BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	1,916,379	Δ1 bp	pseudogene (355/598 nt)	llmg_pseudo_59 ←	pseudogene

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	1,916,379	0	T	.	99.4%	4575.0 / 11.3	962	pseudogene (355/598 nt)	llmg_pseudo_59	pseudogene
Reads supporting (aligned to +/- strand): ref base T (5/1); new base . (483/473); total (488/474)
Fisher's exact test for biased strand distribution p-value = 2.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 975 total aligned reads displayed.

CGCGGCTTCAATATCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATATGGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCATTTTTAGATG  >  AM406671/1916235‑1916522

 gcgGCTTCAATATCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑Ggaaga                                                                                                                                           <  2:7816964/149‑1 (MQ=255)

     cTTCAATATCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCATGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAAtt                                                                                                                                       <  2:2942872/149‑1 (MQ=255)

     cTTCAATATCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAAtt                                                                                                                                       <  1:7325609/149‑1 (MQ=255)

      ttCAATATCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTc                                                                                                                                      <  2:5572931/149‑1 (MQ=255)

       tCAATATCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTcc                                                                                                                                     >  2:7325095/1‑149 (MQ=255)

          atatCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTCTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACGTCCAAAAAATA‑GGAAGAAATTCCAAg                                                                                                                                  >  1:6172677/1‑149 (MQ=255)

              ccAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAgttgt                                                                                                                              <  1:3988597/149‑1 (MQ=255)

               cAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAgttgtt                                                                                                                             <  1:7665302/149‑1 (MQ=255)

                    ttCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGt                                                                                                                        >  2:4608617/1‑149 (MQ=255)

                    ttCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGt                                                                                                                        >  2:8528862/1‑149 (MQ=255)

                     tCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTa                                                                                                                       <  1:2743270/149‑1 (MQ=255)

                           aCCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCaa                                                                                                                 >  1:4655432/1‑149 (MQ=255)

                                tataGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAAAAAAAAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCa                                                                                                            <  2:6634501/149‑1 (MQ=255)

                                 ataGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCAt                                                                                                           >  2:7468294/1‑149 (MQ=255)

                                  tagtagGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCAtt                                                                                                          >  2:7617247/1‑149 (MQ=255)

                                        gTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGcc                                                                                                    <  2:59407/149‑1 (MQ=255)

                                        gTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGcc                                                                                                    <  2:58059/149‑1 (MQ=255)

                                         tttCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCt                                                                                                   <  2:1525070/149‑1 (MQ=255)

                                         tttCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCt                                                                                                   <  1:1501155/149‑1 (MQ=255)

                                            catcaGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTtg                                                                                                <  2:1219231/149‑1 (MQ=255)

                                             atcaGTCTTTAATTTAGATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTtgt                                                                                               <  2:1042514/149‑1 (MQ=255)

                                              tcaGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTc                                                                                                                                      >  1:104057/1‑109 (MQ=255)

                                                  tCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGta                                                                                          >  1:5169410/1‑149 (MQ=255)

                                                  tCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGta                                                                                          >  2:5013615/1‑149 (MQ=255)

                                                    tttAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGtata                                                                                        >  1:9442457/1‑149 (MQ=255)

                                                     ttAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATaa                                                                                       >  1:1694529/1‑149 (MQ=255)

                                                      tAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATaaa                                                                                      >  1:3168132/1‑149 (MQ=255)

                                                       aaaTTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTTTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAAt                                                                                     >  1:4957271/1‑149 (MQ=255)

                                                       aaaTTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAAt                                                                                     >  2:3396756/1‑149 (MQ=255)

                                                       aaaTTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAAt                                                                                     <  2:236988/149‑1 (MQ=255)

                                                       aaaTTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAAt                                                                                     >  1:3538127/1‑149 (MQ=255)

                                                        aaTTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATg                                                                                    >  2:5704583/1‑149 (MQ=255)

                                                         aTTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGa                                                                                   <  1:432860/149‑1 (MQ=255)

                                                          ttATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGaa                                                                                  >  2:4391846/1‑149 (MQ=255)

                                                           tataTTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAg                                                                                 <  1:914818/149‑1 (MQ=255)

                                                                tttttAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTg                                                                            <  2:7126826/149‑1 (MQ=255)

                                                                tttttAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTg                                                                            <  1:9354034/149‑1 (MQ=255)

                                                                  tttAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGaa                                                                          >  2:2532063/1‑149 (MQ=255)

                                                                  tttAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGaa                                                                          <  1:1133057/149‑1 (MQ=255)

                                                                    tAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTGTTAAAGTTATTTTCAGAGGTAACAACAAACAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATg                                                                        <  2:3904238/149‑1 (MQ=255)

                                                                     aaCTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCGAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGt                                                                       >  1:2612755/1‑149 (MQ=255)

                                                                     aaCTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGt                                                                       >  1:7817478/1‑149 (MQ=255)

                                                                     aaCTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGt                                                                       >  2:3705612/1‑149 (MQ=255)

                                                                     aaCTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGt                                                                       <  2:6172677/149‑1 (MQ=255)

                                                                       cTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGttt                                                                     >  1:8643651/1‑149 (MQ=255)

                                                                         aaGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTc                                                                   >  2:937620/1‑149 (MQ=255)

                                                                            tAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCttt                                                                <  1:2257455/149‑1 (MQ=255)

                                                                             aaTTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCtttt                                                               >  1:3673219/1‑149 (MQ=255)

                                                                              attgattgCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTTAGTATAAATGAAGTTTTGAATGTTTTCttttt                                                              >  2:1793907/1‑149 (MQ=255)

                                                                                tgattgCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGa                                                            >  2:4089581/1‑149 (MQ=255)

                                                                                tgattgCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGa                                                            >  2:2229756/1‑149 (MQ=255)

                                                                                tgattgCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGa                                                            >  2:677550/1‑149 (MQ=255)

                                                                                tgattgCCACAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGa                                                            >  2:1920300/1‑149 (MQ=255)

                                                                                 gattgCCTCAGGATTCGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAg                                                           <  2:6411813/149‑1 (MQ=255)

                                                                                   ttgCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGCTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGtc                                                         <  1:6025980/149‑1 (MQ=255)

                                                                                    tgCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTTCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGCGtca                                                        >  2:5114560/1‑148 (MQ=255)

                                                                                    tgCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGcc                                                                                                    >  1:4789492/1‑105 (MQ=255)

                                                                                         cAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGtccaatcc                                                   >  1:385567/1‑149 (MQ=255)

                                                                                            gATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCaaa                                                >  1:8331211/1‑149 (MQ=255)

                                                                                             aTTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCaaaa                                               >  1:5972944/1‑149 (MQ=255)

                                                                                              tttGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAAc                                              >  1:6696318/1‑149 (MQ=255)

                                                                                                tGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAActt                                            >  1:6632814/1‑149 (MQ=255)

                                                                                                  gTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAActtct                                          >  1:5677745/1‑149 (MQ=255)

                                                                                                  gTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAActtct                                          >  2:8781325/1‑149 (MQ=255)

                                                                                                   ttttCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAActtctt                                         >  1:2043024/1‑149 (MQ=255)

                                                                                                   ttttCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAActtctt                                         >  1:6911520/1‑149 (MQ=255)

                                                                                                    tttCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCtt                                                                 <  2:5315542/124‑1 (MQ=255)

                                                                                                    tttCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAActtc                                           <  1:8860838/146‑1 (MQ=255)

                                                                                                     ttCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTa                                       >  1:7071795/1‑149 (MQ=255)

                                                                                                      tCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAg                                      <  1:3408591/149‑1 (MQ=255)

                                                                                                      tCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAg                                      >  1:1823106/1‑149 (MQ=255)

                                                                                                      tCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAg                                      <  1:789074/149‑1 (MQ=255)

                                                                                                       cTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTTCAATCCAAAACTTCTTTAGc                                     >  2:8347682/1‑149 (MQ=255)

                                                                                                         tttAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCAc                                   >  1:2442263/1‑149 (MQ=255)

                                                                                                         tttAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCAc                                   >  1:4452147/1‑149 (MQ=255)

                                                                                                         tttAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCAc                                   <  2:7942894/149‑1 (MQ=255)

                                                                                                              aaTTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTAcc                              >  2:3280074/1‑149 (MQ=255)

                                                                                                              aaTTATTTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTAcc                              >  1:1565631/1‑149 (MQ=255)

                                                                                                               aatattTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTTTTCGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTTAATGTTTTCTTTTTGAGTCTAATACAAAACTTCTTTAGCATCTActa                             >  2:6974179/3‑147 (MQ=255)

                                                                                                                ttattTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCAt                            <  2:2083581/149‑1 (MQ=255)

                                                                                                                  attTTCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTa                          >  1:9228236/1‑149 (MQ=255)

                                                                                                                   ttttCAGAGGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTaa                         <  2:3596184/149‑1 (MQ=255)

                                                                                                                        agagGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAatttta                    >  2:8226772/1‑149 (MQ=255)

                                                                                                                        agagGTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAatttta                    >  1:6401958/1‑149 (MQ=255)

                                                                                                                            gTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAattttatttt                <  1:7461564/149‑1 (MQ=255)

                                                                                                                            gTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAattttatttt                <  1:8165327/149‑1 (MQ=255)

                                                                                                                            gTAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAattttatttt                <  2:1360185/149‑1 (MQ=255)

                                                                                                                             tAACAACTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTg               >  2:4866467/1‑149 (MQ=255)

                                                                                                                              aacaacTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTTTTTTTTTTTTTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGt              >  2:9437151/1‑149 (MQ=255)

                                                                                                                              aacaacTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGt              >  2:3076927/1‑149 (MQ=255)

                                                                                                                                caacTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCa            >  2:9167134/1‑149 (MQ=255)

                                                                                                                                  acTTCCAAAAAATA‑GGAAGAAATTCCGAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTANCACCTACCATTAATTTTATTTTGTCAtt          <  1:5367695/149‑1 (MQ=255)

                                                                                                                                  acTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCAtt          <  1:4134127/149‑1 (MQ=255)

                                                                                                                                   cTTCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCAttt         >  2:2783451/1‑149 (MQ=255)

                                                                                                                                    ttCCAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCAtttt        <  2:8936323/149‑1 (MQ=255)

                                                                                                                                     accAAAATATAT‑GAAGAAATTCAAAGTTGTTCTAGTAAATCAAATGTATTTTTGCGTTTGTGAGTATAAATTATGTTTTGAATGTTTTATTTATGAATCTACTTCAAACCTTCTTTAGCACCTACCATTATTTTTATTTTGTCAttttt       <  2:4712205/148‑1 (MQ=255)

                                                                                                                                      ccAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCATTTTTa      >  1:2923843/1‑149 (MQ=255)

                                                                                                                                      ccAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCATTTTTa      >  1:1233764/1‑149 (MQ=255)

                                                                                                                                      ccAAAAAATA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCATTTTTa      <  1:8041840/149‑1 (MQ=255)

                                                                                                                                        aaaaaaTA‑GGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCATTTTTAGa    <  2:6841599/149‑1 (MQ=255)

CGCGGCTTCAATATCCAATTTTCGGTCACCGATATAGTAGGTTTCATCAGTCTTTAAATTATATTTTTTAACTAAGTAATTGATTGCCTCAGGATTTGGTTTTCTTTTAAAATTATTTTCAGAGGTAACAACTTCCAAAAAATATGGAAGAAATTCCAAGTTGTTTGAGTAAATCAAAGGCATTTTTGCCTTTGTGAGTATAAATGAAGTTTTGAATGTTTTCTTTTTGAGTCCAATCCAAAACTTCTTTAGCACCTACCATTAATTTTATTTTGTCATTTTTAGATG  >  AM406671/1916235‑1916522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`