BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	1,854,998	T→A	V95V (GTA→GTT)	glgA ←	GlgA protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	1,854,998	0	T	A	99.8%	4677.6 / NA	1204	V95V (GTA→GTT)	glgA	GlgA protein
Reads supporting (aligned to +/- strand): ref base T (0/1); major base A (557/644); minor base C (2/0); total (559/645)
Fisher's exact test for biased strand distribution p-value = 2.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1206 total aligned reads displayed.

ACATGCAAAATGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTTACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCAACAGAAG  >  AM406671/1854852‑1855143

aCATGCAAAATGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAAc                                                                                                                                                 <  2:191647/149‑1 (MQ=255)

   tGCAAAATGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACAtt                                                                                                                                              <  2:7906656/149‑1 (MQ=255)

    gCAAAATGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTa                                                                                                                                             >  2:9485113/1‑149 (MQ=255)

      aaaaTGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATc                                                                                                                                           <  2:5150219/149‑1 (MQ=255)

      aaaaTGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATc                                                                                                                                           <  2:3676977/149‑1 (MQ=255)

       aaaTGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTACCATTATCt                                                                                                                                          >  2:261395/1‑149 (MQ=255)

       aaaTGTCAGGAATAAAATCTAATTGTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCt                                                                                                                                          <  2:2140492/149‑1 (MQ=255)

            tCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTAc                                                                                                                                     >  2:1134868/1‑149 (MQ=255)

               ggAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTaa                                                                                                                                  <  2:345230/149‑1 (MQ=255)

                gAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAg                                                                                                                                 <  2:1021209/149‑1 (MQ=255)

                      aaTCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAAAATTATCTTTTACTAAGGTTTTa                                                                                                                           >  2:6192679/1‑149 (MQ=255)

                       aTCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTaa                                                                                                                          <  1:2240872/149‑1 (MQ=255)

                         cTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATGACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACt                                                                                                                        >  1:6777574/1‑149 (MQ=255)

                           aaTTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTcc                                                                                                                      <  1:9717418/149‑1 (MQ=255)

                             tttttCTAAGAGCTGACAAACGGCTAAAGCGAAAAAGGCAAAACCTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCaa                                                                                                                    <  2:4737671/149‑1 (MQ=255)

                              ttttCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAAc                                                                                                                   >  1:8709555/1‑149 (MQ=255)

                                    aaGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTc                                                                                                             <  2:5641621/149‑1 (MQ=255)

                                     agagCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCa                                                                                                            >  1:4009408/1‑149 (MQ=255)

                                           gACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATgccgc                                                                                                      >  2:9321657/1‑149 (MQ=255)

                                           gACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATgccgc                                                                                                      >  2:6831214/1‑149 (MQ=255)

                                            aCAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTAATAAGGTTTTAACTCCAACATATTCATgccgcc                                                                                                     >  2:1823989/1‑149 (MQ=255)

                                              aaaCGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCaa                                                                                                   >  1:5502852/1‑149 (MQ=255)

                                               aaCGGCAAAATCGAAAAAGGCAAAAGGTTCTCCACCATCACCATAAAGGTAAACCTGTCCTCGACCACAATAATGCTCATTATCTAAAAGATAATATTTAACCTTATCTTTTACTAAGTCTTTAAATCCAACATATTCATGCCGCGAAc                                                                                                  >  2:1347162/1‑149 (MQ=255)

                                                aCGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAAcc                                                                                                 >  1:1104851/1‑149 (MQ=255)

                                                 cGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACGCCAACATATTCATGCCGCCAACCt                                                                                                <  1:7828286/149‑1 (MQ=255)

                                                   gCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTAc                                                                                              >  1:764339/1‑149 (MQ=255)

                                                    cTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACa                                                                                             >  2:8800563/1‑149 (MQ=255)

                                                      aaaTCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATc                                                                                           <  1:5008189/149‑1 (MQ=255)

                                                        aTCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATcaa                                                                                         <  1:4878597/149‑1 (MQ=255)

                                                        aTCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATcaa                                                                                         <  1:7439480/149‑1 (MQ=255)

                                                         tCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATcaac                                                                                        >  1:7967850/1‑149 (MQ=255)

                                                          cGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATcaaca                                                                                       >  1:1797916/1‑149 (MQ=255)

                                                            aaaaaGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACaaa                                                                                     <  2:3306657/149‑1 (MQ=255)

                                                              aaaGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAACTAATGCTCATTATCGAAAAAATAATATCTAACATTATCTTTTACTAAGGTTTTAACCCCAACATATTCATGCCGCCAACCTACATTAACAAAAt                                                                                   >  1:5361378/1‑149 (MQ=255)

                                                                  gCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATa                                                                               <  2:10051184/149‑1 (MQ=255)

                                                                  gCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATa                                                                               <  2:4074207/149‑1 (MQ=255)

                                                                       aCGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAAtt                                                                          <  1:2560710/149‑1 (MQ=255)

                                                                        cGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTc                                                                         <  2:4980710/149‑1 (MQ=255)

                                                                          ttCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCAt                                                                       <  2:1642547/149‑1 (MQ=255)

                                                                          ttCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCAt                                                                       >  1:9455653/1‑149 (MQ=255)

                                                                          gtctcCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATGATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCAt                                                                       <  2:2816512/148‑1 (MQ=255)

                                                                           tctcCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATc                                                                      >  2:4616324/1‑149 (MQ=255)

                                                                           tctcCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATc                                                                      >  2:3877190/1‑149 (MQ=255)

                                                                            ctcCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATAATAAAATTCATcg                                                                     >  2:4292136/1‑149 (MQ=255)

                                                                              ccATCACCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATcgac                                                                   <  2:9661561/149‑1 (MQ=255)

                                                                                   atcaCCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGagta                                                              >  1:3237728/1‑149 (MQ=255)

                                                                                    tcaCCATAACCGTAAATCTGCCCTCTACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGagtag                                                             <  2:7056798/149‑1 (MQ=255)

                                                                                    tcaCCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGagtag                                                             <  1:44925/149‑1 (MQ=255)

                                                                                        cATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGaa                                                         <  1:1367053/149‑1 (MQ=255)

                                                                                        cATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGaa                                                         >  1:121816/1‑149 (MQ=255)

                                                                                        cATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGaa                                                         <  1:8488065/149‑1 (MQ=255)

                                                                                         aTAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAAc                                                        >  1:6958126/1‑149 (MQ=255)

                                                                                           aaCCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCGGCCAACCTACATCAACAAAATCATCAAATTCATCGACGAGTAGTGAACGa                                                      >  2:5896564/1‑149 (MQ=255)

                                                                                           aaCCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCAt                                                                       >  2:10386399/1‑132 (MQ=255)

                                                                                           aaCCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCAt                                                                       <  1:10386399/132‑1 (MQ=255)

                                                                                           aaCCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGa                                                      <  2:9865493/149‑1 (MQ=255)

                                                                                              cGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGatat                                                   <  2:8429710/149‑1 (MQ=255)

                                                                                              cGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGatat                                                   <  2:2344969/149‑1 (MQ=255)

                                                                                                tAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATAttc                                                 <  2:7438407/149‑1 (MQ=255)

                                                                                                tAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATAttc                                                 >  1:3437115/1‑149 (MQ=255)

                                                                                                tAAATCTGCCCTCGACCAAAATAATGCTCATTAACTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATAttc                                                 <  2:9176184/149‑1 (MQ=255)

                                                                                                 aaaTCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATAttct                                                >  1:10002712/1‑149 (MQ=255)

                                                                                                  aaTCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATAttctt                                               >  1:1427650/1‑149 (MQ=255)

                                                                                                    tCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATtcttct                                             <  2:2935855/149‑1 (MQ=255)

                                                                                                    tCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATtcttct                                             >  2:1536275/1‑149 (MQ=255)

                                                                                                     cTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTcttctt                                            <  1:92250/149‑1 (MQ=255)

                                                                                                         ccTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCtttcat                                        <  1:3899964/149‑1 (MQ=255)

                                                                                                         ccTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCtttcat                                        >  1:2923913/1‑149 (MQ=255)

                                                                                                          cTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCtttcatt                                       <  1:7150189/149‑1 (MQ=255)

                                                                                                          cTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCtttcatt                                       <  1:1956774/149‑1 (MQ=255)

                                                                                                           tCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCtttcattt                                      <  1:6266165/149‑1 (MQ=255)

                                                                                                                cAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTcatttcattt                                 >  2:7666897/1‑149 (MQ=255)

                                                                                                                cAAAATAATGCTCATTATCTAAAAAATAATATTTAACATTATATTTTACTAAGTTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACTAGTAGTGAACGATATTCTTCTTTcatttcattt                                 >  2:7243669/1‑149 (MQ=255)

                                                                                                                aaatataatGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCCACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTcatttcattt                                 <  2:3094948/145‑1 (MQ=255)

                                                                                                                 aaaaTAATGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCAtttt                                >  2:8630679/1‑149 (MQ=255)

                                                                                                                   aataatGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGGGTAGTGAACGATATTCTTCTTTCATTTCATTTTTg                              >  1:572072/1‑149 (MQ=255)

                                                                                                                   aataatGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTg                              >  1:10149883/1‑149 (MQ=255)

                                                                                                                   aataatGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTg                              >  1:9635141/1‑149 (MQ=255)

                                                                                                                    ataatGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGa                             <  2:2525853/149‑1 (MQ=255)

                                                                                                                    ataatGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGa                             >  2:6329811/1‑149 (MQ=255)

                                                                                                                     taatGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGaa                            >  1:6429172/1‑149 (MQ=255)

                                                                                                                      aatGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGaaa                           <  1:8880187/149‑1 (MQ=255)

                                                                                                                        tGCTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATa                         >  1:912868/1‑149 (MQ=255)

                                                                                                                          cTCATTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAg                       <  1:3642126/149‑1 (MQ=255)

                                                                                                                             aTTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTa                    <  1:8158174/149‑1 (MQ=255)

                                                                                                                             aTTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTa                    >  1:4675119/1‑149 (MQ=255)

                                                                                                                             aTTATCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTGGAAATAAGGTa                    >  1:2788537/1‑149 (MQ=255)

                                                                                                                                aTCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTaaaa                 <  2:8150338/149‑1 (MQ=255)

                                                                                                                                aTCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTaaaa                 <  1:4182484/149‑1 (MQ=255)

                                                                                                                                 tCTAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAAt                <  1:5221118/149‑1 (MQ=255)

                                                                                                                                   tAAAAAATAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATca              >  1:986264/1‑149 (MQ=255)

                                                                                                                                    aaaaaaTAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATcac             <  2:9050517/149‑1 (MQ=255)

                                                                                                                                     aaaaaTAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATcaca            >  2:6082049/1‑149 (MQ=255)

                                                                                                                                     aaaaaTAATATTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATcaca            <  1:3088980/149‑1 (MQ=255)

                                                                                                                                         ataataTTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCaa        <  2:888186/149‑1 (MQ=255)

                                                                                                                                            atatTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATTTTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCAACag     <  2:5362065/149‑1 (MQ=255)

                                                                                                                                            atatTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCAACag     <  1:1624883/149‑1 (MQ=255)

                                                                                                                                            atatTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCAACag     >  1:6012869/1‑149 (MQ=255)

                                                                                                                                            atatTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCAACag     <  1:8382569/149‑1 (MQ=255)

                                                                                                                                             tatTTAACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCAACaga    >  1:2386509/1‑149 (MQ=255)

ACATGCAAAATGTCAGGAATAAAATCTAATTTTTCTAAGAGCTGACAAACGGCTAAATCGAAAAAGGCAAAACGTTCTCCATCATCACCATAACCGTAAATCTGCCCTCGACCAAAATAATGCTCATTATCTAAAAAATAATATTTTACATTATCTTTTACTAAGGTTTTAACTCCAACATATTCATGCCGCCAACCTACATCAACAAAATCATAAAATTCATCGACGAGTAGTGAACGATATTCTTCTTTCATTTCATTTTTGAAATAAGGTAAAATCACACCAACAGAAG  >  AM406671/1854852‑1855143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`