Predicted mutation
evidence seq id position mutation annotation gene description
RA AM406671 912,618 T→A L46Q (CTA→CAA)  llmg_0944 → putative secreted protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM406671912,6180TA99.7% 3433.4 / NA 880L46Q (CTA→CAA) llmg_0944putative secreted protein
Reads supporting (aligned to +/- strand):  ref base T (1/1);  major base A (463/410);  minor base C (3/0);  total (469/411)
Fisher's exact test for biased strand distribution p-value = 2.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 884 total aligned reads displayed.

AGGAAAATAAATGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCTAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAATTTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTA  >  AM406671/912472‑912763
                                                                                                                                                  |                                                                                                                                                 
 ggAAAATAAATGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAAc                                                                                                                                                >  1:8939631/1‑149 (MQ=255)
   aaaaTAAATGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAg                                                                                                                                              <  1:658398/149‑1 (MQ=255)
   aaaaTAAATGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAg                                                                                                                                              >  1:8641794/1‑149 (MQ=255)
   aaaaTAAATGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAg                                                                                                                                              <  2:3045681/149‑1 (MQ=255)
    aaataaatGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCGCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAAAAGa                                                                                                                                             >  2:8931994/1‑149 (MQ=255)
       taaatGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGataa                                                                                                                                          <  2:8449188/149‑1 (MQ=255)
            gAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTg                                                                                                                                     <  2:8829870/149‑1 (MQ=255)
              aTTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGGAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGGATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTgca                                                                                                                                   >  2:8216788/1‑149 (MQ=255)
                   agaaaaagaaaaACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCaaa                                                                                                                              >  2:3998761/1‑149 (MQ=255)
                      aaaagaaaaaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAAtt                                                                                                                           >  1:3106833/1‑149 (MQ=255)
                      aaaagaaaaaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAAtt                                                                                                                           >  1:4380179/1‑149 (MQ=255)
                       aaagaaaaaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAAttt                                                                                                                          <  2:8070635/149‑1 (MQ=255)
                        aagaaaaaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTta                                                                                                                         <  2:2208481/149‑1 (MQ=255)
                        aagaaaaaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTta                                                                                                                         <  2:7501865/149‑1 (MQ=255)
                          gaaaaaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTtata                                                                                                                       <  2:4985895/149‑1 (MQ=255)
                          gaaaaaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTtata                                                                                                                       >  1:2578753/1‑149 (MQ=255)
                          gaaaaaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTtata                                                                                                                       >  1:1548763/1‑149 (MQ=255)
                          gaaaaaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTtata                                                                                                                       >  1:6863372/1‑149 (MQ=255)
                            aaaaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATAcc                                                                                                                     <  2:37850/149‑1 (MQ=255)
                             aaaCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCa                                                                                                                    >  1:943675/1‑149 (MQ=255)
                               aCAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGa                                                                                                                  <  2:5871173/149‑1 (MQ=255)
                                cAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGaa                                                                                                                 >  1:8143072/1‑149 (MQ=255)
                                  gagaAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAAtc                                                                                                               >  1:5507237/1‑149 (MQ=255)
                                   agaagaAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAAtct                                                                                                              >  2:2722655/1‑149 (MQ=255)
                                      agaaACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCaa                                                                                                           >  1:3263516/1‑149 (MQ=255)
                                      agaaACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCaa                                                                                                           <  2:4517357/149‑1 (MQ=255)
                                         aaCGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAAc                                                                                                        <  1:1008174/149‑1 (MQ=255)
                                          aCGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGGAGCAAAATTTATACCAGAATCTCAAAACt                                                                                                       >  2:5644820/1‑149 (MQ=255)
                                                agGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATGTTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACgg                                                                                                 <  1:6984863/149‑1 (MQ=255)
                                                agGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACgg                                                                                                 <  2:3575209/149‑1 (MQ=255)
                                                 gggggTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGa                                                                                                <  1:6425059/149‑1 (MQ=255)
                                                   gggTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATg                                                                                              <  2:1203796/149‑1 (MQ=255)
                                                    ggTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGc                                                                                             <  1:2345829/149‑1 (MQ=255)
                                                     gTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCa                                                                                            >  1:5160439/1‑149 (MQ=255)
                                                      tAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAg                                                                                           <  2:9152187/149‑1 (MQ=255)
                                                        aaGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAATAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCg                                                                                         >  1:7922784/1‑149 (MQ=255)
                                                        aaGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCg                                                                                         >  1:8343650/1‑149 (MQ=255)
                                                         aGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCgg                                                                                        >  1:7213369/1‑149 (MQ=255)
                                                          gTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGa                                                                                       >  2:687579/1‑149 (MQ=255)
                                                              ttGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCaa                                                                                   >  1:6764788/1‑149 (MQ=255)
                                                               tGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCaaa                                                                                  >  2:6468650/1‑149 (MQ=255)
                                                                 cAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAAtt                                                                                >  2:624416/1‑149 (MQ=255)
                                                                 cAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAAtt                                                                                <  1:4583564/149‑1 (MQ=255)
                                                                  aaTATTTCTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTg                                                                               <  2:3164872/149‑1 (MQ=255)
                                                                    tatTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGt                                                                             <  1:3524616/149‑1 (MQ=255)
                                                                    tatTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGt                                                                             <  1:9160324/149‑1 (MQ=255)
                                                                     atTACTCTTCGTTATTTTAGTTGTGGCTCTGATTGTTTTTGATATTGTAATGATAGTACGACACGTTCTTTCTTGTCAAACAGATAATATTGCAGCTAAATTTATACGAGAACCTCAATACTTAACGGATGCAGCGGATAAAATTAGtt                                                                            >  2:329737/1‑149 (MQ=255)
                                                                         ctctCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGc                                                                        >  2:7066079/1‑149 (MQ=255)
                                                                          tctcCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCt                                                                       <  1:5635021/149‑1 (MQ=255)
                                                                             cccTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACt                                                                    <  2:5455317/149‑1 (MQ=255)
                                                                             cccTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACt                                                                    <  1:7810437/149‑1 (MQ=255)
                                                                                ttattaTATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCg                                                                 >  2:1871009/1‑149 (MQ=255)
                                                                                 tattatATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGt                                                                >  1:4819180/1‑149 (MQ=255)
                                                                                  attatATTTCTGGCTCTGATTGTTTTTGNTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTc                                                               >  1:5344755/1‑149 (MQ=255)
                                                                                  attatATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGAAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACCCCGTc                                                               >  1:400012/1‑149 (MQ=255)
                                                                                   ttatATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCa                                                              >  1:2819730/1‑149 (MQ=255)
                                                                                   ttatATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCa                                                              >  1:1922057/1‑149 (MQ=255)
                                                                                      tatTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAACTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCt                                                           <  2:6074108/149‑1 (MQ=255)
                                                                                        tttCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTAt                                                         >  2:7601739/1‑149 (MQ=255)
                                                                                            tGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTgca                                                     <  1:2243116/149‑1 (MQ=255)
                                                                                              gCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTgcaag                                                   <  1:6211144/149‑1 (MQ=255)
                                                                                               ctctGATTGTTTTTTTTTTTTTTTTGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCACCATCTCAAAACTTAACGGATGCAGCGGATCATATTGGTTCGGCTACTCCGTCAGCTATTgctagc                                                  >  2:2361853/1‑149 (MQ=255)
                                                                                                tctGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAACTTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTgcaagca                                                 >  2:7328362/1‑149 (MQ=255)
                                                                                                 ctGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTgcaagcaa                                                <  1:5222868/149‑1 (MQ=255)
                                                                                                  tGATTGTTTTTTTTTTTTTTTTGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGTATCAAATTGGTTCGGCTACTCCGTCAGCTATTgcaagcaaa                                               >  2:1364262/1‑148 (MQ=255)
                                                                                                     ttGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTa                                            >  1:7537405/1‑149 (MQ=255)
                                                                                                     ttGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTa                                            >  1:5941507/1‑149 (MQ=255)
                                                                                                      tGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTaa                                           >  1:1151150/1‑149 (MQ=255)
                                                                                                        ttttttttATTTTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAAt                                         >  2:8341267/1‑149 (MQ=255)
                                                                                                        tttttGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAAt                                         >  1:1324668/1‑149 (MQ=255)
                                                                                                        tttttGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAAt                                         <  2:8740763/149‑1 (MQ=255)
                                                                                                         ttttGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATg                                        <  2:9327693/149‑1 (MQ=255)
                                                                                                          tttGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATga                                       <  2:7204683/149‑1 (MQ=255)
                                                                                                           ttGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATgag                                      >  2:2124766/1‑149 (MQ=255)
                                                                                                           ttGGTATTGTAATGATAGGACGACACATTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATgag                                      <  2:2497459/149‑1 (MQ=255)
                                                                                                            tGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATgaga                                     >  1:2028150/1‑149 (MQ=255)
                                                                                                            tGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATgaga                                     <  2:7774752/149‑1 (MQ=255)
                                                                                                            tGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCAGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATgaga                                     >  2:405299/1‑149 (MQ=255)
                                                                                                              gTATTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAAt                                   <  1:6112103/149‑1 (MQ=255)
                                                                                                              gTATTGGAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAAt                                   <  2:4352162/149‑1 (MQ=255)
                                                                                                                aTTGTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTc                                 <  1:7422781/149‑1 (MQ=255)
                                                                                                                   gTAATGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGa                              <  2:5296349/149‑1 (MQ=255)
                                                                                                                     aaTGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATa                            >  1:1769227/1‑149 (MQ=255)
                                                                                                                      aTGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATAc                           <  2:6588819/149‑1 (MQ=255)
                                                                                                                      aTGATACGACGACACGTTATTTCTAGTCAAACAGATAATCTTGCAGCAAAATTTATACCAGAATCTCAAAACATAACGGATGCAGCGGAACAAATTGGTTCGGCTACTCCGTCAGCTATTGCCAGCAAAGTAAATGAGAAATCGGATAc                           >  2:4698689/1‑149 (MQ=255)
                                                                                                                       tGATAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTGAAAACATAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATAcc                          >  2:1022952/1‑149 (MQ=255)
                                                                                                                         aTAGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCAt                        <  1:5737338/149‑1 (MQ=255)
                                                                                                                           aGGACGACACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCa                      >  1:4954945/1‑149 (MQ=255)
                                                                                                                             gacgacACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAag                    <  1:2728719/149‑1 (MQ=255)
                                                                                                                              acgacACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAaga                   >  2:8574944/1‑149 (MQ=255)
                                                                                                                                gacACGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGt                 >  2:7893341/1‑149 (MQ=255)
                                                                                                                                 acacGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTc                >  2:2929105/1‑149 (MQ=255)
                                                                                                                                  cacGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCa               <  1:3006140/149‑1 (MQ=255)
                                                                                                                                  cacGTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGGCa               >  1:414078/1‑149 (MQ=255)
                                                                                                                                     gTTATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAAtc            <  2:1971861/149‑1 (MQ=255)
                                                                                                                                      ttATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAAtct           <  1:7676349/149‑1 (MQ=255)
                                                                                                                                       tATTTCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAAtctc          >  2:1567100/1‑149 (MQ=255)
                                                                                                                                         tttCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAg        <  1:3767501/149‑1 (MQ=255)
                                                                                                                                          ttCTAGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAgg       <  2:9204512/149‑1 (MQ=255)
                                                                                                                                              aGTCAAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAACGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAAATTAAATGAGAATTCGGATAGCATCAAGAGTCAAATCCAGGGAtt   >  2:1686596/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AGGAAAATAAATGAATTCTAGAAAAAGAAAAACAGAGAAGAAACGCAGAGGGGGTAAAGTATTTGCAATATTACTCTCCCTTATTATATTTCTGGCTCTGATTGTTTTTGGTATTGTAATGATAGGACGACACGTTATTTCTAGTCTAACAGATAATATTGCAGCAAAATTTATACCAGAATCTCAAAACTTAACGGATGCAGCGGATCAAATTGGTTCGGCTACTCCGTCAGCTATTGCAAGCAATTTAAATGAGAATTCGGATACCATCAAGAGTCAATCTCAGGGATTA  >  AM406671/912472‑912763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑