Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 1,610,761 T→C I430V (ATT→GTT)  llmg_1634 ← putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM4066711,610,7610TC99.9% 4397.5 / NA 1064I430V (ATT→GTT) llmg_1634putative membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (508/554);  minor base G (0/1);  total (508/556)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1085 total aligned reads displayed.

TCCCAAAAGCGTAGCAAAGAAAATGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAATATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTGAGATAGAAATTCAACTTCATGGGT  >  AM406671/1610615‑1610906
                                                                                                                                                  |                                                                                                                                                 
 cccAAAAGCGTAGCAAAGAAAATGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACAtt                                                                                                                                                >  1:3111817/1‑149 (MQ=255)
  ccAAAAGCGTAGCAAAGAAAATGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTc                                                                                                                                               <  2:7672243/149‑1 (MQ=255)
      aaGCGTAGCAAAGACAATGATAAAAACCCGAAATCCCCCAGAACCCCTTGTAAAAAGTTGAGCCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATCATGAAGTGAAATCAGATATACCCCAATAACATTCCCaa                                                                                                                                           >  2:1300119/1‑149 (MQ=255)
      aaGCGTAGCAAAGAAAATGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCaa                                                                                                                                           >  2:3650262/1‑149 (MQ=255)
       aGCGTAGCAAAGAAAATGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAAt                                                                                                                                          <  2:3524038/149‑1 (MQ=255)
        gCGTAGCAAAGAAAATGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAAtt                                                                                                                                         >  1:7948630/1‑149 (MQ=255)
        gCGTAGCAAAGAAAATGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAAtt                                                                                                                                         >  1:203745/1‑149 (MQ=255)
           tAGCAAAGAAAATGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAAc                                                                                                                                      <  1:2291212/149‑1 (MQ=255)
            aGCAAAGAAAATGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACACCAATAACATTCCCAATTAACa                                                                                                                                     >  2:6830894/1‑149 (MQ=255)
             gCAAAGAAAATGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAg                                                                                                                                    <  2:10300185/149‑1 (MQ=255)
                 aGAAAATGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCa                                                                                                                                >  2:7492933/1‑149 (MQ=255)
                 aGAAAATGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCa                                                                                                                                >  2:6611939/1‑149 (MQ=255)
                   aaaaTGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCaaa                                                                                                                              <  1:5680057/149‑1 (MQ=255)
                    aaaTGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAag                                                                                                                             <  1:4609474/149‑1 (MQ=255)
                     aaTGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAaga                                                                                                                            <  2:3053896/149‑1 (MQ=255)
                     aaTGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAaga                                                                                                                            >  2:7957151/1‑149 (MQ=255)
                         aTAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCaa                                                                                                                        <  2:8132886/149‑1 (MQ=255)
                          tAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAg                                                                                                                       <  1:7080162/149‑1 (MQ=255)
                             aaaCCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTa                                                                                                                    >  1:9137693/1‑149 (MQ=255)
                             aaaCCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTa                                                                                                                    <  1:10199558/149‑1 (MQ=255)
                              aaCCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTaa                                                                                                                   >  2:10083946/1‑149 (MQ=255)
                               aCCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAAc                                                                                                                  <  1:5461986/149‑1 (MQ=255)
                               aCCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAAc                                                                                                                  >  2:4156423/1‑149 (MQ=255)
                               aCCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAAc                                                                                                                  <  2:1544013/149‑1 (MQ=255)
                                 ccGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAg                                                                                                                >  1:7435887/1‑149 (MQ=255)
                                  cGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAgg                                                                                                               <  1:9423651/149‑1 (MQ=255)
                                   gAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAggg                                                                                                              >  1:6118420/1‑149 (MQ=255)
                                         ccccAGAACCTCTTGTAAATAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTc                                                                                                        <  2:5112057/149‑1 (MQ=255)
                                         ccccAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTc                                                                                                        >  1:8502715/1‑149 (MQ=255)
                                          cccAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCa                                                                                                       >  2:2380375/1‑149 (MQ=255)
                                            cAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGt                                                                                                     >  1:10007139/1‑149 (MQ=255)
                                             aGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCCGTa                                                                                                    >  2:5312890/1‑149 (MQ=255)
                                             aGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTa                                                                                                    >  2:2882035/1‑149 (MQ=255)
                                             aGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTa                                                                                                    <  2:2139907/149‑1 (MQ=255)
                                              gAACCTCGTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATGTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGAATTTCAGTaa                                                                                                   >  2:5561253/1‑149 (MQ=255)
                                                aCCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTaaaa                                                                                                 <  2:3245852/149‑1 (MQ=255)
                                                aCCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTaaaa                                                                                                 <  2:6128980/149‑1 (MQ=255)
                                                 ccTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAAt                                                                                                <  2:1964680/149‑1 (MQ=255)
                                                   tctTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATAc                                                                                              >  1:3490449/1‑149 (MQ=255)
                                                    ctTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTaa                                                                                                   >  2:4038170/1‑143 (MQ=255)
                                                      tGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCaa                                                                                           <  2:7253692/149‑1 (MQ=255)
                                                      tGTAAAAAGATGAGTCAACGCAGACCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAAACAGATATACCACAATAACATTCACAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCaa                                                                                           >  2:3829906/1‑149 (MQ=255)
                                                          aaaaGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCAc                                                                                       >  1:7731976/1‑149 (MQ=255)
                                                            aaGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTa                                                                                     <  1:9601506/149‑1 (MQ=255)
                                                              gTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTaaa                                                                                   <  2:8976958/149‑1 (MQ=255)
                                                                   gTCAACGCAGTCCAATTTAAATTTAACAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCGAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAaagataa                                                                              <  1:6718153/149‑1 (MQ=255)
                                                                      aaCGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGGTATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAaagataagat                                                                           <  2:10445661/149‑1 (MQ=255)
                                                                        cGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTa                                                                         <  1:2920857/149‑1 (MQ=255)
                                                                        cGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTa                                                                         >  2:9199773/1‑149 (MQ=255)
                                                                         gCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTaa                                                                        >  2:3415976/1‑149 (MQ=255)
                                                                          cAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTCAAATACCAATCACTAAAGATAAGATTaaa                                                                       <  2:6467016/149‑1 (MQ=255)
                                                                          cAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTaaa                                                                       >  1:754454/1‑149 (MQ=255)
                                                                           aGTCCAATTTAAATTTAAGAGATGCCAATCCCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAAATAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAAt                                                                      <  2:5807779/149‑1 (MQ=255)
                                                                            gTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATc                                                                     <  2:8516497/149‑1 (MQ=255)
                                                                             tCCAATTTAAATTAAAGAGCTGCCAATCTTTGACAAAAAAACGAAGTAAAATCAGATATACTTCAATAACATTCCCAAATAACAGCACAAAGAGAAATGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCa                                                                    >  2:9593132/1‑149 (MQ=255)
                                                                               cAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAg                                                                  <  1:6911966/149‑1 (MQ=255)
                                                                                aatttaaatttaAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGa                                                                 >  1:2763686/1‑149 (MQ=255)
                                                                                        tttaaGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTg                                                         <  2:4629401/149‑1 (MQ=255)
                                                                                         ttaaGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGa                                                        >  1:6494517/1‑149 (MQ=255)
                                                                                         ttaaGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAACTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATACGATTAAATCAAGAATTAGCTGa                                                        >  2:833108/1‑149 (MQ=255)
                                                                                          taaGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGaa                                                       <  1:8975100/149‑1 (MQ=255)
                                                                                            agagATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCACAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATa                                                     >  1:6343433/1‑149 (MQ=255)
                                                                                             gagaTGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATaa                                                    <  1:9817538/149‑1 (MQ=255)
                                                                                             gagaTGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATaa                                                    <  1:1901045/149‑1 (MQ=255)
                                                                                              agaTGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCAAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATaaa                                                   >  2:4337438/1‑149 (MQ=255)
                                                                                               gaTGCCAATCTCTGACAAAATAATGAAGTAAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATaaaa                                                  <  1:8361986/149‑1 (MQ=255)
                                                                                                aTGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATaaaaa                                                 <  1:3315548/149‑1 (MQ=255)
                                                                                                aTGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATaaaaa                                                 >  1:4407048/1‑149 (MQ=255)
                                                                                                 tGCCAATCTCTGACAAAATAATGAAGTTAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATaaaaaa                                                <  1:420463/149‑1 (MQ=255)
                                                                                                  gCCAATCTCTGACAAAATGATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATACAAAAg                                               >  1:1355984/1‑149 (MQ=255)
                                                                                                      aTCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCaa                                           >  1:8680366/1‑149 (MQ=255)
                                                                                                      aTCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCaa                                           >  1:5137589/1‑149 (MQ=255)
                                                                                                      aTCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCaa                                           <  2:9392573/149‑1 (MQ=255)
                                                                                                       tctcTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCaaa                                          >  2:4850128/1‑149 (MQ=255)
                                                                                                        cactaaCAAAATAATGAAAAGAAATGAGATATACCCCAATAACATTACCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAAACAAGAACTAGTTGAATAAAAAAGGCaaaa                                         <  2:245575/144‑1 (MQ=255)
                                                                                                         tctGACAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAAt                                        <  2:5983552/149‑1 (MQ=255)
                                                                                                             aCAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGaa                                                                <  1:169162/121‑1 (MQ=255)
                                                                                                             aCAAAATAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGaa                                                                >  2:169162/1‑121 (MQ=255)
                                                                                                               aaaaTAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAAtt                                  <  1:1147801/149‑1 (MQ=255)
                                                                                                               aaaaTAATGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAAtt                                  >  1:9971623/1‑149 (MQ=255)
                                                                                                                  ataatGAAGTGAAATCAAACATACCCCAAAAACATTCCCGATTAACAACCCAAAGAACAAGGTAACCGGGACTTACAGCAAAATACCAATCACCAAAGATAACATTAAATCAAGAACCAGTTGAATAAAAAAGGCAAAATGAAATTTCa                               <  1:2575282/149‑1 (MQ=255)
                                                                                                                    aatGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCAct                             <  2:2762937/149‑1 (MQ=255)
                                                                                                                     atGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCActc                            <  2:9839566/149‑1 (MQ=255)
                                                                                                                     atGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCActc                            >  1:3751147/1‑149 (MQ=255)
                                                                                                                     atGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCActc                            >  1:1525646/1‑149 (MQ=255)
                                                                                                                      tGAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCActct                           >  1:4748106/1‑149 (MQ=255)
                                                                                                                       gAAGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTg                          <  2:9082043/149‑1 (MQ=255)
                                                                                                                        aaGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTga                         >  2:1812483/1‑149 (MQ=255)
                                                                                                                         aGTGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTgag                        >  1:8162248/1‑149 (MQ=255)
                                                                                                                           tGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAGAAGGCAAAATGAAATTTCACTCTGAGAt                      >  1:7187482/1‑149 (MQ=255)
                                                                                                                           tGAAATCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTGAGAt                      >  1:10326623/1‑149 (MQ=255)
                                                                                                                              aaTCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTAACTCTGAGATAGa                   >  2:2978285/1‑149 (MQ=255)
                                                                                                                               aTCAGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTGAGATAGaa                  >  2:8828316/1‑149 (MQ=255)
                                                                                                                                  aGATATACCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAAATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTGAGATAGAAAtt               <  2:2557388/149‑1 (MQ=255)
                                                                                                                                      ataCCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTGAGATAGAAATTCAAc           >  1:6579259/1‑149 (MQ=255)
                                                                                                                                       taCCCCAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTGAGATAGAAATTCAACt          <  1:7305872/149‑1 (MQ=255)
                                                                                                                                            cAATAACATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTGAGATAGAAATTCAACTTCATg     >  2:707810/1‑149 (MQ=255)
                                                                                                                                             aataacATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTGAGATAGAAATTCAACTTCATgg    <  1:4023372/149‑1 (MQ=255)
                                                                                                                                               taacaTTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTGAGATAGAAATTCAACTTCATGGGt  <  2:6330973/149‑1 (MQ=255)
                                                                                                                                               taacaTTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTGAGATAGAAATTCAACTTCATGGGt  <  1:5883416/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TCCCAAAAGCGTAGCAAAGAAAATGATAAAAACCCGAAATCCCCCAGAACCTCTTGTAAAAAGTTGAGTCAACGCAGTCCAATTTAAATTTAAGAGATGCCAATCTCTGACAAAATAATGAAGTGAAATCAGATATACCCCAATAATATTCCCAATTAACAGCCCAAAGAGCAAGGTAACAGGGACTTTCAGTAAAATACCAATCACTAAAGATAAGATTAAATCAAGAACTAGTTGAATAAAAAAGGCAAAATGAAATTTCACTCTGAGATAGAAATTCAACTTCATGGGT  >  AM406671/1610615‑1610906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑