  1:6056578/149‑1 (MQ=255)
                                                                                        aaGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCAt                                                          <  1:7722280/149‑1 (MQ=255)
                                                                                         aGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCAtt                                                         >  1:1099825/1‑149 (MQ=255)
                                                                                           ctgctTTGAGTCTTTCGGCAAACAATACCCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCACCCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTaa                                                       <  1:10009104/149‑1 (MQ=255)
                                                                                             gctTTGAGTCTTTCGGCAAACAATACCCACCATATCCAAACCTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTACTTGATTTTTTTCGGCATAAGTATCAAGTTCATTaaag                                                     <  1:2184389/149‑1 (MQ=255)
                                                                                             gctTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTaaag                                                     >  1:46590/1‑149 (MQ=255)
                                                                                               tttGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTaaagaa                                                   <  1:6213086/149‑1 (MQ=255)
                                                                                                 tGAGTCTTTCGGCAAACAATAACCCCCATAACCATAACTTGGATTATTATAATGTGTCCCAATCCGTTAATTCTACCCAACCCCGTTAATAACATGTTGACCATTTAATTGATTTTTTTCCGCATAAGTATCAACTTCATTaatgaaag                                                 <  2:681566/149‑1 (MQ=255)
                                                                                                 tGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTaaagaaag                                                 <  1:8356873/149‑1 (MQ=255)
                                                                                                  gAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGc                                                >  1:10207450/1‑149 (MQ=255)
                                                                                                      cTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCAcg                                            <  2:10074070/149‑1 (MQ=255)
                                                                                                        ttCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCAcgcg                                          >  1:4358978/1‑149 (MQ=255)
                                                                                                        ttCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCAcgcg                                          <  1:7143806/149‑1 (MQ=255)
                                                                                                          cGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCa                                        <  2:324/149‑1 (MQ=255)
                                                                                                          cGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCa                                        <  2:1256392/149‑1 (MQ=255)
                                                                                                                aCAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCaa                                  <  1:1263083/149‑1 (MQ=255)
                                                                                                                aCAATAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCaa                                  >  2:7646670/1‑149 (MQ=255)
                                                                                                                  aaTAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAagg                                >  1:2967907/1‑149 (MQ=255)
                                                                                                                    tAACCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAaggta                              <  2:4779634/149‑1 (MQ=255)
                                                                                                                     aaCCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAaggtag                             <  1:8165249/149‑1 (MQ=255)
                                                                                                                     aaCCACCATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAaggtag                             <  2:792519/149‑1 (MQ=255)
                                                                                                                      accaccATAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAaggtagg                            <  1:9344395/149‑1 (MQ=255)
                                                                                                                       ccaccaTAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGTCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAaggtaggt                           >  2:5531472/1‑149 (MQ=255)
                                                                                                                        caccaTAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCTTTAAAGAAATCCACGCGCATCGCAAggtaggta                          >  2:8410363/1‑149 (MQ=255)
                                                                                                                        caccaTAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAggtaggta                          <  1:9617583/149‑1 (MQ=255)
                                                                                                                        caccaTAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAggtaggta                          >  1:1854255/1‑149 (MQ=255)
                                                                                                                          ccaTAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTAtt                        <  1:3463155/149‑1 (MQ=255)
                                                                                                                             tAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTATTAGc                     >  2:10465454/1‑149 (MQ=255)
                                                                                                                             tAACCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTATTAGc                     >  2:822521/1‑149 (MQ=255)
                                                                                                                              aaCCAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGTCACGCGCATCGCAAGGTAGGTATTAGCa                    >  1:7580328/1‑149 (MQ=255)
                                                                                                                                 cAAAACTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTATTAGCaaaa                 <  1:2716437/149‑1 (MQ=255)
                                                                                                                                  aaaaCTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTATTAGCaaaaa                >  1:9834698/1‑149 (MQ=255)
                                                                                                                                   aaaCTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACAGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTATTAGCAAAAAg               >  2:3411594/1‑149 (MQ=255)
                                                                                                                                    aaCTTGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTATTAGCAAAAAGt              <  1:5697892/149‑1 (MQ=255)
                                                                                                                                        tGGATTATTATAATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTATTAGCAAAAAGTTTGa          <  1:7419738/149‑1 (MQ=255)
                                                                                                                                           attattataATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTATTAGCAAAAAGTTTGATTg       >  1:5480/1‑149 (MQ=255)
                                                                                                                                              attataatGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTATTAGCAAAAAGTTTGATTGCtt    >  1:4828280/1‑149 (MQ=255)
                                                                                                                                              attataatGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTATTAGCAAAAAGTTTGATTGCtt    <  1:6399767/149‑1 (MQ=255)
                                                                                                                                              attataatGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTATTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTATTAGCAAAAAGTTTGATTGCtt    >  2:8127419/1‑149 (MQ=255)
                                                                                                                                               ttataatGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTATTAGCAAAAAGTTTGATTGCTTc   <  2:255525/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CTGCTCAACAATGTGCTGTTTTC‑TGGTTTCGTTTGTATTGACAATAGCCGTCATCAACTCCTGAGGAACGCCCTCATAACTGGCCAAAAGCTGCTTTGAGTCTTTCGGCAAACAATAACCACCATAACCAAAACTTGGATTATTATTATGTGTCCCAATCCGTGAATCCAACCCAACACCGTTAATAATATGTTGACTATTTAATTGATTTTTTTCGGCATAAGTATCAAGTTCATTAAAGAAAGCCACGCGCATCGCAAGGTAGGTATTAGCAAAAAGTTTGATTGCTTCT  >  AM406671/1588210‑1588501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑