Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 617,310 A→C S8S (TCA→TCC)  llmg_0628 → divalent cation transport‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM406671617,3100AC94.7% 3988.9 / 10.5 1103S8S (TCA→TCC) llmg_0628divalent cation transport‑related protein
Reads supporting (aligned to +/- strand):  ref base A (4/54);  new base C (575/463);  total (583/520)
Fisher's exact test for biased strand distribution p-value = 2.31e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1111 total aligned reads displayed.

CTTTTCGTTAAGGCTTTGAAATAAAATAATGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCAAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAATTTTAGATGACTATGAAAATG  >  AM406671/617164‑617455
                                                                                                                                                  |                                                                                                                                                 
cTTTTCGTTAAGGCTTTGAAATAAAATAATGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCcaa                                                                                                                                                 >  1:1543678/1‑149 (MQ=255)
  tttCGTTAAGGCTTTGAAATAAAATAATGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATg                                                                                                                                               <  2:9719963/149‑1 (MQ=255)
  tttCGTTAAGGCTTTGAAATAAAATAATGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATg                                                                                                                                               <  1:8013102/149‑1 (MQ=255)
  tttCGTTAAGGCTTTGAAATAAAATAATGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATg                                                                                                                                               >  1:5746622/1‑149 (MQ=255)
       ttAAGGCTTTGAAATAAAATAATGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGaaaaa                                                                                                                                          <  1:4007339/149‑1 (MQ=255)
        tAAGGCTTTGAAATAAAATAATGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGaaaaaa                                                                                                                                         >  1:1161456/1‑149 (MQ=255)
           ggCTTTGAAATAAAATAATGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGATAAGAAGAGATATATAGGCTTATATTATAAAAAATTATGAAATATCTAATTAAAAAAAAt                                                                                                                                      >  2:9421975/1‑149 (MQ=255)
                tGAAATAAAATAATGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTg                                                                                                                                    <  1:10200937/146‑1 (MQ=255)
                tGAAATAAAATAATGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTg                                                                                                                                    >  2:10200937/1‑146 (MQ=255)
                tGAAATAAAATAATGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGAtc                                                                                                                                 >  1:5164138/1‑149 (MQ=255)
                  aaataaaataATGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGAtctc                                                                                                                               <  1:9447855/149‑1 (MQ=255)
                   aataaaataaTGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCa                                                                                                                              <  1:6252322/149‑1 (MQ=255)
                     taaaataaTGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAAc                                                                                                                            >  1:7416243/1‑149 (MQ=255)
                     taaaataaTGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAAc                                                                                                                            >  1:4208352/1‑149 (MQ=255)
                      aaaataaTGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCTTTTTTTTTTTTTTTTAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAATAAAATTGATCTCAACt                                                                                                                           >  2:2725276/1‑149 (MQ=255)
                      aaaataaTGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAATTATCCAATGAAAAAAAATTGATCTCAACt                                                                                                                           <  2:6473208/149‑1 (MQ=255)
                      aaaataaTGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATACAATGAAAAAAAATTGATCTCAACt                                                                                                                           >  1:1415753/1‑149 (MQ=255)
                        aataatGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACttt                                                                                                                         >  2:7697201/1‑148 (MQ=255)
                         ataatGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTTTTTTGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCt                                                                                                                        >  2:3898378/1‑149 (MQ=255)
                           aatGAGAAAAAAATAGGAAAAAAAGATAAAATTAGAAGGAGAAGAAGAGGATTTAAAACCTTTTTTATTAGGAAAAGAAGGGATAGATAGGATAAAATGATAAAAAATTATGAACTATCAAATGAAAAAAAATTGATCTCAACTTCTga                                                                                                                      <  2:9542007/149‑1 (MQ=255)
                            atGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTgag                                                                                                                     >  1:9092299/1‑149 (MQ=255)
                             tGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTgaga                                                                                                                    >  2:8096382/1‑149 (MQ=255)
                             tGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTgaga                                                                                                                    >  2:6817310/1‑149 (MQ=255)
                               agaAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGTTAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCAAATGAAAAAAAATTGATCTCAACTTATGAGATg                                                                                                                  >  2:5894038/1‑149 (MQ=255)
                               agaAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTGTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATg                                                                                                                  <  1:5006771/149‑1 (MQ=255)
                               agaAAAAAATAAGAAAATCAGCTACAATTAGAAGGAGAAGAAGAAGATTTAAAACCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAAAAAAAAAAAAAAAAAAAATTGATCTCAACTTCTGAGATg                                                                                                                  <  2:7169584/149‑1 (MQ=255)
                                gaAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATga                                                                                                                 >  2:8877502/1‑149 (MQ=255)
                                    aaaaTAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATgaagaa                                                                                                             >  1:2113916/1‑149 (MQ=255)
                                    aaaaTAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATgaagaa                                                                                                             <  2:9001744/149‑1 (MQ=255)
                                       aTAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAAttt                                                                                                          >  1:253191/1‑149 (MQ=255)
                                         aGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCa                                                                                                        >  1:9255948/1‑149 (MQ=255)
                                          ggAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTTTTTTTTTATGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTTATCTCAACTTCTGAGATGAAGAATTTCAc                                                                                                       >  2:7883968/1‑149 (MQ=255)
                                           gAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACt                                                                                                      <  2:6254083/149‑1 (MQ=255)
                                           gAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACt                                                                                                      >  1:7654383/1‑149 (MQ=255)
                                               aTCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTATGATAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATTCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTAACTTATg                                                                                                  >  2:2365721/1‑149 (MQ=255)
                                               aTCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATg                                                                                                  >  1:6897628/1‑149 (MQ=255)
                                                 cTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTc                                                                                                >  1:3151549/1‑149 (MQ=255)
                                                 cTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTCATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTGCTGAGATGAAGAATTTCACTTATGTc                                                                                                >  1:6707436/1‑149 (MQ=255)
                                                  tGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTcc                                                                                               >  1:3076991/1‑149 (MQ=255)
                                                  tGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCAAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTcc                                                                                               <  2:3486944/149‑1 (MQ=255)
                                                   gCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCt                                                                                              >  1:45558/1‑149 (MQ=255)
                                                     taaaaTTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGAAAATAAAAAAAAAAATTATAAACAATCAAATAAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCa                                                                                            <  2:5538743/146‑1 (MQ=255)
                                                     aaaaaTAAGAAAGAGAAGAAGAAGAATTAAAAACTTTTTTATTAGCAAAAGAAGAGATAGATAGGCTGATATGATAAAAAATTATGAACTATCAAATAAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCa                                                                                            <  2:726148/146‑1 (MQ=255)
                                                      aCAATTATAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGATGATATGATAAAAAATTATGAAAAAAAAAAAAAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCaa                                                                                           <  2:3283865/149‑1 (MQ=255)
                                                      aCAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCaa                                                                                           >  2:5265601/1‑149 (MQ=255)
                                                         aTTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTga                                                                                                                      <  2:5140425/119‑1 (MQ=255)
                                                          ttAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGATGATATGATAAAAAAATATGAACTATCCAATGAAAAAAAATTGATGACAACTTCTGAGCTGAAGAATATCACTTATGTCGTCAATCCa                                                                                       >  2:4536073/1‑149 (MQ=255)
                                                           aaGAAGGAAAAAAAAAGGATTTAAATCCATTTGTATTAGGAAAAAAACGGATAGATATGCTGATATGAAAAAAAATTATGAACTATCCAAAGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCaa                                                                                      <  2:4915025/148‑1 (MQ=255)
                                                                ggAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGt                                                                                 <  1:8549311/149‑1 (MQ=255)
                                                                   gaagaagaGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTgaa                                                                              >  1:5627677/1‑149 (MQ=255)
                                                                   gaagaagaGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTgaa                                                                              <  1:3564243/149‑1 (MQ=255)
                                                                    aagaagaGGATTTAAATCCTTTTTTTTTTTTTTTAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTgaag                                                                             >  2:10391560/1‑149 (MQ=255)
                                                                    aagaagaGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTgaag                                                                             <  2:4061281/149‑1 (MQ=255)
                                                                      gaagaGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTgaagaa                                                                           <  2:8463787/149‑1 (MQ=255)
                                                                      gaagaGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTgaagaa                                                                           >  2:4739285/1‑149 (MQ=255)
                                                                        agagGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCAAAAAAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAAt                                                                         <  2:871043/149‑1 (MQ=255)
                                                                           ggATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTgg                                                                      >  1:3752645/1‑149 (MQ=255)
                                                                             aTTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGa                                                                    >  1:4341488/1‑149 (MQ=255)
                                                                                tAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCACTCCAACAAGTGAAGAAATTGGGAata                                                                 >  1:4840484/1‑149 (MQ=255)
                                                                                tAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAata                                                                 >  2:2597042/1‑149 (MQ=255)
                                                                                 aaaTCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAatat                                                                <  1:3279003/149‑1 (MQ=255)
                                                                                 aaaTCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAatat                                                                >  2:1567025/1‑149 (MQ=255)
                                                                                 aaaTACTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAACAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAatat                                                                <  2:5366653/149‑1 (MQ=255)
                                                                                  aaTCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATAtc                                                               <  2:1904890/149‑1 (MQ=255)
                                                                                       ttttttATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGa                                                          >  1:8866903/1‑149 (MQ=255)
                                                                                       ttttttATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGa                                                          >  1:7868549/1‑149 (MQ=255)
                                                                                         ttttATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAac                                                        >  1:2884836/1‑149 (MQ=255)
                                                                                           ttattaGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAacac                                                      >  1:9735761/1‑149 (MQ=255)
                                                                                             attaGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTa                                                    >  1:5485712/1‑149 (MQ=255)
                                                                                               taGGAAAAGAAGGGATAGATAGGCTGATGTGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATg                                                  >  2:6092721/1‑149 (MQ=255)
                                                                                                aGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGa                                                 >  1:10002571/1‑149 (MQ=255)
                                                                                                  gAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTCTGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGAtt                                               <  1:6441363/149‑1 (MQ=255)
                                                                                                  gAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATGTCTCAGAACACTATGAtt                                               <  2:4333577/149‑1 (MQ=255)
                                                                                                   aaaaGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGAttt                                              >  2:2981263/1‑149 (MQ=255)
                                                                                                    aaaGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGAtttt                                             >  1:9582673/1‑149 (MQ=255)
                                                                                                        aagGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCtt                                         <  1:172502/149‑1 (MQ=255)
                                                                                                         agGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCttt                                        <  1:2369886/149‑1 (MQ=255)
                                                                                                          gggATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCtttt                                       <  2:2160209/149‑1 (MQ=255)
                                                                                                          gggATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCtttt                                       >  1:7279540/1‑149 (MQ=255)
                                                                                                           ggATAGATAGGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTg                                      >  2:6705714/1‑149 (MQ=255)
                                                                                                             atagatagGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTCTGATTTTCCTTTTGAc                                    >  1:7021469/1‑149 (MQ=255)
                                                                                                             atagatagGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGAc                                    >  1:2683218/1‑149 (MQ=255)
                                                                                                               agatagACTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGActa                                  <  1:6018187/149‑1 (MQ=255)
                                                                                                                  tagGCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCANAACACTATGATTTTCCTTTTGActatct                               <  1:5814006/149‑1 (MQ=255)
                                                                                                                  aagGCAGATATGAAAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGActatct                               <  2:1150781/148‑1 (MQ=255)
                                                                                                                    ggCTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGActatctat                             <  2:7501363/149‑1 (MQ=255)
                                                                                                                      ttgatatgataAAAAATTATGAAATATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTatctatct                           <  2:920495/148‑1 (MQ=255)
                                                                                                                      cTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTatctatct                           <  1:8193105/149‑1 (MQ=255)
                                                                                                                      cTGATATGATAAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTatctatct                           <  1:8666686/149‑1 (MQ=255)
                                                                                                                           atgataAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAAt                      >  1:8360280/1‑149 (MQ=255)
                                                                                                                             gataAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAAttt                    >  1:1952084/1‑149 (MQ=255)
                                                                                                                              ataAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAAtttt                   >  2:1413539/1‑149 (MQ=255)
                                                                                                                              ataAAAAATTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAAtttt                   <  1:5350706/149‑1 (MQ=255)
                                                                                                                              ataAAAAATTATGAACTATCCAATGAAAAAAAAATGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAAtttt                   >  1:9970117/1‑149 (MQ=255)
                                                                                                                                 aaaaaTTATGAACTATCCAATGAAATAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAATTTTAGa                >  2:5759852/1‑149 (MQ=255)
                                                                                                                                     aTTATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAATTTTAGATGAc            <  2:1791724/149‑1 (MQ=255)
                                                                                                                                     aTTATGAACAATAAAAAAAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAATTTTAGATGAc            <  2:3766356/149‑1 (MQ=255)
                                                                                                                                      ttATGAACTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAATTTTAGATGACt           >  2:8297038/1‑149 (MQ=255)
                                                                                                                                           aaCTATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAATTTTAGATGACTATGaa      <  1:10410326/149‑1 (MQ=255)
                                                                                                                                              tATCCAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAATTTTAGATGACTATGAAAAt   >  1:6817187/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CTTTTCGTTAAGGCTTTGAAATAAAATAATGAGAAAAAAATAGGAAAATCTGCTACAATTAGAAGGAGAAGAAGAGGATTTAAATCCTTTTTTATTAGGAAAAGAAGGGATAGATAGGCTGATATGATAAAAAATTATGAACTATCAAATGAAAAAAAATTGATCTCAACTTCTGAGATGAAGAATTTCACTTATGTCCTCAATCCAACACGTGAAGAAATTGGGAATATCTCAGAACACTATGATTTTCCTTTTGACTATCTATCTGGAATTTTAGATGACTATGAAAATG  >  AM406671/617164‑617455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑