Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 1,933,214 2 bp→CG coding (224‑225/714 nt) atpB ← ATP synthase F0, A subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM4066711,933,2140GC100.0% 4170.8 / NA 1004G75A (GGC→GCG) atpBATP synthase F0, A subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (504/500);  total (504/500)
*AM4066711,933,2150CG99.9% 4124.3 / NA 1004G75A (GGC→GCG) atpBATP synthase F0, A subunit
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base G (504/497);  minor base A (0/1);  total (504/500)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1004 total aligned reads displayed.

CAAGAAGTGCAAGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGGCCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGACCGAAAACAA  >  AM406671/1933072‑1933356
                                                                                                                                              ||                                                                                                                                             
  agaagTGCAAGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCg                                                                                                                                        <  1:9983716/149‑1 (MQ=255)
   gaagTGCAAGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCgt                                                                                                                                       >  2:4715061/1‑149 (MQ=255)
    aagTGCAAGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCgtg                                                                                                                                      <  2:2667985/149‑1 (MQ=255)
      gTGCAAGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCgtggt                                                                                                                                    >  2:9775179/1‑149 (MQ=255)
           attttCAAATCGAATGTTGGCTTTGCAGTTGGTGATTTCCAAAGAGAAACAGAACCTGGGGAACTAATATTAGTTACTAACCAAAAGCAGTTGGGAATAAGAAGGAAAGAAATGAGGGTAAAGTTCATGAGCGCAAAGCGTTGTGCTTc                                                                                                                               <  2:7024619/145‑1 (MQ=255)
                cAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAGGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAATGCGTGGTGCTTCATGAg                                                                                                                          >  2:3677911/1‑149 (MQ=255)
                cAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAg                                                                                                                          <  2:8961422/149‑1 (MQ=255)
                  aaTCGACTGTTGGCTTTGCAGTTGGTGATTTCCAAAGAGAAACATCAGCTGGCGTAATAATAATAGTTACTAGCCCAATGCAGTTAGAAATAAGAAGGAAAGAAAAGAGGGTAAAGTGCATGAGCGCAAAGCGTGGTGCTTCATGAggg                                                                                                                        >  2:1214397/1‑149 (MQ=255)
                  aaTCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAggg                                                                                                                        <  1:742336/149‑1 (MQ=255)
                  aaTCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGAGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAggg                                                                                                                        >  1:7518220/1‑149 (MQ=255)
                   aTCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGc                                                                                                                       >  1:32164/1‑149 (MQ=255)
                    tCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGcc                                                                                                                      >  2:9498650/1‑149 (MQ=255)
                        cTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACg                                                                                                                  <  2:1019047/149‑1 (MQ=255)
                         tGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACgg                                                                                                                 >  2:1576593/1‑148 (MQ=255)
                         tGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGt                                                                                                                 >  1:5027776/1‑149 (MQ=255)
                            tGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTAt                                                                                                              <  1:1264766/149‑1 (MQ=255)
                              gATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCt                                                                                                            <  1:8476027/149‑1 (MQ=255)
                               aTTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTGCATGAGGGCCGACGTTATCtt                                                                                                           >  2:7868195/1‑149 (MQ=255)
                                 ttGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTg                                                                                                         <  1:9469081/149‑1 (MQ=255)
                                      gTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAAc                                                                                                    <  2:2769430/149‑1 (MQ=255)
                                       ttcgtcaTTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACt                                                                                                   <  1:2782602/143‑1 (MQ=255)
                                          gTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCa                                                                                                <  2:4806835/149‑1 (MQ=255)
                                           tGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCAt                                                                                               <  1:3454912/149‑1 (MQ=255)
                                            gATTTCCAAAGAGGAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCAtt                                                                                              >  1:1067785/1‑149 (MQ=255)
                                              tttCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGa                                                                                            >  2:2207643/1‑149 (MQ=255)
                                               ttCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGAc                                                                                           >  1:10309091/1‑149 (MQ=255)
                                                tCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACa                                                                                          >  1:8064747/1‑149 (MQ=255)
                                                 ccAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACaa                                                                                         <  1:5745363/149‑1 (MQ=255)
                                                     agagAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATc                                                                                     <  1:7096227/149‑1 (MQ=255)
                                                     agagAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATc                                                                                     <  1:8657542/149‑1 (MQ=255)
                                                      gagaAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATca                                                                                    <  1:1769893/149‑1 (MQ=255)
                                                       agaAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCACTAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATaaa                                                                                   >  2:5872633/1‑149 (MQ=255)
                                                          aaCATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATcaacaa                                                                                <  2:2430856/149‑1 (MQ=255)
                                                                aCCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACt                                                                          >  1:1620496/1‑149 (MQ=255)
                                                                   tagCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGACCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCaa                                                                       >  1:923819/3‑149 (MQ=255)
                                                                        tACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACa                                                                  >  2:3231028/1‑149 (MQ=255)
                                                                        tACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACa                                                                  <  1:8973888/149‑1 (MQ=255)
                                                                         aCTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACAt                                                                 >  1:5162115/1‑149 (MQ=255)
                                                                          cTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACAtt                                                                >  2:10371385/1‑149 (MQ=255)
                                                                          cTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACAtt                                                                <  1:6176336/149‑1 (MQ=255)
                                                                            aaTTTTAGTTACTAGCCCAATGCAGTTGGCAACAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCCAAGCGTGGTGCCTCATGAGCGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACAtttt                                                              <  1:4140569/149‑1 (MQ=255)
                                                                             aTTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTg                                                             <  2:4985593/149‑1 (MQ=255)
                                                                             aTTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGGAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTg                                                             >  2:1385828/1‑149 (MQ=255)
                                                                              ttttAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGt                                                            <  1:2163320/149‑1 (MQ=255)
                                                                              ttttAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGt                                                            >  1:6866497/1‑149 (MQ=255)
                                                                               ttttGTTACTAGCCCAATGCAGTTAGCAATAAGAAGGAAAGAAAAGAGTGTAAAGTTCATGCGCGCAAAGCGTGGCGCTTCATGAGGGCCGACGTTATCTTTGGACGCTCCATTGACAAAATCAACCACCCACTCAAGAACATTCTGtt                                                           <  2:735863/149‑1 (MQ=255)
                                                                                ttAGTTACTAGCCCAATGCAGTTGGCAATAAGCAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGttt                                                          >  1:7886714/1‑149 (MQ=255)
                                                                                ttAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACAttttatgt                                                          >  2:4134370/1‑145 (MQ=255)
                                                                                ttAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGttt                                                          <  2:6814894/149‑1 (MQ=255)
                                                                                 tAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTa                                                         <  1:9645688/149‑1 (MQ=255)
                                                                                  aGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTAc                                                        >  2:8139008/1‑149 (MQ=255)
                                                                                  aGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTAc                                                        <  1:2593552/149‑1 (MQ=255)
                                                                                   gTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTAcc                                                       >  2:856758/1‑149 (MQ=255)
                                                                                    ttACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAATCTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCt                                                      <  2:7625818/149‑1 (MQ=255)
                                                                                    ttACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCt                                                      <  2:6015019/149‑1 (MQ=255)
                                                                                     tACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAAGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCtt                                                     <  2:6594680/149‑1 (MQ=255)
                                                                                      aCTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCttt                                                    >  1:10034778/1‑149 (MQ=255)
                                                                                      aCTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCttt                                                    <  1:7245872/149‑1 (MQ=255)
                                                                                      aCTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCttt                                                    <  1:4642705/149‑1 (MQ=255)
                                                                                       cTAGCCCAATGCAGTTGGCAAGAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTg                                                   <  1:3059031/149‑1 (MQ=255)
                                                                                         aGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTggg                                                 >  2:840625/1‑149 (MQ=255)
                                                                                          gCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGc                                                <  1:5896318/149‑1 (MQ=255)
                                                                                          gCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCACGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCGATTGACAAAATCAACAACCCACTGATGAACATTTTGTTTACCTTTGGGc                                                <  2:5707966/149‑1 (MQ=255)
                                                                                           cccAATGCAGTTGGCAATAAGAAGGAAAGCAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCt                                               <  2:6229449/149‑1 (MQ=255)
                                                                                           cccAATGCAGTTGGCAATAAGAAGGAAAGACAAGAGGGTAAAGTTCATGAGCGCAAAGCGCGGTGCATCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTCTACCTTTGGGCt                                               >  2:202284/1‑149 (MQ=255)
                                                                                            ccAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCtt                                              >  1:4405727/1‑149 (MQ=255)
                                                                                              aaTGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGa                                            >  1:8292281/1‑149 (MQ=255)
                                                                                               aTGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAg                                           >  1:3605287/1‑149 (MQ=255)
                                                                                                     ttGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCat                                     >  2:1862864/1‑149 (MQ=255)
                                                                                                     ttGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCat                                     <  1:144533/149‑1 (MQ=255)
                                                                                                      tGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCata                                    <  1:5293746/149‑1 (MQ=255)
                                                                                                      tGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCata                                    <  1:648440/149‑1 (MQ=255)
                                                                                                      tGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTCTGGGCTTGAGCTGCata                                    <  2:5375406/149‑1 (MQ=255)
                                                                                                      tGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATCAGGTCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCata                                    <  2:9300791/149‑1 (MQ=255)
                                                                                                        gCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATAtt                                  <  2:4316870/149‑1 (MQ=255)
                                                                                                        gCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATAtt                                  >  2:3429184/1‑149 (MQ=255)
                                                                                                         cAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTg                                 >  1:8780746/1‑149 (MQ=255)
                                                                                                          aaTAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGc                                <  1:4832132/149‑1 (MQ=255)
                                                                                                            tAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGAAGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCgg                              >  1:6275670/1‑149 (MQ=255)
                                                                                                              agaagGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCt                            <  2:2920129/149‑1 (MQ=255)
                                                                                                                aagGAAAGAAAAGAGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCg                          >  1:10120472/1‑149 (MQ=255)
                                                                                                                     aagaaaagaGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAg                     <  1:5601195/149‑1 (MQ=255)
                                                                                                                       gaaaagaGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGaa                   <  1:2415288/149‑1 (MQ=255)
                                                                                                                       gaaaagaGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGaa                   <  1:10415995/149‑1 (MQ=255)
                                                                                                                         aaagaGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATa                 >  2:4479242/1‑149 (MQ=255)
                                                                                                                          aagaGGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATAc                >  2:3825313/1‑149 (MQ=255)
                                                                                                                            gagGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCa              >  2:5577209/1‑149 (MQ=255)
                                                                                                                            gagGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCa              <  2:8272626/149‑1 (MQ=255)
                                                                                                                             agGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCTGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGTATATTGCGGCTCGCCCAGAATACCAg             >  1:6715387/1‑149 (MQ=255)
                                                                                                                             agGGTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAg             >  1:2002311/1‑149 (MQ=255)
                                                                                                                              gggTAACGTTCATGAGCGCAGAGCGTGGTGCGTCGTGGTGGCCGACGTTATCTTTGGAAACCCCAGTGACAAAATCGACAACCCACACAAGAACATTTTGTTTACCTTTGGGCTTGAGGTGCGTATTGCGGCTCGCCCAGAATACCAGa            <  2:519152/149‑1 (MQ=255)
                                                                                                                              gggTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGa            <  2:8670538/149‑1 (MQ=255)
                                                                                                                              gggTAAAGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGa            <  1:3873418/149‑1 (MQ=255)
                                                                                                                                  aaaGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGACCGa        <  1:2351095/149‑1 (MQ=255)
                                                                                                                                    aGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGACCGaaa      >  2:7200064/1‑149 (MQ=255)
                                                                                                                                    aGTTCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATACTGCGGCTCGCCCAGAATACCAGACCGaaa      <  2:6452687/149‑1 (MQ=255)
                                                                                                                                      ttCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGACCGAAAAc    >  2:886345/1‑149 (MQ=255)
                                                                                                                                      ttCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGACCGAAAAc    >  1:9795393/1‑149 (MQ=255)
                                                                                                                                       tCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGACCGAAAACa   <  1:9103669/149‑1 (MQ=255)
                                                                                                                                       tCATGAGCGCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCAACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGACCGAAAACa   >  1:6398229/1‑149 (MQ=255)
                                                                                                                                              ||                                                                                                                                             
CAAGAAGTGCAAGTGTCAAATCGACTGTTGGATTTGCAGTTGGTGATTTCCAAAGAGAAACATCACCTGGCGTACTAATTTTAGTTACTAGCCCAATGCAGTTGGCAATAAGAAGGAAAGAAAAGAGGGTAAAGTTCATGAGGCCAAAGCGTGGTGCTTCATGAGGGCCGACGTTATCTTTGGAAACTCCATTGACAAAATCAACAACCCACTCAAGAACATTTTGTTTACCTTTGGGCTTGAGCTGCATATTGCGGCTCGCCCAGAATACCAGACCGAAAACAA  >  AM406671/1933072‑1933356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑