BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	1,817,902	C→A	E463D (GAG→GAT)	xylS ←	alpha‑xylosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	1,817,902	0	C	A	99.8%	3808.7 / NA	985	E463D (GAG→GAT)	xylS	alpha‑xylosidase
Reads supporting (aligned to +/- strand): ref base C (2/0); new base A (518/465); total (520/465)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 999 total aligned reads displayed.

GGATATTCATAGTAGAGTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGCTCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCATGGCTCCACCAAGTATAGCCAA  >  AM406671/1817756‑1818047

    atTCATAGTAGAGTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTa                                                                                                                                             <  1:4228306/149‑1 (MQ=255)

       cATAGTAGAGTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGCTCTTTACTa                                                                                                                                          >  2:9415044/1‑149 (MQ=255)

         tagtagAGTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGt                                                                                                                                        >  1:8781395/1‑149 (MQ=255)

         tagtagAGTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGt                                                                                                                                        >  2:8733062/1‑149 (MQ=255)

         tagtagAGTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGCATCATTACTAGt                                                                                                                                        >  1:1039745/1‑149 (MQ=255)

          agtagAGTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTg                                                                                                                                       <  1:1916080/149‑1 (MQ=255)

             agagTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAAt                                                                                                                                    <  2:8826266/149‑1 (MQ=255)

              gagTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAAtt                                                                                                                                   >  1:6579783/1‑149 (MQ=255)

                gTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCt                                                                                                                                 >  2:8057312/1‑149 (MQ=255)

                   gTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGAt                                                                                                                              <  2:3408859/149‑1 (MQ=255)

                       aacaaGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTg                                                                                                                          >  1:4724472/1‑149 (MQ=255)

                         caaGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAg                                                                                                                        >  2:1783970/1‑149 (MQ=255)

                          aaGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAgg                                                                                                                       <  1:10035424/149‑1 (MQ=255)

                           aGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGa                                                                                                                      >  1:6426414/1‑149 (MQ=255)

                           aGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGa                                                                                                                      <  1:3109830/149‑1 (MQ=255)

                            gTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGaa                                                                                                                     <  1:8053924/149‑1 (MQ=255)

                            gTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGaa                                                                                                                     >  2:10048497/1‑149 (MQ=255)

                             tGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAAt                                                                                                                    >  1:8424956/1‑149 (MQ=255)

                              gCTCTACATTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTAATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATg                                                                                                                   <  1:114626/149‑1 (MQ=255)

                                tctACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGaa                                                                                                                 >  1:1398282/1‑149 (MQ=255)

                                 ctACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAg                                                                                                                <  1:9286509/149‑1 (MQ=255)

                                 ctACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAg                                                                                                                <  1:7174943/149‑1 (MQ=255)

                                  tACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGt                                                                                                               >  2:4613086/1‑149 (MQ=255)

                                  tACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGt                                                                                                               >  1:7024039/1‑149 (MQ=255)

                                         aGAAGCTGTTATTAAATTCGCGCTATAAAGATATGGAATTAATTGATGACCAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGAGCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCgattga                                                                                                        <  2:6606604/149‑1 (MQ=255)

                                          gAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCgattgat                                                                                                       >  2:590956/1‑149 (MQ=255)

                                            aGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGattgattg                                                                                                     <  2:5522213/149‑1 (MQ=255)

                                            aGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGattgattg                                                                                                     >  2:925815/1‑149 (MQ=255)

                                             gCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATAACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTgg                                                                                                    <  2:6875359/149‑1 (MQ=255)

                                               tGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTg                                                                                                  >  2:2215654/1‑149 (MQ=255)

                                                 ttattaAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGaa                                                                                                >  1:897772/1‑149 (MQ=255)

                                                 ttattaAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGaa                                                                                                <  1:3408282/149‑1 (MQ=255)

                                                  tattaAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGaaa                                                                                               <  2:6093058/149‑1 (MQ=255)

                                                  tattaAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGaaa                                                                                               >  2:2728311/1‑149 (MQ=255)

                                                  tattaAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGaaa                                                                                               <  2:9583030/149‑1 (MQ=255)

                                                  tattaAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGaaa                                                                                               >  2:8578402/1‑149 (MQ=255)

                                                   attaAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGaaaa                                                                                              <  1:2076913/149‑1 (MQ=255)

                                                    ttaAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAAt                                                                                             >  2:7403821/1‑149 (MQ=255)

                                                      aaaTTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGATGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATAc                                                                                           >  2:7063141/1‑149 (MQ=255)

                                                       aaTTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATAcc                                                                                          >  1:7822122/1‑149 (MQ=255)

                                                       aaTTGGCGCTATAAAGATATGGAATTAATTGATGACGAAATTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATAcc                                                                                          <  2:3648521/149‑1 (MQ=255)

                                                         ttGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATAcccc                                                                                        >  2:4924013/1‑149 (MQ=255)

                                                         ttGGCGCTACAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGCTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATAcccc                                                                                        >  1:5432589/1‑149 (MQ=255)

                                                            gcgcTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCaaa                                                                                     >  1:2944377/1‑149 (MQ=255)

                                                             cgcTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGGCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGACTGATTGGTGAAAATACCCCAAAt                                                                                    <  2:5698849/149‑1 (MQ=255)

                                                                tataAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGGTTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGc                                                                                 >  1:4350729/1‑149 (MQ=255)

                                                                  taAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACTCCAAATTGCaa                                                                               <  1:5312063/149‑1 (MQ=255)

                                                                  taAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCaa                                                                               <  2:1039542/149‑1 (MQ=255)

                                                                  taAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCaa                                                                               >  1:10185103/1‑149 (MQ=255)

                                                                      gATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAaccac                                                                           >  2:5198103/1‑149 (MQ=255)

                                                                       atatGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAaccacc                                                                          <  1:3648014/149‑1 (MQ=255)

                                                                       atatGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAaccacc                                                                          >  1:1299759/1‑149 (MQ=255)

                                                                         atGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCtt                                                                        >  2:3076590/1‑149 (MQ=255)

                                                                          tGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTa                                                                       <  1:4965223/149‑1 (MQ=255)

                                                                            gAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTaaa                                                                     >  1:1025287/1‑149 (MQ=255)

                                                                             aattaattGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAAc                                                                    >  2:4305702/1‑149 (MQ=255)

                                                                             aattaattGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAAc                                                                    <  1:3265427/149‑1 (MQ=255)

                                                                              atcaattGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACt                                                                   >  2:10266676/4‑149 (MQ=255)

                                                                                    tgatgaCGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTc                                                             >  2:1549360/1‑149 (MQ=255)

                                                                                        gaCGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCa                                                         >  1:6821154/1‑149 (MQ=255)

                                                                                         aCGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCAt                                                        >  2:9860755/1‑149 (MQ=255)

                                                                                           gAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCt                                                      <  1:9752092/149‑1 (MQ=255)

                                                                                             aGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCttt                                                    >  2:2031349/1‑149 (MQ=255)

                                                                                             aGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCttt                                                    >  2:8329003/1‑149 (MQ=255)

                                                                                              gTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCGGGGATCTTTACTAGTGAATTCTGATTTTGGGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTg                                                   >  1:5740534/1‑149 (MQ=255)

                                                                                              gTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTg                                                   >  2:4059421/1‑149 (MQ=255)

                                                                                                  tAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAAc                                               <  1:2300857/149‑1 (MQ=255)

                                                                                                  tAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAAc                                               <  1:9940746/149‑1 (MQ=255)

                                                                                                  tAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAACGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAAc                                               >  2:1467830/1‑149 (MQ=255)

                                                                                                  tAAAAAGTCAATCATTGATCGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAAc                                               <  2:5088778/149‑1 (MQ=255)

                                                                                                  gaaaaaGTCAGTCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAAc                                               <  2:6343491/148‑1 (MQ=255)

                                                                                                   aaaaaGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAAcc                                              <  2:3943909/149‑1 (MQ=255)

                                                                                                    aaaaGTCAATCATTGATTGTTCAATCACCGCAGCAAAATGACAGGGATCTTTACTAGTGAAGTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCt                                             <  2:1864388/149‑1 (MQ=255)

                                                                                                       aGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGc                                          <  1:9515847/149‑1 (MQ=255)

                                                                                                       aGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGc                                          <  1:832593/149‑1 (MQ=255)

                                                                                                        gTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCa                                         >  1:1654724/1‑149 (MQ=255)

                                                                                                             tCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGc                                    <  1:4548298/149‑1 (MQ=255)

                                                                                                              cATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGcc                                   <  2:8483093/149‑1 (MQ=255)

                                                                                                              cATTGATTGTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGcc                                   >  1:7309467/1‑149 (MQ=255)

                                                                                                                ttgattgTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATGGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGcccc                                 <  2:9140035/149‑1 (MQ=255)

                                                                                                                  gattgTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGcccccc                               >  1:6196921/1‑149 (MQ=255)

                                                                                                                   attgTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCa                              <  2:1295876/149‑1 (MQ=255)

                                                                                                                    ttgTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCaa                             >  1:2579825/1‑149 (MQ=255)

                                                                                                                     tgTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAat                            >  2:7611682/1‑149 (MQ=255)

                                                                                                                     tgTTCAATCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAat                            >  2:4822975/1‑149 (MQ=255)

                                                                                                                      gTTCAATCACCGCGTCAAATTGCCAGGGATCTTTACTAGTGAATTCTGATTGTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAata                           <  1:5105614/149‑1 (MQ=255)

                                                                                                                          aaTCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCAt                       >  1:5597701/1‑149 (MQ=255)

                                                                                                                          aaTCACCGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCAt                       <  1:245106/149‑1 (MQ=255)

                                                                                                                                cGCATCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCATGGCTcc                 <  1:7475556/149‑1 (MQ=255)

                                                                                                                                   aTCAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCATGGCTccacc              >  2:7908758/1‑149 (MQ=255)

                                                                                                                                     cAAAATGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCATGGCTCCACCaa            <  1:5988969/149‑1 (MQ=255)

                                                                                                                                      aaaaTGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCAGGTGCCCCCCAATATCATGGCTCCACCAAg           <  2:6473378/149‑1 (MQ=255)

                                                                                                                                       aaaTGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCATGGCTCCACCAAGt          >  1:1543601/1‑149 (MQ=255)

                                                                                                                                          tGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCATGGCTCCACCAAGtata       >  1:2816886/1‑149 (MQ=255)

                                                                                                                                          tGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCATGGCTCCACCAAGtata       >  1:3897168/1‑149 (MQ=255)

                                                                                                                                          tGCCAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCATGGCTCCACCAAGtata       >  2:2548264/1‑149 (MQ=255)

                                                                                                                                            ccAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCATGGCTCCACCAAGTATAGc     <  1:7657685/149‑1 (MQ=255)

                                                                                                                                            ccAGGGATCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCATGGCTCCACCAAGTATAGc     <  2:1171145/149‑1 (MQ=255)

                                                                                                                                              aGGGATCTTTACTAGTAAATTCTGATTTTAAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACAACATTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGACCCCCAATATCATGGCTCCACCAAGTATAGCCa   <  2:7220466/149‑1 (MQ=255)

                                                                                                                                               gggATCTTTACTAGCGAATTCTGATTTTGAGGAATGAAGTCGATGGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCATGGCTCCACCAAGTATAGCCaa  >  2:3216221/1‑149 (MQ=255)

GGATATTCATAGTAGAGTGGTTCAACAAGTGCTCTACCTTCAGAAGCTGTTATTAAATTGGCGCTATAAAGATATGGAATTAATTGATGACGAAGTTGTAAAAAGTCAATCATTGATTGTTCAATCACCGCATCAAAATGCCAGGGCTCTTTACTAGTGAATTCTGATTTTGAGGAATGAAGTCGATTGATTGGTGAAAATACCCCAAATTGCAACCACCTTAAACTGAGCTCCGCATCTTTGTAACCTTGCATGTGCCCCCCAATATCATGGCTCCACCAAGTATAGCCAA  >  AM406671/1817756‑1818047

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`