Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 1,783,142 G→A A603V (GCA→GTA)  llmg_1803 ← putative secreted protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM4066711,783,1420GA99.9% 3347.3 / NA 886A603V (GCA→GTA) llmg_1803putative secreted protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (424/460);  total (424/462)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 886 total aligned reads displayed.

GGATTTTATTAGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTGCCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACTCGTGCCATTAGTGATAGACG  >  AM406671/1782996‑1783286
                                                                                                                                                  |                                                                                                                                                
 gATTTTATTAGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCg                                                                                                                                               >  1:2361786/1‑149 (MQ=255)
  aTTTTATTAGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGt                                                                                                                                              <  1:6115137/149‑1 (MQ=255)
  aTTTTATTAGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGt                                                                                                                                              <  2:1828106/149‑1 (MQ=255)
  aTTTTATTAGCCACTGTCGCTGGGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAAGTATAGCAGTCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAGGGTTACCGt                                                                                                                                              <  2:4839865/149‑1 (MQ=255)
   ttttATTAGCCACTGTTGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGCCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCGCCTGAGACGTAGCCATAGTTGAAGGTTACCGtt                                                                                                                                             <  1:7054197/149‑1 (MQ=255)
    tttATTAGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGttt                                                                                                                                            <  1:3267258/149‑1 (MQ=255)
    tttATTAGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCAGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGttt                                                                                                                                            >  1:6274784/1‑149 (MQ=255)
     ttattaGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTtg                                                                                                                                           <  2:4463430/149‑1 (MQ=255)
      tattaGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTtgt                                                                                                                                          >  2:8301740/1‑149 (MQ=255)
      tattaGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTGGAAGGTTACCGTTtgt                                                                                                                                          >  2:1279695/1‑149 (MQ=255)
       attaGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTtgtg                                                                                                                                         >  1:422637/1‑149 (MQ=255)
         taGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGtgct                                                                                                                                       >  2:10204138/1‑149 (MQ=255)
         taGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGCGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGtgct                                                                                                                                       >  2:9442877/1‑149 (MQ=255)
           gCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGtgctgc                                                                                                                                     >  2:9820472/1‑149 (MQ=255)
           gCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGtgctgc                                                                                                                                     >  2:9249115/1‑149 (MQ=255)
            ccACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGtgctgct                                                                                                                                    >  2:4133186/1‑149 (MQ=255)
             cACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGtgctgctg                                                                                                                                   <  1:104179/149‑1 (MQ=255)
              aCTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGtgctgctgc                                                                                                                                  >  2:3883175/1‑149 (MQ=255)
               cTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTgctgctgct                                                                                                                                 <  1:6374173/149‑1 (MQ=255)
                tGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGctgctgctg                                                                                                                                >  2:758681/1‑149 (MQ=255)
                tGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGctgctgctg                                                                                                                                >  1:5016290/1‑149 (MQ=255)
                  tCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTc                                                                                                                              >  1:1570513/1‑149 (MQ=255)
                   cGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCa                                                                                                                             <  1:3439142/149‑1 (MQ=255)
                   cGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGCGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCa                                                                                                                             >  2:5681193/1‑149 (MQ=255)
                       gtgGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGt                                                                                                                         >  2:8806192/1‑149 (MQ=255)
                        tgGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTg                                                                                                                        >  1:9541247/1‑149 (MQ=255)
                          gATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGAc                                                                                                                      >  1:7187933/1‑149 (MQ=255)
                            tGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCg                                                                                                                    <  2:6738535/149‑1 (MQ=255)
                            tGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCg                                                                                                                    <  1:8395050/149‑1 (MQ=255)
                             gCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGt                                                                                                                   >  1:1762747/1‑149 (MQ=255)
                              cTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGta                                                                                                                  >  2:3312949/1‑149 (MQ=255)
                                   ttCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGtt                                                                                                             <  2:6552479/149‑1 (MQ=255)
                                   ttCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGtt                                                                                                             >  1:9080207/1‑149 (MQ=255)
                                   ttCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGtt                                                                                                             <  2:2563449/149‑1 (MQ=255)
                                    tCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTa                                                                                                            <  2:3464367/149‑1 (MQ=255)
                                    tCCAACCAGGTGGTGGTGTGACATTTGCTCCTGTCGTGCCACGCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGTGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTa                                                                                                            <  2:8172427/149‑1 (MQ=255)
                                     ccagccagTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCTCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTAt                                                                                                           <  2:4660567/149‑1 (MQ=255)
                                         ccagTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGtt                                                                                                       >  1:8704408/1‑149 (MQ=255)
                                         ccagTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGtt                                                                                                       <  1:3589114/149‑1 (MQ=255)
                                            ggtggtggTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGc                                                                                                    <  1:2969187/147‑1 (MQ=255)
                                            gTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGc                                                                                                    >  2:3999960/1‑149 (MQ=255)
                                              tggtggTGTGGCAGTTGCTCCTGTCGTCCCACTCACTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGTTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCtt                                                                                                  <  2:1649617/149‑1 (MQ=255)
                                                gtggtgTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGtgc                                                                                                                                        <  1:4804046/109‑1 (MQ=255)
                                                gtggtgTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTgg                                                                                                <  1:2061658/149‑1 (MQ=255)
                                                gtggtgTGACACTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTgg                                                                                                >  2:8523884/1‑149 (MQ=255)
                                                   gtgtGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCGGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGtt                                                                                             <  1:7399907/149‑1 (MQ=255)
                                                    tgtgACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTg                                                                                            <  2:7145601/149‑1 (MQ=255)
                                                    tgtgACATTTGCTCCTGTCGGCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTg                                                                                            <  2:1211255/149‑1 (MQ=255)
                                                      tgACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTgcg                                                                                          >  2:494438/1‑149 (MQ=255)
                                                      tgACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTgcg                                                                                          <  2:2992710/149‑1 (MQ=255)
                                                       gACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTgcgc                                                                                         <  2:9040550/149‑1 (MQ=255)
                                                        aCATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCt                                                                                        >  1:5234213/1‑149 (MQ=255)
                                                         cATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTa                                                                                       >  1:8951923/1‑149 (MQ=255)
                                                          aTTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTaa                                                                                      <  2:276241/149‑1 (MQ=255)
                                                                tCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTgttg                                                                                >  1:3996276/1‑149 (MQ=255)
                                                                 ccTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTgttgg                                                                               >  2:10070764/1‑149 (MQ=255)
                                                                  cTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTgttggg                                                                              <  2:10386144/149‑1 (MQ=255)
                                                                   tGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTgttgggt                                                                             >  1:6683253/1‑149 (MQ=255)
                                                                    gtcgtcCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTgttgggtt                                                                            <  2:785381/149‑1 (MQ=255)
                                                                    gtcgtcCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTgttgggtt                                                                            >  1:5520368/1‑149 (MQ=255)
                                                                            aCTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCtg                                                                    >  1:5365920/1‑149 (MQ=255)
                                                                            aCTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCtg                                                                    >  2:4229764/1‑149 (MQ=255)
                                                                              tCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGTCGCtgtg                                                                  >  2:7738374/1‑149 (MQ=255)
                                                                              tCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCtgtg                                                                  <  2:2389969/149‑1 (MQ=255)
                                                                                gCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGaa                                                                <  1:5707225/149‑1 (MQ=255)
                                                                                  tGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAg                                                              >  1:2480327/1‑149 (MQ=255)
                                                                                   gACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGt                                                             >  1:8462185/1‑149 (MQ=255)
                                                                                    aCAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGtt                                                            >  1:10124395/1‑149 (MQ=255)
                                                                                      aGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGc                                                          <  1:3142479/149‑1 (MQ=255)
                                                                                        gCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCa                                                        >  1:9387124/1‑149 (MQ=255)
                                                                                        gCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCa                                                        >  2:527745/1‑149 (MQ=255)
                                                                                         cTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAg                                                       <  2:6242894/149‑1 (MQ=255)
                                                                                         cTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAg                                                       >  2:5443510/1‑149 (MQ=255)
                                                                                          tagtagGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAgt                                                      >  1:4645810/1‑149 (MQ=255)
                                                                                          tagtagGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAgt                                                      <  1:3747108/149‑1 (MQ=255)
                                                                                           agtagGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAgtg                                                     >  1:2722543/1‑149 (MQ=255)
                                                                                           agtagGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAgtg                                                     <  2:3689120/149‑1 (MQ=255)
                                                                                           agtagGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGGTGGGTTGGCCGCTGTGAAAGTTGCCAgtg                                                     >  2:3175944/1‑149 (MQ=255)
                                                                                              agGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCg                                                  <  1:1173409/149‑1 (MQ=255)
                                                                                                gTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTCTTGGGTTGGCCGCTGTGAAAGTTGCTAGTGTCGCt                                                >  1:9801289/1‑149 (MQ=255)
                                                                                                   taGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCa                                             <  2:2055011/149‑1 (MQ=255)
                                                                                                   taGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCa                                             <  1:1443313/149‑1 (MQ=255)
                                                                                                    aGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAg                                            >  1:4431504/1‑149 (MQ=255)
                                                                                                    aGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAg                                            <  2:5932436/149‑1 (MQ=255)
                                                                                                       aGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTg                                         <  2:6951408/149‑1 (MQ=255)
                                                                                                             gtTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGTGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCAc                                   <  2:1447887/149‑1 (MQ=255)
                                                                                                              ttGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCAcc                                  >  2:5130386/1‑149 (MQ=255)
                                                                                                              ttGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCAcc                                  <  1:7742821/149‑1 (MQ=255)
                                                                                                                     cTCCTGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATaa                           >  1:10001889/1‑149 (MQ=255)
                                                                                                                         tGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTAc                       <  2:7996506/149‑1 (MQ=255)
                                                                                                                         tGAGACGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTAc                       >  1:7538353/1‑149 (MQ=255)
                                                                                                                          gagaCGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACt                      <  2:2260068/149‑1 (MQ=255)
                                                                                                                           agaCGTATCCATAGGTGAAGTTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGAGTGGGTTGCGGTAACTGTTGGGTTGGTCGGTGTGAAAGTTGCTAGTGTCGCTGCAGTTGTGGCACCTGTATAAGTCCTc                     >  2:72665/1‑149 (MQ=255)
                                                                                                                             aCGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTNCAGTTGTCGCACCAGTATAAGTACTCGt                   <  1:5837880/149‑1 (MQ=255)
                                                                                                                              cGTAGCCATAGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACTCGTg                  <  1:5947812/149‑1 (MQ=255)
                                                                                                                                       aGTTGAAGGTTACCGTTTGTGCTGCTGCTGTCAGAGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTGGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCGCTAGTATAAGTACTCGTGCCATTAGTg         >  1:812932/1‑149 (MQ=255)
                                                                                                                                          tGAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACTCGTGCCATTAGTGATa      >  1:1281272/1‑149 (MQ=255)
                                                                                                                                           gAAGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACTCGTGCCATTAGTGATAg     <  1:6885056/149‑1 (MQ=255)
                                                                                                                                             aGGTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACTCGTGCCATTAGTGATAGAc   <  1:7904325/149‑1 (MQ=255)
                                                                                                                                              ggTTACCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACTCGTGCCATTAGTGATAGACg  >  2:967347/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GGATTTTATTAGCCACTGTCGCTGTGGATGCTGTATTCCAGCCAGTTGGTGGTGTGACATTTGCTCCTGTCGTCCCACTCGCTGACAGGCTAGTAGGTATAGCAGGCGTGTTGAGTGCTCCTGAGACGTAGCCATAGTTGAAGGTTGCCGTTTGTGCTGCTGCTGTCAGCGTGACCGTATAGTTATTGTTTGCTTGGGTTGCGCTAACTGTTGGGTTGGCCGCTGTGAAAGTTGCCAGTGTCGCTGCAGTTGTCGCACCAGTATAAGTACTCGTGCCATTAGTGATAGACG  >  AM406671/1782996‑1783286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑