Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 827,811 C→T intergenic (+153/‑32) uxuA → / → uxuT D‑mannonate hydrolase/Na‑galactoside symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM406671827,8110CT99.6% 3570.2 / NA 921intergenic (+153/‑32)uxuA/uxuTD‑mannonate hydrolase/Na‑galactoside symporter
Reads supporting (aligned to +/- strand):  ref base C (1/1);  major base T (476/437);  minor base A (0/4);  total (477/444)
Fisher's exact test for biased strand distribution p-value = 5.31e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 941 total aligned reads displayed.

GTTTTATGAAGGAATCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGACAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCTGGAACAATATTGTT  >  AM406671/827665‑827956
                                                                                                                                                  |                                                                                                                                                 
  tttATGAAGGAATCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATaaaa                                                                                                                                               >  1:9407631/1‑149 (MQ=255)
  tttATGAAGGAATCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATaaaa                                                                                                                                               <  1:423461/149‑1 (MQ=255)
  tttATGAAGGAATCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATaaaa                                                                                                                                               >  2:5990766/1‑149 (MQ=255)
   ttATGAAGGAATCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATaaaaa                                                                                                                                              <  2:8437082/149‑1 (MQ=255)
    tATGAAGGAATCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAat                                                                                                                                             >  2:6393646/1‑149 (MQ=255)
     aTGAAGGAATCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAata                                                                                                                                            >  2:2683500/1‑149 (MQ=255)
      tGAAGGAATCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAatat                                                                                                                                           >  1:4380931/1‑149 (MQ=255)
      tGAAGAAATCTATTTTAAAGAATTTTTTGTGAAAAAATGTGCGGAGACATTTCTCCGCTAAATTTAGAACGAAGGAGTAAGCGTTTCACAAAGTTCACAATATAACAAAAAAAAAAAAAAAATTTGTCAATTTTCACTGATAAAAatat                                                                                                                                           <  2:556125/149‑1 (MQ=255)
       gAAGGAATCTATTTTAAAGAACTTTTTTTTTTTTTTTGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAAtata                                                                                                                                          >  2:4860245/1‑149 (MQ=255)
        aaGGAATCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATaa                                                                                                                                         >  1:3736382/1‑149 (MQ=255)
         aGGAATCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATaaa                                                                                                                                        <  1:2693214/149‑1 (MQ=255)
         aGGAATCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATaaa                                                                                                                                        >  2:9190316/1‑149 (MQ=255)
           gAATCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATaaaaa                                                                                                                                      >  1:7371598/1‑149 (MQ=255)
            aaTCTATTTTAAGTAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGataaaaatataaaaat                                                                                                                                     <  1:475109/149‑1 (MQ=255)
            aaTCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGataaaaatataaaaat                                                                                                                                     <  1:3991219/149‑1 (MQ=255)
              tCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAg                                                                                                                                   >  1:517359/1‑149 (MQ=255)
              tCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCACCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTCCACAATTTAACAAAGTTGATGGAAAAATGTGTCAATTTTCACTGATAAAAATATAAAAATAg                                                                                                                                   <  1:7839712/149‑1 (MQ=255)
               cTATTTTAAAGAACTTTTTGTTATAATATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAgg                                                                                                                                  >  2:6180241/1‑149 (MQ=255)
                tATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGga                                                                                                                                 <  1:7454877/149‑1 (MQ=255)
                 aTTTTAAAGCACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGgag                                                                                                                                <  2:7005537/149‑1 (MQ=255)
                 aTTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGgag                                                                                                                                >  2:8547851/1‑149 (MQ=255)
                   tttAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGaa                                                                                                                              <  2:2500229/149‑1 (MQ=255)
                    ttAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAAt                                                                                                                             >  1:1127416/1‑149 (MQ=255)
                     tAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATc                                                                                                                            >  1:3865771/1‑149 (MQ=255)
                      aaaGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCt                                                                                                                           <  2:1051185/149‑1 (MQ=255)
                      aaaGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCt                                                                                                                           >  1:351914/1‑149 (MQ=255)
                      aaaGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCt                                                                                                                           <  1:2080212/149‑1 (MQ=255)
                       aaGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTg                                                                                                                          >  2:4740573/1‑149 (MQ=255)
                           aCTTTTTGTGAAAAAATGTGCGGAGACTTTTATCAGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTATATAGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTa                                                                                                                      <  2:5723757/149‑1 (MQ=255)
                            cTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTaa                                                                                                                     <  2:1972320/149‑1 (MQ=255)
                             tttttGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTaaa                                                                                                                    <  1:2891458/149‑1 (MQ=255)
                              ttttGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTaaaa                                                                                                                   <  2:2834638/149‑1 (MQ=255)
                                    gAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAg                                                                                                             >  1:6378144/1‑149 (MQ=255)
                                      aaaaaTGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGaa                                                                                                           >  1:5778036/1‑149 (MQ=255)
                                          aTGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAAc                                                                                                       >  2:8234617/1‑149 (MQ=255)
                                          aTGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAAc                                                                                                       <  2:3575884/149‑1 (MQ=255)
                                          aTGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAAc                                                                                                       <  1:1859135/149‑1 (MQ=255)
                                          aTGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAAc                                                                                                       >  2:506768/1‑149 (MQ=255)
                                           tgtgCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACa                                                                                                      >  1:9563556/1‑149 (MQ=255)
                                            gtgCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACaa                                                                                                     >  1:1289332/1‑149 (MQ=255)
                                            gtgCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACaa                                                                                                     >  2:296217/1‑149 (MQ=255)
                                             tgCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACaaa                                                                                                    >  2:4171912/1‑149 (MQ=255)
                                               cGGAGCCTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATCTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACaaaaa                                                                                                  >  2:1575109/1‑149 (MQ=255)
                                               cGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACaaaaa                                                                                                  <  1:7671873/149‑1 (MQ=255)
                                               cGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACaaaaa                                                                                                  <  1:2807801/149‑1 (MQ=255)
                                                ggAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACaaaaaa                                                                                                 >  1:5983220/1‑149 (MQ=255)
                                                   gaCTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACtt                                                                                              <  1:4648113/149‑1 (MQ=255)
                                                       tttCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTg                                                                                          >  2:5895527/1‑149 (MQ=255)
                                                        ttCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTgg                                                                                         <  1:6203097/149‑1 (MQ=255)
                                                              gCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGGTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATAGCAAAAAAACAAAAAACTTCGTGGGTTTac                                                                                   <  2:3807640/149‑1 (MQ=255)
                                                               cTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTaca                                                                                  >  1:1576378/1‑149 (MQ=255)
                                                               cTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTaca                                                                                  >  1:6660448/1‑149 (MQ=255)
                                                                           cgacgGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGaaa                                                                      >  2:1180273/1‑149 (MQ=255)
                                                                             acgGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAAtt                                                                    <  2:4349924/149‑1 (MQ=255)
                                                                               ggAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTaa                                                                  <  1:3181361/149‑1 (MQ=255)
                                                                                gAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAAt                                                                 >  2:8942438/1‑149 (MQ=255)
                                                                                gAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAAt                                                                 >  2:9395761/1‑149 (MQ=255)
                                                                                 aGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATa                                                                <  1:8270013/149‑1 (MQ=255)
                                                                                      gCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAgg                                                           >  1:8491200/1‑149 (MQ=255)
                                                                                        gTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCg                                                         <  2:6825673/149‑1 (MQ=255)
                                                                                         tttCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGt                                                        >  1:6854334/1‑149 (MQ=255)
                                                                                         tttCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGt                                                        >  1:2216299/1‑149 (MQ=255)
                                                                                          ttCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTg                                                       >  2:6561192/1‑149 (MQ=255)
                                                                                             acaAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGtc                                                    <  1:4905544/149‑1 (MQ=255)
                                                                                             acaAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGtc                                                    >  1:7082698/1‑149 (MQ=255)
                                                                                             acaAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGtc                                                    <  1:8031512/149‑1 (MQ=255)
                                                                                                 aGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGtcttct                                                >  1:5703013/1‑149 (MQ=255)
                                                                                                   ttCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCttt                                              <  1:1456743/149‑1 (MQ=255)
                                                                                                   ttCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCttt                                              <  1:329249/149‑1 (MQ=255)
                                                                                                     cacaATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTAc                                            >  1:9249976/1‑149 (MQ=255)
                                                                                                      acaATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGAAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACg                                           <  2:7110579/149‑1 (MQ=255)
                                                                                                        aaTTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGc                                         >  1:7173434/1‑149 (MQ=255)
                                                                                                        aaTTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGc                                         >  1:9063370/1‑149 (MQ=255)
                                                                                                         aTTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCt                                        >  1:8138736/1‑149 (MQ=255)
                                                                                                           ttAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTa                                      >  2:3980688/1‑149 (MQ=255)
                                                                                                           tgaaCAAAGTTTATGGAAAAATTTGTAAATTTTCAATGATAAAAATAAAAAAATAGGAGAAACAGTAAAAAAAAGAAAAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTa                                      <  2:3203789/147‑1 (MQ=255)
                                                                                                            tAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTaa                                     >  2:2435064/1‑149 (MQ=255)
                                                                                                            tAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTaa                                     >  1:1918703/1‑149 (MQ=255)
                                                                                                               cAAAGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGAAAAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCg                                  <  2:5326338/149‑1 (MQ=255)
                                                                                                                aaaGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGa                                 >  1:9154254/1‑149 (MQ=255)
                                                                                                                 aaGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGAt                                >  2:4494447/1‑149 (MQ=255)
                                                                                                                 aaGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGAt                                <  1:8640252/149‑1 (MQ=255)
                                                                                                                 aaGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGAt                                >  1:2417770/1‑149 (MQ=255)
                                                                                                                  aGTTTATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGAtt                               >  2:8789983/1‑149 (MQ=255)
                                                                                                                      tATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAAt                           <  1:506768/149‑1 (MQ=255)
                                                                                                                      tATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAAt                           >  1:2522352/1‑149 (MQ=255)
                                                                                                                      tATGGAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACAAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAAt                           >  1:100900/1‑149 (MQ=255)
                                                                                                                        tGGAAAAATTTGTCAATAAAGAAAAATAAAAAAATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATgg                         <  2:2261601/149‑1 (MQ=255)
                                                                                                                          gAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGc                       <  1:4118887/149‑1 (MQ=255)
                                                                                                                          gAAAAATTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGAGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGc                       <  2:3491920/149‑1 (MQ=255)
                                                                                                                             aaaTTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGa                    >  2:7393451/1‑149 (MQ=255)
                                                                                                                               aTTTGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAGTACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAgg                  <  1:5264660/149‑1 (MQ=255)
                                                                                                                                 ttGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCt                <  2:7905033/149‑1 (MQ=255)
                                                                                                                                  tGTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCTg               >  1:1705889/1‑149 (MQ=255)
                                                                                                                                   gTCAATTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCTgg              <  1:55092/149‑1 (MQ=255)
                                                                                                                                       aTTTTCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCTGGAACa          >  1:302227/1‑149 (MQ=255)
                                                                                                                                         tttCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCTGGAACAat        >  2:760864/1‑149 (MQ=255)
                                                                                                                                           tCACTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCTGGAACAatat      >  1:8897710/1‑149 (MQ=255)
                                                                                                                                             aCTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCTGGAACAATATTg    >  2:1838699/1‑149 (MQ=255)
                                                                                                                                             aCTGATAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGAAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCTGGAACAATATTg    <  2:801808/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GTTTTATGAAGGAATCTATTTTAAAGAACTTTTTGTGAAAAAATGTGCGGAGACTTTTCTCCGCTAAATTTAGAACGACGGAGTAAGCGTTTCACAAAGTTCACAATTTAACAAAGTTTATGGAAAAATTTGTCAATTTTCACTGACAAAAATATAAAAATAGGAGAATCTGTTTAAAATGGCAGAAAAACAAAAAACTTCGTGGGTTTACACACCAAAAGAAATTAATACAAGGCGTGGTCTTCTTTACGGCTTAACCGATTTAATGGGCGGAGGCTGGAACAATATTGTT  >  AM406671/827665‑827956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑