Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 1,210,283 (A)5→6 pseudogene (515/1046 nt) llmg_pseudo_39 ← pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM4066711,210,2781.A98.5% 2896.7 / 22.8 736pseudogene (520/1046 nt)llmg_pseudo_39pseudogene
Reads supporting (aligned to +/- strand):  ref base . (8/3);  new base A (377/347);  total (386/350)
Fisher's exact test for biased strand distribution p-value = 2.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 803 total aligned reads displayed.

TTCAGTCAAAATTTCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAG‑A‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTGCATCAA  >  AM406671/1210130‑1210421
                                                                                                                                                     |                                                                                                                                                 
  cAGTCAAAATTTCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAG‑a‑a                                                                                                                                                <  2:6045378/149‑1 (MQ=255)
   aGTCAAAATTTCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAG‑a‑aa                                                                                                                                               >  1:4087045/1‑149 (MQ=255)
      cAAAATTTCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAG‑a‑aaaaa                                                                                                                                            <  1:7545608/149‑2 (MQ=255)
        aaaTTTCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAG‑a‑aaaaata                                                                                                                                          <  1:6527989/149‑4 (MQ=255)
         aaTTTCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAG‑a‑aaaaatat                                                                                                                                         >  1:8352082/1‑145 (MQ=255)
             tctcGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGc                                                                                                                                     <  1:6253180/149‑1 (MQ=255)
             tctcGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGc                                                                                                                                     >  1:8533650/1‑149 (MQ=255)
               tcGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTg                                                                                                                                   >  1:3610908/1‑149 (MQ=255)
               tcGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTg                                                                                                                                   >  1:1737359/1‑149 (MQ=255)
               tcGAAAACTATCTGTTCTCTCCTGAGTCATCAATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTAAAAAAAAA‑AAAA‑TATTTGCTg                                                                                                                                   <  2:2901151/149‑1 (MQ=255)
                  aaaaCTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACa                                                                                                                                >  2:6263537/1‑149 (MQ=255)
                   aaaCTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAg                                                                                                                               >  2:5897823/1‑149 (MQ=255)
                    aaCTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGa                                                                                                                              >  1:4473088/1‑149 (MQ=255)
                      cTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATg                                                                                                                            <  1:699218/149‑1 (MQ=255)
                        aTCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAg                                                                                                                          >  2:7249710/1‑149 (MQ=255)
                         tCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGt                                                                                                                         >  2:5135336/1‑149 (MQ=255)
                          cTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGtt                                                                                                                        <  1:7690670/149‑1 (MQ=255)
                            gTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCTTGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGCGAA‑AAAA‑TATTTGCTCACAGATGAGTTaa                                                                                                                      >  2:8801330/1‑149 (MQ=255)
                            gTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTaa                                                                                                                      >  2:470247/1‑149 (MQ=255)
                                tctcCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCa                                                                                                                  >  1:4858908/1‑149 (MQ=255)
                                 ctcCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCAc                                                                                                                 <  2:3586162/149‑1 (MQ=255)
                                 ctcCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCAc                                                                                                                 >  1:2965519/1‑149 (MQ=255)
                                  tcCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGACTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACg                                                                                                                >  2:4488372/1‑149 (MQ=255)
                                   ccTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGt                                                                                                               <  1:2426076/149‑1 (MQ=255)
                                      gAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCa                                                                                                            >  1:3912061/1‑149 (MQ=255)
                                       aGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCaa                                                                                                           >  1:6764806/1‑149 (MQ=255)
                                       aGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATAACGTCCaa                                                                                                           >  1:5554360/1‑149 (MQ=255)
                                         tCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGt                                                                                                         <  1:7355943/149‑1 (MQ=255)
                                               aTGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATa                                                                                                   <  2:2525428/149‑1 (MQ=255)
                                                tGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATaa                                                                                                  >  2:6162601/1‑149 (MQ=255)
                                                 gTATTGGACGAACCACCAATGAAAGCTAAATTCTGATAGCGTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCGGACAGATGAGTTAAATCACGTCCAAGTAGAATAAg                                                                                                 <  2:3482141/149‑1 (MQ=255)
                                                      ggACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGaa                                                                                            >  1:4250538/1‑149 (MQ=255)
                                                       gACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAAt                                                                                           <  2:4099186/149‑1 (MQ=255)
                                                         cGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAAttt                                                                                         >  1:5427472/1‑149 (MQ=255)
                                                           aaCCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCt                                                                                       <  2:9327164/149‑1 (MQ=255)
                                                            accaccACTGAAAGATAATTTCTGATAGCCTTTTTGAAGCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGGTGGTCTTTTGTGAGGG‑AGAAGC‑TAGTTGCTGACTGATGAGTGAAATCACGTCGAAGGAGAACAAGGGGagcttatt                                                                                      >  2:7744672/1‑142 (MQ=255)
                                                              caccaATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAAAAAGTAGCTGAGTGGGAGTGCGTTGATCTCTTTTTAGAG‑A‑AAAAGTATTTGCTGACAGATTAGTTAAATCACGTACAAGTAGAATAAGCGGAATTTCtttt                                                                                    >  2:3314535/1‑148 (MQ=255)
                                                               accaATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTgg                                                                                   >  1:9022596/1‑149 (MQ=255)
                                                                ccaATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGc                                                                                  >  1:8845210/1‑149 (MQ=255)
                                                                  aaTGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTa                                                                                <  1:8730692/149‑1 (MQ=255)
                                                                   aTGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTaa                                                                               <  1:8253672/149‑1 (MQ=255)
                                                                   aTGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTaa                                                                               <  2:864761/149‑1 (MQ=255)
                                                                    tGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTaaa                                                                              <  1:2254035/149‑1 (MQ=255)
                                                                           tAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTaa                                                                       <  1:5958533/149‑1 (MQ=255)
                                                                            aaTTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAag                                                                      >  2:9184142/1‑149 (MQ=255)
                                                                             aTTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAaga                                                                     <  1:3260930/149‑1 (MQ=255)
                                                                               ttCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGCGTTAAATCACGTCCAAGTAGAATCAGGGGAATTTCTTTGGCTAAATTGCTAagatc                                                                   >  2:6425170/1‑147 (MQ=255)
                                                                                  tGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGagcagc                                                                <  2:5017413/149‑1 (MQ=255)
                                                                                   gATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCt                                                               <  1:2798794/149‑1 (MQ=255)
                                                                                   gATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTGGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCt                                                               <  1:316506/149‑1 (MQ=255)
                                                                                    aTAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCtt                                                              <  2:4592196/149‑1 (MQ=255)
                                                                                      aGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTgg                                                            <  2:1613988/149‑1 (MQ=255)
                                                                                       gCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGt                                                           >  2:1055242/1‑149 (MQ=255)
                                                                                        ccTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTa                                                          >  1:1560414/1‑149 (MQ=255)
                                                                                        ccTTTTTGAATCATGTGAGAAACAAGTAGTTGAGCGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAACAAGGGGAATTTCTTTGGCTAAATTGCCAAGAGCAGCTCGGtc                                                          >  2:1425415/1‑148 (MQ=255)
                                                                                          tttttGAGGCATGTGAGAAACAAGGAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGt                                                        <  2:8186572/149‑1 (MQ=255)
                                                                                          tttttGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGt                                                        <  1:8797544/149‑1 (MQ=255)
                                                                                           ttttGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTc                                                       <  2:3898387/149‑1 (MQ=255)
                                                                                            tttGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCa                                                      <  2:315619/149‑1 (MQ=255)
                                                                                              tGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAgg                                                    >  2:8059215/1‑149 (MQ=255)
                                                                                               gAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGa                                                   <  1:9231038/149‑1 (MQ=255)
                                                                                               gAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGa                                                   >  2:8454071/1‑149 (MQ=255)
                                                                                                aaTCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGAt                                                  <  2:540317/149‑1 (MQ=255)
                                                                                                  tCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTa                                                <  2:2343258/149‑1 (MQ=255)
                                                                                                   cATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTaa                                               >  1:1944815/1‑149 (MQ=255)
                                                                                                    aTGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAat                                              >  1:5724239/1‑149 (MQ=255)
                                                                                                     tgtgAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAata                                             <  1:9562330/149‑1 (MQ=255)
                                                                                                      gtgAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTAAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAatat                                            <  2:4244640/149‑1 (MQ=255)
                                                                                                        gagaAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCa                                          <  1:5097319/149‑1 (MQ=255)
                                                                                                        gagaAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCa                                          <  2:2263557/149‑1 (MQ=255)
                                                                                                         agaAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAg                                         >  1:612261/1‑149 (MQ=255)
                                                                                                           aaaCAAGTAGTTGAGTGTGAGAGCGCTGGGCTGCTTATAGAG‑A‑AAAAGTGTGTGCTGACAGATGCGTTAAATGACGTCCAAGTAGAATAAGGGGAATGTCTTTGGCTATATTGCTAAGAGCAGCATGGTAGTCAGGATTAAAATCAGtg                                       >  2:5577031/1‑148 (MQ=255)
                                                                                                           aaaCAAGTAGTTGAGTGGGAGTGTGAGAATCTCTTTTTAGAGAA‑AAAA‑TATTTGCTAACAGATGAGTTAAATCACGTCCAAGTAGAATAAGAGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAAGATTAAGATCAGtt                                       <  2:7130048/149‑1 (MQ=255)
                                                                                                           aaaCAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGtt                                       >  1:2124772/1‑149 (MQ=255)
                                                                                                             aCAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTc                                     <  2:3728596/149‑1 (MQ=255)
                                                                                                             aCAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTc                                     <  1:9362501/149‑1 (MQ=255)
                                                                                                                agtagtTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATc                                  >  1:8155628/1‑149 (MQ=255)
                                                                                                                agtagtTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATc                                  <  1:6400238/149‑1 (MQ=255)
                                                                                                                   agtTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCtaa                               >  1:7983364/1‑149 (MQ=255)
                                                                                                                     ttGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCtaata                             <  2:9557928/149‑1 (MQ=255)
                                                                                                                      tGAGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCtaataa                            <  2:3980788/149‑1 (MQ=255)
                                                                                                                        actGGGAGTGCGTTGGTCTCTTTGTAGAGAA‑AAAA‑TATTTGCGTACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTATATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAAtc                          <  2:6957757/147‑1 (MQ=255)
                                                                                                                        aGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAAtc                          >  2:4824592/1‑149 (MQ=255)
                                                                                                                        aGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAAtc                          <  1:3500799/149‑1 (MQ=255)
                                                                                                                        aGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAAtc                          >  2:2076938/1‑149 (MQ=255)
                                                                                                                        aGTGGGAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAAtc                          <  2:6717311/149‑1 (MQ=255)
                                                                                                                            ggAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAAtcctcc                      >  2:894968/1‑149 (MQ=255)
                                                                                                                            ggAGTGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAAtcctcc                      <  1:1021272/149‑1 (MQ=255)
                                                                                                                                tGCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTaaa                  <  1:1198279/149‑1 (MQ=255)
                                                                                                                                 gCGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTaaaa                 >  2:1281961/1‑149 (MQ=255)
                                                                                                                                  cGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAAt                >  1:524639/1‑149 (MQ=255)
                                                                                                                                  cGTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAAt                <  1:4655548/149‑1 (MQ=255)
                                                                                                                                   gTTGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAAtt               <  2:1807598/149‑1 (MQ=255)
                                                                                                                                    ttGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTa              <  2:2214817/149‑1 (MQ=255)
                                                                                                                                     tGGTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTaa             <  2:6600816/149‑1 (MQ=255)
                                                                                                                                      ggTCTCTTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTaaa            >  1:6027699/1‑149 (MQ=255)
                                                                                                                                          tctTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAACATTAAAACTg        >  2:2765745/1‑149 (MQ=255)
                                                                                                                                           ctTTTTAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTGc       <  2:7422509/149‑1 (MQ=255)
                                                                                                                                            tttttAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTGCa      >  2:7578748/1‑149 (MQ=255)
                                                                                                                                            tttttAGAGAA‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTGCa      <  1:2620843/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
TTCAGTCAAAATTTCTCGAAAACTATCTGTTCTCTCCTGAGTCATCCATGTATTGGACGAACCACCAATGAAAGCTAATTTCTGATAGCCTTTTTGAATCATGTGAGAAACAAGTAGTTGAGTGGGAGTGCGTTGGTCTCTTTTTAGAG‑A‑AAAA‑TATTTGCTGACAGATGAGTTAAATCACGTCCAAGTAGAATAAGGGGAATTTCTTTGGCTAAATTGCTAAGAGCAGCTTGGTAGTCAGGATTAATATCAGTTTCATCTAATAATCCTCCTAAAATTAAAACTGCATCAA  >  AM406671/1210130‑1210421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑