BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	1,921,639	Δ1 bp	intergenic (‑129/‑256)	llmg_1941 ← / → glnP	conserved hypothetical protein/glutamine ABC transporter permease and substrate binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	1,921,636	0	T	.	99.8%	4817.4 / ‑0.2	1005	intergenic (‑126/‑259)	llmg_1941/glnP	conserved hypothetical protein/glutamine ABC transporter permease and substrate binding protein
Reads supporting (aligned to +/- strand): ref base T (0/1); major base . (498/504); minor base C (0/2); total (498/507)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 1063 total aligned reads displayed.

AAAAATCGAAAGTTGTACAAACATAATATCCCTTTTCAAAAGTTTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAACTTTTCTTATATTTTATTCAGGAGAACTCTTCAAATTTCAACAAATTTCAAAATGTCAGGAAAACCTAAACATTCAACTTGATTTCTAGAACAATTTTTGTGATATAATTTTTCCCAGAAATAAAATAAACTGAGCTTGTCTTCCTAAATATAGGGAAA  >  AM406671/1921487‑1921793

aaaaaTCGAAAGTTGTACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAACt                                                                                                                                                               >  2:105386/1‑149 (MQ=255)

  aaaTCGAAAGTTGTACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAACttt                                                                                                                                                             >  1:7026884/1‑149 (MQ=255)

  aaaTCGAAAGTTGTACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAACttt                                                                                                                                                             <  2:1648411/149‑1 (MQ=255)

   aaacGAAAGATGTAAAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAAAAAAAAAAAAAAAAGGACTTTTTGACAATATAAACtttc                                                                                                                                                            <  2:8191613/146‑2 (MQ=255)

   aaTCGAAAGTTGTACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAACtttc                                                                                                                                                            <  1:1744729/149‑2 (MQ=255)

   aaTCGAAAGTTGTACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAACtttc                                                                                                                                                            >  2:2282233/1‑148 (MQ=255)

      cGAGAGTTGTACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAACtttctta                                                                                                                                                         <  2:1253689/149‑5 (MQ=255)

      cGAAAGTTGTACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAACtttctta                                                                                                                                                         >  1:2568214/1‑145 (MQ=255)

       gAAAGTTGTACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTtat                                                                                                                                                       <  1:8133472/149‑1 (MQ=255)

       gAAAGTTGTACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTCACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTtat                                                                                                                                                       <  2:7372709/149‑1 (MQ=255)

       gAAAGTTGTACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACACGTTCAATTTTACAACAAAATGGACTTTTTGACAATATAAAC‑TTTCTtat                                                                                                                                                       <  2:5955013/149‑1 (MQ=255)

       gAAAATTGTACAAACATAATATAACTTTTCAAAA‑ATTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGCATACATAAATATCTCGATCAAGCACTCGTTCAAATTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTtat                                                                                                                                                       <  1:7628818/149‑1 (MQ=255)

        aaaGTTGTACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAACgttcgtata                                                                                                                                                       >  2:6116213/1‑143 (MQ=255)

        aaaGTTGTACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTtata                                                                                                                                                      >  2:8609650/1‑149 (MQ=255)

         aaGTTGTACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTatat                                                                                                                                                     >  1:7781525/1‑149 (MQ=255)

            ttGTACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATAtttt                                                                                                                                                  <  1:8818450/149‑1 (MQ=255)

               tACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTAtc                                                                                                                                               >  2:3395656/1‑148 (MQ=255)

               tACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTAtc                                                                                                                                               >  2:4068560/1‑148 (MQ=255)

               tACAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTAtc                                                                                                                                               <  1:4993496/149‑2 (MQ=255)

                 cAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTAtcag                                                                                                                                             <  1:680532/149‑4 (MQ=255)

                 cAAACATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAAGATCTCTATCAATCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTAtcag                                                                                                                                             >  2:5352100/1‑146 (MQ=255)

                   aaCATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTAtcagag                                                                                                                                           >  1:3260802/1‑144 (MQ=255)

                   aaCATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTAtcagag                                                                                                                                           >  2:2482239/1‑144 (MQ=255)

                     cATAATATTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTAtcagagaa                                                                                                                                         >  1:1911068/1‑142 (MQ=255)

                        aataTTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAActc                                                                                                                                    >  1:128513/1‑149 (MQ=255)

                        aataTTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAActc                                                                                                                                    >  1:7993878/1‑149 (MQ=255)

                         atatTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTAAAAAAAAAAAAAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAActct                                                                                                                                   <  2:1725073/149‑1 (MQ=255)

                          tatTCCTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAActctc                                                                                                                                  <  2:8370685/149‑2 (MQ=255)

                            ttccTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAActctcaa                                                                                                                                >  2:7221287/3‑146 (MQ=255)

                              ccTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑Tcaaat                                                                                                                             >  1:8299453/1‑149 (MQ=255)

                               cTTTTCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑Tcaaatt                                                                                                                            >  1:7505476/1‑149 (MQ=255)

                                 tttCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑Tcaaatttc                                                                                                                          >  2:424890/1‑149 (MQ=255)

                                 tttCAAAAG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑Tcaaatttc                                                                                                                          <  1:27409/149‑1 (MQ=255)

                                     aaaaG‑TTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑Tcaaatttcacaa                                                                                                                      <  1:3398607/149‑4 (MQ=255)

                                        aagttAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑tcaaatttc‑acaaat                                                                                                                   <  1:6457238/146‑1 (MQ=255)

                                         agttAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑tcaaatttc‑acaaatt                                                                                                                  <  1:5148240/147‑1 (MQ=255)

                                           ttAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑tcaaatttc‑acaaattca                                                                                                                >  2:1932846/1‑147 (MQ=255)

                                            tAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑tcaaatttc‑acaaattcaa                                                                                                               >  2:4904757/1‑146 (MQ=255)

                                            tAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑CCA‑GCGAACTC‑tcaaatttc‑acaaattcaa                                                                                                               >  2:5713518/1‑146 (MQ=255)

                                              aaaaTTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCaaaa                                                                                                            <  1:424890/149‑1 (MQ=255)

                                              aaaaTTCTTACAGTAGTTCTGCTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCaaaa                                                                                                            <  2:7706269/149‑1 (MQ=255)

                                                aaTTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATg                                                                                                          >  2:6657729/1‑149 (MQ=255)

                                                aaTTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATg                                                                                                          <  2:2080825/149‑1 (MQ=255)

                                                 aTTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGt                                                                                                         >  2:1360778/1‑149 (MQ=255)

                                                  ttCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTc                                                                                                        >  2:9437654/1‑149 (MQ=255)

                                                    cTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCAg                                                                                                      <  1:9312682/149‑1 (MQ=255)

                                                     ttACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCAga                                                                                                     >  1:393932/1‑148 (MQ=255)

                                                      tACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCAgaa                                                                                                    <  1:4829265/149‑3 (MQ=255)

                                                      tACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCAgaa                                                                                                    >  2:2597631/1‑147 (MQ=255)

                                                        cAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCAGaaaa                                                                                                  >  1:2832428/1‑149 (MQ=255)

                                                        cAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCAGaaaa                                                                                                  <  1:382530/149‑1 (MQ=255)

                                                         cgtagtTCTGTTCACTTGCATCGAATACATAAATATCCCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGACCTC‑TCAAATTTC‑ACAAA‑TTGAAAATGTCAgaatac                                                                                                 >  2:5515415/2‑144 (MQ=255)

                                                          gtagtTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAAcc                                                                                               >  1:9540633/1‑149 (MQ=255)

                                                           tagtTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCt                                                                                              <  2:7070890/149‑1 (MQ=255)

                                                             gtTCTGTTTACTTACATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAAC‑CCTCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCTaa                                                                                            <  2:3931104/149‑1 (MQ=255)

                                                             gtTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCTaa                                                                                            >  1:5791120/1‑149 (MQ=255)

                                                              ttCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCTaac                                                                                           >  1:5633531/1‑148 (MQ=255)

                                                               tCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCTaaca                                                                                          <  2:5795235/149‑3 (MQ=255)

                                                                 tGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAAcc                                                                                               <  1:2098995/142‑1 (MQ=255)

                                                                   tttACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCTaacatcaa                                                                                      >  1:4090106/1‑143 (MQ=255)

                                                                    ttACTTAAATAGAATACATAAATATCTCTATCAAGCGCTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCTaacatcaac                                                                                     <  2:9229920/149‑8 (MQ=255)

                                                                    ttACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCTaacatcaac                                                                                     <  2:2983027/149‑8 (MQ=255)

                                                                        ttAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGa                                                                               <  2:9080651/149‑1 (MQ=255)

                                                                              aGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGAttctag                                                                         >  1:1085412/1‑145 (MQ=255)

                                                                                aaTACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAGac                                                                      >  1:8675080/1‑148 (MQ=255)

                                                                                 aTACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAGaca                                                                     <  2:7931630/149‑3 (MQ=255)

                                                                                 aTACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAGaca                                                                     <  1:1583813/149‑3 (MQ=255)

                                                                                    cATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TACAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAAtt                                                                 >  2:6282713/1‑149 (MQ=255)

                                                                                     aTAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAAttt                                                                <  1:6276097/149‑1 (MQ=255)

                                                                                         atatCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTtgt                                                            <  1:7363949/149‑1 (MQ=255)

                                                                                           atCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGa                                                          <  2:3682660/149‑1 (MQ=255)

                                                                                            tctcTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGat                                                         <  1:4606167/149‑1 (MQ=255)

                                                                                              tctATCAAGCACTCGTTCAATTTTACGACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTC‑TGAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGatat                                                       <  1:6162387/149‑1 (MQ=255)

                                                                                               ctATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGAtata                                                      <  2:5160879/149‑1 (MQ=255)

                                                                                               ctATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGAtata                                                      <  1:9165252/149‑1 (MQ=255)

                                                                                               ctATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGAtata                                                      >  1:5439567/1‑149 (MQ=255)

                                                                                                     aGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAAttttc                                                <  2:1519164/149‑2 (MQ=255)

                                                                                                     aGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAAttttc                                                <  1:3589586/149‑2 (MQ=255)

                                                                                                     aGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAAttttc                                                >  1:2385068/1‑148 (MQ=255)

                                                                                                         cTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAg                                           <  2:2765797/149‑1 (MQ=255)

                                                                                                           cGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAGaa                                         <  2:6812230/149‑1 (MQ=255)

                                                                                                              tCAATTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAGaataa                                      >  1:1409954/1‑146 (MQ=255)

                                                                                                                 aTTTTACAACAAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAg‑aataaaat                                  <  1:6658899/149‑1 (MQ=255)

                                                                                                                      acaacaAAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACt                             <  1:4447777/149‑1 (MQ=255)

                                                                                                                       caacaaAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTg                            >  2:4471554/1‑149 (MQ=255)

                                                                                                                         acaaAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAg                          <  1:1040756/149‑1 (MQ=255)

                                                                                                                          caaAAAGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATANAA‑TTTTCCCAG‑AATAAAATAAACTGAGc                         <  1:5352100/149‑1 (MQ=255)

                                                                                                                           aaaaaGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCt                        >  2:6538905/1‑149 (MQ=255)

                                                                                                                            aaaaGGACTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCtt                       >  2:3824225/1‑149 (MQ=255)

                                                                                                                                  aCTTTTTGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTCtcc                 >  2:8852616/1‑147 (MQ=255)

                                                                                                                                      tttGACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTaaa            >  1:890464/1‑149 (MQ=255)

                                                                                                                                         gACAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAatat         <  1:8484908/149‑1 (MQ=255)

                                                                                                                                          aCAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAAtata        <  1:7221287/149‑1 (MQ=255)

                                                                                                                                           cAATATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAg       >  2:7560822/1‑149 (MQ=255)

                                                                                                                                            aaTATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAgg      <  1:3045974/149‑1 (MQ=255)

                                                                                                                                            aaTATAAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAgg      >  2:4772993/1‑149 (MQ=255)

                                                                                                                                              tataAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTACATATAggaa    >  2:507246/1‑147 (MQ=255)

                                                                                                                                              tataAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAggaa    >  2:804760/1‑147 (MQ=255)

                                                                                                                                               ataAAC‑TTTCTTATATTTTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAggaaa   >  2:3149727/1‑146 (MQ=255)

                                                                                                                                                taAAC‑TTTCTTATATATTA‑TCA‑GAGAACTC‑TCAAATTTC‑ACAAA‑TTCAAAATGTCA‑GAAAACCT‑AACA‑TCAACTTGA‑TTCTAG‑ACAATTTTTGTGATATAA‑TTTTCCCAG‑AATAAAATAAACTGAGCTTGTC‑TCCTAAATATAGGaaac  <  2:454712/149‑2 (MQ=255)

AAAAATCGAAAGTTGTACAAACATAATATCCCTTTTCAAAAGTTTAAAAATTCTTACAGTAGTTCTGTTTACTTAAATAGAATACATAAATATCTCTATCAAGCACTCGTTCAATTTTACAACAAAAAGGACTTTTTGACAATATAAACTTTTCTTATATTTTATTCAGGAGAACTCTTCAAATTTCAACAAATTTCAAAATGTCAGGAAAACCTAAACATTCAACTTGATTTCTAGAACAATTTTTGTGATATAATTTTTCCCAGAAATAAAATAAACTGAGCTTGTCTTCCTAAATATAGGGAAA  >  AM406671/1921487‑1921793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`