BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	945,239	+C	pseudogene (229/761 nt)	tnp904 →	pseudogene

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	945,239	1	.	C	99.3%	1010.2 / 1.3	441	pseudogene (229/761 nt)	tnp904	pseudogene
Reads supporting (aligned to +/- strand): ref base . (1/2); new base C (212/226); total (213/228)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 480 total aligned reads displayed.

GGGACGCTATGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑G‑TTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATGGCTGGGAGACATGACCTATATCCCTACCAAAGAAGGCACCTTATACTTAGCCGTGAATATCGACGTT  >  AM406671/945091‑945369

 ggACGCTATGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑gc                                                                                                                                    <  1:8448361/149‑2 (MQ=38)

   aCGCTATGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑gctt                                                                                                                                  <  2:1865054/149‑4 (MQ=37)

      cTATGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGc                                                                                                                               <  2:7430682/149‑1 (MQ=21)

      cTATGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGc                                                                                                                               <  2:6838302/149‑1 (MQ=21)

       tATGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCt                                                                                                                              >  1:6336037/1‑149 (MQ=21)

       tATGGTGCGGTTAGAAGTACCAAAGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCt                                                                                                                              <  2:6184587/149‑1 (MQ=255)

        aTGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCtt                                                                                                                             >  1:1527191/1‑149 (MQ=17)

        aTGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCtt                                                                                                                             >  1:7262557/1‑149 (MQ=21)

        aTGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCtt                                                                                                                             >  2:5018906/1‑149 (MQ=17)

        aTGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCtt                                                                                                                             <  1:4701386/149‑1 (MQ=21)

         tGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCttt                                                                                                                            <  2:9391772/149‑1 (MQ=21)

          ggTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTc                                                                                                                           >  1:7561449/1‑149 (MQ=21)

           gTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGATGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCa                                                                                                                          >  1:5742727/1‑149 (MQ=255)

             gCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAg                                                                                                                        <  1:8739678/149‑1 (MQ=21)

                gTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAGCCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGAcc                                                                                                                     <  1:6914275/149‑1 (MQ=16)

                gTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAAAACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGAcc                                                                                                                     <  2:3391444/149‑1 (MQ=12)

                 ttAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGAccc                                                                                                                    >  2:8010615/1‑149 (MQ=18)

                  tAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCa                                                                                                                   >  1:6188159/1‑149 (MQ=21)

                     aaTTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAAtt                                                                                                                >  2:8438890/1‑149 (MQ=17)

                     aaTTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAAtt                                                                                                                >  2:2572625/1‑149 (MQ=21)

                      aTTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAAttt                                                                                                               <  1:9089808/149‑1 (MQ=18)

                        tACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATttaa                                                                                                             <  2:9014707/149‑1 (MQ=21)

                         aCCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATttaat                                                                                                            <  2:659975/149‑1 (MQ=18)

                          ccAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATttaatt                                                                                                           >  2:882560/1‑149 (MQ=17)

                          ccAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATttaatt                                                                                                           <  2:2359872/149‑1 (MQ=17)

                           cAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATttaatta                                                                                                          <  1:784256/149‑1 (MQ=21)

                           cAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATttaatta                                                                                                          >  1:379531/1‑149 (MQ=21)

                            aaGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCGTCGCTTTCAAGACCCAATttaattaa                                                                                                         <  2:448709/149‑1 (MQ=12)

                             aGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTtaattaat                                                                                                        >  1:4071934/1‑149 (MQ=17)

                              ggTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATGTAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATc                                                                                                       <  2:6973021/149‑1 (MQ=16)

                              ggTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATc                                                                                                       <  2:5180081/149‑1 (MQ=21)

                              ggTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATc                                                                                                       <  1:7405851/149‑1 (MQ=18)

                                tACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAg                                                                                                     <  2:3055646/149‑1 (MQ=21)

                                tACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAg                                                                                                     <  2:1547342/149‑1 (MQ=17)

                                tACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAg                                                                                                     >  2:1130156/1‑149 (MQ=21)

                                tACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAg                                                                                                     <  1:325477/149‑1 (MQ=17)

                                tACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAg                                                                                                     <  1:2910985/149‑1 (MQ=21)

                                tACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAg                                                                                                     >  1:3078649/1‑149 (MQ=21)

                                tACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTAAATTAATCAg                                                                                                     >  1:131339/1‑149 (MQ=12)

                                 aCTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGa                                                                                                    >  1:1220225/1‑149 (MQ=21)

                                 aCTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGa                                                                                                    >  1:3691657/1‑149 (MQ=21)

                                 aCTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGa                                                                                                    <  2:5836635/149‑1 (MQ=21)

                                   ttCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATc                                                                                                  >  1:5429129/1‑149 (MQ=21)

                                   ttCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATc                                                                                                  >  2:571664/1‑149 (MQ=21)

                                   ttCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATc                                                                                                  <  1:9294309/149‑1 (MQ=18)

                                    tCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATcc                                                                                                 >  2:7225181/1‑148 (MQ=17)

                                     cATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCtt                                                                                                <  2:4341749/149‑1 (MQ=21)

                                      ataataCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCttt                                                                                               <  2:2157206/149‑1 (MQ=17)

                                       taataCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTa                                                                                              >  1:9399849/1‑149 (MQ=21)

                                       taataCTGGTATTATAACCAACACGAAACGTGTGAGAAAACTGATGCACTTGATGAGACTTTATACAAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTa                                                                                              <  2:3446067/149‑1 (MQ=11)

                                        aataCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTaa                                                                                             >  2:778584/1‑149 (MQ=17)

                                        aataCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTaa                                                                                             <  1:5617242/149‑1 (MQ=21)

                                        aataCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTaa                                                                                             <  1:7737638/149‑1 (MQ=21)

                                        aataCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCGTTCAAGACCCAATTTAATTAATCAGATCTTTaa                                                                                             >  1:1103094/1‑149 (MQ=12)

                                          taCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAg                                                                                           <  1:7652913/149‑1 (MQ=21)

                                           aCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCGCATGATGGGACGTTATGCCAGGGGGCGCCGTTATAAAAATAAAAATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGc                                                                                          >  2:6391430/1‑149 (MQ=11)

                                           aCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCCAGGGAAGCCGTTATAAATATCAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATCTAATTAATCAGATCTTTAAAGc                                                                                          >  1:6527859/1‑149 (MQ=11)

                                           aCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGc                                                                                          <  2:1382486/149‑1 (MQ=21)

                                           aCTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGc                                                                                          >  1:5108489/1‑149 (MQ=21)

                                           aCTGGTATTATAACCAACACGAAACGTGTGGGGAAACAGAGGCAGATGCTGGGTAGATATGCGAGGGGGAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAAACCCAATTTAATTAATCAGATCTTTAAAGc                                                                                          >  2:4526367/1‑149 (MQ=11)

                                            cTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCa                                                                                         >  2:8290832/1‑149 (MQ=21)

                                             tGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCaa                                                                                        <  2:4098521/149‑1 (MQ=17)

                                                tattatAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAg                                                                                     >  2:7300663/1‑149 (MQ=18)

                                                tattatAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAg                                                                                     <  1:590711/149‑1 (MQ=21)

                                                tattatAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAg                                                                                     >  2:4877728/1‑149 (MQ=21)

                                                tattatAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAg                                                                                     >  1:6711063/1‑149 (MQ=21)

                                                 attatAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGc                                                                                    <  1:9127633/149‑1 (MQ=18)

                                                 attatAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGc                                                                                    >  1:8286524/1‑149 (MQ=21)

                                                  ttatAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCt                                                                                   <  2:5512017/149‑1 (MQ=17)

                                                  ttatAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCt                                                                                   <  2:6661385/149‑1 (MQ=17)

                                                   tataACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTCTAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTc                                                                                  <  2:6099856/149‑1 (MQ=16)

                                                   tataACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTc                                                                                  >  1:9605217/1‑149 (MQ=21)

                                                   tataACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTc                                                                                  <  2:191797/149‑1 (MQ=17)

                                                     taACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCt                                                                                <  1:1862616/149‑1 (MQ=17)

                                                      aaCCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCta                                                                               <  1:229819/149‑1 (MQ=14)

                                                      aaCCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCta                                                                               <  2:2889561/149‑1 (MQ=14)

                                                        ccAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCtaat                                                                             >  1:1058879/1‑149 (MQ=11)

                                                          aaCACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCtaataa                                                                           <  1:4942123/149‑1 (MQ=11)

                                                          aaCACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCtaataa                                                                           >  2:8082178/1‑149 (MQ=14)

                                                          aaCACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCtaataa                                                                           >  1:8198167/1‑149 (MQ=14)

                                                           acacGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCACGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATaaa                                                                          >  1:7136289/1‑149 (MQ=2)

                                                           acacGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATaaa                                                                          >  2:3753695/1‑149 (MQ=11)

                                                            cacGAAACGTGTGGGGAAACTGATTCACTTGATGGGACTTTATGCGAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACCGCTCCTAATAAAg                                                                         >  2:2807502/1‑149 (MQ=1)

                                                            cacGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAg                                                                         >  1:4398644/1‑149 (MQ=14)

                                                             acGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGt                                                                        <  1:8086465/149‑1 (MQ=11)

                                                             acGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGt                                                                        >  1:3626645/1‑149 (MQ=14)

                                                              cGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTa                                                                       <  2:3901787/149‑1 (MQ=14)

                                                                aaaCGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATg                                                                     <  2:5562622/149‑1 (MQ=11)

                                                                 aaCGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATgg                                                                    <  1:3391685/149‑1 (MQ=1)

                                                                 aaCGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATgg                                                                    <  1:1805980/149‑1 (MQ=14)

                                                                 aaCGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATgg                                                                    >  2:1724762/1‑149 (MQ=14)

                                                                  aCGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATGGc                                                                   >  1:4194586/1‑149 (MQ=1)

                                                                  aCGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATGGc                                                                   >  2:5924735/1‑149 (MQ=14)

                                                                  aCGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATGGc                                                                   >  1:2220509/1‑149 (MQ=14)

                                                                  aCGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATGGc                                                                   <  2:2698420/149‑1 (MQ=14)

                                                                   cGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACATAAAAGGATC‑TTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATGGCt                                                                  <  2:161863/149‑1 (MQ=2)

                                                                   cGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATGGCt                                                                  <  1:2816144/149‑1 (MQ=14)

                                                                   cGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATGGCt                                                                  <  1:2551063/149‑1 (MQ=11)

                                                                   cGTGTGGAGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACACAATTTAATTAATCAGATCTTTAAAGCAACTGCTCCTAATAAAGTATGGCt                                                                  <  2:6709222/149‑1 (MQ=1)

                                                                      gtgGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑GCTTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATGGCTggg                                                               >  1:4816372/1‑149 (MQ=11)

GGGACGCTATGGTGCGGTTAGAATTACCAAGGTACTTCATAATACTGGTATTATAACCAACACGAAACGTGTGGGGAAACTGATGCACTTGATGGGACTTTATGCCAAGGGAAGCCGTTATAAATATAAACATTATAACAGAAAAGGA‑G‑TTCGCTTTCAAGACCCAATTTAATTAATCAGATCTTTAAAGCAACAGCTCCTAATAAAGTATGGCTGGGAGACATGACCTATATCCCTACCAAAGAAGGCACCTTATACTTAGCCGTGAATATCGACGTT  >  AM406671/945091‑945369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`