Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 447,854 G→A A98T (GCT→ACT)  llmg_0454 → putative beta‑glucosides‑specific IIBC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM406671447,8540GA99.7% 5349.0 / NA 1383A98T (GCT→ACT) llmg_0454putative beta‑glucosides‑specific IIBC component
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base A (644/733);  minor base C (1/3);  total (647/736)
Fisher's exact test for biased strand distribution p-value = 6.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1394 total aligned reads displayed.

TACCTGGTGTCGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTGCTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGATTATTAAGTATCTT  >  AM406671/447708‑447999
                                                                                                                                                  |                                                                                                                                                 
 aCCTGGTGTCGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTtactg                                                                                                                                                <  1:9985133/149‑1 (MQ=255)
  ccTGGTGTCGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTtactgc                                                                                                                                               >  2:3668473/1‑149 (MQ=255)
   cTGGTGTCGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCa                                                                                                                                              <  2:8067855/149‑1 (MQ=255)
    tGGTGTCGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCaa                                                                                                                                             <  1:1847455/149‑1 (MQ=255)
     ggTGTCGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAAc                                                                                                                                            >  2:5458308/1‑149 (MQ=255)
       tgtCGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAg                                                                                                                                          <  1:7996505/149‑1 (MQ=255)
        gtCGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGc                                                                                                                                         >  2:1128892/1‑149 (MQ=255)
         tCGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCa                                                                                                                                        <  1:9658665/149‑1 (MQ=255)
          cGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCAc                                                                                                                                       >  1:7329121/1‑149 (MQ=255)
          cGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGCTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCAc                                                                                                                                       >  1:1980352/1‑149 (MQ=255)
             tGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCgg                                                                                                                                    >  1:666371/1‑149 (MQ=255)
              ggAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGc                                                                                                                                   >  1:3809186/1‑149 (MQ=255)
               gAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAGGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCt                                                                                                                                  <  1:9772285/149‑1 (MQ=255)
               gAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCt                                                                                                                                  >  1:8822301/1‑149 (MQ=255)
               gAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCt                                                                                                                                  <  2:8534422/149‑1 (MQ=255)
               gAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCt                                                                                                                                  >  2:1325974/1‑149 (MQ=255)
                aGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTa                                                                                                                                 >  1:4258509/1‑149 (MQ=255)
                aGCTGTTTATAACTCAAACCTGAATCAATATCAAGTAGTTATTGGACAAGCCGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTa                                                                                                                                 <  1:5093487/149‑1 (MQ=255)
                 gCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACCAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAg                                                                                                                                <  1:1751499/149‑1 (MQ=255)
                 gCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAg                                                                                                                                <  1:862955/149‑1 (MQ=255)
                 gCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAg                                                                                                                                >  1:10178159/1‑149 (MQ=255)
                    gTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTgg                                                                                                                             >  2:2645836/1‑149 (MQ=255)
                      ttATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTa                                                                                                                           >  1:2302159/1‑149 (MQ=255)
                      ttATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTa                                                                                                                           >  2:6251045/1‑149 (MQ=255)
                               aaaCTTGAATCAATATCAAGTAGTTATTGGACATGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCTGTTGTTGATGAATATGCAACGGCGCAGGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACaaa                                                                                                                  >  2:1832248/1‑149 (MQ=255)
                                aaCTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACaaaa                                                                                                                 <  1:531338/149‑1 (MQ=255)
                                  cTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATc                                                                                                               <  2:337915/149‑1 (MQ=255)
                                     gAATCAATATCACGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCaa                                                                                                            <  1:4660425/149‑1 (MQ=255)
                                      aaTCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAAt                                                                                                           <  1:2437765/149‑1 (MQ=255)
                                       aTCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAAtt                                                                                                          >  2:945339/1‑149 (MQ=255)
                                        tCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTAGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCAACGGCTAGTGGTAAAAAACAAAATCCAATTg                                                                                                         >  2:8357187/1‑149 (MQ=255)
                                         cAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGt                                                                                                        >  1:6966713/1‑149 (MQ=255)
                                          aaTATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGtt                                                                                                       <  1:7644305/149‑1 (MQ=255)
                                           atatCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTc                                                                                                      >  2:2519234/1‑149 (MQ=255)
                                           atatCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTc                                                                                                      <  1:3978915/149‑1 (MQ=255)
                                               cAAGTAGTTATTGGACAAGCTGTAGACGATGTTTATGACGAGGTTGTTGAACAGCTGGGAGATGCAGTTGTTGATGAAGATGAAACGGCGCCAGCACTTGCTGCAACAGCACCGGCTCGTGGTAAAAAACAAAATCCAATTGTTCATGc                                                                                                  >  2:3158442/1‑149 (MQ=255)
                                                 agtagtTATTGGACAAGCTGTAGAAGATGTTTATTACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCtt                                                                                                <  2:640705/149‑1 (MQ=255)
                                                 agtagtTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCtt                                                                                                >  2:2365087/1‑149 (MQ=255)
                                                  gtagtTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCttt                                                                                               >  2:4388597/1‑149 (MQ=255)
                                                    agtTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTcc                                                                                             <  2:4957518/149‑1 (MQ=255)
                                                       tATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAg                                                                                          <  1:6152957/149‑1 (MQ=255)
                                                       tATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAg                                                                                          >  2:4215726/1‑149 (MQ=255)
                                                       tATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTAGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAg                                                                                          <  2:7643340/149‑1 (MQ=255)
                                                          tGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTgg                                                                                       <  2:9799218/149‑1 (MQ=255)
                                                             aCAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTa                                                                                    <  1:9533728/149‑1 (MQ=255)
                                                                 gCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCGACGTCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAGAATCCAATTGTTCATGCTTTCCAAGTGGTTATTgg                                                                                >  2:6620207/1‑149 (MQ=255)
                                                                  cTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTggg                                                                               <  1:8194184/149‑1 (MQ=255)
                                                                     tAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACa                                                                            >  2:5836752/1‑149 (MQ=255)
                                                                     tAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACa                                                                            <  1:8402909/149‑1 (MQ=255)
                                                                        aagaTGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAAGAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAAtt                                                                         >  2:7032049/1‑149 (MQ=255)
                                                                         agaTGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTa                                                                        <  2:6802605/149‑1 (MQ=255)
                                                                            tGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAg                                                                     >  1:4535905/1‑149 (MQ=255)
                                                                              tttATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGt                                                                   <  1:3665579/149‑1 (MQ=255)
                                                                                 aTGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCg                                                                <  1:3555163/149‑1 (MQ=255)
                                                                                        ggTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTCGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTc                                                         <  1:5394077/149‑1 (MQ=255)
                                                                                         gttgttGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTcc                                                        >  1:2594111/1‑149 (MQ=255)
                                                                                         gttgttGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTcc                                                        <  1:2126363/149‑1 (MQ=255)
                                                                                           tgttgAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCaa                                                      >  1:900809/1‑149 (MQ=255)
                                                                                           tgttgAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCaa                                                      >  1:7886407/1‑149 (MQ=255)
                                                                                           tgttgAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCaa                                                      <  2:5143175/149‑1 (MQ=255)
                                                                                             ttgAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAatt                                                    >  2:1573254/1‑149 (MQ=255)
                                                                                             ttgAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAatt                                                    <  1:3337342/149‑1 (MQ=255)
                                                                                              tgAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAatta                                                   <  1:5892901/149‑1 (MQ=255)
                                                                                                aaCAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAattatt                                                 >  1:1502168/1‑149 (MQ=255)
                                                                                                aaCAGCTTGGAGAGTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAattatt                                                 <  2:5633796/149‑1 (MQ=255)
                                                                                                 aCAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTg                                                >  2:3962485/1‑149 (MQ=255)
                                                                                                  cAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTgg                                               <  2:9045181/149‑1 (MQ=255)
                                                                                                    gCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGtt                                             <  2:10150674/149‑1 (MQ=255)
                                                                                                    gCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGtt                                             >  2:7434482/1‑149 (MQ=255)
                                                                                                    gCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGtt                                             <  2:7819848/149‑1 (MQ=255)
                                                                                                     cTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGttt                                            >  1:1351585/1‑149 (MQ=255)
                                                                                                      gtgtagaTTCAGTTGTTGATGAATATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTAATAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTa                                           <  2:1443721/145‑1 (MQ=255)
                                                                                                         gagaTTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACtt                                        >  2:2213428/1‑149 (MQ=255)
                                                                                                         gagaTTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACtt                                        >  1:7495662/1‑149 (MQ=255)
                                                                                                         gagaTTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACtt                                        <  1:482100/149‑1 (MQ=255)
                                                                                                           gaTTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGc                                      >  2:2881235/1‑149 (MQ=255)
                                                                                                            aTTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCg                                     >  2:5262584/1‑149 (MQ=255)
                                                                                                             ttCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCgg                                    <  2:2009811/149‑1 (MQ=255)
                                                                                                               cAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCt                                  >  2:7249739/1‑149 (MQ=255)
                                                                                                               cAGTTGTTGATGAAGATGCAACGGCGCAAGCACTGACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAGTCCAATGGTTCATGCTTTCCAAGTGGCTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCt                                  <  1:1162534/149‑1 (MQ=255)
                                                                                                                aGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTAGAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCtg                                 >  1:6430891/1‑149 (MQ=255)
                                                                                                                aGTTGTTGATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCtg                                 >  2:4696410/1‑149 (MQ=255)
                                                                                                                  ttgttgATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCtggt                               <  2:4530183/149‑1 (MQ=255)
                                                                                                                  ttgttgATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCtggt                               >  2:9597364/1‑149 (MQ=255)
                                                                                                                   tgttgATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCtggtg                              >  1:3093550/1‑149 (MQ=255)
                                                                                                                     ttgATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTggg                            >  1:1001336/1‑149 (MQ=255)
                                                                                                                     ttgATGAAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTggg                            <  1:9381540/149‑1 (MQ=255)
                                                                                                                         tgaAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGgatga                        >  1:5010119/1‑149 (MQ=255)
                                                                                                                          gaAGATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGgatgat                       >  1:4412021/1‑149 (MQ=255)
                                                                                                                             gATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTaa                    >  1:10318954/1‑149 (MQ=255)
                                                                                                                             gATGCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTaa                    <  1:6804798/149‑1 (MQ=255)
                                                                                                                               tGCAACGGCGCAAGCACTTACTGCAACAGCACCGGATAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATg                  >  2:9313588/1‑149 (MQ=255)
                                                                                                                                gCAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATgg                 <  1:2775365/149‑1 (MQ=255)
                                                                                                                                 cAACGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGa                <  1:9187830/149‑1 (MQ=255)
                                                                                                                                   aCGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGatt              <  2:6047612/149‑1 (MQ=255)
                                                                                                                                    cGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGatta             <  1:7165215/149‑1 (MQ=255)
                                                                                                                                    cGGCGCAAGCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGatta             <  2:10336945/149‑1 (MQ=255)
                                                                                                                                            gCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGATTATTAAGTAt     >  2:8746965/1‑149 (MQ=255)
                                                                                                                                            gCACTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGATTATTAAGTAt     <  1:5660245/149‑1 (MQ=255)
                                                                                                                                              aCTTACTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGATTATTAAGTATCt   <  1:7747929/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TACCTGGTGTCGCTGGAGCTGTTTATAACTCAAACTTGAATCAATATCAAGTAGTTATTGGACAAGCTGTAGAAGATGTTTATGACGAGGTTGTTGAACAGCTTGGAGATTCAGTTGTTGATGAAGATGCAACGGCGCAAGCACTTGCTGCAACAGCACCGGCTAGTGGTAAAAAACAAAATCCAATTGTTCATGCTTTCCAAGTGGTTATTGGGACAATTACAGGTTCGATGATTCCAATTATTGGTTTACTTGCGGCTGGTGGGATGATTAATGGATTATTAAGTATCTT  >  AM406671/447708‑447999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑