BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AM406671	1,614,130	+T	coding (1149/1278 nt)	mleP ←	malate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AM406671	1,614,128	1	.	T	98.8%	3185.7 / 19.5	820	coding (1151/1278 nt)	mleP	malate transporter
Reads supporting (aligned to +/- strand): ref base . (4/6); new base T (438/371); total (443/377)
Fisher's exact test for biased strand distribution p-value = 5.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 876 total aligned reads displayed.

GTTTTTTTAGGTTATGGTTATTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTG‑TT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAATTGCCATGTAAATG  >  AM406671/1613979‑1614272

 tttttttAGGTTATGGTTATTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTg                                                                                                                                                    <  1:9122602/149‑1 (MQ=255)

 tttttttAGGTTATGGTTATTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTGCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTg                                                                                                                                                    >  1:2443102/1‑149 (MQ=255)

  ttttttAGGTTATGGTTATTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTG‑t                                                                                                                                                  >  2:2687609/1‑149 (MQ=255)

    ttttAGGTTATGGTTATTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTG‑ttt                                                                                                                                                >  1:6874759/1‑148 (MQ=255)

        aGGTTATGGTTATTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTG‑tttccac                                                                                                                                            >  1:151333/1‑144 (MQ=255)

        aGGTTATGGTTATTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGACTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTG‑tttccac                                                                                                                                            >  1:5624323/1‑144 (MQ=255)

         ggttatggttatTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCAcc                                                                                                                                           >  2:1199461/1‑149 (MQ=255)

         ggttatggttatTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCAcc                                                                                                                                           >  1:6692177/1‑149 (MQ=255)

         ggttatggttatTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCAcc                                                                                                                                           <  2:7720193/149‑1 (MQ=255)

           ttatggttatTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCa                                                                                                                                         <  1:431899/149‑1 (MQ=255)

             atggttatTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATa                                                                                                                                       >  1:1776330/1‑149 (MQ=255)

              tggttatTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAg                                                                                                                                      >  1:375516/1‑149 (MQ=255)

                  tatTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAatt                                                                                                                                  >  1:4930158/1‑149 (MQ=255)

                  tatTTTAATAAAAGAATCGTATAAGAATTCCACCGAACATGAGGACAATTGCACCACAGAGTCTCTTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCGGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAatt                                                                                                                                  <  2:5365842/149‑1 (MQ=255)

                   atTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAatta                                                                                                                                 <  2:6749878/149‑1 (MQ=255)

                     tttAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAattatt                                                                                                                               >  2:9250220/1‑149 (MQ=255)

                         ataaAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATc                                                                                                                           >  2:4047982/1‑149 (MQ=255)

                            aaaGAATCGTATAAGAATTCCCCCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCCGATGCAGACAAGACAGCAATGTTACCCGTTT‑CCACCCATAGAATTATTGATCATa                                                                                                                        >  2:4979081/1‑149 (MQ=255)

                            aaaGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATa                                                                                                                        >  1:8115340/1‑149 (MQ=255)

                            aaaGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATa                                                                                                                        >  2:2813102/1‑149 (MQ=255)

                                aaTCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTg                                                                                                                    >  2:6227115/1‑149 (MQ=255)

                                 aTCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGc                                                                                                                   >  1:7617510/1‑149 (MQ=255)

                                 aTCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGc                                                                                                                   >  1:2291007/1‑149 (MQ=255)

                                  tCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCa                                                                                                                  >  1:5813266/1‑149 (MQ=255)

                                   cGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCAc                                                                                                                 <  2:3166157/149‑1 (MQ=255)

                                   cGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCAc                                                                                                                 >  1:3262726/1‑149 (MQ=255)

                                        aaGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATg                                                                                                            <  2:6487169/149‑1 (MQ=255)

                                        aaGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATg                                                                                                            <  1:2911350/149‑1 (MQ=255)

                                         aGAATTCCACCGAATATGATGACAATTGCACAACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATgg                                                                                                           <  2:868356/149‑1 (MQ=255)

                                         aGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATGATACCTGCACCAATgg                                                                                                           >  1:6467211/1‑149 (MQ=255)

                                          gAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGc                                                                                                          <  1:7360895/149‑1 (MQ=255)

                                             ttCCACCTAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGt                                                                                                       <  2:8330229/149‑1 (MQ=255)

                                             ttCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGt                                                                                                       <  1:5953541/149‑1 (MQ=255)

                                              tCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGtt                                                                                                      >  2:9150674/1‑149 (MQ=255)

                                              tCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGtt                                                                                                      <  1:9369821/149‑1 (MQ=255)

                                                 aCCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCg                                                                                                   <  1:8620716/149‑1 (MQ=255)

                                                   cGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGAc                                                                                                 <  1:2154927/149‑1 (MQ=255)

                                                   cGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGAc                                                                                                 <  1:7071940/149‑1 (MQ=255)

                                                    gAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACt                                                                                                <  2:352063/149‑1 (MQ=255)

                                                       tatGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGa                                                                                             >  1:8860507/1‑149 (MQ=255)

                                                        atGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCTATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGAt                                                                                            >  1:4125532/1‑149 (MQ=255)

                                                        atGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGAt                                                                                            >  1:1204464/1‑149 (MQ=255)

                                                        atGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGCTTCGACTGGAt                                                                                            >  2:4176966/1‑149 (MQ=255)

                                                         tGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGTAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATa                                                                                           <  2:719059/149‑1 (MQ=255)

                                                         tGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATa                                                                                           <  1:4756377/149‑1 (MQ=255)

                                                          gAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATaa                                                                                          >  2:7102581/1‑149 (MQ=255)

                                                           aGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATaaa                                                                                         >  1:1012040/1‑149 (MQ=255)

                                                             gACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAgg                                                                                       >  2:3317842/1‑149 (MQ=255)

                                                             gACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAgg                                                                                       <  2:3637911/149‑1 (MQ=255)

                                                               cAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGCCTGGATAAAGGcc                                                                                     >  1:3596875/1‑149 (MQ=255)

                                                               cAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGcc                                                                                     <  1:8376280/149‑1 (MQ=255)

                                                               cAAGTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGcc                                                                                     <  2:7338740/149‑1 (MQ=255)

                                                                aaTTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCa                                                                                    <  2:4637196/149‑1 (MQ=255)

                                                                aaTTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCa                                                                                    >  1:6785061/1‑149 (MQ=255)

                                                                  ttGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCaaa                                                                                  <  1:462038/149‑1 (MQ=255)

                                                                   tGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAag                                                                                 <  2:8168451/149‑1 (MQ=255)

                                                                       ccacAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGc                                                                             <  2:8974987/149‑1 (MQ=255)

                                                                        cacaGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCg                                                                            >  2:5902764/1‑149 (MQ=255)

                                                                        cacaGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCg                                                                            >  1:606145/1‑149 (MQ=255)

                                                                           agagTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGc                                                                         <  2:947602/149‑1 (MQ=255)

                                                                             agTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGAACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCa                                                                       >  2:2208936/1‑149 (MQ=255)

                                                                                 tATTAGCCATCTGAGCAAAAGCAATAATGTCCATTCGATCTGATGCAGAAAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGaa                                                                   <  2:158172/149‑1 (MQ=255)

                                                                                        cATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTa                                                            >  2:4472125/1‑149 (MQ=255)

                                                                                         aTCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAg                                                           <  2:1984248/149‑1 (MQ=255)

                                                                                          tCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGc                                                          >  1:1648101/1‑149 (MQ=255)

                                                                                          tCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGc                                                          >  1:4436010/1‑149 (MQ=255)

                                                                                          tCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGCTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGc                                                          >  1:7942267/1‑149 (MQ=255)

                                                                                           ccgAGCAAAAGCAATCATTTCCACTCGATCGGATGCAGACACAACAGCAAGGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCCACTGGAGAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCa                                                         >  2:8517737/3‑149 (MQ=255)

                                                                                              aGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGt                                                      >  2:3804486/1‑149 (MQ=255)

                                                                                               gCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTc                                                     >  1:3053295/1‑149 (MQ=255)

                                                                                                cAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTcc                                                    >  1:5449150/1‑149 (MQ=255)

                                                                                                cAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTcc                                                    >  1:3961298/1‑149 (MQ=255)

                                                                                                    aGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCAtt                                                >  2:6376509/1‑149 (MQ=255)

                                                                                                    aGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCAtt                                                >  1:2682270/1‑149 (MQ=255)

                                                                                                      cAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTac                                              >  1:9527362/1‑149 (MQ=255)

                                                                                                      cAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTG‑TTGCCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTac                                              <  1:873024/149‑1 (MQ=255)

                                                                                                      cAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAACGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTac                                              <  1:6975878/149‑1 (MQ=255)

                                                                                                        aTCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTacaa                                            <  1:9016187/149‑1 (MQ=255)

                                                                                                          cATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTacaaca                                          >  2:573031/1‑149 (MQ=255)

                                                                                                           atatCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTacaacaa                                         <  2:3016967/146‑1 (MQ=255)

                                                                                                               ccATTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACt                                     <  1:1111961/149‑1 (MQ=255)

                                                                                                                 aTTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTg                                   >  1:5204298/1‑149 (MQ=255)

                                                                                                                 aTTCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATCATTGCTCATACCTGCACCAATGGCAGCCTCGACTGGATACAGGCCAACGAGGCGAGCCCAGACCCAACCACCAAGCCCCATCCCAACAACACtcg                                   >  2:7898888/1‑147 (MQ=255)

                                                                                                                  ttCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGt                                  <  2:2349841/149‑1 (MQ=255)

                                                                                                                   tCGATCTGATGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTc                                 >  1:200915/1‑149 (MQ=255)

                                                                                                                           aTGCAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAg                         >  2:1341415/1‑149 (MQ=255)

                                                                                                                              cAGACAAGACAGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAATAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTac                      <  2:798975/149‑1 (MQ=255)

                                                                                                                                  caagacaGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTacaaca                  <  1:3332240/149‑1 (MQ=255)

                                                                                                                                  caagacaGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTacaaca                  >  1:6321225/1‑149 (MQ=255)

                                                                                                                                    agacaGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACaaa                >  1:8257025/1‑149 (MQ=255)

                                                                                                                                     gacaGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAAt               >  1:1346119/1‑149 (MQ=255)

                                                                                                                                        aGCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAATTGc            <  1:965445/149‑1 (MQ=255)

                                                                                                                                         gCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAATTGcc           <  2:5523111/149‑1 (MQ=255)

                                                                                                                                         gCAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAATTGcc           <  2:1755479/149‑1 (MQ=255)

                                                                                                                                          cAATGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAATTGCCa          >  1:7801807/1‑149 (MQ=255)

                                                                                                                                             tGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGTACCAATGGCAGTTTCGACTGGATAAAGGCCAATGAGGCGAGCCAAGAACCAACTAGCAAGTCTCATTACAACAACACTTGTCAAGCAAAGTACAACAAATTGCCATGt       <  2:6925006/149‑1 (MQ=255)

                                                                                                                                             tGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAATTGCCATGt       >  2:7553892/1‑149 (MQ=255)

                                                                                                                                             tGTTACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAATTGCCATGt       <  1:8481314/149‑1 (MQ=255)

                                                                                                                                               ttACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAATTGCCATGTaa     <  1:5077815/149‑1 (MQ=255)

                                                                                                                                                tACCTGTTT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAATTGCCATGTaaa    <  2:886547/149‑1 (MQ=255)

GTTTTTTTAGGTTATGGTTATTTTAATAAAAGAATCGTATAAGAATTCCACCGAATATGAGGACAATTGCACCACAGAGTCTATTAGCCATCTGAGCAAAAGCAATCATTTCCATTCGATCTGATGCAGACAAGACAGCAATGTTACCTG‑TT‑CCACCCATAGAATTATTGATCATACCTGCACCAATGGCAGTTTCGACTGGATAAAGGCCAAAGAGGCGAGCCAAGAACCAACTAGCAAGTCCCATTACAACAACACTTGTCAAGCAAAGTACAACAAATTGCCATGTAAATG  >  AM406671/1613979‑1614272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`