BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	2,266,724	T→C	I10T (ATT→ACT)	argR →	arginine repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	2,266,724	0	T	C	99.9%	5626.8 / NA	1360	I10T (ATT→ACT)	argR	arginine repressor
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (678/680); total (679/681)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1384 total aligned reads displayed.

ACCATCGATTGCAGCAGACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTATTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAACGGGACAAAGGAGAG  >  AM406671/2266578‑2266869

   aTCGATTGCAGCAGACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAg                                                                                                                                              >  1:5246385/1‑149 (MQ=255)

     cGATTGCAGCAGACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGaa                                                                                                                                            <  1:8217303/149‑1 (MQ=255)

      gATTGCAGCAGACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGNGATAAAAGATTAGAAATTACTAAAGaaa                                                                                                                                           <  1:5793756/149‑1 (MQ=255)

           cagcagACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATtgtg                                                                                                                                      <  1:2012113/149‑1 (MQ=255)

           cagcagACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATtgtg                                                                                                                                      <  2:275850/149‑1 (MQ=255)

             gcagACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGAc                                                                                                                                    >  2:4600262/1‑149 (MQ=255)

                     tGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAataata                                                                                                                            <  1:1630163/149‑1 (MQ=255)

                       cTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATaaa                                                                                                                          >  2:896460/1‑149 (MQ=255)

                        ttGTCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATaaaa                                                                                                                         <  2:3994253/149‑1 (MQ=255)

                         tgtcTTACTAGTTGTTTTTTGTTCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAat                                                                                                                        <  2:10172948/146‑1 (MQ=255)

                          ggCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAata                                                                                                                       <  1:2708656/149‑1 (MQ=255)

                           gCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAatat                                                                                                                      <  1:10403136/149‑1 (MQ=255)

                            cTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATAtt                                                                                                                     <  1:6471200/149‑1 (MQ=255)

                             ttACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTg                                                                                                                    >  1:2911437/1‑149 (MQ=255)

                              tACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGa                                                                                                                   <  2:972796/149‑1 (MQ=255)

                               aCTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGAc                                                                                                                  >  1:7650958/1‑149 (MQ=255)

                               aCTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGAc                                                                                                                  >  1:8549639/1‑149 (MQ=255)

                                cTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACt                                                                                                                 <  1:8034433/149‑1 (MQ=255)

                                    ttgttTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCaag                                                                                                             <  1:9912217/149‑1 (MQ=255)

                                    ttgttTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCaag                                                                                                             >  1:750610/1‑149 (MQ=255)

                                       tttttCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAAGTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCaagaag                                                                                                          <  2:4777325/149‑1 (MQ=255)

                                          ttCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGc                                                                                                       >  1:2163614/1‑149 (MQ=255)

                                            cGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCtt                                                                                                     <  1:4347071/149‑1 (MQ=255)

                                            cGTCCATTCACTAATTATAAAGAAAAAACACTAATGTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCtt                                                                                                     <  2:5618135/149‑1 (MQ=255)

                                             ttacATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTc                                                                                                    <  2:8007714/146‑1 (MQ=255)

                                              tCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCa                                                                                                   <  1:6988470/149‑1 (MQ=255)

                                               ccATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTGTATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCaa                                                                                                  >  2:7529503/1‑149 (MQ=255)

                                               ccATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCaa                                                                                                  <  1:9091543/149‑1 (MQ=255)

                                               ccATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCaa                                                                                                  <  2:1188892/149‑1 (MQ=255)

                                                  ttCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAAtct                                                                                               >  1:8901957/1‑149 (MQ=255)

                                                      cTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCtat                                                                                           >  1:10045315/1‑149 (MQ=255)

                                                      cTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCtat                                                                                           >  1:102295/1‑149 (MQ=255)

                                                          ttATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTattatt                                                                                       >  1:6650146/1‑149 (MQ=255)

                                                           tataCAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTAttatta                                                                                      <  2:3269166/149‑1 (MQ=255)

                                                            ataCAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAg                                                                                     <  1:1304630/149‑1 (MQ=255)

                                                             taCAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGa                                                                                    <  1:3076854/149‑1 (MQ=255)

                                                                  aaaaaaCACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAAcgcg                                                                               <  1:6246440/149‑1 (MQ=255)

                                                                  aaaaaaCACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAAcgcg                                                                               >  2:3886734/1‑149 (MQ=255)

                                                                      aaCACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGgtgt                                                                           <  2:2335597/149‑1 (MQ=255)

                                                                        cacTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTag                                                                         >  2:5281884/1‑149 (MQ=255)

                                                                        cacTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTag                                                                         <  2:2541410/149‑1 (MQ=255)

                                                                        cacTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGCag                                                                         >  2:5858389/1‑149 (MQ=255)

                                                                           tAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAgg                                                                      >  2:683544/1‑149 (MQ=255)

                                                                              ttttGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTaa                                                                   >  2:3100020/1‑149 (MQ=255)

                                                                                  gTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAAcaca                                                               <  1:6822799/149‑1 (MQ=255)

                                                                                   tCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACaa                                                              <  2:9293377/149‑1 (MQ=255)

                                                                                     aaTATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGc                                                            >  1:5578126/1‑149 (MQ=255)

                                                                                      atatatAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAATcc                                                           <  2:4173693/149‑1 (MQ=255)

                                                                                      atatatAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGcc                                                           <  2:1368980/149‑1 (MQ=255)

                                                                                      atatatAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGcc                                                           >  1:4534117/1‑149 (MQ=255)

                                                                                      atatatAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGcc                                                           >  1:243945/1‑149 (MQ=255)

                                                                                      atatatAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAAcc                                                           >  1:1492642/1‑149 (MQ=255)

                                                                                        atataGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCAc                                                         >  2:4351928/1‑149 (MQ=255)

                                                                                        atataGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCAc                                                         >  2:8430930/1‑149 (MQ=255)

                                                                                          ataGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACtt                                                       >  2:730068/1‑149 (MQ=255)

                                                                                          ataGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACtt                                                       >  1:955452/1‑149 (MQ=255)

                                                                                           taGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACttt                                                      <  1:4085753/149‑1 (MQ=255)

                                                                                             gTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTAt                                                    >  1:9224385/1‑149 (MQ=255)

                                                                                              tCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATc                                                   <  2:9498029/149‑1 (MQ=255)

                                                                                              tCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATc                                                   >  1:7887168/1‑149 (MQ=255)

                                                                                              tCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATc                                                   <  2:7800958/149‑1 (MQ=255)

                                                                                                 aTTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCag                                                <  1:1808814/149‑1 (MQ=255)

                                                                                                  ttATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCaga                                               <  1:4771266/149‑1 (MQ=255)

                                                                                                  ttATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTATATTATTAGTACGCGGTGTAGAGGTAACACAAGCCACTTTATCCaga                                               >  2:2048517/1‑149 (MQ=255)

                                                                                                   tataTGGTATAATTATGCTATTAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGGAGCGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTATATCCagag                                              <  1:5421400/149‑1 (MQ=255)

                                                                                                   tataTGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCagag                                              >  1:8372428/1‑149 (MQ=255)

                                                                                                   tataTGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCGATTATTAGAACGCGGTGTAGATGTAACACAAGCCACTTTATCCagag                                              <  2:2224203/149‑1 (MQ=255)

                                                                                                    atatGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCTAgaga                                             >  2:6225660/1‑149 (MQ=255)

                                                                                                    atatGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAgaga                                             <  2:5042533/149‑1 (MQ=255)

                                                                                                     tatGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGat                                            >  2:1539485/1‑149 (MQ=255)

                                                                                                     tatGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGat                                            <  2:8785760/149‑1 (MQ=255)

                                                                                                     tatGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGat                                            >  1:3181498/1‑149 (MQ=255)

                                                                                                      atGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGata                                           >  2:7607952/1‑149 (MQ=255)

                                                                                                       tGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGatat                                          <  2:5432763/149‑1 (MQ=255)

                                                                                                         gTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTa                                        >  1:942348/1‑149 (MQ=255)

                                                                                                         gTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTa                                        <  2:296118/149‑1 (MQ=255)

                                                                                                           ataATTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGa                                      <  1:368581/149‑1 (MQ=255)

                                                                                                             aaTTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGaaa                                    >  2:8590168/1‑149 (MQ=255)

                                                                                                              aTTATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGaaaa                                   >  2:10334665/1‑149 (MQ=255)

                                                                                                                tATGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAAtt                                 >  1:5023956/1‑149 (MQ=255)

                                                                                                                  tGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTaa                               <  1:3318529/149‑1 (MQ=255)

                                                                                                                  tGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTaa                               >  1:4205079/1‑149 (MQ=255)

                                                                                                                  tGCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATGGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCAGTGTAGTGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTaa                               <  2:7045529/149‑1 (MQ=255)

                                                                                                                   gCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTaaa                              >  1:5951666/1‑149 (MQ=255)

                                                                                                                   gCTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTaaa                              >  2:3660351/1‑149 (MQ=255)

                                                                                                                    cTATGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAat                             <  1:3911963/149‑1 (MQ=255)

                                                                                                                      aTGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGACATATTAGAAAATTAAatat                           <  2:1881737/149‑1 (MQ=255)

                                                                                                                       tGAAAAGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTACCACAAGCCACTTTATCCAGAGATATTAGACAATTAAAtatt                          >  2:3473255/1‑149 (MQ=255)

                                                                                                                         aaaaGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAAtattat                        <  1:8695052/149‑1 (MQ=255)

                                                                                                                         aaaaGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAAtattat                        <  1:1137676/149‑1 (MQ=255)

                                                                                                                          aaaGAGATTAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATtata                       <  1:6047437/149‑1 (MQ=255)

                                                                                                                          aaaGAGATAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATtata                       >  2:819383/1‑149 (MQ=255)

                                                                                                                              aaaTAAAAAATAACAAATTACTAAAAAAATTGTGACAAATAATAAAATATTGACTCAATAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTATAAAATTAAATATTATaaaaa                   <  2:6037050/147‑1 (MQ=255)

                                                                                                                                aTAAAAGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATGTCTCTTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATaaaaaaa                 >  2:6587428/1‑149 (MQ=255)

                                                                                                                                    aaGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAACggg             >  1:7301870/1‑149 (MQ=255)

                                                                                                                                    aaGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAACggg             >  1:9800365/1‑149 (MQ=255)

                                                                                                                                    aaGATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAACggg             <  1:3471570/149‑1 (MQ=255)

                                                                                                                                      gATTAGAAATTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAACGGGAc           >  2:1655842/1‑149 (MQ=255)

                                                                                                                                       aTTATAAATTACTAAAGAAAGTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAACGGGACa          <  1:71310/149‑1 (MQ=255)

                                                                                                                                            aaaTTACTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAACGGGACAAAGga     <  1:10118135/149‑1 (MQ=255)

ACCATCGATTGCAGCAGACAGTGCTTGGCTTACTAGTTGTTTTTCGTCCATTCACTAATTATACAGAAAAAACACTAATTTTGTCAATATATAGTCCATTATATGGTATAATTATGCTATGAAAAGAGATAAAAGATTAGAAATTATTAAAGAAATTGTGACAAATAATAAAATATTGACTCAAGAAGAGCTTCAATCTCTATTATTAGAACGCGGTGTAGAGGTAACACAAGCCACTTTATCCAGAGATATTAGAAAATTAAATATTATAAAAAAACGGGACAAAGGAGAG  >  AM406671/2266578‑2266869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`