BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	1,681,037	T→C	E332G (GAA→GGA)	pepV ←	Dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	1,681,037	0	T	C	99.6%	4311.6 / NA	1044	E332G (GAA→GGA)	pepV	Dipeptidase
Reads supporting (aligned to +/- strand): ref base T (0/1); major base C (541/498); minor base A (4/0); total (545/499)
Fisher's exact test for biased strand distribution p-value = 1.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1050 total aligned reads displayed.

GTTCAACTTCAACAACCCCATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTTCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTAAGATAAGTTGCTCCAT  >  AM406671/1680891‑1681181

   caacttcaacAACCCCATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGTTTTTACCTTCACCATCTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATc                                                                                                                                             >  1:7452289/1‑149 (MQ=255)

      cttcaacAACCCCATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAAt                                                                                                                                          >  1:8096119/1‑149 (MQ=255)

      cttcaacAACCCCATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAAt                                                                                                                                          >  2:6355392/1‑149 (MQ=255)

       ttcaacAACCCCATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGTAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAAtt                                                                                                                                         <  2:5802294/149‑1 (MQ=255)

       ttcaacAACCCCATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAGACACCAGCATTCATTGAGGTATTTCCCATCAAtt                                                                                                                                         <  2:686986/149‑1 (MQ=255)

       ttcaacAACCCCATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAAtt                                                                                                                                         <  1:8990916/149‑1 (MQ=255)

       ttcaacAACCCCATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAAtt                                                                                                                                         <  2:8312154/149‑1 (MQ=255)

        tcaacAACCCCATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATtc                                                                                                                                        >  2:399363/1‑149 (MQ=255)

           acaacCCCATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATtcatc                                                                                                                                     <  1:3811420/149‑1 (MQ=255)

            caacCCCATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATtcatca                                                                                                                                    >  2:3484543/1‑149 (MQ=255)

             aacCCCATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATcaa                                                                                                                                   <  2:9585360/149‑1 (MQ=255)

               ccccATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACAGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATcaaca                                                                                                                                 >  1:6127856/1‑149 (MQ=255)

                 ccATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACaaa                                                                                                                               <  1:100085/149‑1 (MQ=255)

                  cATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACaaaa                                                                                                                              >  2:2361420/1‑149 (MQ=255)

                    tccaGTTTAGCAAGAATTTCGTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGc                                                                                                                            <  2:1964550/146‑1 (MQ=255)

                     cAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCt                                                                                                                           <  1:3352597/149‑1 (MQ=255)

                           tAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCt                                                                                                                     >  1:4950024/1‑149 (MQ=255)

                            aGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGAAAACGGAAATTGAGGGCGATTTTACCTTCACCCTTTTCATCAAATGACCAAACACCAGCATGCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTa                                                                                                                    <  2:7514732/149‑1 (MQ=255)

                             gCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTaa                                                                                                                   <  2:9749521/149‑1 (MQ=255)

                              cAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAAt                                                                                                                  <  1:4402607/149‑1 (MQ=255)

                                aGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAGAAGCTGTACCTAAttt                                                                                                                >  1:7061047/1‑149 (MQ=255)

                                aGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAAttt                                                                                                                >  2:1183423/1‑149 (MQ=255)

                                  aaTTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAAttttt                                                                                                              >  1:6462924/1‑149 (MQ=255)

                                       cTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCt                                                                                                         >  1:844563/1‑149 (MQ=255)

                                         tGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTc                                                                                                       <  1:955600/149‑1 (MQ=255)

                                          gCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCa                                                                                                      >  1:1605245/1‑149 (MQ=255)

                                            aTACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATg                                                                                                    <  1:9834732/149‑1 (MQ=255)

                                            aTACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATg                                                                                                    <  1:7296254/149‑1 (MQ=255)

                                             tACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGa                                                                                                   >  2:8646409/1‑149 (MQ=255)

                                              aCGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGAt                                                                                                  <  2:8026534/149‑1 (MQ=255)

                                                  tctGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTc                                                                                              >  1:6716118/1‑149 (MQ=255)

                                                   ctGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCa                                                                                             <  1:2281194/149‑1 (MQ=255)

                                                         tGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGt                                                                                       <  1:6350469/149‑1 (MQ=255)

                                                          gTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGtt                                                                                      <  2:776120/149‑1 (MQ=255)

                                                           ttCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGttt                                                                                     <  2:6738200/149‑1 (MQ=255)

                                                              ccTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTc                                                                                  >  2:1398098/1‑149 (MQ=255)

                                                              ccTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTc                                                                                  >  2:6507505/1‑149 (MQ=255)

                                                                ttGTGGTAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAg                                                                                <  2:6126901/149‑1 (MQ=255)

                                                                   tgGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATTAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGCTCTTCATccc                                                                             >  2:811911/1‑149 (MQ=255)

                                                                       aaaCGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAAc                                                                         <  1:9450494/149‑1 (MQ=255)

                                                                        aaCGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACt                                                                        <  1:7899183/149‑1 (MQ=255)

                                                                          cGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACttt                                                                      >  1:8772960/1‑149 (MQ=255)

                                                                             aaaTTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAAt                                                                   >  1:9109616/1‑149 (MQ=255)

                                                                                   aGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGgc                                                             >  2:8858701/1‑149 (MQ=255)

                                                                                   aGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGgc                                                             <  1:8604465/149‑1 (MQ=255)

                                                                                   aGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGgc                                                             >  2:7130640/1‑149 (MQ=255)

                                                                                     ggCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGgcag                                                           <  1:3623919/149‑1 (MQ=255)

                                                                                     ggCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGgcag                                                           <  1:2914102/149‑1 (MQ=255)

                                                                                      gCGATTCTACCTTCAGCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCAGTACCTAATTTTTCACCTTCGTGATCTTCAAGAAGTTTTTCAGCCGCAACTTTAATGAAGgcagc                                                          >  2:5104813/1‑149 (MQ=255)

                                                                                       cGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGgcagca                                                         <  1:3144144/149‑1 (MQ=255)

                                                                                       cGATCTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTCTTTCAGCCCCAACTTTAATGAAGgcagca                                                         <  2:4668309/149‑1 (MQ=255)

                                                                                          ttttACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGgcagcagca                                                      <  1:5623783/149‑1 (MQ=255)

                                                                                          ttttACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGgcagcagca                                                      <  1:5469304/149‑1 (MQ=255)

                                                                                          ttttACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGgcagcagca                                                      <  1:9307176/149‑1 (MQ=255)

                                                                                          ttttACCTTCACAATTTTCATCAGATGACCAAACACCAGCATTGATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGAACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCAACAACTTTAATGAAGgcagcagca                                                      >  2:2129823/1‑149 (MQ=255)

                                                                                           tttACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCAc                                                     >  1:1451485/1‑149 (MQ=255)

                                                                                            ttACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCAcc                                                    <  2:4112693/149‑1 (MQ=255)

                                                                                            ttACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCAcc                                                    >  2:3771112/1‑149 (MQ=255)

                                                                                              aCCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGGGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGt                                                  >  1:10197686/1‑149 (MQ=255)

                                                                                                cTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCa                                                >  2:3881080/1‑149 (MQ=255)

                                                                                                cTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCa                                                <  1:3470912/149‑1 (MQ=255)

                                                                                                  tCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGc                                              >  1:239826/1‑149 (MQ=255)

                                                                                                  tCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGc                                              >  2:7245697/1‑149 (MQ=255)

                                                                                                   cACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCa                                             <  2:48547/149‑1 (MQ=255)

                                                                                                      cATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCTGCCCTTACTTTAATGAAGGCAGCAGCACCGTCAGCAAAg                                          >  2:10069843/1‑149 (MQ=255)

                                                                                                       aTTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGt                                         >  1:1843978/1‑149 (MQ=255)

                                                                                                        ttttCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTc                                        <  1:5756714/149‑1 (MQ=255)

                                                                                                         tttCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCa                                       >  2:3194125/1‑149 (MQ=255)

                                                                                                         tttCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCa                                       >  2:9451979/1‑149 (MQ=255)

                                                                                                         tttCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCa                                       <  2:5633803/149‑1 (MQ=255)

                                                                                                         tttCAGCAGATGACCAAACACCAGCTTTCATTGAGTTATTTCCCATCGATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCa                                       <  2:5460462/149‑1 (MQ=255)

                                                                                                             atcaAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAAtt                                   >  2:9318170/1‑149 (MQ=255)

                                                                                                               caAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGa                                 <  2:2802974/149‑1 (MQ=255)

                                                                                                               caAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGa                                 >  1:1114369/1‑149 (MQ=255)

                                                                                                               caAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGa                                 <  1:1340076/149‑1 (MQ=255)

                                                                                                               caAATGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGa                                 <  2:4471290/149‑1 (MQ=255)

                                                                                                                   tGACCAAACACCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCa                             <  1:5899079/149‑1 (MQ=255)

                                                                                                                          acacCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACaa                      <  1:6874832/149‑1 (MQ=255)

                                                                                                                          acacCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACaa                      <  2:735899/149‑1 (MQ=255)

                                                                                                                          acacCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACaa                      <  2:4334118/149‑1 (MQ=255)

                                                                                                                          acacCAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACaa                      >  1:260216/1‑149 (MQ=255)

                                                                                                                              cAGCATTCATTGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATGTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTa                  >  2:2935179/1‑149 (MQ=255)

                                                                                                                              cAGCATTCATTGACGTATGTCCCATCAATTCATCAACAAAAGCTGTACCTATTTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTa                  <  1:5116270/149‑1 (MQ=255)

                                                                                                                                 cattcattGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTAAGa               <  1:8256788/149‑1 (MQ=255)

                                                                                                                                   ttcattGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAAGAAAGTAAGATa             <  1:5327805/149‑1 (MQ=255)

                                                                                                                                   ttcattGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTAAGATa             >  2:1712798/1‑149 (MQ=255)

                                                                                                                                    tcattGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTAAGATaa            >  2:522622/1‑149 (MQ=255)

                                                                                                                                    tcattGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTAAGATaa            >  1:4226805/1‑149 (MQ=255)

                                                                                                                                      attGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTGAGCAAAGTCAAATTGATTCAAGAACAAAGTAAGATAAGt          >  1:7603577/1‑149 (MQ=255)

                                                                                                                                      attGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTAAGATAAGt          >  2:10237586/1‑149 (MQ=255)

                                                                                                                                       ttGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCTCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTATGATAAGtt         >  2:2541493/1‑149 (MQ=255)

                                                                                                                                        tGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTAAGATAAGTTg        >  1:4046223/1‑149 (MQ=255)

                                                                                                                                        tGAGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTAAGATAAGTTg        >  2:1508400/1‑149 (MQ=255)

                                                                                                                                          aGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACTAAGTAAGATAAGTTGCt      <  2:5374675/149‑1 (MQ=255)

                                                                                                                                          aGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTAAGATAAGTTGCt      >  1:9668173/1‑149 (MQ=255)

                                                                                                                                          aGGTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTAAGATAAGTTGCt      <  1:7773921/149‑1 (MQ=255)

                                                                                                                                           ggTATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTAAGATAAGTTGCTc     >  2:9077596/1‑149 (MQ=255)

                                                                                                                                             tATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTAAGATAAGTTGCTCCa   >  1:4675869/1‑149 (MQ=255)

                                                                                                                                             tATTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTAAGATAAGTTGCTCCa   >  2:6236370/1‑149 (MQ=255)

                                                                                                                                              aTTTCCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTAAGATAAGTTGCTCCAt  >  2:8559515/1‑149 (MQ=255)

GTTCAACTTCAACAACCCCATCAAGTTTAGCAAGAATTTCTTGCATACGCTCTGGGCTGTTCCCTTGTGGGAAACGGAAATTGAGGGCGATTTTACCTTCACCATTTTCATCAAATGACCAAACACCAGCATTCATTGAGGTATTTTCCATCAATTCATCAACAAAAGCTGTACCTAATTTTTCACCTTCATGATCTTCAAGAAGTTTTTCAGCCCCAACTTTAATGAAGGCAGCAGCACCGTCAGCAAAGTCAAATTGATTCAAGAACAAAGTAAGATAAGTTGCTCCAT  >  AM406671/1680891‑1681181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`